• Title/Summary/Keyword: Allozyme diversity

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Allozyme Variation and Population Genetic Structure of an Invasive Plant, Ageratina altissima(White Snakeroot), in Seoul

  • Chun, Young-Jin;Lee, Hyun-Woo;Lee, Eun-Ju
    • Animal cells and systems
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    • v.5 no.4
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    • pp.309-312
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    • 2001
  • Allozyme studies have been widely used to estimate genetic variation and to describe genetic structure in natural populations. In many cases, the genetic diversity of recently established populations is generally lower than that of central populations. In addition, the genetic composition of an invasive species is influenced by its History of introduction as well as its ecological characters. Ageratina altissima (L.) R. King & H. Robinson (white snakeroot) is a perennial herb native to the eastern United States and Canada, and is currently receiving much attention for its rapid invasion of the Korean forests. Starch gel electrophoresis was used to assess the genetic variability at 11 putative loci in seven introduced populations of A. altissima in Seoul. Populations of A. altissima maintained lower levels of allozyme diversity (expected heterozygosity = 0.063) than those reported for other taxa with similar ecological traits. The degree of differentiation observed among A. altissima populations was considerably low. It is suggested that the populations were recently established from only a few founders via dispersal by human activities, resulting in the loss of genetic variation.

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Genetic Studies of Oenothera odorata Populations in Korea Based on Isozyme Analysis

  • Huh, Hong-Wook
    • Journal of Plant Biology
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    • v.39 no.3
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    • pp.223-229
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    • 1996
  • The genetic variation in Korean evening primorse (Oeothera odorata L.) populations was examiend to estimate the level of allozyme variation within populatons using starch gel electrophoresis. 7 of 13 loci (Adh, Est-1, Est-2, Mdh-2, Pgd-2, Pgm-1, and Idh) revealed (Ps=43.2%) were polymorphic. The mean number of alleles per locus (A) and polymorphic locus (Ap) for populations were 1.64 and 2.46, respectively. The effective number of alleles (Aep) within populations relatively was low ranging from 1.08 to 1.22 with a mean of 1.14. Within populations, the mean number of allele per polymorphic loci (Ap) was 2.46, the mean number of alleles per locus (A) was 1.64, and the mean genetic diversity was 0.093. About 2.7% of the total allozyme diversity resided among populations (Mean GST=0.0274). FIS, a measure of the deviation from random mating within 13 populations, was relative low (mean FIS=0.03636). The indirect estimate of gene flow, based on the mean GST, was high (Nm=8.88). Estimates of gene flow were consistent with low levels of genetic differentiation among populations.

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Comparative Genetic Diversity in Natural and Hatchery Populations of Indian Major Carps (C. catla and L. rohita)

  • Rana, R.S.;Bhat, K.V.;Lakhanpal, S.;Lakra, W.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.9
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    • pp.1197-1203
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    • 2004
  • This study deals with the characterization of three populations (two hatchery and one natural) of Indian major carps Catla catla and Labeo rohita from different locations in India. The genetics of Indian major carps has been completely obscure and this is the first report on comparative allozyme variations in natural and hatchery population. The total 10 biochemical genetic markers used to measure interspecific and intraspecific level of diversity. The allele frequency data indicate different level of genetic variability in three populations. The hatchery population exhibited least polymorphism, low level of heterozygosity and genetic diversity.

Genetic Diversity and Population Structure of Potentilla freyniana in Korea (한국내 세잎양지꽃의 유전적 다양성과 집단구조)

  • Huh, Man-Kyu
    • Journal of Life Science
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    • v.17 no.7 s.87
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    • pp.877-881
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    • 2007
  • The genetic diversity and population structure of Potentilla freyniana in Korea were determined using genetic variations at 19 allozyme loci. Thirteen of the 19 loci (68.4%) showed detectable polymorphism. Genetic diversity at the population level was high ($H_{EP}$ = 0.270). Total genetic diversity values ($H_T$) varied between 0.190 and 0.584, giving an average overall polymorphic loci of 0.371. The interlocus variation of genetic diversity within populations ($H_S$) was high (0.354). On a per locus basis, the proportion of total genetic variation due to differences among populations ($G_{ST}$) ranged from 0.008 for Fe-2 to 0.310 for Gpi with a mean of 0.065, indicating that about 6.5% of the total allozyme variation was among populations. Wide geographic ranges, perennial herbaceous nature and the persistence of multiple generations are associated with the high level of genetic variation in P. freyniana. The estimate of gene flow based on $G_{ST}$, was high among Korean populations of P. freyniana (Nm =3.57). Although P. freyniana usually propagated by asexually-produced ramets, I could not rule out the possibility that sexual reproduction occurred at a low rate because each ramet may produce terminal flowers.

Allozyme Variation of Pinus rigida Mill. in an F1-Hybrid Seed Orchard and Estimation of the Proportion of F1-Hybrid Seeds by Allozyme Analysis (잡종(雜種) 채종원(採種園)에서 리기다소나무의 Allozyme 변이(變異)와 Allozyme 분석(分析)에 의(依)한 잡종종자(雜種種字) 발생률(發生率)의 추정(推定))

  • Chung, Min Sup
    • Journal of Korean Society of Forest Science
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    • v.66 no.1
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    • pp.109-117
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    • 1984
  • Allozyme study for open pollinated seeds of forty nine pitch pine families in an $F_1$-hybrid seed orchard demonstrated that allozyme variants in aspartate aminotransferase(AAT), glutamate dehydrogenase(GDH) and leucine aminopeptidase(LAP) systems are encoded by at Least eight loci; five for AAT, one for GDH and two fur LAP. Allozyme variations showed polymorphisms at seven of the eight loci, except GDH. Average number of alleles examined over six loci were 2.33 and 2.67 for maternal and progeny groups, respectively. Average heterozygosity and genetic diversity computed over six loci were, respectively, 0.235 and 5.409 for maternal tree group, 0.238 and 5.569 for progeny group. The proportion of $F_1$-hybrid seeds estimated by allozyme analysis was 0.77%. The estimated proportion of $F_1$ hybrid seeds by allozyme study is in good agreement with the value 0.73% estimated by morphological study for the proportion of pitch ${\times}$ Loblolly $F_1$ hybrid seedlings at a nursery. Indications for Wahlund effect, high levels of self-fertilization and for non-random matings in the $F_1$ hybrid seed orchard call for cautions in estimating allele frequency changes and mating probabilities for the parental and progeny groups.

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Population Genetic Structure of Potentilla discolor Bunge, Rosaceae in Korea (한국내 솜양지꽃의 집단 유전 구조)

  • Huh, Man-Kyu
    • Journal of Life Science
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    • v.16 no.6
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    • pp.898-903
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    • 2006
  • The genetic diversity and population structure of fifteen Potentilla discolor Bunge populations in Korea were determined using genetic variations at 19 allozyme loci. Fourteen of the 19 loci (73.7%) showed detectable polymorphism. Genetic diversity at the species level and at the population level was high ($H_{ES}\;=\;0.215$, $H_{EP}\;=\;0.196$, respectively), whereas the extent of the population divergence was relatively low $(G_{ST}\;=\;0.069)$. Total genetic diversity values $(H_T)$ varied between 0.0 and 0.656, giving an average overall polymorphic loci of 0.292. The interlocus variation of genetic diversity within populations $(H_S)$ was high (0.274). On a per locus basis, the proportion of total genetic variation due to differences among populations $(G_{ST})$ ranged from 0.010 for Pgm-2 to 0.261 for Pgd-2 with a mean of 0.069, indicating that about 6.9% of the total allozyme variation was among populations. Wide geographic ranges, perennial herbaceous nature and the persistence of multiple generations are associated with the high level of genetic variation in P. discolor. The estimate of gene flow based on $G_{ST}$ was high among Korean populations of P. discolor (Nm = 3.36).

Phylogenetic Study of Two Problematic Subgenera of Tomoceridae (Insecta : Collembola) from Korea (한국산 가시톡토기 과 (곤충 강: 톡토기 목)의 문제 2아속의 계통분화)

  • 박경화;이병훈
    • Animal Systematics, Evolution and Diversity
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    • v.15 no.1
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    • pp.11-25
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    • 1999
  • Two problematic subgenera of Tomoceridae (Collembola, Insecta) were investigated for their phylogenetic relationships based on morphological characters and allozyme study from three relevant species. Different dendrograms were obtained obtained between morphological and allozyme studies. The morphological data did not give rise to any result distinctive enough to separate the two subgenera whereas the allozyme analysis produced a clear separation by the high genetic distance value. They were consistent, however, whether given rise to by using distance or cladistic methods and also whether character weighting or unweighting approaches employed in the morphological character analysis. As a consequence, it is strongly suggested that any prominent morphological trait might work as a good taxonomical character when supported by a strong genetic divergence as evidenced by allozyme analysis for instance.

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Genetic Diversity and Population Structure of Kaloula borealis (Anura, Microhylidae) in Korea

  • Yang, Suh-Yung;Kim, Jong-Bum;Min, Mi-Sook;Suh, Jae-Hwa;Kang, Young-Jin
    • Animal cells and systems
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    • v.4 no.1
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    • pp.39-44
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    • 2000
  • To assess the genetic diversity and population structure of Korean K. borealis, allozyme analysis was performed. The average genetic variability of Korean K. borealis populations was %P=13.2, Ho=0.048, and He=0.045. This value was the lowest in comparison with other Korean amphibian species studied. Also, the value was much lower than that of a reference population from Chinese K. borealis (%P=50, Ho=0.125, He=0.172). Wright's F-statistics showed that Korean K. borealis has distinctly low level of gene flow among regional populations (F$_{ST}$=0.339, Nm=0.487) in comparison with other Korean amphibian species studied. However, the average level of genetic divergence among Korean K. borealis populations was moderate (Nei's D=0.020). Therefore, it appeared that low levels of genetic diversity (He=0.045) and gene flow (Nm=0.487) among regional populations ave probably due to the results of decreasing population size and patchy distribution of this species in Korea.

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