• Title/Summary/Keyword: Alnus sp.

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Growth Stimulation of Alnus firma and Robinia pseudoacacia by Dual Inoculation with VA Mycorrhizal Fungi and Nitrogen-Fixing Bacteria and Their Synergistic Effect (VA 내생균근균(內生菌根菌)과 질소고정균(窒素固定菌)으로 이중접종(二重接種)한 사방오리나무와 아까시나무의 생장촉진(生長促進)과 접종(接種)의 상승효과(相乘效果))

  • Lee, Kyung Joon
    • Journal of Korean Society of Forest Science
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    • v.77 no.2
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    • pp.229-234
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    • 1988
  • To evaluate potential of VA mycorrhizal fungi for promoting growth of nitrogen-fixing trees and efficiency of fungal inoculation in relation to soil fertility and dual inoculation with bacteria, Alnus firma was grown for six months in pots with steam-sterilized soil after inoculation with Glomus mosseae, and Robinia pseudoacacia was grown in the fumigated field after inoculation with native Glomus sp. and Gigaspora sp. In unfertilized Alnus plants, 27% increase in dry weight(or 18% in height) was observed by Glomus inoculation, while plants inoculated with both VA mycorrhiza and actinomycete(crushed nodule inoculum) showed synergistic effect of 83% dry weight increase over uninoculated plants. In fertilized Alnus plants, mycorrhizal inoculation alone or dual inoculation with actinomycete resulted in depression of height and dry weight of plants. In case of Robinia, dual inoculation stimulated height growth by 23% (or dry weight by 25%) over the control in unfertilized field, while 13% more height growth(or 21% more dry weight) was observed in fertilized field. It is concluded that VA mycorrhizae, especially Glomus mosseae, have a potential for growth enhancement in Alnus, that synergistic effect of dual inoculation(mycorrhiza + actinomycete) exists in both Alnus and Robinia, and that responses of these plants to VA mycorrhiza are more pronounced in unfertilized soil.

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Isolation of Symbiotic Frankia Strain from the Root Nodule of Alnus hirsuta (물오리나무의 뿌리혹으로부터 Frankia 공생균주의 분이)

  • 권석윤
    • Journal of Plant Biology
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    • v.32 no.1
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    • pp.1-9
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    • 1989
  • An endophyte was isolated from the root nodule of alnus hirsuta. The isolated endophyte was identified as a Frankia sp. through morphological characteristics. Their infectivity and effectivity were confirmed by nitrogen-fixing root nodules induced on inoculated Alnus seedlings. Reisolated endophyte from the induced nodule showed identical morphological characteristics as the first isolate, showing the nodule was induced by the first isolate. Consequently, the first isolate was confirmed as a true symbiont of Almus hirsuta root nodule. The isolate was designated as a Frankia SNU 014201 strain.

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Optimization for Solid Culture of Phellinus sp. by Response Surface Methodology (반응표면방법에 의한 Phellinus sp. 고체배양의 최적화)

  • Kang, Tae-Su;Kang, An-Seok;Sohn, Hyung-Rac;Kang, Mi-Sun;Lim, Yaung-Iee;Lee, Shin-Young;Jung, Sung-Mo
    • The Korean Journal of Mycology
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    • v.26 no.2 s.85
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    • pp.265-274
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    • 1998
  • This study was carried out to obtain the basic data for an artificial cultivation of Phellinus sp.. The optimum conditions for the mycelial growth on the different sawdusts (Quercus aliena, Morns alba and Alnus japonica) substrate of an isolated Phellinus sp. were optimized by response surface methodology. The ratio of rice bran addition to sawdust and the suitable moisture content for the mycelial growth in the all sawdust media were about 30% (w/w) and $65{\sim}70%$ (w/v), respectively. The initial pHs for the mycelial growth of Quercus aliena and Morns alba were in the range of $pH\;5{\sim}6$, whereas Alnus japonica was obtained at pH 6. The optimum temperature for the mycelial growth was about $25{\sim}30^{\circ}C$, depending on the different kinds of wood substrates. From the response surface analysis, the values of independent variables of Quercus aliena at stationary points were determined to be 31.01 % (w/w) of rice bran, pH of 5.31 and 69.03% (w/v) of moisture content, and the expected value of mycelial growth was about 8.32 cm. Both the ratio of rice bran addition to sawdust $(X_1)$ and moisture content $(X_3)$ were effective to the mycelial growth. In the case of Morns alba, the ratio of rice bran addition to sawdust, initial pH and moisture content at the stationary points were 28.77% (w/w), 5.28 and 69.8 (w/v),respectively, and the expected mycelial growth of 7.60 cm was obtained. Stationary points for the mycelial growth in the sawdust media of Alnus japonica were 28.74% (w/w) of rice bran, pH of 6. 04 and 66.96% (w/v) of moisture content, and the expected values of mycelial growth was about 5.38 cm. Based on the above results, there was correlations between the mycelial growth and independent variables, and the effect of rice bran $(X_1)$ and initial pH $(X_2)$ for the mycelial growth were higher than the moisture content $(X_3)$. The optimum species of sawdust media for the my celial growth of Phellinus sp. was in the order of Quercus aliena > Morns alba > Alnus japonica.

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Species Identification of Waterlogged Archaeological Woods Excavated at Shinchang-dong Wetland Site (광주신창동저습지유적출토수침목재의수종식별 - 2005년광주 ~ 장성간도로확장공사구간내 -)

  • Park, Youngman
    • Conservation Science in Museum
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    • v.10
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    • pp.29-42
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    • 2009
  • This paper explores the types (species of trees) of 25 pieces of waterlogged wood excavated from the area between Gwangju and Jangseong during road expansion by the Gwangju National Museum. These 25 pieces of wood include nine pieces of Quercus (Lepidobalanus Cerris)sp., six pieces of Quercus (Lepidobalanus Prinus)sp., three pieces of Castanea sp., two pieces of Salix sp., one piece of Alnus sp., one piece of Prunus sp., one piece of Morus sp., one piece of Chionanthus sp., and one piece of Acanthopanax sp.

Frankia sp. strain SNU 014201의 nif-H, D, K, 유전자 클로닝

  • 권석윤;강명수;안정선
    • Korean Journal of Microbiology
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    • v.30 no.1
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    • pp.30-36
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    • 1992
  • nif (nitrogen fixation)-H.D, K genes of Frankia sp. SNU 014201. a symbiotic strain isolated from root nodule of Alnus hirsura, were found to be located in the genome on 13.5 kb of EcoRI, 18.0 kb of BamHI, 10.5 kb of BglII and 4.5 kb of KpnI fragments. Using EMBL-3 BamHI arms of bacteriophage lambda. the genomic library was constructed. from which fourteen recombinant phage nif-clones were selected. Among them, Ahnif-I2 had insert DNA of 18 kb, in which 7.9 kb of BamHl fragment contained nif-H, D, K and 3.6 kb of HindlIl/KpnI had nif-H and partial -D. Therefore, the 7.9 kb and 3.6 kb fragments were subcloned and partial restriction maps were constructed. As the results, nif-F1, D.K genes were found to be located continuously on the 6.5 kb of HindII/BamHI and 5.2 kb of SalIIBamHI fragment in the genome of Frankia sp. SNU 014201.

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Introduction to Distribution and Ecology of Sterile Conks of Inonotus obliquus

  • Lee, Min-Woong;Hur, Hyeon;Chang, Kwang-Choon;Lee, Tae-Soo;Ka, Kang-Hyeon;Jankovsky, L.
    • Mycobiology
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    • v.36 no.4
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    • pp.199-202
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    • 2008
  • Inonotus obliquus is a fungus that causes white heart rot on several broad-leaved species. This fungus forms typical charcoal-black, sterile conks (chaga) or cinder conks on infected stems of the birche (Betula spp). The dark brown pulp of the sterile conk is formed by a pure mycelial mass of fungus. Chaga are a folk remedy in Russia, reflecting the circumboreal distribution of I. obliquus in boreal forest ecosystems on Betula spp. and in meridional mountain forests on beech (Fagus spp.) in Russia, Scandinavia, Central Europe, and Eastern Europe. Distribution at lower latitudes in Western and Southern Europe, Northern America, Asia, Japan, and Korea is rare. Infected trees grow for many years without several symptoms of decline. The infection can penetrate through stem injuries with exterior sterile conks developing later. In the Czech Republic, cinder conk is found on birches inhabiting peat bogs and in mountain areas with a colder and more humid climate, although it is widespread in other broad leaved species over the Czech Republic. The most common hosts are B. pendula, B. pubescens, B. carpatica, and F. sylvatica. Less frequent hosts include Acer campestre, Acer pseudoplatanus, Alnus glutinosa, Alnus incana, Fraxinus excelsior, Quercus cerris, Q. petraea, Q. robur, Q. delachampii, and Ulmus sp.

Interpretation of the Paleoclimate Environment using Large Plant Fossil of Peatland in Pyeongtaek, Central Korea

  • Lee, Jin-Hee;Cho, Kyu-Tae
    • Korean Journal of Environment and Ecology
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    • v.29 no.1
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    • pp.81-87
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    • 2015
  • This study was carried out in the peatland for estimating the paleoenvironment based on the analysis from its fossils plant and radiocarbon at the Hwayang-ri, Hyeondeok-myeon, Pyeongtaek-city, South Korea. Variety of fossil was collected from the peatland which could discriminated with naked eye and sorted into seeds, leaves, stems from four-stage of standard sieve. During analysis for the large plant fossil within the peatland, the dead plants were largely divided into 3 fossil zones according to its peatland. In the fossil zone III (the oldest layer; 6,970~6,070 yr $C^{14}$ B.P.), the observation of leaves of hydrophyte such as Trapa sp. and stems of Alnus japonica which appeared in wetland means that the environment was influenced by the climate changes such as rainfall increasing and sea level rise. In the fossil zone II (the middle layer; 6,070~5,800 yr C14 B.P.), the occurrence of Gramineae, Cyperaceae and Fagaceae indicate that the environmental condition might be more or less dry by decreasing rainfall and drop in sea level. The fossil zone I (the recent layer; 5,800~4,540 yr $C^{14}$ B.P.) where lots of herbaceous plants, increasing of Fagaceae, decreasing of A. japonica. and distribution of Pinaceae were showed, was inferred to be repeated both dry and wet environment due to human disturbance.

Studies on Cultural and Morphological Characteristics of Isolate on Ganoderma species (영지속 균주의 배양적 특성 및 형태적 특성에 관한 연구)

  • Sung, Jae-Mo;Kim, Cheon-Hwan;Moon, Hee-Woo;Kim, Soo-Ho;Lee, Hyun-Kyung
    • The Korean Journal of Mycology
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    • v.24 no.1 s.76
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    • pp.67-80
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    • 1996
  • Eleven fruit bodies of Ganoderma sp. were collected from eight locations throughout the forest of Kangwon province and Kyunggi province in Korea. The hosts in forest were cut trunks of Quercus dentata, Q. variabilis, Prunus peria and Alnus japonica that was newly surveyed but 5 isolates were collected at the farms of Ganoderma mushroom. Most fruit bodies were formed solitarily on the cut trunks but GS-106 isolate grown in crowds on cut trunk of Alnus japonica. Optimal temperature ranges for isolates of species studied were: G. applanatum $28^{\circ}C{\sim}30^{\circ}C$, G. lucidum $28{\sim}30^{\circ}C$, G. neo-japonicum $28^{\circ}C$, and G. tsuage $26^{\circ}C$ and all the species grew slowly at the $32^{\circ}C$. Hamada medium adjusted with pH 5.4 and 6.2 is better than other media for mycelial growth. Mycelial morphological characteristics of six species were studied: G. applanatum, G. lucidum and G. neo-japonicum produced typical type of staghoru hyphae but G. oregonens and G. valeosiacum produced staghoru hyphae with a branch of grape form. Clamp connection was observed on hypha of G. applanatum, G. lucidum, G. oregonense and G. valeosiacum except G. neo-japonicum with node type. Chlamydospore was produced by G. applanatum, G. neo-japonicum. and cuticular cells were present on hyphae of G. lucidum, G. neo-japonicum, G.oregonense and G. tsugae.

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