• 한국균학회지
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• 제44권4호
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• pp.233-239
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• 2016

A survey of the indigenous fungal resources of Korea was undertaken during 2014-2015. All specimens collected in this study were identified at the species level, based on their morphological characteristics and rDNA sequence data. Among them, 12 macrofungal species, viz. Agaricus guizhouensis, Amanita orientifulva, Armillaria cepistipes, Crepidotus inhonestus, Daldinia childiae, Elmerina cladophora, Lycoperdon scabrum, Marasmius brunneoaurantiacus, Otidea bufonia, Pluteus hongoi, Pluteus variabilicolor, and Russula grisea have not been previously reported in Korea.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2003년도 춘계 학술발표대회
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• pp.119-120
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• 2003

Many fungi were collected at Mt. Daedun Provincial Park between the periods from March 2001 to October 2002 and they were identified. According to the results, the fungi diversity included 2 division, 3 subdivisions, 5 classes, 4 subclasses, 12 orders, 35 families, 75 genera and 153 species. Among them Ileodictyon is unrecorded genus and Ileodictyon gracile, Mycena flosum-nivium and Tricholoma lascivum are newly to Korea. Dominant family are Tricholomataceae, Russulaceae and Polyporaceae;genus Amanita and species Collybia confluens and Russula emetica In useful fungi and ecological resources, edible fungi included 51 species; culture fungi 10; poisonous fungi 18: medicinal fungi 17: anticancer fungi 22: ectomycorrhizal fungi 26 and wood rotting fungi 51, In geographical distributions of Chonbuk areas, same species are 50 spcies of Jeongup ; 56 of Mt.Manduck : 84 of Mt.Jiri : 28 of Pyonsan national park : 59 of Cheonju arboretum and 51 of Mt,Sunun.

• 한국균학회지
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• 제42권4호
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• pp.344-348
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• 2014

A total of 118 fungal specimens were collected from Mt. Manggyeong, Chungnam province, Korea between July and October 2014. All specimens were identified to the species level using a combination of morphological characteristics and DNA sequencing. Out of 118 specimens, eight species, Amanita manginiana, A. pilosella, Calvatia holothurioides, Cantharellus tabernensis, Dicephalospora rufocornea, Gymnopus menehune, Marasmius brunneospermus, and Russula cerolens, have not been previously reported in Korea.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2014년도 춘계학술대회 및 임시총회
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• pp.19-19
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• 2014

This study included the analysis of mushroom data collected from Mt. Chiak in Gangwon-do using various methods. Former studies of Korean mushrooms are limited by regional characters and there is less species diversity among the regions. This study tried to find a way for the forecast of mushroom distribution and appearance by indexes of species diversity. The indexes used in this study include the number of fungi (N), the number of species (S), similarity index (C), richness index (R1, R2), variety index (V1, V2), evenness index (E1, E2, E3, E4, E5), and dominance index (D1) to analyze variety of species diversity. Analyses of data of fungi using a multistage cluster sampling indicate that the average value of C for years was higher than the average value of C for areas. The mushrooms consisted of 208 species in 686 individuals in limited fungal collection from 2002 to 2003. One hundred thirty nine species in 393 individuals were collected in 2002, and 122 species 293 individuals were collected in 2003. The individuals collected in 2003 were smaller than 2002's individuals. Similarity, richness, and variety indexes' values of 2003 were reduced than 2002's values but dominance index of 2003 was increased than 2002's value. Generally the species diversity of the environment to evaluate the index of similarity, richness, and variety was a higher index; dominance index was lower than that of the surrounding environment, suggesting a good diversity. As a result, the occurrence of mushrooms in the surrounding environment and the various factors seem fell in 2002 compared to 2003. The majority genus of the limited fungal collection was Mycena genus in 63 individuals; the majority species was Laccaria laccata in 34 individuals. Ninety three species in 106 individuals were collected by the extended collection and the majority genus of the extended collection was Amanita genus in 17 individuals; the majority species was Amanita citrina (Schaeff.) Pers. which was found in 5 individuals. This demonstrates that periodical similarity's value was 0.159 is higher than special similarity's 0.119. This indicates that the probability of the appearance of same mushrooms in the same area in following year is higher than the probability of the appearance of same mushrooms in the surrounding area in same year. The value of coefficient of variation (CV), in which the amount of change is much or less by N is higher than the CV value by S. CV value of dominance index(D) was the highest r point among other indexes, and evenness index (E) was the lowest point among other indexes. The correlation matrix with 66 combinations between the indexes, the combinations with correlations was 46 combinations. These results revealed that indexes of R1, V2, and E1 were proper to represent species diversity of fungi based on the correlation matrix and the theory of statistical independence which means there is no or less mutual association. This research would contribute to the study about variable living creature by measuring method and in the future this would be used to figure out regulation about fungi with their correlation, values in ecosystem, develop improving new models about agricultural fungi species and numbers by investigating agricultural variable species.

• 한국균학회지
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• 제32권2호
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• pp.71-78
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• 2004

2000년 6월부터 2001년 10월까지 식재지별로 외생균근성 버섯을 조사한 결과는 다음과 같았다. 조사기간 동안 총 3강 3아강 8목 22과 41속 72종 (2변종 포함)의 버섯이 조사되었다. 버섯은 주름버섯목에 9과 21속 48종, 민주름버섯목에 5과 11속 13종, 이형담자균강에 3과 3속 4종, 복균아강에 5과 6속 7종이었다. 외생균근성 버섯은 총 7과 11곡 30종 2,451개체가 조사되었다. 버섯 개체수는 색시졸각버섯(1,225) 긴골광대버섯아재비(179), 자주졸각버섯(130)의 순으로 조사되었다. 외생균근성 버섯 발생은 기후환경요인과 밀접한 관계가 있는 7월과 8월에 집중하였다. 외생균근성 버섯의 종수 및 개체수 분포는 조사구별로 큰 차이가 있었으며, 이는 각 조사구별 입지환경 및 기주 수종 구성 등의 차이 때문인 것으로 사료되었다. 색시졸각버섯은 참나무속에서만 발생하여 기주선택성이 매우 좁고, 긴골광대버섯아재비, 수원무당버섯, 땀버섯속은 침엽수 및 활엽수 모두에서 버섯이 발생되어 기주선택성이 넓은 것으로 나타났다. 외생균근성 버섯의 분포에 영향을 주는 환경은 토양환경 중 토양 pH, 유기물함량 및 전질소함량과 기후환경인 상대습도가 중요한 인자로 나타났다.

• 한국버섯학회지
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• 제3권4호
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• pp.145-153
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• 2005

미생물을 이용한 중금속 오염지의 생물적 개량연구를 위하여 구리, 납 등 중금속 오염지인 은성 폐광과 도곡 폐광지역에서 야생버섯을 수집 동정하고 중금속 함량을 분석한 결과 Laccaria laccata가 가장 높게 나타났으며 토양 내 중금속함량이 높을수록 버섯 내 중금속 농도도 높았다. 그러나, 전이계수를 이용하여 버섯 자실체의 중금속 이행률을 알아본 결과, Amanita volvata가 카드뮴과 비소의 이행률이 가장 높았으며, 구리의 이행률은 Agaricus praesquamosus가 다른 버섯에 비해 월등히 높은 수치를 나타냈다. 납은 Mycena pura, 아연은 Marasmius pulcherripes, 니켈은 Laccaria laccata, 크롬은 Collybia confluens로 나타났다. 중금속 오염지와 비오염지인 치악산 지역에서의 담자균의 발생 분포를 비교한 결과, Russula와 Collybia 속은 비오염지와 오염지역 모두에서 발생되었으며 Leucocoprinus, Coprinus, Suillus, Lepiost, Gyroporus. Lepista, Microstoma, Stropharia, Agrocybe 속은 오염지에서만 발생되었다. 이상의 결과, Laccaria laccata 등 일부 담자균은 중금속 오염지 저감에 이용할 가능성이 있으며 이를 위한 많은 연구가 기대된다.

• 한국균학회지
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• 제26권3호통권86호
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• pp.306-313
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• 1998

대한민국 홍천의 송이 발생림에서 외생균근성 버섯의 발생상에 대하여 조사하였다. 송이 균환은 3년간$(1995{\sim}1997)$의 송이 자실체 발생위치를 토대로 파악하였고, 다른 외생균근성 버섯의 출현 위치는 x축과 y축의 좌표를 표시하며 2년간$(1996{\sim}1997)$ 조사하였다. 송이 균환의 직경은 $2{\sim}10m$의 범위를 나타내어, 송이 균은 대체로 연간 10 cm 정도 생장하는 것을 감안할 때 균환의 연령은 $5{\sim}50$년에 달하는 것으로 여겨졌다. 무당버섯(Russula bella), 회갈색무당버섯(R. sororia), 푸른주름무당버섯(R. delica), 애기꾀꼬리버섯(Cantharellus minor)이 조사기간에 주로 출현한 버섯이었는데 그 출현빈도는 전체 조사된 종류의 각각 16.0%, 12.8% 12.4% 및 7.0%를 차지하고 있었다. 조사자료를 분석한 결과, 연구대상지는 송이의 생산량이 감소하는 숲으로 여겨졌다. 또한, 마귀광대버섯(Amanita pantherina), 황소비단그물버섯(Suilllus bovinus), 다박싸리버섯(Ramaria flaccida) 및 졸각버섯(Laccaria amethystea)은 송이의 균환주변에 많이 출현하여 송이 생산성이 감소하는 상태에 있음을 나타내는 지표종으로 여겨겼다.

• 한국균학회지
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• 제45권4호
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• pp.319-327
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• 2017

In this study, we investigated the culture conditions of four ectomycorrhizal fungi, namely, Amanita spissacea NIFoS 2719, Pisolithus arhizus NIFoS 2784, Suillus spraguei NIFoS 2848, and Xanthoconium affine NIFoS 2716, in solid and liquid culture media. In addition, we attempted to induce in vitro mycorrhization of the fungi with Pinus densiflora. Prior to liquid culture, we determined the optimal culture conditions for each species in solid media. The results revealed that all species examined are capable of growth in potato dextrose agar (PDA), malt extract agar (MEA), and modified Melin-Norkran's medium (MMN), although their preferred growth media were different. Liquid culture experiments showed that inorganic nitrogen did not enhance the mycelial growth of all four species. Therefore, we used MMN-based liquid inocula to promote the growth of ectomycorrhizal fungi in our symbiosis culture system. Mycorrhization was observed in Xanthoconium affine NIFoS 2716. Morphological analysis revealed that fungi-inoculated roots of P. densiflora form simple and dichotomous lateral roots with dense mycelia. In addition, inoculation with X. affine NIFoS 2716 promoted root and shoot developments.

• 대한의생명과학회지
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• 제13권3호
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• pp.245-249
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• 2007

We investigated the fibrinolytic and ${\alpha}-glucosidase$ inhibitory activity of 55 wild mushroom methanol extracts. Among them, 14 mushrooms showed fibrinolytic activity. In particular, Amanita virgineoides showed the greatest enzyme activity (3.9 plasmin units/ml) by a fibrin plate assay. The fibrinolytic activities of Suillus pictus and Polypolellus varius were 3.8 plasmin units, and the activity of Gomphus fujisanensis was 2.8 plasmin units. Leccinum extremiorientale and Xerocomus nigromaculatus had the same activity with 2.3 plasmin units. In a ${\alpha}-glucosidase$ inhibitory activity test, Lactarius sp. showed the greatest inhibitory activity at 97.3%. The ${\alpha}-glucosidase$ inhibitory activities of Clitocybe odora, Xerocomus nigromaculatus, Melanoleuca melaleuca, Suillus pictus, and Gyroporus castaneus were 84.3%, 77.9%, 74.6%, 68.7%, and 65.4%, respectively. According to the results, because Suillus pictus and Xerocomus nigromaculatus have strong fibrinolytic and ${\alpha}-glucosidase$ inhibitory activities, the two mushrooms will be used as materials for the development of new biofunctional food.

• Mass Spectrometry Letters
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• 제12권3호
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• pp.112-117
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• 2021

α-Amanitin and β-amanitin are highly toxic bicyclic octapeptides responsible for the poisoning of poisonous mushrooms such as Amanita, Galerina, and Lepiota by inhibiting RNA polymerase II, DNA transcription, and protein synthesis. A sensitive, simple, and selective liquid chromatography-high resolution mass spectrometric method using parallel reaction monitoring mode was developed and validated for the simultaneous determination of α- and β-amanitin in mouse plasma to evaluate the toxicokinetics of α- and β-amanitin in mice. Protein precipitation of 5 μL mouse plasma sample with methanol as sample clean-up procedure and use of negative electrospray ionization resulted in better sensitivity and less matrix effect. The calibration curves for α- and β-amanitin in mouse plasma were linear over the range of 0.5-500 ng/mL. The intra- and inter-day coefficient of variations and accuracies for α- and β-amanitin at four quality control concentrations were 3.1-14.6% and 92.5-115.0%, respectively. The present method was successfully applied to the toxicokinetic study of α- and β-amanitin after an oral administration of α- and β-amanitin at 1.5 mg/kg dose to male ICR mice.