• The Korean Journal of Ecology
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• 제11권1호
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• pp.37-53
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• 1988

The most medically important snail species of Korea is Parafossarulus manchouricus, a member of the freshwater prosobranch family Bithyniidae. The human parasite that this snail transmits is Clonorchis sinensis, the "Chinese live fluke". On the other hand, this snail has physiological characteristics that reduce the turbidity of freshwater by its filiter feeding activity. However, a few basic studies have been carried out so far. The present studies were attempted to know 1) the possibility of culturing the snails and 2) allozyme variations among 5 local populations of this bithyniid snails. As the results of the studies, P. manchouricus was able to be cultivated in the lavoratory and showed considerable allozyme variations especially in the Chongpyung and Paldaing populations out of those collected from 5 localities in Korea. It is quite suggestive that the endemicity of clonorchiasis might be related to the allozyme variability.

• 한국패류학회지
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• 제15권1호
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• pp.13-20
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• 1999

A horizontal starch gel electrophoresis for enzyme proteins extracted from three Korean species and one Chinese species of Semisulcospira was carried out in order to elucidate their genetic relationships. A total of 10 enzymes were employed in three different of buffer systems. Two loci from each enzyme of GAPDH, GOT, ICDH, IDH and PEP(VL); three loci from each of three enzymes, EST, PEP(LGG) and PGDH; and five loci from GPI were observed. Most of the loci in three pleurocerid species employed showed homozygous monomorphic banding patterns and some of them were specific as genetic markers between two different species. However, EST-2, PEP(LGG-3) and PGDH-1 loci in Korean S. libertina and PEP(LGG-3), PGM-1 and PGM-2 loci in Chinese S. libertina showed polymorphic banding patterns. Three Korean Semisulcospira species including S. libertina were more closely clustered in a dendrogram within the range of genetic identity values of 0.818-0.936, and these clusters were lineated with Chinese S. libertina at the value of 0.621.

• Animal cells and systems
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• 제5권4호
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• pp.309-312
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• 2001

Allozyme studies have been widely used to estimate genetic variation and to describe genetic structure in natural populations. In many cases, the genetic diversity of recently established populations is generally lower than that of central populations. In addition, the genetic composition of an invasive species is influenced by its History of introduction as well as its ecological characters. Ageratina altissima (L.) R. King & H. Robinson (white snakeroot) is a perennial herb native to the eastern United States and Canada, and is currently receiving much attention for its rapid invasion of the Korean forests. Starch gel electrophoresis was used to assess the genetic variability at 11 putative loci in seven introduced populations of A. altissima in Seoul. Populations of A. altissima maintained lower levels of allozyme diversity (expected heterozygosity = 0.063) than those reported for other taxa with similar ecological traits. The degree of differentiation observed among A. altissima populations was considerably low. It is suggested that the populations were recently established from only a few founders via dispersal by human activities, resulting in the loss of genetic variation.

• Asian-Australasian Journal of Animal Sciences
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• 제17권9호
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• pp.1197-1203
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• 2004

This study deals with the characterization of three populations (two hatchery and one natural) of Indian major carps Catla catla and Labeo rohita from different locations in India. The genetics of Indian major carps has been completely obscure and this is the first report on comparative allozyme variations in natural and hatchery population. The total 10 biochemical genetic markers used to measure interspecific and intraspecific level of diversity. The allele frequency data indicate different level of genetic variability in three populations. The hatchery population exhibited least polymorphism, low level of heterozygosity and genetic diversity.

• 생명과학회지
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• 제15권2호
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• pp.273-278
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• 2005

붉은토끼풀(Trifolium pratense)은 전세계적으로 분포하는 근연성 초본종이다. 붉은토끼풀은 유럽 또는 북미에서 약 60년전 한국으로 도입되었다. 전분 젤 전기영동을 사용하여 한국내 분포하는 붉은토끼풀의 유전적 변이와 집단구조를 분석하기 위해 사용되었다. 붉은토끼풀에서 높은 유전적 다양성이 발견되었다. 19개 대립유전좌좌위중 10개$(52.6\%)$는 다형현상을 나타내었다. 유전적 다양성은 0.220이었다. 유성생식, 높은 다산성, 집단형성화 과정이 높은 유전적 다양성을 유지하는데 기여하는 것으로 제시하였다. 한국의 붉은토끼풀의 다양도(0.220)는 북미의 붉은토끼풀 다양도(0.285)보다 낮았다. 이는 북미의 일부 집단에서 유입되었거나 창시자효과 때문일 것으로 사료된다. 간접적 평가를 통한 세대당 이주하는 수는 4.20이였고, 유전자 유동은 한국집단에서 높게 유지되고 있음을 시사한다.

• Journal of Ginseng Research
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• 제28권1호
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• pp.60-66
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• 2004

“The Regional complex long-term program of restoration (reintroduction) of Primoryes ginseng population up to 2005” elaborated by Primorye governor administration, Regional Committee of Natural Resources and Russian Academy of Sciences operates in Russian Primorye. The Institute of Biology and Soil Science (IBSS) provides the scientific implementation of the program including the genetic analysis of extant ginseng populations, plant reproduction and off-spring identification. According to our investigations, the genetic resource of P. ginseng in Primorye is represented by three populations of wild-growing ginseng and a few pritate plantations. The results obtained by RAPD allowed concluding that the resource is dispersed among the wild and cultivated ginseng sub-populations in such a way that each of sub-populations studied has to be represented as a stock material to maintain species genetic variability. The allozyme analyses also showed that the small sub-populations of wild ginseng are characterized by unique genetic diversity and, therefore, they all need to be represented in reintroduction centers. Additionally the allozyme analysis discovered that the Blue Mountain and Khasan populations possess the most genetic diversity. So, at least one more reproductive ginseng unit has to be created besides two already existing reintroduction centers representing the Sikhote-Alin and the Blue Mountain populations.

• 한국패류학회지
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• 제15권1호
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• pp.63-69
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• 1999

가리비류 4종 - 큰가리비(Patinopecten yessoenensis), 주문진가리비(Chlamys swifti), 비단가리비(Chlamys ferreri ferreri), 해가리비(Amusium japonicum japonicum) -을 한국 5개 지역에서 채집하였고 중국산 비단가리비를 시장에서 구입하여 실험에 사용하였다. 총 7개 동위요소에 대한 starch gel 전기영동을 실시한 결과, 8개 유전자가 관찰되었다. 유전적 유사도는 비단가리비 3집단이 가장 가까운 관계를 보였고, 주문진가리비와 큰가리바가 유전적으로 서로 가까운 그룹으로 분류되었다. 이 그룹과 비단가리비가 0.595의 유사도를 보였으며 해가리비가 나머지 3종과는 유사도가 0.541로 가장 멀었다.

• Animal cells and systems
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• 제4권1호
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• pp.39-44
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• 2000

To assess the genetic diversity and population structure of Korean K. borealis, allozyme analysis was performed. The average genetic variability of Korean K. borealis populations was %P=13.2, Ho=0.048, and He=0.045. This value was the lowest in comparison with other Korean amphibian species studied. Also, the value was much lower than that of a reference population from Chinese K. borealis (%P=50, Ho=0.125, He=0.172). Wright's F-statistics showed that Korean K. borealis has distinctly low level of gene flow among regional populations (F$_{ST}$=0.339, Nm=0.487) in comparison with other Korean amphibian species studied. However, the average level of genetic divergence among Korean K. borealis populations was moderate (Nei's D=0.020). Therefore, it appeared that low levels of genetic diversity (He=0.045) and gene flow (Nm=0.487) among regional populations ave probably due to the results of decreasing population size and patchy distribution of this species in Korea.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 2002년도 학술대회지
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• pp.509-521
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• 2002

'The Regional complex long-term program of restoration (reintroduction) of Primoryes ginseng population up to 2005' elaborated by Primorye governor administration, Regional Committee of Natural Resources and Russian Academy of Sciences operates in Russian Primorye. The Institute of Biology and Soil Science (IBSS) provides the scientific implementation of this program including the genetic analysis of extant ginseng populations, plant reproduction and offspring identification. According to our investigations, the genetic resource of P. ginseng in Primorye is represented by three populations of wild-growing ginseng and a few private plantations. The results obtained by RAPD allowed concluding that this resource is dispersed among the wild and cultivated ginseng sub-populations in such a way that each of sub-populations studied has to be represented in living plant collection as a stock material to maintain species genetic variability. The allozyme analyses also showed that the small sub-populations of natural ginseng are characterized by unique genetic diversity and, therefore, they all need to be represented in reintroduction centers. Additionally the allozyme analysis discovered that the Blue Mountain and Khasan populations possess the most genetic diversity. So, at least one more reproductive ginseng unit has to be created besides two already existing reintroduction centers representing the Sikhote-Alin and the Blue Mountain populations.

• Journal of Life Science
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• 제10권1호
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• pp.14-19
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• 2000

Variation at 25 allozyme loci in Korean wateralder (Alnus hirsuta Rupr.) from nine distinct populations was measured to estimate the amount and pattern of genetic diversity and population structure. The mean genetic diversity within population was 0.166. Korean alder populations have slightly high levels of genetic diversity compared to those present in associated temperature-zone species and two Canadian alder species. Among population s genetic differentiation accounted for an significant 9% of the total variation. High gene flow(Nm=2.63) was observed. Analysis of fixation indices, calculated for all polymorphic loci in each population, showed a substantial deficiency of heterozygotes relative to Hardy-Weinberg expectations. The mean GST value A. hirsuta in Korea (GST = 0.087) is similar to those of A. rogosa in Canada (GST = 0.052). These low values of GST in two countries. reflecting little spatial genetic differentiation, may indicate extensive gene flow (via pollen and/or seeds) and/or recent colonization. These factors reduce the effect of geographic isolation of breeding and the chance for genetic divergence. A pattern of increasing is observed with increasing rainfall per year. Regression analysis indicates that 54% (F = 4.67) of the variability observed can be explained by this relationship.