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Multiplex Real-Time Polymerase Chain Reaction Analysis of Pathogens in Peri-Implantitis and Periodontitis: A Randomized Trial

  • Eun-Deok Jo
    • Journal of dental hygiene science
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    • v.24 no.2
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    • pp.97-106
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    • 2024
  • Background: Periodontitis and peri-implantitis are diseases caused by pathogenic microorganisms that cause tissue damage and alveolar bone destruction resulting in the loss of teeth and implants. Due to the biological differences in the tissues surrounding the implants, peri-implantitis progresses more rapidly and intensely than periodontitis, underscoring the importance of understanding the characteristics and interactions of pathogenic bacteria. This study aimed to quantitatively analyze the pathogenic microorganisms associated with periodontitis and peri-implantitis in Korean patients and evaluate the correlation between these bacteria. Methods: A total of 98 (52 males and 46 females) were randomly selected and classified into three groups (healthy group [HG]=25; periodontitis group [PG]=31; and peri-implantitis group [PIG]=42). The relative expression levels of 11 pathogenic microorganisms collected from the gingival sulcus fluid were determined using multiplex real-time polymerase chain reaction. Results: Eikenella corrodens, Fusobacterium nucleatum, and Prevotella nigrescens were highly prevalent in the HG, PG, and PIG patients. The results of the relative quantitative analysis of microorganisms showed that all bacteria belonging to the green, orange, and red complexes were significantly more abundant in the PG and PIG than in the HG (p<0.05). Porphyromonas gingivalis in the red complex showed a positive correlation with all microorganisms in the orange complex (p<0.05). Campylobacter rectus in the orange complex showed a significant positive correlation with all microorganisms in the red complex, and with F. nucleatum, P. nigrescens, Prevotella intermedia, and Eubacterium nodatum (p<0.05). Conclusion: P. gingivalis, C. rectus, and F. nucleatum exhibit strong interactions. Removing these bacteria can block complex formation and enhance the prevention and treatment of periodontitis and peri-implantitis.

Growing Response of Cyrtomium falcatum and Rumohra aristata Indoor as Influenced by Light Quality Treatment (실내에서 광질이 도깨비고비와 가는쇠고사리의 생육에 미치는 영향)

  • Bang, Kwang-Ja;Ju, Jin-Hee;Kwon, Min-Hoon
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.7 no.6
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    • pp.49-53
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    • 2004
  • This study was aimed to promote evergreen ferns native to Korea as a material for interior landscape by investigate effects of light quality on the growth of Cyrtomium falcatum and Rumohra aristata, in an indoor environment that artificial light was used, especially. Result of experiments are as follows; 1. Wavelengths were measured as control(=570~580nm), red(=600~610nm), yellow(=550~580nm), green(=500~510nm) and blue(=430~440nm) between different color film. The order of photon flux density was red>yellow>control>green>blue decreased. 2. Although there was no difference in the growth of Cyrtomium falcatum depending on light quality, in case of fronds with sori and new fronds, there were highest under red film. Fresh weight was no significant in all treatments, but dry weight was increased with green>control>yellow>blue>red in order. 3. In case of Rumohra aristata, there was no difference in its growth, however, number of total fronds was highest under green film. Although fresh weight was increased with yellow film, dry weight was highest under green film.

Morphological Traits of Lotus japonicus (Regal) Ecotypes Collected in Japan

  • Hashiguchi, Masatsugu;Tsuruta, Shin-Ichi;Akashi, Ryo
    • Interdisciplinary Bio Central
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    • v.3 no.1
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    • pp.4.1-4.7
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    • 2011
  • Forty-seven wild accessions of Lotus japonicus Regal (Japanese trefoil) indigenous to Japan were investigated for nine morphological characters. Average temperature and annual precipitation were negatively correlated with stem color and seed weight. On the other hand, latitude was positively correlated with these traits. Consequently, accessions from sites at higher latitudes with low temperatures and precipitation tend to have dark red stems and heavy seeds. Cluster analysis based on nine morphological characters classified 47 wild accessions into six major groups. Cluster I included four accessions of tall and erect plants. These plants are phenotypically similar to commercial variety 'Empire'. Cluster II consisted of three accessions of creep plants with pale red stems. Cluster III contained 24 accessions that had average values for all morphological characters evaluated. Cluster IV included two accessions of erect plants with rounded leaflets and dark red stems. Cluster V included four accessions of small, creep plants with pale red stems. Cluster VI included seven accessions of small and erect plants, a phenotype that also applies to ?Gifu B-129?, which is used as experimental strain worldwide. These data were deposited into LegumeBase, an online database (http://www.legumebase.brc.miyazaki-u.ac.jp/) supported by the National BioResource Project (NBRP) in Japan.

Distribution, habitat characteristics, and diet of freshwater turtles in the surrounding area of the Seomjin River and Nam River in southern Korea

  • Lee, Heon-Joo;Park, Dae-Sik
    • Journal of Ecology and Environment
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    • v.33 no.3
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    • pp.237-244
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    • 2010
  • In this study, we evaluated the distribution, habitat characteristics, and diet of two Korean freshwater turtle species (Chinemys reevesii, Pelodiscus sinensis) and an invasive turtle species (Trachemys scripta elegans) in the area surrounding the Seomjin River and the Nam River. We surveyed basking turtles in multiple locations along a 48-km stretch of the Seomjin River and in 99 reservoirs distributed along the Seomjin and Nam rivers from June to September, 2009. We observed 8 and 6 red-eared turtles in 3 reservoirs and at 3 sites in the Seomjin River, respectively, and 33 Reeve's turtles in 9 reservoirs. There were also 28 and 16 mud turtles detected at 15 sites along the Seomjin River and in 8 reservoirs, respectively. Among the 14 biotic and abiotic habitat characteristics that might influence the abundance of freshwater turtles in reservoirs, only the distance between a reservoir and the nearest residential areas was correlated negatively with abundance. With regard to the diet, all Reeve's and red-eared turtles investigated were determined to forage on water snails. Some turtles also foraged on vegetation and aquatic invertebrates. Additionally, we found fish in the stomach of one of the Reeve's turtles, and dragonflies in the stomachs of two red-eared turtles.

Physicochemical and Sensory Characteristics of Kakdugi Prepared with Red Paprika Powder (홍파프리카로 담근 깍두기의 이화학적.관능적 특성)

  • 이지숙;이영주;김미리
    • Journal of the East Asian Society of Dietary Life
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    • v.13 no.1
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    • pp.64-71
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    • 2003
  • Physicochemical and sensory characteristics of Kakdugi substituted red pepper powder with red paprika powder during fermentation at 1$0^{\circ}C$ were compared with those of the control. The fermentation patterns of two Kimchies were similar. While the pH and the acidity of the control were 3.88 and 0.691%(as lactic acid), respectively, those of paprika, 3.85 and 0.690%(as lactic acid), respectively on the 10th day of fermentation, which is the optimal ripe time. Reducing sugar content and Lactobacilli number of paprika Kakdugi were higher than those of the control. The hardness and fracturability of Kakdugi radish were not significantly different from those of the control during fermentation. The Hunter color L, a and b values of both Kakdugies increased gradually until l0th day and then decreased. The a and ASTA value of paprika Kakdugi was higher than that of control, which is consistent with the results of sensory evaluation. Capsaicin and dihydrocapsaicin analyzed by HPLC were not detected in paprika Kakdugi. Sensory evaluation (unstructured scale, 10 points) showed the score of hot taste f3r paprika Kakdugi was lower than that of the control(p<0.05), whereas the score of over-all acceptabillity for paprika Kakdugi(8.0) was higher than those of control(6.1).

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STudies on the Microbial Pigment(I) (미생물의 색소에 관한 연구. 제1보)

  • Ahn, Tae-Seok;Choi, Yong-Keel;Hong, Soon-Woo
    • Korean Journal of Microbiology
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    • v.15 no.4
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    • pp.159-169
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    • 1977
  • The bacteria of red colonies isolated from soil were identified as Serratia marcescens. The best solvent for pigment extraction was n-buthanol and the pigment was identified as prodigiosene. The extracted pigment was stable on temperature and light but not on acidity. The redpigment color changed into red in alkaline solution. The maximum absorbancy of pigment was 466 nm in alkaline condition and 540 nm in acid condition. And the pigment formed single spot on the TLC(starch). By the result of infra red spectrum, the red pigment has the same absorption pattern comparing with, the prodigisin produced by S. marcescens strain Nima. It was confirmed that the pigment was secondary metabolite and that the maximal peak of production appeared at 30 hrs after the inoculation, when the bacterial growth was in statinary state. Referring to the effect of temperature, the pigment was not formed at $36^{\circ}C$ and the optimal temperature for both of bactrial growth and pigmentation was $30^{\circ}C$. The optimal range of pH for pigmentation was 5.0 and under the condition the bacterial growth was not affected at all. Examining the effects of light, the bacterial pigment ation was more increased in darkness than in visible light.

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Characteristics of Colors and Color Communication tools applied in the Korean Fashion Industry (국내 패션의류업계의 활용 색채전달도구 개발을 위한 색채 연구)

  • 김영인;조민정
    • Journal of the Korean Society of Costume
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    • v.50 no.4
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    • pp.53-61
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    • 2000
  • The purpose of this study is to classify the characteristics of color communication tools and the color range applied in the Korean fashion industry. To collect the color sample and related references, a questionnaire was distributed during the time period, December, 1995 to June, 1996. From the responses, 2641 color samples used by 109 domestic brands, from 1993 spring/summer to 1996 spring/summer, were collected and analyzed. The data was measured by L*a*b*, and the H V/C formula found in the Munsell notation was used to calculate the data. To classify the color range served for the fashion industry, the distribution of colors are analyzed by difference of season and fabric. The results of this study are as follows : 1. Dominant colors in the Korean fashion industry are Red, Yellow-red, Yellow, purple-blue and low chromatic range colors. 2. While high value colors, such as pale, light greyish, light, dull, dark and vivid tone colors, are dominant in the spring/summer season, low value colors, such as greyish, dark greyish. and deep tone colors and warm colors such as Red, Yellow-red, are prevalent in the fall/winter season. 3. The number of colors commonly used for color planning is less than 20 colors, and both hue and tone are considered important when making color selections. 4. All brands take consumers' color preferences into consideration for color planning, and most of them also take color trends into account. 5. Hue and tone color characteristics analyzed by types of fabrics show more seasonal influence than the fabric itself.

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Cochlodinium Red Tide Effects on the Respiration of Abalone, Haliotis discus hannai Ino

  • Seo, Kyung-Suk;Lee, Chang-Kyu
    • ALGAE
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    • v.22 no.3
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    • pp.241-246
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    • 2007
  • Cochtodinium votykrikoides -related red tide is the most notorious tidal bloom, resulting in mass mortality to marineanimals. This study aimed to test the effect of C. polyknkoides on the lethality to Haliotis discus hannai under con-trolled conditions. The oxygen demand of C. polykrikoides increases to reach its peak duhng the night, while the oxy-gen usage by H. discus hannai was continuously decreased with a threshold of 2 mg L U. The addition of C.polykrikoides did not effect the respiration of the H. discus hannai. However, the usage of oxygen by C. polykrikoidesduhng the night may lead to anoxia in the animal. With aeration, the level of dissolved oxygen (D.O.) was between6.06 and 7.28 mg LU; 90% of abalones survived even with a high concentration of C. potykrikoides (9000 cells mL U).Without aeration (3 mg LU of D.O.), however, the H. discus hannai suffocated immediately. Once 20 hours hadelapsed, all of the abalones were dead. The density of the H. discus hannai population contributed to their mortality.Therefore, aeration during the night and maintaining lower abalone densities is the best way to promote the sur-vivorship of H. discus hannai during a C. polykrikoides red tide.

Manufacture of Colored Hanji for Interior Materials from Natural Pigments (Part 3) - Functional Properties of Super Eight Colors Hanji Wallpaper - (천연색소를 이용한 건축내장용 색한지 제조 (제3보) - Super Eight Color 벽지의 기능성 연구 -)

  • Lee, Sang-Hyun;Shin, Yoo-Su;Choi, Tae-Ho
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.47 no.5
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    • pp.120-126
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    • 2015
  • In this study, we used various dyes from natural pigments such as tumeric, goldthread, indigo, pagoda tree flower, sappanwood, and safflower to make the Hanji wallpaper with super eight colors of yellow, orange, green, turquoise, violet, red, blue, and magenta. As a result of measuring the lightfastness, magenta dyed with sappanwood showed the worst effect, but blue dyed with only indigo showed the best effect. In terms of the spot test by water, red wallpaper dyed with safflower showed the clear spot, but blue, turquoise, and green dyed with indigo resulted in a little spot. The orange, violet, and magenta dyed with goldthread and sappanwood showed antibacterial activity, and the turquoise, green, and blue also has a little antibacterial activity. However, yellow and red didn't show the some result as expected. In the experiment for efficiency of formaldehyde removal, all Hanji wallpapers dyed with natural pigments showed the significant removal effect of formaldehyde, especially turquoise and red were the highest.

A Study on the Carbamate Pesticide Residues in Domestic and Imported Crops in Chonnam Province (전라남도에서 유통중인 국내산 및 수입산 곡류 중의 카바메이트제 농약 잔류 실태 연구)

  • 김영국
    • The Korean Journal of Food And Nutrition
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    • v.13 no.2
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    • pp.164-170
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    • 2000
  • 10 carbamate pesticides were surveyed in domestic and imported green peas, beans, red beans from March 1999 through December 1999. Samples were collected from Gwangju, Mokpo, Suncheon and Yosoo grain markets. This study was performed by post-column ο-phthalaldehyde and 2-mercaptoethanol prior to HPCL fluorophore detection. Pesticides were found in 14 of 80 samples(17.5%). The kinds of pesticide detected in green peas were methomyl, MTMC, carbaryl, those in sesames were oxamyl, carbofuran, carbaryl, 1-naphthol, MIPC, those in beans were oxamyl, MTMC, carbaryl and those in red beans were MTMC, carbaryl. The range of residues detected in green peas, sesames, beans and red beans were 0.002∼0.025ppm, 0.001∼0.469ppm, 0.005∼0.356ppm and 0.017∼0.125ppm, respectively. The most frequently detected pesticides were MTMC(6 times) and carbaryl(5 times), while aldicarb, PHC and BPMC were no detected in all samples. Detection frequencies of carbamate pesticides in imported crops were higher than those in domestic ones except red beans. Only one result of oxamyl residues determined in imported bean was higher than the proposed maximum residue limits of Codex, which was 0.356ppm.

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