• Fisheries and Aquatic Sciences
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• v.14 no.3
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• pp.218-225
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• 2011

Dual frequency identification sonar (DIDSON) is an imaging sonar that has been used for numerous fisheries investigations in a diverse range of freshwater and marine environments. The main purpose of DIDSON is fish counting, fish sizing, and fish behavioral studies. DIDSON records video-quality data, so processing power for handling the vast amount of data with high speed is a priority. Therefore, a semiautomated analysis of DIDSON data for fish counting, sizing, and fish behavior in Echoview (fisheries acoustic data analysis software) was accomplished using testing data collected on the Rakaia River, New Zealand. Using this data, the methods and algorithms for background noise subtraction, image smoothing, target (fish) detection, and conversion to single targets were precisely illustrated. Verification by visualization identified the resulting targets. As a result, not only fish counts but also fish sizing information such as length, thickness, perimeter, compactness, and orientation were obtained. The alpha-beta fish tracking algorithm was employed to extract the speed, change in depth, and the distributed depth relating to fish behavior. Tail-beat pattern was depicted using the maximum intensity of all beams. This methodology can be used as a template and applied to data from BlueView two-dimensional imaging sonar.

• Journal of Radiopharmaceuticals and Molecular Probes
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• v.4 no.1
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• pp.3-10
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• 2018

The folate receptor (FR) is a promising cell membrane-associated target for nuclear imaging of various cancers (via imaging $FR-{\alpha}$) and potentially also inflammatory diseases (via imaging $FR-{\beta}$), through the use of folic acid-based radioconjugates. However, there have been several drawbacks of previously reported radioconjugates, such as a short half-life of the radiolabel ($^{68}Ga\;t_{1/2}$ 68 min), a complex and time-consuming multistep radiosynthesis, and a high renal uptake of radiolabeled folate derivatives. The goal of this study was to develop an imaging probe by directly labeling folate with radioactive iodine without using an extra prosthetic group. The radiolabeling of folate was optimized using various labeling conditions and the labeled tracers were isolated by high-performance liquid chromatography. The in vitro stability of labeled folate was checked in phosphate-buffered saline and serum. The tumor-targeting efficacy of the probe was also evaluated by biodistribution studies using a murine 4T1 tumor model.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.11 no.6
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• pp.42-47
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• 2012

Followed by a paper on the Pig and Bone orthopedic prosthetic, this experiment using Phantom and Bone MRI imaging I, II of orthopedic prosthetic metal effect combines magnetic resonance imaging on metal signal-to-noise ratio(Signal to noise : SNR) and CNR(Contrast to noise: CNR), fat signal suppression(Fat-suppression) images was compared. Specimen trees to measure the reliability of the experimental reproducibility tests and statistical analysis using the SPSS statistical package was applied program SPSS(IBM SPSS Statistice 19) by * P = 0.000 < significance level $({\alpha})$ = 0.01 as a significant there was a correlation(** P < 0.01). SNR and CNR results did not directly proportional to the Titanium, Stainless, Clip CNR and fat signal suppression of the order of images of blood specimens was found to be close to the image. The impact of orthopedic prosthetic metals on magnetic resonance imaging in the diagnostic value of Titanium is relatively high and are meant more.

• Journal of Animal Reproduction and Biotechnology
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• v.39 no.2
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• pp.81-87
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• 2024

Background: Platelet-derived growth factor receptor alpha (PDGFRα) is essential for various biological processes, including fetal Leydig cell differentiation. The PDGFRα^EGFP mouse model, which expresses an eGFP fusion gene under the native Pdgfrα promoter, serves as a valuable resource for exploring PDGFRα's expression and function in vivo. This study investigates PDGFRα expression in adult testicular cells using PDGFRα^EGFP mouse model. Methods: Genotyping PCR and gel electrophoresis were used to confirm the zygosity of PDGFRα^EGFP mice. Histological examination and fluorescence imaging were used to identify PDGFRα expression within testicular tissue. Immunohistochemical analysis assessed the co-expression of PDGFRα with c-Kit, ANO-1, and TASK-1 in testicular cells. Results: Genotyping confirmed the heterozygous status of the mice, which is crucial for studies due to the embryonic lethal phenotype observed in homozygotes. Histological and fluorescence imaging revealed that PDGFRα⁺ cells were primarily located in the interstitial spaces of the testis, specifically within Leydig cells and peritubular myoid cells (PMCs). Immunohistochemical results showed PDGFRα co-localization with c-Kit and ANO-1 in Leydig cells and a complete co-localization with TASK-1 in both Leydig cells and PMCs. Conclusions: The findings demonstrate specific expression of PDGFRα in Leydig cells and PMCs in adult testicular tissue. The co-expression of PDGFRα with c-Kit, ANO-1, and TASK-1 suggests complex regulatory mechanisms, possibly influencing testicular function and broader physiological processes.

• Journal of Radiopharmaceuticals and Molecular Probes
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• v.1 no.1
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• pp.23-30
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• 2015

Radioisotopes emitting low-range highly ionizing radiation such as ${\beta}$-particles are of increasing significance in internal radiotherapy. Among the ${\beta}$-particle emitting radioisotopes, $^{67}Cu$ is an attractive radioisotope for various nuclear medicine applications due to its medium energy ${\beta}$-particle, gamma emissions, and 61.83-hour half-life, which can also be used with $^{64}Cu$ for PET imaging. The production and application of the ${\beta}$-emitting radioisotope $^{67}Cu$ for therapeutic radiopharmaceutical are outlined, and different production routes are discussed. A survey of copper chelators used for antibody labeling is provided. It has been produced via proton, alpha, neutron, and gamma irradiations followed by solvent extraction, ion exchange, electrodeposition. Clinical studies using $^{67}Cu$-labelled antibodies in lymphoma, colon carcinoma and bladder cancer patients are reviewed. Widespread use of this isotope for clinical studies and preliminary treatments has been limited by unreliable supplies, cost, and difficulty in obtaining therapeutic quantities.

• The Bulletin of The Korean Astronomical Society
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• v.39 no.2
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• pp.78.2-78.2
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• 2014

MIRIS (Multi-purpose Infrared Imaging System) is the primary payload on the Korean science and technology satellite, STSAT-3, which was launched on 2013 November 21. It is designed to observe the near-infrared sky with a $3.67^{\circ}{\times}3.67^{\circ}$ field of view and a $51.6^{{\prime}{\prime}}{\times}51.6^{{\prime}{\prime}}$ pixel resolution. Using two narrow-band filters at $1.88{\mu}m$ (Pa ${\alpha}$ line) and $1.84+1.92{\mu}m$ (Pa ${\alpha}$ dual continuum), the Paschen ${\alpha}$ Galactic plane survey has been carrying out, and the area for the Galactic longitude from $+280^{\circ}$ to $+100^{\circ}$ (with the width of $-3^{\circ}$ < b < $+3^{\circ}$) has been covered by 2014 August 31. In this contribution, we present the preliminary results of the MIRIS Paschen ${\alpha}$ emission maps and compare them with other wavelength maps such as $H{\alpha}$ and dust maps. Many of the Paschen ${\alpha}$ features have been detected along the plane, and some of them are weak or invisible in the $H{\alpha}$ map and coincide well with dense cloud regions.

• Journal of Radiopharmaceuticals and Molecular Probes
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• v.5 no.1
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• pp.36-47
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• 2019

Hypoxia-inducible factor-1 ($HIF-1{\alpha}$) is a transcription factor activated in response to low oxygen level, and is highly expressed in many solid tumors. Moreover, $HIF-1{\alpha}$ is a representative biomarker of hypoxia and also helps to maintain cell homeostasis under hypoxic condition. Most solid tumors show hypoxia, which induces poor prognosis and resistance to conventional cancer therapies. Thus, early diagnosis of hypoxia with positron emission tomography (PET) radiotracer would be highly beneficial for management of malignant solid tumors with effective cancer therapy. YC-1 is a most promising candidate among several $HIF-1{\alpha}$ inhibitors. As an effort to develop a hypoxia imaging tool as a PET radiotracer, we designed and synthesized [$^{18}F$]DFYC based on potent derivative of YC-1 and performed preliminary in vitro cell uptake study. [$^{18}F$]DFYC showed a significant accumulation in SKBR-3 cells among other cancer cells, proving as a good lead to develop a hypoxic solid tumor such as breast cancer.

• International Journal of Oral Biology
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• v.38 no.3
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• pp.87-92
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• 2013

Periodontal inflammation increases the risk of tooth loss, particularly in cases where there is an associated loss of alveolar bone and periodontal ligament (PDL). Histological and morphometric evaluation of periodontal inflammation is difficult. Especially, the lengths of the periodontal ligament and interdental alveolar bone space have not been quantified. A quantitative imaging procedure applicable to an animal model would be an important clinical study. The purpose of this study was to quantify the loss of alveolar bone and periodontal ligament by evaluation with micro-computed tomography (micro-CT). Another purpose was to investigate differences in infections with systemic E. coli LPS and TNF-${\alpha}$ on E. coli lipopolysaccharide (LPS) in loss of alveolar bone and periodontal ligament model on mice. This study showed that linear measurements of alveolar bone loss were represented with an increasing trend of the periodontal ligament length and interdental alveolar process space. The effects of systemic E. coli LPS and TNF-${\alpha}$ on an E. coli LPS-induced periodontitis mice model were investigated in this research. Loss of periodontal ligament and alveolar bone were evaluated by micro-computed tomography (micro-CT) and calculated by the two- and three dimensional microstructure morphometric parameters. Also, there was a significantly increasing trend of the interdental alveolar process space in E. coli LPS and TNF-${\alpha}$ on E. coli LPS compared to PBS. And E. coli LPS and TNF-${\alpha}$ on E. coli LPS had a slightly increasing trend of the periodontal ligament length. The increasing trend of TNF-${\alpha}$ on the LPS-induced mice model in this experiment supports the previous studies on the contribution of periodontal diseases in the pathogenesis of systemic diseases. Also, our findings offer a unique model for the study of the role of LPS-induced TNF-${\alpha}$ in systemic and chronic local inflammatory processes and inflammatory diseases. In this study, we performed rapidly quantification of the periodontal inflammatory processes and periodontal bone loss using micro-computed tomography (micro-CT) in mice.

• Journal of The Korean Astronomical Society
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• v.38 no.4
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• pp.463-470
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• 2005

In this paper, we have made the component-based development of observational software for KASI solar imaging spectrograph (KSIS) that is able to obtain three-dimensional imaging spectrograms by using a scanning mirror in front of the spectrograph slit. Since 2002, the KASI solar spectrograph has been successfully operated to observe solar spectra for a given slit region as well as to inspect the response functions of narrow band filters. To improve its capability, we have developed the KSIS that can perform sequential observations of solar spectra by simultaneously controlling the scanning mirror and the CCD camera via Visual C++. Main task of this paper is to introduce the development of the component-based software for KSIS. Each component of the software is reusable on the level of executable file instead of source code because the software was developed by using CBD (component-based development) methodology. The main advantage of such a component-based software is that key components such as image processing component and display component can be applied to other similar observational software without any modifications. Using this software, we have successfully obtained solar imaging spectra of an active region (AR 10708) including a small sunspot. Finally, we present solar $H{\alpha}$ spectra ($6562.81{\AA}$) that were obtained at an active region and a quiet region in order to confirm the validity of the developed KSIS and its software.

• The Bulletin of The Korean Astronomical Society
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• v.43 no.2
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• pp.46.1-46.1
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• 2018

The Fast Imaging Solar Spectrograph (FISS) on the Goode Solar Telescope (GST) at Big Bear Solar Observatory is the imaging Echelle spectrograph developed by the Solar Astronomy Group of Seoul National University and the Solar and Space Weather Group of Korea Astronomy and Space Science Institute. The instrument takes spectral data from a region on the Sun in two spectral bands simultaneously. The imaging is done by the organization of intensity data obtained from the fast raster scan of the slit over the field of view. Since the scan repeats many times, the whole set of data can be used to construct the movies of monochromatic intensity at arbitrary wavelengths within the spectral bands, and those of line-of-sight velocity inferred from different spectral lines. So far there are two standard observing configurations: one recording the $H{\alpha}$ line and the Ca II 8542 line simultaneously, and the other recording the Na I D2 line and Fe I 5435 line simultaneously. We have developed the procedures to produce the standard data for each observing configuration. The procedures include the spatial alignment, the correction of spectral shift of instrumental origin, and the lambdameter measurement of the line wavelength. The standard data include the movie of continuum intensity, the movies of intensity and velocity inferred from a chromospheric spectral line, the movies of intensity and velocity inferred from a photospheric line. The processed standard data will be freely available online (fiss.snu.ac.kr) to be used for research and public outreach. Moreover, the IDL procedures will be provided on request as well so that each researcher can adapt the programs for their own research.