• Journal of Life Science
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• v.9 no.2
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• pp.176-181
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• 1999

In order to understand molecular phylogenetics of silkworm (Bombyx mori), we analyzed the sequences of BmoMAR isolated from Bomhyx mori that is partial coding gene of transposase of mariner-like element(MLE). By pairwise comparing nucleotide sequences of BmoMAR with ten previously reported insect MLEs accessed in GeneBank, the average genetic distance was estimated to be 0.4840. The phylogenetics tree constructed from nine insect species except for human MLE(Hsmarl) by UPGMA method indicated that MLEs are divided into three clusters, and Drosophila mariutiana was independently subgrouped. Bombyx mori(BmoMAR) was subgrouped with microcaddishfly (Orthotrichia cristata), webworm(Atteva punctella), almond moth(Ephestia cautella), Hyalopora cecropia which we lepidoptera. Phylogenetics tree according to UPGMA principle, on the basis of informative nucleotide sequences of nine insect MLEs, indicated that Bombyx mori was more closely related to microcaddishfly(Orthotrichia cristata) and webworm (Atteva punctella) of lepidoptera. We suggest that insect MLEs are a useful key for studying molecular phylogenetics among intra species of insects.

• Korean journal of applied entomology
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• v.42 no.3
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• pp.203-209
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• 2003

Biological characteristics of the almond moth, Cadra cautella, of which eggs will be substitute diets for Orius spp. and Trichogramma spp., were investigated and a mass-rearing system for the moth was developed. At 25$^{\circ}C$, egg, larval, and pupal period was 4.2, 29.8 and 8.3 days, respectively, and adult longevity was 5.8 days for female and 4.8 days for male. Total number of eggs at 20, 25 and 30$^{\circ}C$ was 128.9,207.9 and 139.9, respectively. The moth could be successfully reared with all food substrates tested, of which rice bran (50%)+chick feed (50%) assumed to be proper for massrearing in view of cost. Eggs could be stocked at 9 C for 7 days, representing 82% hatchability. In the rearing cage (16${\times}$24${\times}$9cm) used, 1,000 eggs was better for initial level of inoculation, showing relatively high emergence rate and adult weight. Mass-rearing procedures were explained in detail.