• Journal of Plant Biology
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• 제32권3호
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• pp.173-180
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• 1989

Giemsa C-banded karyotypes of A. thunbergii, A. deltoide-fistulosum, A. cyaneum and A. cyaneum var. deltoides were analyzed, and the interspecific relationships were investigated on the basis of the C-banding patterns. The banding pattern of each species was unique and made possible an easy and clear separation among them. A. thunbergii and A. deltoide-fistulosum revealed very close banding relationship but a. cyaneum and A. cyaneum var. deltoides showed significant difference in banding pattern in spite of their close plant systematic position.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2003년도 춘계 학술대회지
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• pp.71-71
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• 2003

• 한국식품영양과학회지
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• 제43권6호
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• pp.822-828
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• 2014

본 연구에서는 3T3-L1 지방전구세포를 이용하여 Allium 속 식물의 하나인 참산부추(ASM) 메탄올 추출물의 ROS 생성 저해 및 지질 축적 억제 효과를 확인하고자 하였다. 먼저 ASM 메탄올 추출물 $100{\sim}2,000{\mu}g/mL$의 모든 농도에서 유의적인 DPPH 라디칼 소거능을 나타내었으며, 지방전구세포에 ASM 메탄올 추출물 $10{\sim}100{\mu}g/mL$를 처리하였을 때 세포 독성을 나타내지 않았다. 지방세포 내 ROS 관련 효소와 분화 관련 전사인자의 조절로 인한 중성지방 축적 저해 활성을 확인하기 위하여 지방전구세포를 분화 유도하면서 추출물을 농도별(10, 50, $100{\mu}g/mL$)로 처리하였다. 그 결과 ASM 메탄올 추출물은 대조군에 비해 ROS 생성량과 ROS 관련 효소인 G6PDH mRNA 발현을 농도 의존적으로 감소시켰다. 또한 ASM 메탄올 추출물에 의하여 지방세포 내 중성지방 축적량이 유의적으로 감소하였으며, 지방세포 분화에 관련된 전사인자인 SREBP1c, $PPAR{\gamma}$ 및 $C/EBP{\alpha}$ mRNA 발현도 유의적으로 감소하였다. 이상의 결과들로 볼 때 ASM 메탄올 추출물로 인한 ROS 생성 저해와 지질 축적 억제는 ROS 생성 및 ROS 관련 유전자의 발현감소로 인한 지방 생성 주요 전사인자의 유전자 발현 억제로 인한 것으로 보이며, ASM이 항비만 효과가 있는 천연물 소재로 개발 가능성이 있을 것으로 사료된다.

• 생약학회지
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• 제46권3호
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• pp.234-242
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• 2015

Allium hookeri (Liliaceae), native to India is widely cultivated in Korea lately as a medicinal plant. This study was performed to evaluate the antioxidant and lifespan extending effects of the root of A. hookeri. Methanol extract from the root of A. hookeri was successively partitioned as methylene chloride, ethyl acetate, n-butanol and H₂O soluble fractions. Antioxidant effects of the fractions were measured by measuring the radical scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH), and superoxide quenching activities. Lifespan extending effects of the fractions were investigated using Caenorhabditis elegans model system. In addition, stress resistant, superoxide dismutase (SOD) and catalase activities, and intracellular reactive oxygen species (ROS) levels were also studied. Furthermore, we had investigated aging-related factors such as reproduction, food intake, growth and movement of C. elegans to find any sample-induced significant change. Our results revealed that ethyl acetate soluble fraction from the root of A. hookeri showed the most potent antioxidant and lifespan extending effects, and this fraction elevated the most potent SOD and catalase activities of worms, and reduce intracellular ROS accumulation.

• 한국조경학회지
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• 제31권1호
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• pp.101-107
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• 2003

Focusing on native plants that have a high possibility of being introduced as extensive rooftop material, this study was conducted to realize extensive and easy-to-manage rooftop gardens and to raise the utilization of native plants by verifying their growing response to soil media and depth. Its result is as follows: 1) In the case of Chrysanthemum zawadskii, the top growth was better in sandy loam than in P$_1$V$_1$P$_2$, and P$_1$V$_1$P$_3$, but the mortality rate was high, making it unsuitable soil. Regarding soil depth the mortality rate was lower in 10cm than in 5cm, and it grew well in 10cm. When using it for rooftop gardens, it would be desirable to keep the minimum viable soil depth over loom. 2) In the case of Sedium middendorffianum the mortality rate was 0% regardless of soil media and depth making it very suitable material for rooftop garden. Although the flowering rate was somewhat lower in P$_1$V$_1$P$_2$, and P$_1$V$_1$P$_3$ than in sandy loam, the mortality rate was low and the root growth was good. Therefore, provided that fertilizing is managed well, it is a plant that can be highly utilized. 3) In the case of Allium senescens, the mortality rate was 0% regardless of soil or soil depth, making it a very suitable plant for extensive rooftop gardens. Although top growth was poorer in P$_1$V$_1$P$_2$, and P$_1$V$_1$P$_3$than in sandy loam the root growth was good. Therefore, provided that fertilizing is managed well, it is a plant that can be highly utilized. In conclusion the study revealed that suitable species for extensive rooftop gardens are Sedium middendorffianum and Allium senescens. However, Chrysanthemum zawadskii can be utilized greatly when fertilizing is managed regularly in artificial mixed soil over l0cm.

• 대한본초학회지
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• 제32권1호
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• pp.41-45
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• 2017

Objectives : The purpose of this study was to assume the origin and daily dose of Allii Chinensis Bulbus in Treatise on Cold Damage Diseases and Prescriptions of the Gold Chamber. Methods : I assumed the origin with Book of Materica Medica such as Illustration and Classic of Materia Medica(本草圖經), Illustration and Consideration of Plant's Name and Reality(植物名實圖考), The Korean Herbal Pharmacopoeia Forth Edition, Annotation of Hangeul Treatise of Cold Damages Diseases, and daily dose based on general principle applied on dose of Treatise on Cold Damage Diseases. Results : In Treatise on Cold Damage Diseases and Prescriptions of the Gold Chamber, the original species of Allii Chinensis Bulbus was Allium chinense G. Don. It was to be measured with weight. It was too big to write on size due to it's 10~30mm length and 3~12mm in diameter. And due to irregular size, it can't be written on number, too. Daily dose of Allii Chinensis Bulbus in Whaeyeok-san(回逆散), Gwalluhaebaekbanha-tang(栝樓薤白半夏湯) was 3 Ryang(兩), which was the most common case in Treatise on Cold Damage Diseases. And that in Gwalluhaebaekgyeji-tang(栝樓薤白桂枝湯), Gwalluhaebaekbaekju-tang(栝樓薤白白酒湯) is 8 Ryang(兩), which was the largest dose as dried herb in Treatise on Cold Damage Diseases Conclusions : The original species of Allii Chinensis Bulbus was Allium chinense G. Don. The daily dose of Allii Chinensis Bulbus was 3 Ryang(兩)(19.5g) in Whaeyeok-san and Gwalluhaebaekbanha-tang, and was 8 Ryang(兩)(52g) in Gwalluhaebaekgyeji-tang and Gwalluhaebaekbaekju-tang.

• 식품저장과 가공산업
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• 제7권1호
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• pp.9-18
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• 2008

The health benefits of garlic (Allium sativum L.) are derived from a wide variety of components and from the different ways it is administered. The known health benefits of garlic include cardiovascular protective effects, stimulation of immune function, reduction of blood glucose level, protection against microbial, viral and fungal infections, as well as anticancer effects. In the present study, it was examined the effects of water extract of A. sativum (WEAS) on the growth of cultured human tumor cells in order to investigate its anti-proliferative mechanism. Treatment of WEAS to tumor cells resulted in the growth inhibition, especially in leukemia cells, which was associated with induction of G2/M arrest of the cell cycle and apoptosis. In order to further explore the critical events leading to apoptosis in WEAS-treated U937 human leukemia cells, the following effects of WEAS on components of the mitochondrial apoptotic pathway were examined: generation of reactive oxygen species (ROS), alteration of the mitochondrial membrane potential (MMP), and the expression changes of Bcl-2 and IAP family proteins. The cytotoxic effect of WEAS was mediated by its induction of apoptosis as characterized by the occurrence of DNA ladders, apoptotic bodies and chromosome condensation in U937 cells. The WEAS-induced apoptosis in U937 cells was correlated with the generation of intracellular ROS, collapse of MMP, activation of caspase-3 and down-regulation of anti-apoptotic proteins. The quenching of ROS generation with antioxidant N-acetyl-L-cysteine conferred significant protection against WEAS-elicited ROS generation, caspase-3 activation, G2/M arrest and apoptosis. In conclusion, the present study reveals that the cellular ROS generation plays a pivotal role in the initiation of WEAS-triggered apoptotic death in U937 cells.

• 한국균학회지
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• 제20권4호
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• pp.302-308
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• 1992

Seven genera and 15 species of fungi were isolated from 50 samples of then different steps of dried onion (5 samples of each step) collected from an onion factory in Sohag Governorate, Egypt, and grown on glucose-Czapek's agar (7 genera and 15 species) and 10% NaCl glucose-Czapek's (2 genera and 6 species). The average total counts of fungi were gradually decreased throughout the different steps of drying from 2090 to zero and 152 to zero colonies/g on glucose-Czapek's agar and 10% NaCl glucose-Czapek's agar media, respectively. Aspergillus was the most common genus on the two types of media used. The dominant species were Aspergillus niger, A. flavus, A. terreus, Penicillium chrysogenum and Fusarium of oxysporum on glucose-Czapek's agar and A. terreus and A. niger on 10% NaCl glucose-Czapek's agar. The chloroform extracts of different samples were tested for the presence of mycotoxins using thin layer chromatographic analysis. The results indicated that aflatoxin was present at concentrations decreased throughout the different steps of the drying from step No. 1, onion bulbs, $120\;{\mu}g/kg$; to step No. 8, standard A, $20\;{\mu}g/kg$ while step Nos. 9 & 10 (completely dry powdered onion) were free from aflatoxin. Citrinin was also present in the first three steps at concentrations gradually decreased from 30 to 10 mg/kg.

• The Plant Pathology Journal
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• 제23권4호
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• pp.266-271
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• 2007

The genomes of different allexiviruses were isolated and cloned from virus-infected garlic plants (Allium sativum), which were collected from farm fields in the southern provinces in Korea. The partial nucleotide sequences of the genomes from different allexiviruses were clearly identified in the virus-infected garlic plants. The cloned partial genomes of viruses in garlic plants showed a greater than 90% homology to previously identified allexiviruses and classified into species of GarV-A, -B, -C, -D, -E, and -X, demonstrating that species of allexivirus found in the other countries in the world are also widely distributed in the garlic plants in Korea.

• 생명과학회지
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• 제17권12호
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• pp.1709-1716
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• 2007

본 연구에서는 몇 가지 암세포를 대상으로 마늘 열수추출물(WEAS)의 항암활성을 조사하였으며, 비교적 높은 감수성을 보인 백혈병세포를 대상으로 세포증식억제가 apoptosis 유도와 연관성이 있음을 제시하였다. WEAS에 의한 U937 세포의 apoptosis 유도는 세포주기 G2/M arrest와 연관성이 있었으며, 다양한 apoptosis조절 유전자들의 발현 변화를 동반하였음을 확인하였다. 이러한 apoptosis조절 인자들의 발현변화는 미토콘드리아 막 전위의 소실과 연관성이 있었으며, WEAS에 의한 암세포의 G2/M arrest에 의한 apoptosis 유발에 ROS가 중요한 조절자로 작용함을 알 수 있었다.