• Title/Summary/Keyword: Aloesin

Search Result 15, Processing Time 0.03 seconds

Determination of Aloesin in Plasma by High-Performance Liquid Chromatography as Fluorescent 9-Anthroyl Derivative

  • Kim, Kyeong-Ho;Lee, Jin-Gyun;Park, Jeong-Hill;Shin, Young-Geun;Lee, Seung-Ki;Cho, Tae-Hyung
    • Archives of Pharmacal Research
    • /
    • v.21 no.6
    • /
    • pp.651-656
    • /
    • 1998
  • A sensitive high-performance liquid chromatographic (HPLC) method for the determination of aloesin in plasma was developed. After solid-phase extraction from plasma and derivatization of aloesin and compound AD-1, which was prepared from aloesin as a internal standard, with 9-anthroylnitrile in the presence of quinuclidine, the derivatives were separated on a Inertsil ODS-3 column using acetonitrile/methanol/water (3:1:6) as a mobile phase, and detected fluorimetrically at 460nm with excitation at 360nm. The detection limit of aloesin was 3.2ng/ml in plasma (S/N=3).

  • PDF

Preformulation Study of Aloesin in Buffered Aqueous Solutions (완충 수용액 중 Aloesin의 전처방화 연구)

  • 이윤진;곽혜선;전인구
    • YAKHAK HOEJI
    • /
    • v.46 no.3
    • /
    • pp.168-173
    • /
    • 2002
  • The physicochemical properties of aloesin, which has been recently found to reduce renal toxicity induced by cis-platin, were studied including solubility, partition coefficient ( $P_{c}$ ), osmolality, and stability. The solubility of aloesin was about 500 mg/mι, and the $P_{c}$ value for n-octanol/water was 1.01 $\pm$ 0.03. The degradation of aloesin followed the pseudo-first-order kinetics and was dependent on temperature, pH and ionic strength. From the pH-rate profile, the optimal pH was found to be 2.0~3.0. Some metal ions increased the degradation rate in the rank order of M $n^{2+}$ > F $e^{3+}$ > C $u^{2+}$ > F $e^{2+}$. On the other hand, other metal ions such as B $i^{3+}$, $Ba^{2+}$, Z $n^{2+}$, N $i^{2+}$, $Co^{2+}$ and $Mg^{2+}$ did not show the unfavorable effects. After autoclaving, aloesin contents remaining were 81.8~98.8% of initial concentrations depending on pH. The most stable pH was 3.98 in the autoclaving. Osmolality increased linearly as concentration increased.sed.creased.sed.

Intestinal absorption of aloin, aloe-emodin, and aloesin; A comparative study using two in vitro absorption models

  • Park, Mi-Young;Kwon, Hoon-Jeong;Sung, Mi-Kyung
    • Nutrition Research and Practice
    • /
    • v.3 no.1
    • /
    • pp.9-14
    • /
    • 2009
  • Aloe products are one of the top selling health-functional foods in Korea, however the adequate level of intake to achieve desirable effects are not well understood. The objective of this study was to determine the intestinal uptake and metabolism of physiologically active aloe components using in vitro intestinal absorption model. The Caco-2 cell monolayer and the everted gut sac were incubated with $5-50{\mu}M$ of aloin, aloe-emodin, and aloesin. The basolateral appearance of test compounds and their glucuronosyl or sulfated forms were quantified using HPLC. The % absorption of aloin, aloe-emodin, and aloesin was ranged from 5.51% to 6.60%, 6.60% to 11.32%, and 7.61% to 13.64%, respectively. Up to 18.15%, 18.18%, and 38.86% of aloin, aloe-emodin, and aloesin, respectively, was absorbed as glucuronidated or sulfated form. These results suggest that a significant amount is transformed during absorption. The absorption rate of test compounds except aloesin was similar in two models; more aloesin was absorbed in the everted gut sac than in the Caco-2 monolayer. These results provide information to establish adequate intake level of aloe supplements to maintain effective plasma level.

Aloesin and Arbutin Inhibit Typrosinase Activity in a Synergistic Manner via a Different Action Mechanism

  • Jin, Ying-Hua;Lee, Suk-Jin;Chung, Myung-Hee;Park, Jeong-Hill;Park, Young-In;Cho, Tae-Hyeong;Lee, Seung-Ki
    • Archives of Pharmacal Research
    • /
    • v.22 no.3
    • /
    • pp.232-236
    • /
    • 1999
  • In this study, we present evidence that cotreatment of aloesin and arbutin inhibits tyrosinase activity in a synergistic manner by acting through a different action mechanism. Aloesin or arbutin similarly inhibited enzyme activity of human- and mushroom-tyrosinases with an IC50 value of 0.1 or 0.04 mM, respectively. Lineweaver-Burk plots of the enzyme kinetics data showed that aloesin inhibited tyrosinase activity noncompetitively with a Ki value of 5.3 mM, whereas arbutin did it competitively (Maeda, 1996). We then examined whether cotreatment of these agents inhibits the tyrosinase activity in a synergistic manner. The results showed that 0.01 mM aloesin in the presence of 0.03 mM arbutin inhibited activity of mushroom by 80% of the control value and the reverse was also true. The inhibitory effects were calculated to be synergistic according to the B rgi method. Taken together, we suggest that aloesin along with arbutin inhibits in synergy melanin production by combined mechanisms of noncompetitive and competitive inhibitions of tyrosinase activity.

  • PDF

Determination of Aloesin in Aloe Preparations by HPLC (고속액체크로마토그래피에 의한 알로에 제제 중의 알로에신의 정량)

  • Kim, Kyeong-Ho;Kim, Hyun-Ju;Park, Jeong-Hill;Shin, Young-Geun
    • YAKHAK HOEJI
    • /
    • v.40 no.2
    • /
    • pp.177-182
    • /
    • 1996
  • The contents of aloesin in aloe preparations were determined by HPLC. Aloesin was extracted 3 times with ethanol for 30 minutes. The ethanol extract was concentrated and suspend ed in saturated NaCl aqueous solution and successively partitioned with dichloromethane, n-butanol. Prepared samples were analyzed by HPLC on a reverse column(Inertsil ODS-2). In assay, internal standard was a puerarin and regression of calibration curve was 0.998. Recoveries of aloesin added to aloe preparation were 98~123(%).

  • PDF

Effects of aloesin on physiological changes in rats after multiple oral administration

  • Yoon, Chi-Ho;Shin, Beom-Soo;Lee, Byung-Mu;Yoo, Sun-Dong
    • Proceedings of the PSK Conference
    • /
    • 2003.04a
    • /
    • pp.191.2-192
    • /
    • 2003
  • This study was conducted to examine the effect of subchronic oral administration of aloesin on changes in the body weight and blood biochemistry in rats. Aloesin were given orally at a rate of 100 mg/kg every 12 hours for 15 days. The rats in the control group received isotonic saline. Thebody weight and food consumption were measured every 12hrs immediately prior to each treatment throughout the study period. (omitted)

  • PDF

Studies on Screening of Paraquat Toxicity Reducing Agent and its Inhibition Mechanism (Paraquat 독성 경감제 검색 및 그 억제 기전에 관한 연구)

  • Lee, Jeong-Hun;Koo, Sung-Ja;Choung, Se-Young
    • Korean Journal of Food Science and Technology
    • /
    • v.30 no.1
    • /
    • pp.192-198
    • /
    • 1998
  • In this study, we intended to evaluate the modulatory effects of natural products, ${\beta}-carotene$, aloesin and semiessential amino acid, taurine on the toxicitiy of paraquat. In the taurine treated groups, serem glutamic oxaloacetic transaminase (s-GOT), serem glutamic pyruvic transaminase (s-GPT). blood urea nitrogen (BUN), creatinine, malondialdehyde (MDA), alkaline phosphatase (ALP) activity in serum and MDA, ALP activity, collagen in lung tissue were decreased to the normal values. In the aloesin treated groups, s-GPT, BUN, creatinine, MDA level in serum were decreased to the normal values significantly. In the ${\beta}-carotene$ treated group, only s-GPTactivity was reduced to the normal values. In the lung tissue of taurine treated groups, MDA value, G-6-phosphatase activity and collagen synthesis were recovered to the normal valuse and ALP activity was increase about 40%. From these results, we concluded that taurine is an effective agent to inhibit the pulmonary and internal organs toxicities induced by paraquat and the inhibition effects of taurine are due to remove free radicals directly.

  • PDF

Neuroprotective Effect of Aloesin in a Rat Model of Focal Cerebral Ischemia

  • K.J. Jung;Lee, M.J.;E.Y. Cho;Y.S. Song;Lee, Y.H.;Park, Y.L.;Lee, Y.S.;C. Jin
    • Proceedings of the Korean Society of Applied Pharmacology
    • /
    • 2003.11a
    • /
    • pp.62-62
    • /
    • 2003
  • It is now convincing that free radical generation is involved in the pathophy siological mechanisms of ischemic stroke, particularly in ischemia-reperfusion injury. The present study, therefore, examined neuroprotective effect of aloesin isolated from Aloe vera, which was known to have antioxidative activity, in a rat model of transient focal cerebral ischemia. Transient focal cerebral ischemia was induced by occlusion of middle cerebral artery for 2 hr with a silicone-coated 4-0 nylon monofilament in male Sprague-Dawley rats under isoflurane anesthesia Aloesin (1, 3, 10, 30 and 50 mg/kg/injection) was administered intravenously 3 times at 0.5, 2 and 4 hr after onset of ischemia. Neurological score was measured 24 hr after onset of ischemia immediately before sacrifice. Seven serial coronal slices of the brain were stained with 2,3,5-triphenyltetrazolium chloride and infarct size was measured using a computerized image analyzer. Treatment with the close of 1 or 50 mg/kg did not significantly reduce infarct volume compared with the saline vehicle-treated control group. However, treatments with the closes of 3 and 10 mg/kg significantly reduced both infarct volume and edema by approximately 47% compared with the control group, producing remarkable behavioral recovery effect. Treatment with the close of 30 mg/kg also significantly reduced infarct volume to a lesser extent by approximately 33% compared with the control group, but produced similar degree of behavioral recovery effect. In addition, general pharmacological studies showed that aloesin was a quite safe compound. The results suggest that aloesin can serve as a lead chemical for the development of neuroprotective agents by providing neuroprotection against focal ischemic neuronal injury.

  • PDF

Anti-inflammatory effect in macrophages according to the mixing ratio of acemannan and aloesin (Acemannan과 aloesin의 혼합 비율에 따른 대식세포에서의 항염증 효과)

  • Hyo-Min Kim;Jeong-Hwan Kim;Dan-Hee Yoo;Se-Yeong Jeon;Hyun-Jin Kim;Seon-Gil Do;In-Chul Lee;Jung-Wook Kang
    • Food Science and Preservation
    • /
    • v.31 no.2
    • /
    • pp.315-323
    • /
    • 2024
  • This study aims to confirm the anti-inflammatory activities of acemannan and aloesin, which have been studied for various efficacies at various mixed sample ratios. The mixed samples were mixed at a ratio of 1:1 (AA-1), 1:2 (AA-2), 1:3 (AA-3), 2:1 (AA-4), and 3:1 (AA-5). Seven samples were evaluated for their cytotoxic ability on macrophages, and the results showed that all cell viability was over 90% at a concentration of 100 ㎍/mL. First, due to the NO production inhibitory activity, a better inhibitory effect was achieved when using a mixed sample rather than a single material. Afterward, the activity of inhibiting the production of PGE2, TNF-α, and IL-6 was confirmed using a mixed sample. It was confirmed that AA-2 had the best inhibitory activity on producing PGE2, TNF-α, and IL-6 rather than AA-1, AA-3, AA-4, and AA-5. For this reason, experiments were conducted using AA-2 to determine the protein expression levels of iNOS and COX-2, which are inflammation-related proteins. It was confirmed that AA-2 inhibited iNOS and COX-2 protein expression by 25.01% and 27.27%, respectively, compared to the LPS-alone treatment group. In conclusion, the mixed sample of acemannan and aloesin is judged to have anti-inflammatory activity and can potentially to be used as a functional material.

Neuroprotective Mechanisms of Aloesin against Focal Ischemic Brain Injury

  • Lee, Moon-Jung;Cho, Eun-Young;Lee, Yong-Ha;Jung, Kyung-Ja;Song, Yun-Seon;Jin, Chang-Bae
    • Proceedings of the PSK Conference
    • /
    • 2002.10a
    • /
    • pp.303.1-303.1
    • /
    • 2002
  • Recent studies have suggested that the cerebral ischemia induced the neuronal cell death by mediating multiple mechanisms with necrosis and/or apoptosis. The present study examined neuroprotective mechanism of aloesin against transient focal cerebral ischemia. Aloesin. main component of aloe possesses various biological activates such as wound healing. anti-gastric ulcer. and chemopreventive activity. Transient focal cerebral ischemia was induced by 120 min MCAO. (omitted)

  • PDF