• 한국초지조사료학회지
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• 제33권4호
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• pp.240-244
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• 2013

본 연구는 추청벼를 이용하여 총체 벼 사일리지를 조제하였을 때 총체 벼 사일리지의 사료가치 및 부위별 소화율을 조사하여 총체 벼의 이용성을 검정하기 위하여 수행되었다. 총체 벼 사일리지의 무기물 함량은 망간이 가장 높았으며 탄소, 철, 아연, 구리 등 순으로 나타났다. 그리고 마그네슘 함량이 가장 낮게 나타났다. 추청벼 총체 사일리지의 아미노산은 glutamic acid 함량이 가장 높았으며 leucine, asparagine, alanine, valine, arginine 순으로 나타났다. 그리고 methionine 함량이 가장 낮았다. 추청벼 총체 사일리지 부위(총체 벼, 낟알을 제거한 줄기, 왕겨를 포함한 낟알, 쌀)에 따른 시간별 반추위내 건물 소화율은 쌀이 가장 높았으며 왕겨를 포함한 낟알, 총체 벼, 낟알을 제거한 줄기 등 순으로 나타났다. 따라서 본 연구에서는 추청벼 총체 사일리지 소화율은 부위에 따라 약간의 차이를 보였으며 무기물 중 망간 그리고 아미노산 중 glutamic acid가 가장 높은 함량을 보였다.

• 한국수산과학회지
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• 제12권4호
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• pp.225-234
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• 1979

자원이 풍부한 해조류 단백질의 효율적인 이용과 이의 영양가를 구명하기 위하여 가장 효과적인 정제방법 및 추출용매를 결정하고 4종 식용해조 즉 둥근 돌김, 구멍갈파래, 미역 및 모자반의 추출 정제단백질의 아미노산 조성과 이의 영양가 및 소화율을 실험한 결과를 요약하면 다음과 같다. 1. 홍조류와 녹조류는 추출단백질을 TCA로 심전시킨 후 ethylether로 TCA를 충분히 제거하면 정제효과가 좋았고, 갈조류는 등전점을 이용한 정제방법의 효과가 좋았다. 2. 각종 용매로 추출한 단백질의 아미노산을 분석한 결과 홍조류와 녹조류는 물 또는 알칼리 추출한 단백질이 좋았고, 갈조류는 알코올-알칼리 혼합용매로 추출한 단백질이 좋았고, 3. 둥근돌김의 정제단백질은 lyeine의 함량이 전시료 중에서 가장 높았으며 구멍갈파래는 threonine의 함량이 월등히 높은 반면, lysine의 함량이 낮았으며 미역과 모자반의 아미노산 분포 양상은 둥근돌김과 구멍갈파래보다 좋지 못했다. 4. 둥근돌김과 구멍갈파래 원조분말의 chemical score는 35정도였으나 정제단백질은 56정도있고, 미역과 모자반은 원조분말이 $10\~16$정도로 극히 낮은 반면에 정제단백질은 $30\~35$ 정도를 나타내었고, protein score는 전시료가 정제단백질의 경우 $62\~73$ 정도를 나타내었다. 5. PPDRI를 이용한 소화율은 원조분말의 $25\~40$ 정도의 낮은 소화율을 보였으나 정제했을 경우에는 $1.3\~2.1$배 증가함을 보였고, 갈조류의 소화율은 일반적으로 극히 낮음을 알 수 있었다.

• 한국수산과학회지
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• 제46권6호
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• pp.769-776
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• 2013

This study evaluated fermented soybean meal (FSM) as a fish meal (FM) replacement and determined the appropriate amount of FSM in juvenile olive flounder diet. Twenty-four aquaria with a flowing-water system were stocked with fish averaging 20.9 g at a density of 25 fish/tank. Five experimental diets were prepared replacing FM with 0, 10, 20, 30, or 40% FSM based on FM protein (designated $FSM_{0}$, $FSM_{10}$, $FSM_{20}$ $FSM_{30}$, and $FSM_{40}$, respectively). Two additional diets were prepared that replaced 30 or 40% of the FM with FSM with added amino acids (methionine and lysine) (designated $FSM_{30+AA}$, and $FSM_{40+AA}$, respectively). Fish (triplicates) were fed one of the eight experimental diets (50% crude protein and 16.7 kJ available energy $g^{-1}$ diet) for 8 weeks. Survival did not differ among the treatments during the feeding experiment. There were no significant differences in weight gain (WG) or specific growth rate (SGR) among the fish fed diets with up to 30% of the FM replaced. However, fish fed $FSM_{40}$ or $FSM_{40+AA}$ had a reduced WG and SGR, as compared to $FSM_0$ (control) (P < 0.05). The feed efficiency and apparent digestibility showed a similar trend (P < 0.05). The proximate composition in the whole body of fish differed only between the control and $FSM_{40}$ for the crude protein level and between the control and $FSM_{30+AA}$ for the crude lipid level. The whole-body amino acid composition did not differ among treatments. No significant differences were found between the diet groups with and without amino acid supplementation, indicating that amino acid supplementation had no effect. The major finding of this study is that fermented soybean meal may replace up to 30% of fish meal without amino acid supplementation for normal growth of juvenile olive flounder.

• Journal of Microbiology and Biotechnology
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• 제1권3호
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• pp.212-219
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• 1991

Four species of yeast, Saccharomyces cerevisiae, Candida utilis, Saccharomycopsis flbuligera, and Schwanniomyces castellii were evaluated for their ability to bioconvert potato processing waste water into microbial protein and the resulting single-cell proteins were evaluated as protein sources for rainbow trout, using in vitro analyses. The studies indicated that Schwanniomyces castellii, which utilizes starch dircetly and converts it into cell mass efficiently, was suitable for the bioconversion. In the single-stage continuous bioconversion, the yield S. castellii cell mass, which contained approximately 37% protein, was 77%, at dilution rate 0.25 $h^{-1}$. Reduction of total carbohydrate was 81%. During batch fermentations, cell mass yield was about 72% and total carbohydrate reduction was 81%. Among the yeasts tested, S. castellii possessed the most fragile cell wall and had a favorable amino acid profile for salmonid fish; protein score of 86% (Met). In an in vitro pepsin digestibility test 80% digestibility (23~38% above control) was observed when cells were pre-heated in a steam bath for 30 min. Results presented should be regarded as being preliminary in nature because they were derived from single experiments.

• Fisheries and Aquatic Sciences
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• 제14권4호
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• pp.257-265
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• 2011

The effects of cooking method (grilling, frying, steaming, and microwaving) on the proximate composition and protein quality of chub mackerel Scomber japonicus treated with 2, 6, and 10% sodium chloride (NaCl) brine were investigated. Moisture content decreased in all cooked samples from 60.22% in the raw sample to 48.7% in the fried samples. Brine (10% NaCl) treatment recorded the highest moisture loss. All cooked samples showed a decrease in fat content, except fried samples. Protein content increased in all cooked samples, from 47.21% in the raw sample to 63.87% in the grilled sample. Brine treatment resulted in the highest degree of fat oxidation (thiobarbituric acid-reactive substances), which was highest in the fried samples and lowest in the microwaved samples. The trypsin inhibitor (TI) concentration was highest in the microwaved samples and lowest in the fried samples. In all samples, 6% salt treatment caused the lowest TI level and the highest in vitro protein digestibility. In vitro digestibility increased from 79.4% in the raw sample to 86.43% in the fried samples. The total essential amino acids of all cooked samples increased. Results suggested that grilling and steaming had beneficial effects on the protein quality of chub mackerel.

• 농업과학연구
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• 제51권1호
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• pp.53-61
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• 2024

A total of 180 21-day-old weaning pigs ([Yorkshire × Landrace] × Duroc) with an initial body weight of 6.44 ± 0.01 kg were randomly assigned to 9 treatments for evaluating the effects of replacing dietary inorganic copper (Cu) and zinc (Zn) with glycine (Gly) or methionine (Met)-chelated Cu and Zn on growth performance and nutrient digestibility. The experimental period was 35 days. There were four replicated pens per treatment, with five pigs (three males and two females) per pen. Dietary treatments consisted of a basal diet (CON), in which the sources of Cu and Zn were in inorganic form. The inorganic Cu and Zn in the basal diet were replaced by glycine-chelated (GC) and methionine-chelated (MC) Cu and Zn by 30, 50, 70, or 100% to form the GC1, GC2, GC3, GC4, or MC1, MC2, MC3, MC4 groups. The 100% replacement of dietary inorganic Cu and Zn with GC or MC increased (p < 0.05) average daily gain, average daily feed intake, and gain-to-feed ratio. The complete replacement of dietary inorganic Cu and Zn with GC or MC led to enhanced (p < 0.05) digestibility of dry matter, nitrogen, Cu and Zn. Thus, the replacement of inorganic Cu and Zn with GC or MC can improve the growth efficiency and nutrient utilization of weaning pigs.

• Asian-Australasian Journal of Animal Sciences
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• 제24권1호
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• pp.88-95
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• 2011

An experiment was conducted to determine the standardized ileal digestibility (SID) of amino acids (AA) in four sources of full-fat soybeans (FFSB) and in one source of soybean meal (SBM). The FFSB had different concentrations of trypsin inhibitor units (TIU) and included two sources of conventional FFSB, and two sources of a soybean variety that was selected for a reduced concentration of the Kunitz trypsin inhibitor. The conventional FFSB was either low temperature-processed (LT-FFSB-CV; 37.7% CP, 35.4 TIU/mg) or high temperature-processed (HT-FFSB-CV; 40.5% CP, 4.4 TIU/mg). The low-Kunitz FFSB was also either low temperature-processed (LT-FFSB-LK; 36.2% CP, 23.5 TIU/mg) or high temperature-processed HT-FFSB-LK; (38.2% CP, 4.0 TIU/mg). The SBM contained 47.5% CP and 3.20 TIU/mg. Twelve weanling barrows (initial BW: $11.1{\pm}1.3\;kg$) were fitted with a T-cannula in the distal ileum. Pigs were allotted to a replicated $6{\times}6$ Latin square design with six diets and six periods per square. Five diets were prepared using each of the soybean sources as the only source of AA in the diet. An N-free diet was also included in the experiment to measure basal endogenous losses of AA. The two low temperature-processed FFSB had lower (p<0.05) AID and SID values for all indispensable AA than the two high temperature-processed FFSB and SBM. The SID values for all indispensible AA except Trp were greater (p<0.05) in LT-FFSB-LK than in LT-FFSB-CV, but the SID of AA in HT-FFSB-CV and HT-FFSB-LK were not different. The SID of AA in SBM were not different from the SID in HT-FFSB-CV and in HT-FFSB-LK. Results of this experiment show that a reduction of the TIU from 35.4 to 23.5 TIU/mg will improve the SID of AA, but this reduction is not sufficient to completely ameliorate the negative impact of trypsin inhibitors. Results also show that the SID of AA in high temperature-processed FFSB is similar to that in de-hulled SBM.

• Asian-Australasian Journal of Animal Sciences
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• 제18권9호
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• pp.1285-1293
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• 2005

The present study was conducted to evaluate and compare the effects of various animal and plant protein sources on piglet' performance, digestibility of amino acids and gut morphology in weaned pigs until 28 days after weaning. The plant protein sources used were soybean meal (SBM), fermented soy protein (FSP), rice protein concentrate (RPC); and animal protein sources tested were, whey protein concentrate (WPC) and fishmeal (FM). Iso-proteinous (21%) diets were formulated and lysine (1.55%) content was similar in all the diets. The level of each protein source added was 6% by replacing SBM to the same extent from the control diet containing 15% SBM. The ADG was higher (p<0.05) in the groups fed animal proteins as compared with plant proteins at all the levels of measurement, except during 15-28 days. The highest ADG was noted in WPC and FM fed diets and lowest in SBM fed diet. The feed intake was higher in animal protein fed groups than plant proteins at all phases, but the feed:gain ratio was not affected by protein sources except during overall (0 to 14 day) measurement which was improved (p<0.05) in animal protein fed diets compared to plant protein sources. The digestibilities of gross energy, dry matter and crude protein were higher in animal protein fed groups than for plant protein fed sources. The apparent ileal digestibilities of essential amino acids like Leu, Thr, and Met were significantly (p<0.05) higher in animal proteins fed animals as compared with plant protein fed animals. But the apparent fecal digestibilities of essential amino acids like Arg and Ile were significantly higher (p<0.05) in plant protein diets than animal protein sources. The villous structure studied by scanning electron microscope were prominent, straight finger-like, although shortened and densely located in FM fed group as compared with others. The lactic acid bacteria and C. perfringens counts were higher in caecal contents of pigs fed plant proteins than the animal proteins. Overall, it could be concluded that animal protein sources in the present study showed better effects on growth performance, nutrient digestibility and gut morphology than plant protein sources.

• Asian-Australasian Journal of Animal Sciences
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• 제14권7호
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• pp.994-1002
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• 2001

In a balance experiment with rats either 0, 25 or 50% of the crude protein (CP) provided as casein in the control diet was replaced with cassava leaves (CL) (Manihot esculenta Crantz) or sweet potato vines (SPV) (Ipomoea balala). CL were either sun-dried or oven-dried at $60^{\circ}C$ or $105^{\circ}C$ or ensiled, while the SPY were either sun-dried or ensiled. The experiment included 3 blocks with 30 rats in each and six individuals per treatment group. Drying at $105^{\circ}C$ resulted in a reduction of the lysine (Lys) content, suggestive of the occurrence of Maillard reactions. Ensiling CL and SPV slightly decreased the CP. content as well as the sum of essential amino acids. The apparent fecal CP digestibility (dCP) and nitrogen retention were negatively affected by increasing the level of replacement (p<0.01 and p<0.001, respectively). The impaired amino acid profile observed when drying CL at $105^{\circ}C$ was found to be related to a slight decrease in dCP (p<0.001) as well as N retention (p<0.005). The effects of sun-drying and oven-drying in reducing the HCN content in CL were more potent than when ensiling. By increasing the total dietary HCN supply serum thiocyanide level, as well as urinary thiocyanate and linamarin output, were increased, with a weak relationship between them. Sun-drying and ensiling with cane molasses as additive successfully preserved the nitrogenous constituents and could be a means of preserving fresh green feed under tropical conditions.

• Asian-Australasian Journal of Animal Sciences
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• 제11권1호
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• pp.89-96
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• 1998

The experiment was conducted with 120 barrows weaned at 21 days of age to estimate their lysine requirement weaned at 21 days of age when other important amino acids were fortified to get optimal ratio to lysine. The treatments were 1.15% (control), 1.25%, 1.35%, 1.45%, 1.55%, 1.65% total lysine in the diet. Based on the growth performance total lysine requirement of 21-day old pigs appears to be 1.45%. The lowest digestibilities of dry matter and crude fat were found in pigs fed 1.15% total lysine diet and the highest were found in pigs fed 1.65% total lysine diet with no significant differences among treatments. Nitrogen digestibility increased as the total lysine level increased up to 1.35% (p < 0.05) and remained relatively constant beyond 1.35%. However, the best nitrogen digestibility was observed in pigs fed 1.45% total dietary lysine. Gross energy, crude ash and phosphorus digestibilities did not differ due to the increase in total lysine level. The amounts of excreted dry matter and nitrogen differed significantly by the increase in lysine level up to 1.35% (p < 0.05), while phosphorus excretion was not influenced by the lysine level. Dry matter and nitrogen excretion were reduced by 13.6% and 18.4%, respectively, when 1.45% lysine was offered to the pigs compared to the those fed on 1.15% lysine diet. The amino acid digestibilities increased as the total lysine level increased up to 1.45% (p < 0.05), and remained constant beyond 1.45%. The lysine requirement for the pigs weighing 6 to 14 kg seems to be higher than the previous estimates and in order to reduce pollutant excretion the accurate nutrient requirement should be set and applied to the animal.