• Title/Summary/Keyword: Alpha 4 beta 7

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Effects of Electron-Beam Irradiation and Storage on Cholesterol Oxides Products of Beef Meat (전자선 조사와 저장기간이 우육의 콜레스테롤 산화물질에 미치는 영향)

  • Lee, Jeong-Ill;Min, Joong-Seok;Kim, Il-Suk;Park, Gu-Boo;Lee, Moo-Ha
    • Korean Journal of Food Science and Technology
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    • v.30 no.6
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    • pp.1312-1320
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    • 1998
  • Some commercial beef loins in raw state were packaged with PVDC as aerobic and vacuum condition. The other beef samples were cooked until core temperature arrived at $70^{\circ}C$ and then packaged immediately in the same way as the raw state. These samples were irradiated by electron beam (0, 1, 2 kGy), and then stored in refrigerator $(2{\sim}4^{\circ}C)$. Identity and quantity of cholesterol oxides were analysed at the 0, 7th, 14th day of storage. In the samples that were raw and packaged aerobically, $7{\alpha}-hydroxycholesterol,\;{\beta}-epoxide,\;7{\beta}-hydroxycholesterol$ and 7-ketocholesterol were detected over $0.5\;{\mu}g/g$. Cholestanetriol and${\alpha}-epoxide$ were detected at levels below $0.5\;{\mu}g/g$ during storage. In the samples that were raw and vacuum-packaged, $7{\alpha}-hydroxycholesterol$, 7-ketocholesterol and cholestanetriol were detected. In the samples that were cooked and packaged aerobically, cholestanetriol and ${\alpha}-epoxide$ were detected below $0.5\;{\mu}g/g$ during storage. $7{\alpha}-hydroxycholesterol,\;{\beta}-epoxide,\;7{\beta}-hydroxycholesterol$and 7-ketocholesterol were detected as $1.53{\sim}26.81,\;1.07{\sim}5.23,\;40.64{\sim}101.30\;and\;7.16{\sim}33.91\;{\mu}g/g$, respectively. In all results, total amounts of cholesterol oxide increased significantly as irradiation dose and storage time increased (P<0.05).

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Synthesis and Characterization of Guanidine Dinitramide Crystal (구아니딘 디나이트라아마이드 결정의 합성 및 특성 분석)

  • Kim, Wooram;Kwon, Younja;Jo, Youngmin;Park, Youngchul
    • Applied Chemistry for Engineering
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    • v.26 no.6
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    • pp.737-742
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    • 2015
  • An environmentally favorable solid oxidizer, guanidine dinitramide ($H_2C(NH_2)NH_2N(NO_2)_2$), with high purity and synthesis yield was prepared using guanidine carbonate ($NH_2C(=NH)NH_2{\cdot}1/2H_2CO_3$). Two different crystalline forms (${\alpha}$-form and ${\beta}$-form) were obtained depending on the solvent used and synthesis process. Despite of the same chemical composition, Raman-IR and TGA-DSC revealed that different structures existed between them. In particular, the thermal analysis showed the exothermic temperature of ${\alpha}$-form at $155.7^{\circ}C$ while $191.6^{\circ}C$ for ${\beta}$-form. The caloric value of ${\alpha}$-form was 536.4 J/g which was 2.5 times larger than that of ${\beta}$-form, 1310 J/g. In addition, ${\alpha}$-form was steeply decomposed with one-step variation, but ${\beta}$-form followed a two-step thermal decomposition pattern.

EEG 16 channel variations between the non-stimulation and the moxibustion stimulated subjects for CV4, CV8, and CV12 acupuncture points (중완, 신궐 및 관원 경혈의 뜸 자극과 무 자극 대상군의 16채널 뇌파 변화에 관한 연구)

  • Yoon, Dong-Eop;Song, Hong-Bok
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.14 no.12
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    • pp.2755-2760
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    • 2010
  • The moxibustion therapy of orient medical is well known. Moxibustion method is used to burning moxibustion. The variation factors of the moxibustion stimulation for body can be applied to the electronic moxibustion method. This study is to analyze and examine the electroencephalogram(EEG) variations for moxibustion stimulation. In experiment, we divided six healthy male subjects into two same sized groups : with and without stimuli. The given stimuli are the moxibustion for CV4(Kwan-Won), CV8(Shin-Guel), and CV12(Jung-wan) acupuncture points. We have analyzed the power spectrum for ${\alpha}$, ${\beta}$, ${\delta}$, ${\Theta}$ waves and the average EEG variation level and each channels variation level of EEG. The result was that the moxibustion stimulation decreased the EEG levels of parietal lobe(ch 2, 3, 6, 7) according to the somatosensory system. And the apparent different points are that mild-heat($42{\sim}44^{\circ}C$) stimulation generating ${\alpha}$-wave is increased and ${\beta}$-wave is decreased, also slight-hot($45{\sim}48^{\circ}C$) stimulation made ${\alpha}$-wave decrease and ${\beta}$-wave increase for occipital lobe(ch 4,8) on the simulation group.

A Study on Contents of Sugar and the Activities of Amylase in Enzyme Foods and Enzyme-shaped Foods (효소식품과 효소표방식품 중 아밀라아제 활성과 당 함량 조사연구)

  • Kim, Myeong-Gil;Oh, Moon-Seog;Kang, Suk-Ho;Kim, Han-Taek;Yoon, Mi-Hye
    • Journal of Food Hygiene and Safety
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    • v.30 no.4
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    • pp.359-365
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    • 2015
  • The purpose of this study was to investigate the contents of sugars and ${\alpha}$-amylase and ${\beta}$-amylase activities in 98 specimen with enzyme foods and enzyme-shaped foods (the other processed foods, beverage bases, fermented drinks, liquid teas). The ${\alpha}$-amylase activity in enzyme foods and the other processed foods were ranged 4.9~53,854.6 U/g and 2.9~1,182.7 U/g, respectively, there was a big difference in the same type. The ${\alpha}$-amylase activity of the fermented products (beverage bases, fermented drinks, liquid teas) were ranged 0.1~1.7 U/g. The average of ${\beta}$-amylase activity in enzyme foods, the other processed foods, the fermented products were found 126.0 U/g, 5.6 U/g and 10.5 U/g, respectively, enzyme-shaped foods were a lot lower than enzyme foods. Total contents of sugars were average 22.4 g/100 g in enzyme foods, 14.8 g/100 g in the other processed foods, 46.9 g/100 g in beverage bases, 41.1 g/100 g in fermented drinks, 39.5 g/100 g in liquid teas, total contents of sugars appeared high amount in the fermented products. Correlations between ${\alpha}$-amylase activity and lactose content was statistically significant in enzyme foods (r = 0.644) and it was strong in the other processed foods (r = 0.903). Correlations between ${\beta}$-amylase activity and lactose content was statistically significant in enzyme foods (r = 0.648) and it was strong in the other processed foods (r = 0.757). There was a significant relationship between ${\alpha}$-amylase and ${\beta}$-amylase activities in enzyme foods and the other processed foods (r = 0.869, r = 0.760). That is, it was found that also the proportional relationship established among the ${\alpha}$-amylase activity, ${\beta}$-amylase activity.

Effect of $\alpha-SiC $seed on microstructure and fracture toughness of pressureless-sintered $\beta-SiC$ ($\alpha-SiC $seed의 첨가가 상압소결된 $\beta-SiC$의 미세구조와 파괴인성에 미치는 영향)

  • Young-Wook Kim;Won-Joong Kim;Kyeong-Sik Cho;Heon -Jin Choi
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.7 no.1
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    • pp.18-26
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    • 1997
  • $\beta-SiC $powder with or without the addition of 1 wt% of $\alpha-SiC$ particles (seeds) was pressureless-sintered at $1950^{\circ}C$ for 0.5, 2 and 4 h using $Y_3Al_5O_{12}$ (yttrium aluminum garnet, YAG) as a sintering aid. The introduction of $\alpha-SiC$ seeds into $\beta-SiC$ accelerated :he grain growth of elongated large grains during sintering, resulting in the coarser microstructure. The fracture toughnesses of materials with $\alpha$-SiC seeds and without $\alpha-SiC$ seeds sintered for 4 h were 7.5 and 6.1 $MPa\cdot \textrm m^{1/2}$, respectively. Higher fracture toughness of the material with seeds was due to the enhanced bridging by elongated grains, resulting from coarser microstructure.

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The Anti-oxidative Compounds of Smilax riparia Leaves (Smilax riparia 잎의 항산화 성분)

  • 조은선;김정일;김호현;전인주;함인혜;황완균
    • YAKHAK HOEJI
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    • v.47 no.5
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    • pp.300-306
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    • 2003
  • Rhizoma of Smilax china has been used as anti-inflammatory and analgesic, antiedemic agent in Korean folk medicine. In order to investigate the efficacy of anti-oxidative activity, the activity-guided fractionation and the isolation were performed. Each fractions ($H_2O$ fraction, 20%, 40%, 60%, 100% MeOH fractions and CHCl$_3$ fraction) was examined antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging potential. It was revealed that 40%, 20% MeOH fractions and $H_2O$ fractions have significant anti-oxidative activity. From 40% and 20% MeOH fractions two flavonoid glycosides and one procyanidin were isolated and elucidated apigenin-7-Ο-$\alpha$-L-rhamnopyranosyl(1\longrightarrow2)-$\beta$-D-glucopyranoside, apigenin-7-Ο-$\alpha$-L-rhamnopyranosyl(1\longrightarrow6)-$\beta$-D-glucopyranoside and catechin(4$\alpha$\longrightarrow6)epicatechin through their physicochemical data and IR, FAB-MS, $^{13}$ C-NMR, and $^1$H-NMR analysis with authentics, respectively. The isolated compounds were examined by DPPH method. Apigenin-7-Ο-$\alpha$-L-rhamnopyranosyl(1\longrightarrow2)-$\beta$-D-glucopyranoside and catechin (4$\alpha$\longrightarrow6) epicatechin showed powerful radical scavenging activities on DPPH radical among three compounds.

The Relationship Between the Expression of Estrogen Receptor ${\beta}$ and Recurrence in Breast Cancer (에스트로겐 수용체 ${\beta}$ 발현과 유방암 재발과의 관련성)

  • Kang, Su-Hwan;Choi, Jung-Eun;Lee, Soo-Jung
    • Journal of Yeungnam Medical Science
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    • v.28 no.2
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    • pp.153-164
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    • 2011
  • Background: It has been reported that estrogen receptor beta ($ER{\beta}$) mRNA expression was down-regulated during carcinogenesis and was inversely related to estrogen receptor alpha ($ER{\alpha}$) expression in breast cancer. The association of $ER{\beta}$ mRNA expression to tamoxifen resistance has also been reported. In this study, the expression of $ER{\alpha}$ and $ER{\beta}$ via immunohistochemistry (IHC) and reverse transcription-polymerase chain reaction (RT-PCR) was prompted, and an attempt was made to find out the relationship between $ER{\beta}$ expression and recurrence in the hormonal therapy group, and between $ER{\beta}$ expression and known prognostic factors. Methods: Tumor specimens were obtained at surgery from 67 female breast cancer patients during the period of September 1995 to December 2000. All the specimens were frozen in liquid nitrogen and kept at $-70^{\circ}C$ until they were used. The medical records were analyzed retrospectively. The expressions of ER were analyzed using IHC and RT-PCR methods. Results: The median follow-up was at 93.0 months (range: 14-157 months). The percentage of $ER{\alpha}+/ER{\beta}+$, $ER{\alpha}+/ER{\beta}-$, $ER{\alpha}-/ER{\beta}+$, and $ER{\alpha}-/ER{\beta}$ group were 35.9% 9.4%, 47.2%, and 7.5%, respectively, in 53 patients with hormonal therapy. $ER{\beta}$ was positive in 42 (82.3%) of 51 ER-positive patients. In the hormonal therapy group, the recurrence rates of each group was 15.8%, 0%, 40.0%, and 0%, respectively. In this group, the $ER{\beta}$ expression tended to recur, but there was no clinical significance (p=0.084). Conclusion: The $ER{\beta}$ expression may be a predictive marker of a poor response to endocrine therapy in breast cancer patients, although this needs to be confirmed in additional studies.

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Stabilization of Amylolytic Enzymes by Modification with Periodate-Oxidized Soluble Starch (과요오드산 산화전분 변형에 의한 아밀라아제의 안정화)

  • ;Tri;Kazuo Ito;Masaru Iizuka;Noshi Minamiura
    • The Korean Journal of Food And Nutrition
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    • v.11 no.5
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    • pp.561-564
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    • 1998
  • The stabilizatio of amaylolytic enzyme such as $\beta$-amylase of barley, $\beta$-amylase of wheat, $\beta$-amylase of sweet potato, $\alpha$-amylase of Bacillus licheniformis, $\alpha$-amylase of Aspergillus sp. and $\alpha$-glucosidase of Aspergillus awamori was attained by modification with periodate-oxidized soluble starch. The pH stability of modified enzyme was increased at pH 9 for $\beta$-amylase of sweet potato, pH 3~5 and 8~11 for $\beta$-amylase of barley, pH 2~3 and 7~12 for $\beta$-amylase of wheat and pH 6 for $\alpha$-glucosidase of Aspergillus awamori. Thermal stability increased 17.6% for $\alpha$-amylase of Aspergillus sp. at 6$0^{\circ}C$ for 10min, 30% for $\alpha$-amylase of Bacillus licheniformis at 10$0^{\circ}C$ for 5min and 4.5% for $\alpha$-amylase of sweet potato at 6$0^{\circ}C$ for 10min compared with those of native enzymes.

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PAPER ELECTROPHORETIC SEPARATION OF SERUM PROTEINS IN CATTLE AND SWINE (여지전기영동법(濾紙電氣泳動法)에 의(依)한 한우(韓牛) 급(及) 돈(豚)의 정상혈청단백질분획(正常血淸蛋白質分劃)에 대(對)한 연구(硏究))

  • Rim, Bong Ho
    • Korean Journal of Veterinary Research
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    • v.4 no.1
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    • pp.1-6
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    • 1964
  • The ratios of cattle and swine serum proteins taken from the slaughter house were studied by Paper Electrophoresis. 1. Of 79 cattle and 53 swine, 49 cattle and 32 swine were observed in this studying as normal animals, the rest which was over 60% of albumin, globulin values and 1/2 of A/G (albumin/globulin) ratio was observed separately as abnormalities, because physiological examination was not made before slaughter. The ratios of the normal serum proteins were A (albumin) 58.8, ${\alpha}$(alpha-globulin) 13.7, ${\beta}$(beta-globulin) 11.9, ${\gamma}$(gamma-globulin) 28.6, G(total globulin) 49.2, A/G 1.03 in cattle and A 48.4, ${\alpha}$ 18.0, ${\beta}$ 13.6, ${\gamma}$ 20.0, G 51,6, A/G 0.93 in swine, the result including abnormalities showed A 45.5, ${\alpha}$ 14.8, ${\beta}$ 12.5, ${\gamma}$ 26.7, G 54.5, A/G 0.83 in cattle and A 44.5, ${\alpha}$ 19.8, ${\beta}$ 13.7, ${\gamma}$ 21.8, G 55.3, A/G 0.80 in Swine. 2. The A/G ratio of cattle and swine were 1.03 and 0.93 respectively, the A/G ratio of Korean cattle and swine are higher than the ration reported of others. Although A/G ratio of swine was below 1.00, and its value showed slightly higher than the others. The A/G ratio in this result including the abnormalities was relatively low but this ratio was higher than that values obtained by other reporters. 3. Twenty nine percent of cattles and 34 per cent of swines in this study, fluctuation of A/G ratio was great. The values of ${\alpha}$ and ${\gamma}$ globulins thought to be influenced by the amount of total globulin except ${\beta}$-globulin in swine. To obtain more occurate results, more sample size is required, in other hand some animals that is in subclinical condition might influence the values of this study. 4. The ratios of each fraction mobility which were regarded albumin as 100 were A 100, ${\alpha}$ 73, ${\beta}$ 47, ${\gamma}$ 30 in Cattle and A 100, ${\alpha}$ 71, ${\beta}$ 46, ${\gamma}$ 30 in Swine.

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Oxidation of carbohydrates and A corbon-13 n. m. r. study of the keto sugars

  • An, Seung-Ho
    • Archives of Pharmacal Research
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    • v.9 no.4
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    • pp.229-232
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    • 1986
  • Three inexpensive oxidation reagents, namely pyridinium chlorochromate, chromium trioxide-dipyridine and nicotinium dichromate were utilized for oxidation of carbohydrates in 78-92% yield. Hydration could be eliminated in the oxidation of pentopyranosides and hexopyranosides, while pentofuranosides had a tendency to be easily hydrated during the oxidation. In the carbon-13 n. m. r. study, the carbonyl function resulted from the oxidation affected on the chemical shifts of $\alpha$- and $\beta$-carbons of methyl 3. 4-O-isopropylidene-$\beta$-D-arabinopyranosid-2-ulose (8) and 1,2 : 4, 5-di-O-isopropylidene-$\beta$-D-erythro-2, 3-hexodiulo-2, 6-pyranose (10) to slightly down fields (0.7-2.6 p. p. m.) compared with the chemical shifts before oxidation. While the carbonyl groups of 1. 2-O-isopropylidene-5-O-ethyloxycarbonyl-$\alpha$-D-erythro-pentofuran-3-ulose (4) and methyl 3, 5-0-isopropylidene-$\alpha$-D-threo-pentofuranosid-2-ulose (6) pushed the $\alpha$-carbons to up fields (3, 2-18.3 p. p. m. However, the order of signals on the spectra before and after oxidation remained unaltered.

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