• 폴리머
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• 제32권2호
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• pp.131-137
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• 2008

전기방사법에 의해 폴리스테렌 나노섬유 부직포를 제조하였고, 간세포의 접착을 유도하기 위해 간세포접착 리간드인 Poly[N-p-vinylbenzyl-O-$\beta$-D-galactopyranocyl-($1{\rightarrow}4$)-D-gluconamide](PVLA)를 나노섬유의 표면에 코팅하였다. 얻어진 나노섬유는 평균직경이 500 nm이었고 분광학적인 방법을 통해 PS 나노섬유 표면의 개질 여부를 확인하였다. 장기간 배양시의 간세포 생존율을 MTT법으로 조사한 결과 기존의 세포배양접시에서는 급격하게 세포생존율이 감소함에 비해 PVLA가 코팅된 나노섬유상에서는 생존율이 서서히 감소하였으며 약 3주간에 걸쳐 간세포가 생존율이 유지되었다. 간세포의 대표적인 기능인 알부민분비 및 요소분해능을 측정한 결과 기존의 세포배양접시에서는 급격하게 간세포기능이 저하되었지만, PVLA가 코팅된 나노섬유상에서는 약 3주정도 초기의 간세포기능을 유지하는 것이 밝혀졌다.

• KSBB Journal
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• 제29권1호
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• pp.58-66
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• 2014

Demand for in vitro pharmacological evaluation and toxicity test using human hepatocytes has been increasing. In USA and Europe, human hepatocytes obtained from donated whole liver unsuitable for transplantation were distributed to researchers and deposited in cell bank facility as cryopreserved vial. In Korea, however, incidence of transplantation- inappropriate whole liver has been quite low and the whole livers almost have so severe liver disease such as fatty or fibrotic liver that cannot meet the demand. In this study we aimed to isolate human hepatocytes from liver resection surgery-originated partial liver, and assure the isolated human hepatocytes and its cryopreserved hepatocytes to be qualified for the in vitro pharmacological evaluation and drug toxicity tests. We compared those with commercially available human hepatocyte, BD $GenTest^{TM}$ by cell morphology, hepatic gene expression, urea synthesis, albumin secretion, ammonia removal, and cytochrome P450 induction activities. Changes in hepatotoxic gene expression after cryopreservation are evaluated with a typical hepatotoxic drug, acetaminophen. Consequently, the fresh hepatocytes from the partial liver and its cryopreserved hepatocytes expressed their intrinsic hepatic functions well and showed equal hepatotoxicity gene expression trend regardless to cryopreservation. Therefore, liver resection surgery-originated partial liver can be used as a useful source of human hepatocytes for various pharmacological and hepatotoxicity test.