• Title/Summary/Keyword: Alkaline phosphatase

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Hydroxyapatite Solubilization and Organic Acid Production by Enterobacter agglomerans (인산염 가용화균 Enterobacter agglomerans에 의한 Hydroxyapatite 가동화와 유기산 생성)

  • Kim, Kil-Yong
    • Korean Journal of Soil Science and Fertilizer
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    • v.30 no.2
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    • pp.189-195
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    • 1997
  • A phosphate solubilizing bacterium (PSB) possessing a high ability to solubilize hydroxyapatite (HA) was isolated from the rhizosphere of wheat. The PSB markedly developed clear zones after inoculating for 36 hours at $30^{\circ}C$. This bacterium was identified as Enterobacter agglomerans through API 20E system and Biolog$^{TM}$ analysis. The values of similarity and distance coefficient from authentication trial of the strain were 0.656 and 4.79 respectively. High performance liquid chromatography (HPLC) of the products of this strain indicated that this strain excretes maily oxalic acid with som other organic acids. During the incubation period of E. agglomerans, the pH values showed an inverse correlation ($r^2=0.933^{**}$) with solubilization of inorganic phosphate. Acid phosphatase activity of the strain was 10-15 times greater than alkaline phosphatase activity. Alkaline phosphatase activity had almost constant near zero activity across time. The population of E. agglomerans greatly increased during the first day of inoculation ; however, it drastically decreased thereafter.

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Enzyme-Htstochemical Studie5 of Esterase and Phosphatase on Developing Spirometra erinacei (Spirometra ernacei의 발육에 따른 Esterase와 Phosphatase의 조직 화학적 연구)

  • 곽기훈;김창환
    • The Korean Journal of Zoology
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    • v.31 no.3
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    • pp.225-235
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    • 1988
  • This study was carried out to compare distribution and isozyme pattern of nonspecific esterase, acid phosphatase and alkaline phosphatase on developing sparganum and adult of Spinometra erinacei by using enzyme-histochemical method and electrophoresis The sparganum and adult were recovered from rats and cat that were infected by sparganum. The results obtained were as follows: Nonspecific esterase had a strong activity in the parenchymal musculature of sparganum and adult, but no detectable level in ihe tegument. A total of 7 and 8 nonspecific esterase bands were detectable in sparganum and adult, respectively. Of these bands, band 3 and 4 were major bands in sparganum and adult. Acid phosphatase had a strong activity in the tegument and the epidermal musculature of sparganum, but no detectable level in the parenchymal musculature. A total of 3 bands were detectable in sparganum and adult. Of these bands, band 3 was major band in sparganum and adult. Alkaline phosphatase had a strong activity in the tegument and the epidennal musculature of sparganum and of adult, but no detectable level in the parenchymal musculature. A total of 2 and 4 bands were detectable in sparganum and adult. Of these bands, band 2 was major band in sparganum and adult. Based on the present results isozyme band patterns showed qualitative and quantitative changes in each tissues of sparganum and of adult during the development.

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Structural Analysis of Alkaline Phosphatase Gen from Kluyveromyces Fragilis (Kluyverromyces fragilis의 Alkaline Phosphatase 유전자의 구조 분석)

  • Park, Soo-Young;Hwang, Seon-Gap;Ha, Sang-Chul;Kim, Jong-Guk;Park, Wan;Hong, Soon-Duck
    • Microbiology and Biotechnology Letters
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    • v.22 no.1
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    • pp.31-36
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    • 1994
  • From the pSKH201 plasmid which had been previously cloned in our laboratory, a 3.0kbp insert DNA encoding the alkaline phosphatase of Kluyveromyces fragilis was cleaved with several restriction endonucleases and ligated int the appropriate sites of M13mp18/19 vectors and sequenced by Sanger's dideoxy chain termination method. The sequence contained a 1,638 bp open reading frame(ORP) whose similarities in nucleotide, when compared with those of Saccharomyces cerevisiae and Escherichia coli by GENETYX program, were found to be 61% and 46%, respectively. The deduced amino acid sequence consists of 546 amino acids and contains several homologous regions in the alkaline phosphatases of E. coli, S.cerevisiae and human placenta.

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EFFECTS OF ALENDRONATE AND PAMIDRONATE ON THE PROLIFERATION AND THE ALKALINE PHOSPHATASE ACTIVITY OF HUMAN BONE MARROW DERIVED MESENCHYMAL STEM CELLS (Alendronate와 Pamidronate가 인간 골수유래 간엽줄기세포의 증식과 알칼리성 인산분해효소 활성에 미치는 영향)

  • Kim, Young-Ran;Ryu, Dong-Mok;Kwon, Yong-Dae;Yun, Yong-Pil
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.35 no.6
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    • pp.397-402
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    • 2009
  • The purpose of this study is to investigate the effects of alendronate and pamidronate on proliferation and the alkaline phosphatase activity of human bone marrow derived mesenchymal stem cells and to relate the results with bisphosphonate related osteonecrosis of the jaw(BRONJ). With the consent of patients with no systemic disease and undergoing iliac bone graft, cancellous bone was collected to obtain human bone marrow derived mesenchymal stem cells through cell culture. 96 well plate were prepared with a concentration of $10^4$cell/ well. Alendronate and pamidronate were added to each well with the concentration of $10^{-6}M$, $10^{-8}M$ and $10^{-10}M$, respectively. Then proliferation capacity of each well was evaluated with the cell counting kit. 24 well plates were prepared with a concentration of $10^5$cell/ml/well and with the bone supplement, alendronate and pamidronate were added with the concentration of $10^{-6}M$, $10^{-8}M$ and $10^{-10}M$, respectively on each plate. The plates were cultured for either 24 or 72 hours. Then the cells were sonicated to measure the alkaline phosphatase activity and protein assay was done to standardize the data for analysis. As the concentration of alendronate or pamidronate added to the culture increased, the proliferation capacity of the cells decreased. However, no statistical significance was found between the group with $10^{-10}M$ of bisphophonate and the control group. Pamidronate was not capable of increasing the alkaline phosphatase activity in all trials. However, alkaline phosphatase activity increased with 24 hours of $10^{-8}M$ of alendronate treatment and with 48 hours of $10^{-10}M$ of alendronate treatment. Cell toxicity increased as the bisphosphonate concentration increased. This seems to be associated with the long half life of bisphosphonate, resulting in high concentration of bisphosphonate in the jaw and thus displaying delayed healing after surgical procedures. Alendronate has shown to increase the alkaline phophatase activity of human bone marrow derived mesenchymal stem cells. However, this data is insufficient to conclude that alendronate facilitates the differentiation of human bone marrow derived mesenchymal stem cells. Further studies on DNA level and animal studies are required to support these results.

Hepatic Cell Membrane Changes of Rats in the Early Postmortem Period

  • Yoon, Hyung-Won;Yoon, Chong-Guk;Cho, Hyun-Gug
    • Biomedical Science Letters
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    • v.8 no.2
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    • pp.89-93
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    • 2002
  • To investigate the postmortem changes in hepatic cell membrane, the rats were sacrificed with cervical dislocation and kept in an incubator at $25^{\circ}C$, 70% of humidity for 12 hours. The biochemical experiments in postmortem were done at 2, 4, 8 and 12 hours. The degree of rigor mortis and algor mortis were increased with the time during 12 hours. The contents of hepatic malondialdehyde were rapidly increased ai 2 hours, and gradually decreased afterward. In histological findings, after 8 hours, the clotted blood was seen in central vein and sinusoids, and especially portal veins were dilated a1though the structure of hepatic lobules was preserved well. Furthermore, both in the histochemical and enzymatic examinations, membrane bounding alkaline phosphatase activities were gradually decreased with the time. In conclusion, the activity of membrane bounding alkaline phosphatase was linearly decreased with time in the early postmortem period and so it might be referred to the possibility fur the estimation of death time in the early postmortem period.

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한국 진도개와 삽사리 혈액 단백질의 비교연구 II. 혈청 Lactate Dehydrogenase와 혈청 Alkaline Phosphatase의 동위효소와 활성도

  • 김종봉;윤인숙
    • The Korean Journal of Zoology
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    • v.35 no.1
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    • pp.102-106
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    • 1992
  • 진도개와 삽사리 혈청 lactate dehydrogenase와 혈청 alkaline phosphatase의 동위효소 및 효소활성도를 분석하였다. 전기영동결과 진도개와 삽사리의 혈청에서는 5두지 종류의 LDH의 동위효소가 모두 확인되었다. LDH의 활성도는 진도개의 경우 522.53 $\pm$ 279.96(U/L)이었고 삽사리는 534.10 $\pm$ 280.35(U/L)이었다. 진도개와 삽사리의 혈청 alkaline phosphatase전기 영동상에서 는 한 종류의 동위효소만 관찰되었고 활성도는 진도개의 경우 7.61 $\pm$ 4.52(K-A unit)였고 삽사리는 10.46 $\pm$ 7. 10(K-A unit) 였다. 삽사리의 ALP 활성도는 연령에 따라 커다란 차이를 나타내었다.

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Enhancement of Immune Responses by a Water Soluble Proteoglycan, Lepidan from Lentinus lepideus (잣버섯 균사체로부터 분리한 수용성 단백다당체 Lepidan의 면역 증가 작용)

  • 진미림;정규선
    • YAKHAK HOEJI
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    • v.43 no.5
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    • pp.635-641
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    • 1999
  • In this study, we investigated the immunopotent activities of lepidan, a water soluble proteoglycan from Lentinus lepideus. To examine whether lepidan may affect nonspecific immune responses, we determined the effect on the production of nitric oxide (NO). Lepidan effectively increased the NO production in IFN-${\gamma}$ and LPS triggered RAW 264.7 cells. To further demonstrate the evidence that lepidan activates various immune cells, we determined the alkaline phosphatase activity, plaque-forming cell number and secretion of interleukine-4 (IL-4) and granulocyte/macrophage-colony stimulating factor (GM-CSF) after lepidan treatment in murine splenocytes. The results showed that lepidan increased alkaline phosphatase activity and the number of plaque-forming cells, which indicates that lepidan can lead B lymphocytes to late stage of differentiation. Also, when the splenocytes were cultured with lepidan for 48 hr, the level of IL-4 and GM-CSF in the supernatant dramatically increased. Taken together, these data suggest that lepidan is a biological response modulator that is able to activate immune responses.

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AN EXPERIMENTAL STUDY OF PHOSPHATASE ACTIVITY IN PERIAPICAL GRANULOMA (치근단 육아종의 Phosphatase 활성에 관한 실험적 연구)

  • Yu, Gwang-Hui
    • The Journal of the Korean dental association
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    • v.13 no.6
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    • pp.529-531
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    • 1975
  • This observation was carried out to investigate the phosphatase activity and the calcium contents of periapical granuloma in patients of both sex and different age. The results were as follows : 1. Acid phosphatase activity was considerably increased with bone absorption. 2. Alkaline phosphatase activity was also remarkably increased in periapical granuloma. 3. In case of periapical granuloma, differences of phosphatase activity by age and sex were not observed. 4. Calcium contents in periapical granuloma was of very small quantity, showing remarkable decrease when compared with the normal bone tissue.

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The Effects of Phosphate Starvation on the Activities of Acid and Alkaline Phosphatase, Fructose-1,6-bisphosphatase, Sucrose-phosphate Synthase and Nitrate Reductase in Melon (Cucumis melo L.) Seedlings

  • Kang, Sang-Jae;Lee, Chang-Hee;Park, Man
    • Korean Journal of Soil Science and Fertilizer
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    • v.49 no.1
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    • pp.44-52
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    • 2016
  • Plants response to phosphate starvation include the changes of activity of some enzymes, such as phosphatases, fructose-1,6-bisphosphatase, sucrose-phosphate synthase and nitrate reductase. In this study, to determine the effects of phosphate starvation on the change of activities of acid and alkaline phosphatase, fructose-1,6-bisphosphatase, sucrose-phosphate synthase, and nitrate reductase were studied in melon seedlings (Cucumis melo L.). The content of the protein and chlorophyll tended to relatively reduced in melon seedlings subjected to phosphate starvation. Acid phosphatase activity in first and second leaves of melon seedlings was relatively higher than that of third and fourth leaves of seedlings in 14 days after phosphate starvation treatment, respectively. Active native-PAGE band patterns of acid phosphatase in melon leaves showed similar to activities of acid phosphatase, whereas alkaline phosphatase activity was different from the change in the activity of acid phosphatase. Inorganic phosphate content in melon seedlings leaves was constant. The changes of Fructose-1,6-bisphosphatase and sucrose phosphate synthase activities showed similar patterns in melon seedlings leaves, and between these enzymes activities and phosphate nutrition negatively related. Fructose-1,6- bisphosphatase and sucrose phosphate synthase activities showed significant difference in second and fourth leaves, but nitrate reductase showed significant difference in first and second leaves in 14days after phosphate starvation treatment. We concluded that phosphate nutrition could affect the distribution of phosphate, carbon and nitrogen in melon seedlings.

THE EFFECTS OF MECHANICAL STRESS ON CULTURED BONE CELL POPULATIONS (Mechanical stress가 골조직세포군에 미치는 영향)

  • Kim, Sang-Tae;Cha, Kyung-Suk
    • The korean journal of orthodontics
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    • v.24 no.1 s.44
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    • pp.105-114
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    • 1994
  • The movement of teeth during orthodontic treatment requires bone remodeling process of bone formation and bone resolution. To find out the changes occuring in the cell itself, mechanical stress was applied to the cell populations involved in the bone metabolism. Bone tissue cell populations were isolated from fetal rat calvaria and divided into OC and OB groups. Following results were obtained from measuring the changes in acid & alkaline phosphatease activity, cyclic AMP and $PGE_2$ production in time lapse after the application of mechanical stress. 1. In case of the marker enzyme of specific bone tissue cell, acid phosphatase activity was high in OC group and alkaline phosphatase activity was high in OB group. 2. After the mechanical stress was applied, acid phosphatase activity was decreased in both OC and OB groups and alkaline phosphatase activity was increase in OB group. 3. When the mechanical stress was applied for 15, 30 and 60 minutes, the production of $PGE_2$ increased in both OC and OB groups, as the time span increased. 4. When the mechanical stress was applied for 20 and 40 minutes, the production of $PGE_2$ increased in both OC and OB groups, as the time span increased.

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