• Proceedings of the Korean Vacuum Society Conference
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• 2015.08a
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• pp.230.1-230.1
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• 2015

One of the major problems of biological ToF-SIMS imaging is the lack of protein and peptide imaging. Most of biological story telling is mianly based on proteins. The biological implication of lipid ToF-SIMS imaging would be much higher if protein imaging is provided together. Utilizing high secondary ion yields of metals, proteins can be ToF-SIMS imaged with nanoparticle tagged proteins. Nanoparticles such as Fe3O4, SiO2, PbS were used for imaing NeuN, MCH, Orexin A, ${\alpha}$ synucline, TH(Tryosine Hydroxylase) in mouse tissues with a spatial resolution of ${\sim}2{\mu}m$ using a TOF-SIMS. Lipids and neurotransmitters images obtained simultaneously with protein images were overlayed for more deeper understanding of neurobiology, which is not allowed by any other bioimaging technqiues. The protein images from TOF-SIMS were compared with confocal fluorescence microscopy and NanoSIMS images. A new sample preparation method for imaging single cell membranes in a tissue using the vibrotome technique to prepare a tissue slice without any fixation and freeze drying will be also presented briefly for Hippocampus and Hypothalamus tissues.

• Proceedings of the KOSOMBE Conference
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• v.1993 no.11
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• pp.23-26
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• 1993

In MR functional imaging, it is shown that the signal change during photic activation is composed of two terms, i.e. the inflow effect and the susceptibility effect. Relatively the inflow effect affects the data obtained by CGE on the condition of short $T_E$(15ms) and large $\alpha$(90degree). The susceptibility effect, however, mainly contributes to the data on the condition of large $T_E$(35ms) and small $\alpha$(30degree). In this apper, we will discriminate the susceptibility effect for the intermingled data affected both flow effect and susceptibility effect. Finally susceptibility only functional imaging is proposed by using TRFGE.

• Journal of Biomedical Engineering Research
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• v.29 no.5
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• pp.355-363
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• 2008

In order to extract only the alpha activity related signals from EEG recordings, we have applied Constrained Independent Component Analysis (cICA), a new extension of ICA in which some a priori knowledge of the alpha activity is utilized to extract only desired components. Its extraction (or filtering) performance has been compared to that of the conventional band-pass filtering via the scalp alpha power maps and cortical source maps of the alpha activity. Our results demonstrate that the alpha power maps and cortical source maps from the cICA-extracted alpha signals reveal more focalized alpha generating regions of the brain than those from the band-pass filtered alpha EEG signals. Furthermore they match more closely the activated regions of the brain mapped using fMRI, validating our results. We believe that the cICA-based filtering approach of EEG signals is a more effective means of extracting a specific brain activity reflected in EEG signals that will result in more accurate source localization or imaging maps.

• Journal of Radiopharmaceuticals and Molecular Probes
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• v.1 no.1
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• pp.46-52
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• 2015

Herein we present the synthesis of $^{68}Ga$-labeled gold nanoparticles for in vivo PET imaging. A novel chelator DTPA-Cys was easily prepared from diethylenetriaminepentaacetic dianhydride in high yield. The ${\alpha}_v{\beta}_3$ integrin receptor targeted gold nanoparticle probe was synthesized by using DTPA-Cys, polyethylene glycol and cRGD peptide. $^{68}Ga$ labeling of cRGD conjugated gold nanoparticle was carried out at $40^{\circ}C$ for 30 min. Observed radiochemical yield was more than 75% as determined by radio-TLC and the probe was purified by centrifugation. In vitro stability test showed that 90% of $^{68}Ga$-labeled gold nanoparticle probe was stable in FBS for 1 h. Those results demonstrated that $^{68}Ga$-labeled gold nanoparticle could be used as a potentially useful probe for specific tumor imaging.

• The Bulletin of The Korean Astronomical Society
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• v.38 no.2
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• pp.86.2-86.2
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• 2013

Ellerman bombs(EBs) are emission features at the wings of the H alpha spectral line. They are believed to be a kind of a magnetic reconnection feature in the low chromosphere or near photosphere. It was previously reported that surges often occur in association with EBs. However, previous observations were restricted to imaging observation. Using Fast Imaging Solar Spectrograph installed in New Solar Telescope at Big Bear Solar Observatory, California, we observed 5 EBs and associated surges with high-spatial and high-spectral resolutions. In this presentation, we will show the results and discuss the physical properties.

• Publications of The Korean Astronomical Society
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• v.21 no.2
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• pp.51-59
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• 2006

We have successfully developed the KASI (Korea Astronomy and Space Science Institute) Solar Imaging Spectrograph (KSIS), which has been originally upgraded from the KASI solar spectrograph that was able to record solar spectra for a given slit region and to inspect the response function of narrow band filters. A prototype KSIS was developed in 2004 by using a scanning mirror in front of the spectrograph slit and a SBIG ST-8XE CCD camera. Its main disadvantage is that it took a long time (about 13 minutes) to scan a whole active region. In this work, we have upgraded the KSIS by installing a much faster Dalsa 1M15 CCD camera, which gives a data acquisition time of about 2.5 minutes. The software for KSIS was also improved for the new CCD camera on the basis of component-based development method. We have successfully made a test observation for a simple and small active region (AR10910) using the improved KSIS system. Our observations show that H-alpha images for several wavelengths have typical features in a sunspot as well as a H-alpha centerline image is quite similar to a BBSO H-alpha image, demonstrating the capability of the KSIS system.

• The Bulletin of The Korean Astronomical Society
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• v.38 no.1
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• pp.61.2-61.2
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• 2013

We study chromospheric oscillations including umbral flashes and running penumbral waves in a sunspot using scanning spectroscopy in H-alpha and Ca II 8542A, with the Fast Imaging Solar Spectrograph (FISS) at the 1.6 meter New Solar Telescope at Big Bear Solar Observatory. A bisector method is applied to spectral observations to construct chromospheric Doppler velocity maps. Temporal sequence analysis of these shows enhanced high-frequency oscillations inside the sunspot umbra in both lines. Their peak frequency gradually decreases outward from the umbra. The oscillation power is found to be associated with magnetic-field strength and inclination, with different relationships in different frequency bands.

• The Bulletin of The Korean Astronomical Society
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• v.38 no.2
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• pp.87.1-87.1
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• 2013

Ellerman bombs(EB) are small-scale bright features observed best in the wings of H alpha line. We used the Fast Imaging Solar Spectrograph(FISS) with the 1.6m New Solar Telescope at Big Bear Solar Observatory, in order to investigate characteristics of each EB. We analyzed H alpha line profiles of EBs, and classified EBs by their contrast profiles. To analyse characteristics of EBs, we applied power-law fitting ($C={\alpha}{\Delta}{\lambda}^{-n}$) to the EB contrast profiles. The amplitude ${\alpha}$ is a measure of the strength of an EB event (or the amount of released energy), and the power-law index n is a measure of spatial concentration of energy in the higher layers of the solar atmosphere. With the two parameters, we classified EBs into a few groups. We try to understand the physical properties of each group.

• The Bulletin of The Korean Astronomical Society
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• v.37 no.1
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• pp.87.2-87.2
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• 2012

H${\alpha}$ transient bright kernels may be an important diagnostic of energy conversion processes occurring in the choromosphere during flares. We observed an H${\alpha}$ kernel that occurred in AR 11263 in associated with a small flare on 2011 Autust 5th using the Fast Imaging Solar Spectrograph installed at the 1.6m New Solar Telescope of Big Bear Solar Observatory. We find that both the H${\alpha}$ line and the CaII 8542${\AA}$ line appear in emission, with a red asymmetry in that they display red wings of enhanced emission. The red asymmetry shows 5-30 km/s downward motion for 8 minutes. We determine some physical parameters by adopting the Cloud mode and discuss the physical meaning of these results.

• Journal of Radiopharmaceuticals and Molecular Probes
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• v.2 no.2
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• pp.73-83
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• 2016

Imaging hypoxia using positron emission tomography (PET) is of great importance for cancer therapy. [$^{18}F$] Fluoromisonidazole (FMISO) was the first PET agent used for imaging tumor hypoxia. Various radiolabeled nitroimidazole derivatives such as [$^{18}F$]fluoroerythronitroimidazole (FETNIM), [$^{18}F$]1-${\alpha}$-D-(2-deoxy-2-fluoroarabinofuranosyl)-2-nitroimidazole(FAZA), 2-(2-nitroimidazol-1-yl)-N-(3,3,3-[18F]-trifluoropropyl)acetamide ([$^{18}F$]EF-3), [$^{18}F$]2-(2-nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl) acetamide (EF-5), 3-[$^{18}F$]fluoro-2-(4-((2-nitro-1H-imidazol-1-yl)methyl)-1H-1,2,3,-triazol-1-yl)-propan-1-ol ([$^{18}F$]HX-4), and [$^{18}F$]fluoroetanidazole (FETA) were developed successively. However, these imaging agents still produce PET images with limited resolution; the lower blood flow in hypoxic tumors compared to normoxic tumors results in low uptake of the agents in hypoxic tumors. Thus, the development of better imaging agents is necessary.