• Title/Summary/Keyword: Amaranthus mangostanus

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Purification and Characterization of Antiviral Protein (AAP29) from the Leaves of Amaranthus mangostanus (참비름 (Amaranthus mangostanus)에서 항바이러스성 단백질 (AAP29)의 분리 및 특성)

  • Yi, Seung-In;Kim, Yeong-Tae;Hwang, Young-Soo;Cho, Kang-Jin
    • Applied Biological Chemistry
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    • v.38 no.6
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    • pp.528-533
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    • 1995
  • An antiviral protein (AAP29) with ribosome-inactivating activity was purified and characterized from the leaves of the Amaranthus mangostanus. Purification was accomplished through crude extraction, ammonium sulfate precipitation, S-Sepharose chromatography, gel filtration, CM-Sepharose chromatography and Blue sepharose chromatography. This protein was about 29.2 kDa and strongly basic with the PI value between 9.0 and 9.6, indicating that AAP29 is similar to Type 1 RIP. The AAP29 showed high thermostability without activity toss even after 20 min at $50^{\circ}C$. In cell free system using rabbit reticulocyte lysate, AAP29 inhibited protein synthesis with an $IC_{50}$, of 0.18 nM. This protein also reduced mosaic symptoms of cucumber mosaic virus (CMV) on tobacco leaves. The N-terminal amino acid sequences of the AAP29 are ADLTFTVTKDGTSQSYXTLXNXWRXW and shows no sequence similarity with any known RIPs.

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Volatile flavor components of Ixeris dentata and Amaranthus mangostanus (씀바귀와 비름의 휘발성 풍미성분)

  • Kim, Mi-Kyung;Lee, Mie-Soon
    • Applied Biological Chemistry
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    • v.31 no.4
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    • pp.394-399
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    • 1988
  • Volatile components of Ixeris dentata and Amaranthus mangostanus, Korean wild vegetables, were collected by Steam Distillation-Extraction(SDE) method. Essential oils were analyzed by gas chromatography(GC)and combined gas chromatography-mass spectrometry(GC-MS). Sixty one components, including 13 alcohols, 9 esters, 12 aldehydes, 6 ketones, 9 hydrocarbons, 6 acids, 3 phenols and 3 miscellaneous ones were confirmed in Ixeris dentata. Fifty six components, including 15 alcohols, 5 esters, 13 aldehydes, 8 ketones, 3 hydrocarbons, 9 acids and 5 miscellaneous ones were confirmed in Amaranthus mangostanus.

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Anthracnose of Amaranthus mangostanus Caused by Glomerella cingulata in Korea (Glomerella cingulata에 의한 비름 탄저병)

  • Kwon, Jin-Hyeuk;Park, Chang-Seuk
    • The Korean Journal of Mycology
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    • v.31 no.1
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    • pp.40-43
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    • 2003
  • Symptoms similar to anthracnose were observed on Amaranthus mangostanus in Sancheon-gun, Gyeongnam province, where the plants were autogenously formed community. The symptoms were appeared in stem and spread, eventually whole plants died. Mycelial colony of the isolate was whitish gray to dark gray on potato dextrose agar. Conidia were single celled, colorless, cylindrical and measured as $10.5{\sim}21.7{\times}3.8{\sim}6.0{\mu}m$. Appressoria were dark brown, ovate to obovate and sized as $5.6{\sim}13.7{\times}4.6{\sim}11.4{\mu}m$. Perithecia were brown to black in color and shaped as globose to obpyriform and sized as $79.7{\sim}286.7{\mu}m$. Asci had eight ascospores and sized as $47.7{\sim}89.7{\times}8.1{\sim}13.3{\mu}m$. Ascospores were slightly curved at the center cylindrical, fusiform and measured $9.3{\sim}20.3{\times}4.6{\sim}6.3{\mu}m$. Optimum temperature for growth was $30^{\circ}C$. On the basis of morphological characteristics and pathogenicity test to host plants, the fungus was identified as Glomerella cingulata. This is the first report on the Anthracnose of Amaranthus mangostanus caused by Glomerella cingulata in Korea.

Inhibition of Tobacco Mosaic Virus Infection by the Crude Sap Extracted from Amaranthaceae Plants (비름과식물즙액에 의한 담배 모자이크 바이러스의 감염억제효과)

  • Choi Jang-Kyung;Jung Ok-Hoa
    • Korean journal of applied entomology
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    • v.23 no.3 s.60
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    • pp.137-141
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    • 1984
  • Crude sap, which was extracted from six Amaranthaceae plants, inhibited local lesion formation on Nicotiana glutinosa by tobacco mosaic virus(TMV) infection. Especially the remark. able inhibitory effect to TMV infection was shown on leaves of N. glutinosa precoated with the sap from Amaranthus mangostanus. The inhibitory activity of the sap from A. mangostanus was stable to storage in vitro for I day and to dilution 1/4 of the sap with distilled water. However, its activity was lost when the sap was heated at $70^{\circ}C\;to\;100^{\circ}C$ for 10 minutes. When the leaves of N. glutinosa precoated with the sap were sprayed with water, the inhibitory effect to TMV infection was maintained for 2 days. The A. mangostanus sap readjusted pH 3, pH 5, or pH 9 with 1 N HCl or 1 N NaOH did not decline the inhibitory action but the sap absorbed with $5\%\;to\;15\%$ charcoal completely lost their action. The protein components purified from A. mangostanus sap revealed three major bands by $5\%\;to\;15\%$ polyacrylamide gel electrophoresis and the top component of which showed the inhibitory action to TMV infection.

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Damping-off of Edible Amaranth Caused by Rhizoctonia solani AG-4

  • Kim, Wan-Gyu;Shim, Hong-Sik;Lee, Gyo-Bin;Cho, Weon-Dae
    • The Korean Journal of Mycology
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    • v.48 no.3
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    • pp.325-328
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    • 2020
  • Damping-off symptoms were frequently observed on young plants of edible amaranth (Amaranthus mangostanus) grown in vinyl greenhouses by farmers located in Goyang and Yangpyeong in Korea during disease surveys carried out in 2019 and 2020. The incidence of the disease was 1-20%. A total of eight isolates of Rhizoctonia sp. were obtained from the stems of the diseased plants. All the isolates were identified as Rhizoctonia solani AG-4, based on the morphological characteristics and anastomosis test. Three isolates of R. solani AG-4 were tested for pathogenicity on edible amaranth by artificial inoculation. All the tested isolates of R. solani AG-4 induced damping-off symptoms on the inoculated plants; these symptoms were similar to those observed on the vinyl greenhouse plants surveyed. This is the first report of R. solani AG-4 causing damping-off in edible amaranth.

Screening of Sterol Biosynthesis Inhibitors from Natural Products Using Recombinant Yeast Carrying Human Lanosterol Synthase

  • Sung, Chung-Ki;Kim, Eun-A;Chu, Yun-Ho;Shibuya, Masaaki;Ebizuka, Yutaka
    • Natural Product Sciences
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    • v.9 no.4
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    • pp.299-303
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    • 2003
  • For the screening of inhibitors of sterol biosynthesis from natural products, a simple and rapid assay method was developed using recombinant yeast carrying human lanosterol synthase, main target of this assay method. Sterol biosynthesis inhibition activity was monitored only by the inhibition of growth of the recombinant yeast. By changing the substrate, this assay method can figure out which step is inhibited in the sterol biosynthesis by the test material. With this assay method total 102 plant samples were screened for their inhibitory activity of sterol biosynthesis. Among plant water extracts screened, 11 plant samples showed inhibitory activity on sterol biosynthesis in ergosterol (-) medium. For selection of the specific inhibitory materials, 11 plant samples were reassayed in ergosterol (+) medium. After all 5 plant samples, Abutilon avicennae Gaertn. (stem), Alnus japonica Steud. (stem), Amaranthus mangostanus L. (aerial part), Philadelphus schrenckii Pupr. (leaf) and Pimpinelia brachycarpa Nakai (aerial part), showed specific inhibitory activity.

Occurrence and Distribution of C4 Plants under Diverse Agricultural Field Types in Korea (농업생태계에서 농경지유형에 따른 C4식물의 출현과 분포)

  • Cho, Kwang-Jin;Oh, Young-Ju;Kang, Kee-Kyung;Han, Min-Su;Na, Young-Eun;Kim, Miran;Choe, Lak-Jung;Kim, Myung-Hyun
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.15 no.2
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    • pp.85-101
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    • 2013
  • In order to search for distribution characteristics on C4 plants at the paddy fields, uplands, orchard and roadside in South Korea, vascular plants were investigated. Flora investigation had been carried out from May 2002 to October 2006 at 241 plots. In the results of survey, the flora of these areas consist of 74 families, 231 genera and 352 species totally. The class frequencies were arranged by the order of Magnoliopsidae (255 species), Monocotyledoneae (89 species), Pteropsida (7 species) and Sphenopsida (1 species). The number of species in each investigated agricultural field types was 55 families with 203 species in paddy field, 49 families with 218 species in uplands, 44 families with 115 species in orchard and 48 families with 202 species in roadside respectiviely. Representative species compositions of these four agricultural field types were characterized by Artemisia princeps, Persicaria thunbergii in paddy field, Artemisia princeps, Erigeron annuus in upland and roadside, Commelina communis, Digitaria ciliaris in orchard, respectively. Therefore, indicative species in agricultural ecosystem was Artemisia princeps. Among the investigated 352 species, $C_4$ plants identified were 38 species, and the occurrence ratio of $C_4$ monocotyledonous plants were higher in paddy field, orchard and roadside than upland. Such differences in the occurrence ratio of $C_4$ plants under diverse agricultural field types reflect differences in environmental condition such as micro-climate, soil moisture under various agricultural fields. Dominant $C_4$ monocotyledonous plants were Digitaria ciliaris and Commelina communis, while $C_4$ dicotyledonous plants were Amaranthus mangostanus and Chenopodium album var. centrorubrum in agricultural fields. Naturalized plants were identified as 47 species and occurrence ratio were higher in upland and roadside than other agricultural field types. Among the investigated naturalized plants, $C_4$ plants were Amaranthus mangostanus and Amaranthus retroflexus. Distribution characteristics of representative $C_4$ plants in agricultural field types, revealed that the projected increase in temperature due to climate change may provide better conditions for the growth of $C_4$ plants. Thus, the necessity of long-term monitoring should be conducted on the diffusion of $C_4$ plants that may threaten influence agroecosystem in Korea.

Effect of Application Rates of Mineral N and Cattle Slurry on the Dry Matter Yield and Botanical Compositions of Orchargrass Sward (무기태 질소와 액상구비의 시용수준이 Orchargrass 초지의 건물수량과 식생구성에 미치는 영향)

  • 이주삼;김성준;권진욱
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.15 no.1
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    • pp.43-51
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    • 1995
  • Tlis experiment was to study the effects of application rates of mineral N and cattle slurry on the dry matter yield and botanical compositions of Orchardgrass sward grown in 4 cuttings in a year. Annual rates of mineral N of O($N_0$), 120kg(($N_1$), 240kg(($N_2$) and 360kg/ha(($N_3$) were applied as urea, and cattle slurry applied at rates of O(($N_0=N_0$), $40m^3$($S_1$), $80m^3$(($S_2$) and $120m^3$($S_3$), suppling 120kg, 240kg and 360kgl ha, respectively. The results were summarized as follows; 1. The limiting annual application rates of mineral N and cattle slurry was estimated about 120kg/ ha. 2. The growth of Orchardgrass after the 3rd cut was decreased by summer depression. Consequently, the botanical compositions of Orchardgrass showed only 7.7% and 4.0% in 3rd and 4th cut, respectively. However, the 15 and 17 species of weeds originated in 3rd and 4th cut. 3. The botanical compositions of Amaranthus mangostanus in 3rd cut and Portulacea aleracea in 4th cut were greatly increased with application rates of cattle sluny. 4. Relative efficiency of cattle sluny for the dry matter production of Orchardgrass as compared to mineral N were 93.396, 99.5% and 113.6% in 120kg, 240kg and 360kglha at rates of cattle slurry, respectively.

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Weed Flora of Ulreung Island in Korea (울릉도(鬱陵島)에 발생(發生)하는 잡초종(雜草種))

  • Kim, K.U.;Kwon, S.T.;Lee, I.J.;Kim, S.H.
    • Korean Journal of Weed Science
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    • v.7 no.3
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    • pp.265-279
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    • 1987
  • About 140 weed species belonging to 42 families occurring in summer were observed in Ulreung island. The Compositae was the most wildely occurring family covering 27 weed species, followed by 21 species in Graminae, 9 in Polygonaceae, 7 in Leguminosae, and 7 in Labiatae etc. In terms of the lands classified, about 60 species in 26 families were observed in the cultivated and the medical crops grown areas, respectively, and 116 species in 40 families occurred in the non-cultivated land like the vicinity of the cultivated area and 94 species in 34 families in the valley. No. of species and families were much greater in the non-cutivated land than those of the cultivated one. The most dominant weed species in both the cultivated and its vicinity in Ulreung island were Digitaria sanguinalis, followed by Portulaca oleracea, Polygonum hydropiper, Equisetum arvensis, Artemisia princeps, Commetina communis, Setaria viridis in order. Community analysis was done by the method of Toyohara in two cultivated lands such as the general crop land and the medical herb crop grown land, and two non-cultivated areas such as the vicinity of the cultivated land and valley. The cultivated land consisted of the communites of P. hydropiper and Cyperus amuricus, including Bidens tripartita in P. hydropiper community. The medical crop grown land composed of three major communities like P. hydropiper, Amaranthus mangostanus and Sonchus asper in which A. mangostanus and S. media were presented in the ecoton, indicating community being transiting. In the non-cultivated areas like the vicinity of crop land, the communities of Erigeron annuus, P. oleracea, and Oxalis corniculata were dominant, and Dystaenia takeshimana was included in the community of E. annuus and Stellan'a aquatica in P. oleracea community. In the valley, Ranunculus quelpaertensis community was existed in between the communities of Sonchus asper and Plantago asiatica.

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