• 천문학회보
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• 제36권2호
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• pp.61.2-61.2
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• 2011

Low-ionization nuclear emission-line regions (LINERs) have been generally regarded to be powered by active galactic nuclei (AGNs), yet still a number of alternative explanations on the origin of LINER emission are suggested; for example, planetary nebulae nuclei of massive stars, supernovae shocks from death of massive stars, and old stellar populations. Interestingly, a majority of recent star formation early-type galaxies (ETGs) in local universe presents such LINER emission lines. Given that situation, revealing the true nature of LINERs is a crucial step to constrain the evolution path to quiescent ETGs. To resolve the issue, we use Keck/LRIS to obtain spatially resolved spectra on a carefully selected ETG. The ETG SDSS J091628.05+420818.7 at redshift z ~ 0.024 shows modest LINER emission line features without any detection of 21 cm radio continuum nor X-ray emission. We perform a stellar continuum subtraction and measure emission line strengths and their uncertainties for each spectrum from five apertures along the slit with size of 1 arcsecond (~0.5 kpc). We find that extended spatial distributions of four emission lines $H{\alpha}$, $H{\beta}$, [OIII]${\lambda}5007$, and [NII]${\lambda}6583$, and they can be explained by central emission blurring effect. We conclude that the emissions seem to be centrally concentrated, indicating the AGN-nature of LINERs.

• 천문학회보
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• 제36권2호
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• pp.61.1-61.1
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• 2011

We have performed simultaneous observations at 22GHz and 43GHz on AGNs hosted by elliptical galaxies using KVN radio telescope. We have constructed the sample, based on two major surveys in radio and optical band, i.e. Faint Images of the Radio Sky at Twenty-Centimeters (FIRST) and Sloan Digital Sky Survey (SDSS) DR7, respectively. We restricted the redshift range 0.01 < z < 0.06 and the absolute magnitude Mr < -19.4 in order to satisfy volume limited sample. We also checked clear detection of four distinctive emission lines ([NII], [OIII], $H{\alpha}$, $H{\beta}$) so as to utilize on BPT diagram, distinguishing AGNs from star-forming galaxies. Elliptical galaxies have been selected by visual inspection making use of SDSS optical images. Then, we cross-matched the elliptical galaxies with FIRST detections. About 35% of the galaxies have been detected throughout KVN observations. We derive spectral index, applying the flux of different radio frequencies from FIRST (1.4GHz) and KVN (22GHz) and classify into steep, flat or inverted spectrum. We have found that most of the detected galaxies have flat spectrum while the rest of them have steep spectrum. This implies that a number of detected galaxies might have compact structure associated with the central region of the galaxies. The relation between black hole mass and radio luminosity has shown relatively tighter correlation in high frequency than in low frequency, which confirms that high frequency in radio band is appropriate to study the center of the galaxies.

• 대한화장품학회지
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• 제22권2호
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• pp.201-210
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• 1996

Predictive tests for the identification of contact sensitizing chemicals have been developed. We measured the sensitization potential with three predictive tests, the in vitro and the in vivo Local Lymph Node Assay(LLNA), ELISA to detect interferon-gamma(IFN-${\gamma}$) from supernatant and flow cytometry to detect change of cell surface proteins, using draining lymph nodes of mice. BALB/c mice were exposed to various chemicals or vehicles on the ears daily for 3 consecutive days in all experiments. With some exceptions of propyl paraben, neomycin sulfate, the in vivo LLNA was able to detect the sensitizing capacity of test chemicals and was more sensitive than the in vitro LLNA for chemicals used in the present study. In another experiment, contact sensitivity was assessed by the ELISA to detect IFN-Υ from the supernatants of the cultured LNCs after sensitization with chemicals. There was a good correlation between the LLNA and the IFN-Υ production for test chemicals. We also examined the change of cell surface proteins on LNCs after sensitization by flow cytometry for some cell adhesion molecules(ICAM-1, E-cadherine, B7 molecule), T cell markers(CD3, CD4, CD8, T$\alpha$$\beta$,T${\gamma}$$\delta$) and B cell markers(LR1, CD45R, I-Ad). The number of ICAM-1 positive cells and B cells in LNCs were increased after sensitization with DNCB, TNCB, isoeugenol and 25%, 50% cinnamic aldehyde compared with that of vehicle as a control. In conclusion, the in vivo LLNA could provide more sensitive screening test for moderate to strong sensitizers and some weak sensitizers including cosmetic raw materials than the in vitro LLNA. The production of IFN-Υ by allergen-activated LNCs might be a values indicators without radioisotopes for the identification of contact allergens. Detection of allergens by testing the increase of ICAM-1 positive cells and B cells in LNCs by flow cytometry might be used as a test method to detect allergens.

• Archives of Pharmacal Research
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• 제23권2호
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• pp.178-181
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• 2000

In order to study the simultaneous determination of (+)- and (-)-cetirizine in human urine we have developed a chiral separation method by HPLC. A chiral stationary phase of $\alpha$$_1$-acidglycoprotein, the AGP-CSP was used to separate the enantiomers. The pH of the phosphate buffer, as well as the content of the organic modifier in the mobile phase, markedly affected the chromatographic separation of (+)- and (-)-cetirizine. A mobile phase of 10 m㏖/1 phosphate buffer (pH 7.0)-acetonitrile (95 : 5, v/v) was used for the urine assays. Ultraviolet absorption was monitored at 230nm and roxatidine was employed as the internal standard for quantification. (+)-Cetirizine, (-)-cetirizine and the internal standard were eluted at retention times of 12, 16, and 32 mins, respectively. The detection limit for cetirizine enantiomers was 400 ng/$m\ell$ of urine. A pharmacokinetic study was conducted with the help of 5 healthy female volunteers who were administered with a single oral dose of racemic cetirizine (20 mg). The peak area ratios provided by the cetirizine enantiomers were linear(r>0.997) over a concentration range of 2.5-200 ${\mu}g/ml$. The peak of the excreted cetirizine enantiomers appeared in the urine sample during the period of 1-2 hrs following the administration of the oral dose. The excreted level of (+)-cetirizine was slightly higher than (-)-cetirizine but the difference was not statistically significant. However, this method appears to have applications for enantioselective pharmacokinetic studies of racemic drugs.

• 한국잡초학회지
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• 제15권3호
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• pp.206-213
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• 1995

Glyphosate를 토마토의 동화산물 공급부위에 처리하였을 때, 제초제결합 단백질인 QB 단백질을 Escherichia coli 내에서 ${\alpha}$-galactosidase가 발현되기 위해 lac Z 유전자의 3' 말단에 cloning된 시금치 psb A 유전자에 의해 발현되는 hybrid 단백질에 대한 항체를 형성시킨 후 이것을 이용하여 immunoblotting을 실시하였다. G1yphosate는 thylakoid 막의 Photosystem II내에 있는 D1 단백질의 붕괴에 영향을 주었다. LHC II 복합체내의 D1 단백질의 기능 이상은 glyphosate 의 다면발현적 효과였다.

• Journal of electromagnetic engineering and science
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• 제11권1호
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• pp.42-50
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• 2011

One of the important applications of THz time-domain spectroscopy (TDS) is the detection of explosive materials through identification of vibrational fingerprint spectra. Most recent THz spectroscopic measurements have been made using pellet samples, where disorder effects contribute to line broadening, which results in the merging of individual resonances into relatively broad absorption features. To address this issue, we used the technique of parallel plate waveguide (PPWG) THz-TDS to achieve sensitive characterization of three explosive materials: TNT, RDX, and HMX. The measurement method for PPWG THz-TDS used well-established ultrafast optoelectronic techniques to generate and detect sub-picosecond THz pulses. All materials were characterized as powder layers in 112 ${\mu}m$ gaps in metal PPWG. To illustrate the PPWG THz-TDS method, we described our measurement by comparing the vibrational spectra of the materials, TNT, RDX, and HMX, applied as thin powder layers to a PPWG, or in conventional sample cell form, where all materials were placed in Teflon sample cells. The thin layer mass was estimated to be about 700 ${\mu}g$, whereas the mass in the sample cell was ~100 mg. In a laboratory environment, the absorption coefficient of an explosive material is essentially based on the mass of the material, which is given as: ${\alpha}({\omega})=[ln(I_R({\omega})/I_S({\omega}))]m$. In this paper, we show spectra of 3 different explosives from 0.2 to 2.4 THz measured using the PPWG THz-TDS.

• Korean Journal of Acupuncture
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• 제27권3호
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• pp.109-118
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• 2010

Objectives : The purpose of this study is to investigate the effect of Bacillus-fermented Scutellariae Radix acupuncture solution (SB) on chemokine and growth factor production in RAW 264.7 mouse macrophages stimulated by lipopolysaccharide (LPS). Methods : Productions of chemokine and growth factor were measured by High-throughput Multiplex Bead based Assay with Bio-plex Suspension Array System based on xMAP$^{(R)}$ technology. Firstly, cell culture supernatant was obtained after treatment with LPS (1 ${\mu}g$/mL) and SB for 24 hours. Then, it was incubated with the antibody-conjugated beads for 30 minutes. Detection antibody was then added and incubated for 30 minutes. After incubated for 30 minutes, strepavidin-conjugated phycoerythrin (SAPE) was added. After another 30 minutes incubation, the level of SAPE fluorescence was analyzed in Bio-plex Suspension Array System. Results : The results of the experiment are as follows. 1. SB significantly inhibited the LPS-induced production of vascular endothelial growth factor (VEGF), interferon-inducible protein (IP)-10, and granulocyte-colony stimulating factor (G-CSF) at the concentration of 25, 50, 100, and 200 ${\mu}g$/mL in RAW 264.7 cells (P < 0.05). 2. SB significantly inhibited the LPS-induced production of Eotaxin at the concentration of 25, 100, and 200 ${\mu}g$/mL in RAW 264.7 cells (P < 0.05). 3. SB significantly inhibited the LPS-induced production of MIP-$1\alpha$ at the concentration of 25 and 100 ${\mu}g$/mL in RAW 264.7 cells (P < 0.05). Conclusions : These results suggest that SB has immuno-modulatory property related with its inhibition of VEGF, IP-10, G-CSF, and Eotaxin production in macrophages.

• 한국BIM학회 논문집
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• 제6권3호
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• pp.34-41
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• 2016

The previous studies argue that using the semantic properties of BIM objects is efficient for simulating the behaviors of autonomous, computer agents, called virtual-users, but such assumption is not proven via evidence-based research approaches. Hence, this present study aims to investigate the empirical effects of a human behavior simulation model equipped the semantics of spaces on the architecture-major students' operation and understanding of the simulation system, compared to a typical path-finding model. To achieve the aim, this study analyzed the survey and interview data, collected in the authentic design projects. The analysis indicates that (1) using a simulation model equipped the semantics of spaces helps the students' operation of the simulation, and (2) it also aids understanding the relationship between the variables of spaces and virtual-users (${\alpha}=0.74$). In addition, the qualitative data inform that the advantages of the simulation model that computes the semantics of spaces stem in the automatic behavioral changes of massive numbers of virtual-users, and efficient detection and activation on the what-if situations. The analysis also reveals that the simulation model has shortcomings in orchestrating the complex data structure between the semantics properties of spaces and virtual-users under multi-sequential scenarios. The results of this study contribute to develop a future design system combining BIM with human behavior simulation.

• 한국미생물·생명공학회지
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• 제29권1호
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• pp.50-55
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• 2001

B. subtilis cx1이 생산하는 박테리오신(BSCX1)을 부분 정제하고 특성을 규명하였다. BSCX1은 pH 안정성 실험에서 pH 2.5~9.5 구간에서도 안정되게 항균활성을 유지하였다. 각종 효소에 대한 안정성 실험에서는 protease, trypsin, proteinase K, 그리고 carboxypeptidase로 처리하였을 때는 완전히 항균활성이 사라졌고, $\alpha$-amylase, lipase, aminopeptidase는 항균활성에 영향을 미치지 못하였다. 이상의 결과로 본 항균물질이 단백질계열의 물질이며 당이나 지질 결합은 항균활성에 큰 영향을 미치지 않는 것으로 추정되었다. 그러나 열에는 불안정하여 $60^{\circ}C$이상에서는 15분 안에 완전히 그 항균활성을 상실하였으며 $50^{\circ}C$ 에서는 15분만에 역가의 50%가 실활 되었다. 이에 반하여 저온에서는 무척 안정하여 $-20^{\circ}C$와 $-70^{\circ}C$에서 수개월간 보관하여도 여전히 항균활성을 그대로 유지하였다. Tricin-SDS-PAGE를 통하여 BSCX1의 분자량은 약 9,500 dalton으로 확인되었ekl. BSCX1은 pH2.5에서 9.5에 이르는 넓은 pH 영역에서 그 활성을 유지하므로, 기존에 개발된 nisin이나 유산균 유래의 항균물질이 pH 안정성이 떨어진다는 단점을 보완할 수 있는 생물학적 식품보존제로서의 개발 가능성을 높이 시사하였다.

• IMMUNE NETWORK
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• 제11권6호
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• pp.383-389
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• 2011

Background: EY-6 is one of the newly synthesized indoledione derivatives to induce tumor cell-specific cell death. In this study, we investigated the mechanism of immunological death induced by EY-6 at mouse colon cancer cell as well as at the normal immune cell represented by dendritic cell. Methods: C57BL/6 mouse syngeneic colon cancer cell MC38 was treated with EY-6, and analyzed by MTT for viability test, flow cytometry for confirming surface expressing molecules and ELISA for detection of cytokine secretion. Normal myeloid-dendritic cell (DC) was ex vivo cultured from bone marrow hematopoietic stem cells of C57BL/6 mice with GM-CSF and IL-4 to analyze the DC uptake of dead tumor cells and to observe the effect of EY-6 on the normal DC. Results: EY-6 killed the MC38 tumor cells in a dose dependent manner (25, 50 and $100{\mu}M$) with carleticulin induction. And EY-6 induced the secretion of IFN-${\gamma}$ but not of TNF-${\alpha}$ from the MC38 tumor cells. EY-6 did not kill the ex-vivo cultured DCs at the dose killing tumor cells and did slightly but not significantly induced the DC maturation. The OVA-specific cross-presentation ability of DC was not induced by chemical treatment (both MHC II and MHC I-restricted antigen presentation). Conclusion: Data indicate that the EY-6 induced tumor cell specific and immunological cell death by modulation of tumor cell phenotype and cytokine secretion favoring induction of specific immunity eliminating tumor cells.