• The Korean Journal of Physiology and Pharmacology
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• v.27 no.2
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• pp.157-165
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• 2023

Triple-negative breast cancer (TNBC) is the most aggressive subtype of breast cancer and current therapeutic strategies are limited in their effectiveness. The expressions of Rab5 and the M2 tumor-associated macrophage marker CD163 in tissues were detected by Western blot. The migration and invasion of cells were determined using a Transwell assay. The expressions of the exosome markers were evaluated by Western blot. The polarization of human macrophages (THP-1) was determined by incubation of THP-1 cells with conditioned medium or exosomes collected from MDA-MB-231 cells with indicated transfections or by a coculture system of THP-1 and MDA-MB-231 cells. The M1 and M2 macrophage markers were evaluated by qRT-PCR. The expression of Rab5 in TNBC was significantly higher than that in normal breast tissue. Rab5 expressions in triple-negative and luminal A breast cancer were higher than those in other molecular subtypes. Higher CD163 expression was observed in triple-negative breast cancer and in triple-negative and luminal B subtypes. Rab5 knockdown suppressed but Rab5 overexpression promoted the migration and invasion capacity of MDA-MB-231 cells. The levels of CD63 and CD9 in the medium of Rab5 knockdown cells were lower than those in control cells, whereas higher levels of CD63 and CD9 were observed in Rab5 overexpression cells. Rab5 knockdown decreased the excretion but did not alter the diameter of the exosomes. Knockdown of Rab5 facilitated the anti-tumor polarization of macrophages, which was partially reversed by Rab5 overexpression. Therefore, Rab5 is expected to be a potential therapeutic target for triple-negative breast cancer.

• International Journal of Computer Science & Network Security
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• v.23 no.10
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• pp.97-106
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• 2023

Sentiment analysis using social network platforms such as Twitter has achieved tremendous results. Twitter is an online social networking site that contains a rich amount of data. The platform is known as an information channel corresponding to different sites and categories. Tweets are most often publicly accessible with very few limitations and security options available. Twitter also has powerful tools to enhance the utility of Twitter and a powerful search system to make publicly accessible the recently posted tweets by keyword. As popular social media, Twitter has the potential for interconnectivity of information, reviews, updates, and all of which is important to engage the targeted population. In this work, numerous methods that perform a classification of tweet sentiment in Twitter is discussed. There has been a lot of work in the field of sentiment analysis of Twitter data. This study provides a comprehensive analysis of the most standard and widely applicable techniques for opinion mining that are based on machine learning and lexicon-based along with their metrics. The proposed work is helpful to analyze the information in the tweets where opinions are highly unstructured, heterogeneous, and polarized positive, negative or neutral. In order to validate the performance of the proposed framework, an extensive series of experiments has been performed on the real world twitter dataset that alter to show the effectiveness of the proposed framework. This research effort also highlighted the recent challenges in the field of sentiment analysis along with the future scope of the proposed work.

• International Journal of Advanced Culture Technology
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• v.11 no.4
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• pp.177-192
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• 2023

A prerequisite for a lasting ceasefire is the emergence of a prevailing view in Moscow and Kyiv that the fighting has reached a hurting stalemate. In sum, they both lose more through continuing warfare than by a ceasefire. This study applies social identity dynamics of nationalism to this escalatory conflict. It generates findings that imply that China as a third-party great power intervening mediator can potentially play a pivotal role. Shifting the respective prevailing views in Moscow and Kyiv of their interaction from a zero-sum foundation requires proffering powerful economic and political third-party incentives. Effective inducement would facilitate national defense, development and prestige for Moscow as well as Kyiv. China arguably has the underutilized potential power capabilities necessary to alter the respective prevailing views of strategic relationships among the great powers within Moscow, Brussels and Washington. A prerequisite for success in striving effectively towards this strategic goal is cooperation with the Beijing despite skepticism from Washington. This study utilizes a process tracing methodological approach. It highlights that the foundations of the Russo-Ukraine war lie in the institutionalization within Euro-Atlantic integration of the Cold War assumption that the USSR was an imperialist revisionist actor. Russia is the USSR's successor state. Moscow's prevailing view is that Russian national self-determination was unjustly circumscribed in the multinational Soviet totalitarian Communist system. The Euro-Atlantic community is perceived as a neocolonial imperial threat by allying with post-1991 Ukrainian nationalism at Russia's expense. The study finds that acknowledging Eurasian regional multipolarity is necessary, if not sufficient, to coopt Beijing into a global political stabilization strategy. It functionally aims to promote international balancing to lessen potentials for horizontal as well as vertical escalation of the Russo-Ukrainian conflict.

• Journal of Animal Reproduction and Biotechnology
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• v.39 no.2
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• pp.138-144
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• 2024

Background: As the number of households raising companion dogs increases, the pet genetic analysis market also continues to grow. However, most studies have focused on specific purposes or native breeds. This study aimed to collect genomic data through single nucleotide polymorphism (SNP) chip analysis of companion dogs in South Korea and perform genetic diversity analysis and SNP annotation. Methods: We collected samples from 95 dogs belonging to 26 breeds, including mixed breeds, in South Korea. The SNP genotypes were obtained for each sample using an Axiom^TM Canine HD Array. Quality control (QC) was performed to enhance the accuracy of the analysis. A genetic diversity analysis was performed for each SNP. Results: QC initially selected SNPs, and after excluding non-diverse ones, 621,672 SNPs were identified. Genetic diversity analysis revealed minor allele frequencies, polymorphism information content, expected heterozygosity, and observed heterozygosity values of 0.220, 0.244, 0.301, and 0.261, respectively. The SNP annotation indicated that most variations had an uncertain or minimal impact on gene function. However, approximately 16,000 non-synonymous SNPs (nsSNPs) have been found to significantly alter gene function or affect exons by changing translated amino acids. Conclusions: This study obtained data on SNP genetic diversity and functional SNPs in companion dogs raised in South Korea. The results suggest that establishing an SNP set for individual identification could enable a gene-based registration system. Furthermore, identifying and researching nsSNPs related to behavior and diseases could improve dog care and prevent abandonment.

• Korea journal of population studies
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• v.23 no.2
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• pp.113-141
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• 2000

The aim of this paper is to project the state of the labor farce and employment in Korea from 2000 to 2005. The labor market in Korea is experiencing significant changes with the rapid development of Information and Telecommunication Technology (ICT) and the transition of the Korean economy into a knowledge-based economy. On the labor supply side, it is expected that the growth of the labor force will be sluggish; baby boomers will become the middle-aged, while the proportion of senior citizens, the highly educated and the female labor force will grow fast. These changes will alter the human resources management system in business sectors. Moreover, the permanent employment relationship, the hierarchy system and the seniority-based wage system are all expected to change. On the labor demand side, the employment share in highly skilled. knowledge-intensive industries will grow faster than the rest of the economy in tandem with the quickly growing output share of these industries. Especially, more jobs will be created in the ICT industries. The proportion of labor in highly skilled and professional occupations will also grow faster than in other occupations. At the same time, the employment share of female workers will grow more quickly than that of the male workers. These changes, however, may worsen income inequalities and/or increase the unemployment rate when workers do not have the suitable skills or knowledge required by the knowledge-based economy. To avoid this, it is necessary for the government to build up a lifetime learning system for workers.

• Journal of Korea Foundry Society
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• v.43 no.6
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• pp.302-309
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• 2023

In general, castings often have complex shapes and significant variations in thickness within a single product, making grid generation for simulations challenging. Casting flows involve multiphase flows, requiring the tracking of the boundary between air and molten metal. Additionally, considerable time is spent calculating pressure fields due to density differences in a numerical analysis. For these reasons, the Cartesian grid system has traditionally been used in mold filling simulations. However, orthogonal grids fail to represent shapes accurately, leading to a momentum loss caused by the stair-like grid patterns on curved and sloped surfaces. This can alter the flow of molten metals and result in incorrect casting process designs. To address this issue, simulations in the Cartesian grid system involve creating a large number of grids to represent shapes more accurately. Alternatively, the Cut Cell method can be applied to address the problems arising from the Cartesian grid system. In this study, analysis results based on the number of grid in the Cartesian grid system for a casting flow analysis were compared with results obtained using the Cut Cell method. Casting flow simulations of actual products during various casting processes were also conducted, and these results were analyzed with and without applying the Cut Cell method.

• Journal of the Korea Concrete Institute
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• v.17 no.1 s.85
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• pp.85-94
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• 2005

Post-tensioned Precast concrete System(PPS) consists of U-shaped precast wide beams and concrete column. The continuity of beam-column joint is provided with floor concrete cast on the PC shell beam and post-tensioning. The purpose of this paper is to evaluate the response of PPS interior beam-column joint subjected to cyclic lateral loading. To this end, an experimental investigation was performed with three half-scale specimens of interior connection. The design parameters are the amount of beam reinforcement placed inside the joint core. The test results showed that cracks were distributed well without my significant degradation of strength and ductility. Also, it was found that the prestressing may affect to alter the torsional crack angle. And the specimens sufficiently resist up to limiting drift ratio of 0.035 in accordance with the provisional by ACl of acceptance criteria for concrete special moment frames.

• Development and Reproduction
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• v.15 no.3
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• pp.265-272
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• 2011

It is well known that adipose tissue or body fat has been proved as a crucial component of brain-peripheral axis which can modulate the activities of reproductive hormonal axis in female mammals including rodents and human. Concerning the male reproduction, however, the role of adipose tissue has not been thoroughly studied. The present study was carried out to elucidate the effect of a high-fat (HF) diet on the reproductive system of postpubertal male rats. The HF diet (45% energy from fat, HF group) was applied to male rats from week 8 after birth for 4 weeks. The blood glucose levels, body and tissue weights were measured. Histological studies were performed to assess the structural alterations in the reproductive tissues. To determine the transcriptional changes of reproductive hormone-related genes in hypothalamus and pituitary, total RNAs were extracted and applied to the semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). Body weights (p<0.01) and blood glucose levels (p<0.01) of HF group were significantly higher than those of control animals. Similarly, the weights of epididymis (p<0.05), prostate (p<0.01), seminal vesicle (p<0.01) in HF group were higher than control levels. The weights of testis were not changed. The weights of kidney (p<0.001) and spleen (p<0.01) were significantly higher than control levels while the adrenal and pancreas weights were not changed. There were only slight alterations in the microstructures of accessory sex organs; the shape of luminal epithelial cells in epididymis from HF group were relatively thicker and bigger than those from control animals. In the semi-quantitative RT-PCR studies, the mRNA levels of hypothalamic GnRH (p<0.05) in HF group were significantly higher than those from the control animals. The mRNA levels of kisspeptin in HF group tend to be higher than control levels, the difference was not significant. Unlike the hypothalamic GnRH expression, the mRNA levels of pituitary $LH{\beta}$ and $FSH{\beta}$ were significantly decreased in HF group (p<0.05). The present study indicated that the 4-weeks feeding HF diet during the postpubertal period can alter the hypothalamus-pituitary (H-P) neuroendocrine reproductive system These results suggest that the increased body fat and the altered leptin input might disturb the H-P reproductive hormonal activities in male rats, and the changed activities seem to be responsible for the changes of tissue weights in accessory sex organs.

• Proceedings of the Korean Society of Medical Physics Conference
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• 2002.09a
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• pp.248-251
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• 2002

The intensity modulated radiation therapy (IMRT) with a multileaf collimator (MLC) requires the conversion of a radiation fluence map into a leaf sequence file that controls the movement of the MLC during radiation treatment of patients. Patient dose verification is clinically one of the most important parts in the treatment delivery of the radiation therapy. The three dimensional (3D) reconstruction of dose distribution delivered to the target helps to verify patient dose and to determine the physical characteristics of beams used in IMRT. A new method is presented for the pretreatment dosimetric verification of two dimensional distributions of photon intensity by means of Beam Intensity Scanner System (BISS) as a radiation detector with a custom-made software for dose calculation of fluorescence signals from scintillator. The scintillator is used to produce fluorescence from the irradiation of 6MV photons on a Varian Clinac 21EX. The BISS reproduces 3D- relative dose distribution from the digitized fluoroscopic signals obtained by digital video camera-based scintillator(DVCS) device in the IMRT. For the intensity modulated beams (IMBs), the calculations of absorbed dose are performed in absolute beam fluence profiles which are used for calculation of the patient dose distribution. The 3D-dose profiles of the IMBs with the BISS were demonstrated by relative measurements of photon beams and shown good agreement with radiographic film. The mechanical and dosimetric properties of the collimating of dynamic and/or step MLC system alter the generated intensity. This is mostly due to leaf transmission, leaf penumbra and geometry of leaves. The variations of output according to the multileaf opening during the irradiation need to be accounted for as well. These phenomena result in a fluence distribution that can be substantially different from the initial and calculative intensity modulation and therefore, should be taken into account by the treatment planning for accurate dose calculations delivered to the target volume in IMRT.

• Proceedings of the Ginseng society Conference
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• 1984.09a
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• pp.21-26
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• 1984

The balance between metabolic activation of xenobiotics and detoxification of their active metabolites may playa vital role in controlling mutagenic and carcinogenic processes. To assess the possible role of P. ginseng C.A. Meyer in detoxification of xenobiotics, we studied the effects of ginseng on several parameters of the monooxygenasd system, including benzo(a) pyrene monooxygenase(AHH) and benzo(a) pyrene epoxide hydratase(EH) as well as effects of ginseng on the conjugation system. Test animals receiving ginseng saponin-fraction induced epoxide hydratase activity to over $150\%$ (20mg/kg b.w.) of the control and increased glutathione transferase activity (GSH-T) up to $140\%$ (20mg/kg b.w.) of the control, whereas no significant changes were observed in the benzopyrene monooxygenase activity (AHH). Such a selective induction of the inactivation enzyme epoxide hydratase, combined with a marked elevation of the detoxifying enzyme glutathione transferase, without a concurrent induction of benzopyrene monooxygenase which is responsible for the formation of carcinogenic intermediates, demonstrates that ginseng has the potential to alter the metabolic course of carcinogenic polycyclic aromatic hydrocarbons, and thereby enhance detoxification. Thus, ginseng may play an important role in the prevention of tumors caused by carcinogens.