• Ocean and Polar Research
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• 제25권4호
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• pp.625-631
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• 2003

While phytoplankton diversity and productivity in the Southern Ocean has been widely studied in recent years, most attention has been given to elucidating environmental factors that affect the dynamics of micro-plankton (mainly diatoms) and nano-plankton (mainly Phaeocystis antarctica). Only limited effects have been given to studying the occurrence and the potential risks associated with the blooming of dinoflagellates in the relevant waters. This study focused on the appearance and toxicological characteristics of a toxic dinoflagellate, Alexandrium tamarense, identified and isolated from the Drake Passage in a research cruise from November to December 2001 The appearance of A. tamarense in the Southern Ocean indicates the risk of a paralytic shellfish poisoning (PSP) outbreak there and is therefore of scientific concern. Results showed that while the overall quantity of A. tamarense in water samples from 30meters below the sea surface often comprised less than 0.1% of the total population of phytoplankton, the highest concentration of A. tamarense (20 cells $L^{-1}$) was recorded in the portion of the Southern Ocean between the southern end of South America and the Falkland Islands. Waters near the Polar Front contained the second highest concentrations of 10-15 cells $L^{-1}$. A. tamarense was however rarely found in waters near the southern side of the Polar Front, indicating that cold sea temperatures near the Antarctic ice does not favor the growth of this dinoflagellate. One strain of A. tamarense from this cruise was isolated and cultured for further study in the laboratory. Experiments showed that this strain of A. tamarense has a high tolerance to temperature variations and could survive at temperatures ranging from $5-26^{\circ}C$. This shows the cosmopolitan nature off. tamarense. With regard to the algal toxins produced, this strain of A. tamarense produced mainly C-2 toxins but very little saxitoxin and gonyailtoxin. The toxicological property of this A. tamarense strain coincided with a massive death of penguins in the Falkland Islands in December 2002 to January 2003.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2002년도 생물공학의 동향 (X)
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• pp.112-115
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• 2002

본 연구에서는 균주의 배양과 동시에 생산물을 회수할 수 있는 동시추출공정을 건조질량의 $15{\sim}75$ %의 탄화수소를 생산한다고 알려진 B. braunii 배양에 적용하고자 한다. 일반적인 tow-phase 동시 추출공정의 적용시 B. braunii의 경우 생산된 탄화수소가 균주 외벽의 matrix에 강하게 부착되어 있기 때문에, two phase 추출공정 적용시 bubble solumn내에서 단지 폭기에 의한 교반만으로는 충분한 탄화수소의 회수율을 얻을 수가 없었다. 본 연구에서는 배양액과 유기용매층의 접촉기회를 증대시킨 two-stage 동시추출 공정을 개발하여 기존의 two-stage 동시추출 공정보다 2배 이상 높은 57 %의 탄화수소 회수율을 얻을 수가 있었고, 이를 회분배양후 후속분리공정으로 이용할 경우 6시간 추출후 62 %의 회수율을 얻을 수가있었다.

• 생태와환경
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• 제38권2호통권112호
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• pp.181-187
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• 2005

한강 중하류의 남조류 및 환경요인의 연간변화 (2000 ${\sim}$ 2003)를 파악하기 위하여, 2000년 4월부터 2003년 10월까지 4년간 성수대교에서 성산대교까지 총 5개 지점을 선정하여, 수계의 기초환경요인 및 식물플랑크톤 현존량 변화를 조사하였다. 조사기간 동안, 남조류의 현존량은 점차적으로 감소하여 장기간 강우가 가장 많았던 2003년에는 거의 관찰되지 않았으며, 총 식물플랑크톤 현존량에 대한 남조류의 기여도는 약 10%범위내였다. 이러한 현상은 매년 강우집중기 동안 팔당댐으로부터 많은 방류수가 유입됨으로서 수온감소는 물론 광량감소, 영양염류 희석 등에 의한 남조류의 성장이 제한된 것으로판단되며, 동일시기에 다른 조류 (녹조, 규조 등)의 높은 성장으로 엽록소 a와 식물플랑크톤 현존량이 뚜렷하게 증가하였다. 따라서 한강 하류의 고온기 동안 남조류 성장은 잦은 강우와 방류에 의해 제한을 받지만 규조나 녹조와 같은 다른 조류들의 높은 성장으로 대치되는 천이현상을 보였으며, 기후영향을 크게 받는 전형적인 하천생태계의 특성을 나타냈다.

• 한국해양학회지:바다
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• 제21권2호
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• pp.58-66
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• 2016

해수 내 용존 암모늄이온의 농도 자료가 해양환경의 지표로서 가치가 있는지 평가하였다. 먼저 암모니아의 화학적인 특성을 정리하였고, 본론에서 해양환경의 대표성을 띠는 일반해역(원양, 연안, 자연하구역)과 집중비교해역(골재채취해역, 하구역)에서 암모늄 자료의 가치를 비교하였다. 암모늄은 일반해역에서 전반적으로 DO와 음의 상관관계를 갖고, COD와 양의 상관관계를 보임으로써 수질지표로서 광범위한 적용 가능성을 보였다. 특히 DO 또는 COD가 유사한 농도를 나타내는 해양환경조건의 경우, 보다 상세한 해석이 불가능하였으나 ODIN 비율 (산화성용존무기질소: ODIN/TDIN ratio) 혹은 RDIN 비율 (환원성용존무기질소: RDIN/TDIN ratio)를 적용하면, 비교대상 해역의 산화 및 환원 조건에 대한 보다 향상된 정보를 얻을 수 있었다. 집중비교해역에서는 저층퇴적물의 확산, 식물플랑크톤의 단기변화 등 주로 단주기 환경변화와 관련하여 암모늄의 지표로서 잠재력을 확인하였다. 암모늄은 기존의 몇 가지 단점을 보완한다면 해양환경에서 단주기로 변화하는 해양한경을 이해하는데 도움이 될 것으로 판단된다.

• 한국물환경학회지
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• 제35권4호
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• pp.340-351
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• 2019

In this study, the relationship between trophic state indices was analyzed based on the monthly or weekly water quality data of 81 lakes (mostly man-made) in Korea between 2013-2017. Carlson's $TSI_C$ and Aizaki's $TSI_m$ were calculated using the summer (Jun.-Sep.) average data at the upper water layer. The previous Korean trophic state index ($TSI_{KO}$) and the newly suggested index ($TSI_{KON}$) was calculated using the annual average data at the whole layer and at the upper layer, respectively. While previous trophic state index (TSI) such as Carlson's TSI included logarithmic function, we devised newly Monod-type $TSI_{KON}$(Chl) that is 50 when half-saturation concentration of chlorophyll ${\alpha}$ ($Chl.{\alpha}$) measured by UNESCO-method is $10{\mu}gL^{-1}$. MMF-type $TSI_{KON}$(TP) was derived based on the relationship between TP and $Chl.{\alpha}$. A comprehensive $TSI_{KON}$ was decided as the larger one of the two $TSI_{KON}$ values. The range of previous TSI was usually 40-50 for the mesotrophic state, which seemed narrow to discriminate trophic characteristics of the class. The upper limits of $TSI_{KON}$ for oligotrophic, mesotrophic, and eutrophic state were set to 23, 50 and 75, respectively. Classification by $TSI_C$ and $TSI_m$ showed higher frequency of eutrophic class compared to $TSI_{KO}$ and $TSI_{KON}$. This means that the estimation by TSIs developed in foreign natural lakes can lead to distorted results in the classification of the trophic state of Korean lakes. This is due to the decrease of transparency by non-algal material and the reduction in phosphorus availability to algal growth, particularly in Monsoon period.

• 한국환경복원기술학회지
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• 제7권5호
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• pp.75-84
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• 2004

Loess, PAC, MACF and plants were applied to the control of the phytoplankton bloom in laboratory and in field, In field experiment using oil fence, 5ppm concentration of coagulant(PAC) was observed to be effective in controlling the cyanobacterial bloom, resulting in 90% removal of cyanobacteria and phytoplankton from the water column, hi case of Synedra sp., however, only 50% of biomass decreased with the same PAC concentration. MACF(micro-air bubble coagulation and floating), a kind of physicochemical method, was applied to the column of the Kyongan stream and resulted in over 80% chlorophyll a and 73.5% TP removal, Chlorophyll a and total phosphorus were effectively removed from water body when 2.0 g/L of loess with the particle radius of 125 ${\mu}m$ was inputted. In case of experiments involving plants, big cone pine, gingko, and pine needle were observed to be effective in restraining phytoplankton bloom at 0.5g/200ml level. During a field test done at Kyungan stream, where Microcystis heavily occurred, Pine needle and big cone pine were observed to be effective on suppressing algal growth.

• 한국환경과학회지
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• 제26권3호
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• pp.393-399
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• 2017

In order to determine the optimal water intake point, the distribution of blue-Green algae and water quality factors in relation to the depth of the Mulgum and Maeri stations located downstream of the Nakdong River were investigated from Jun. 2015 to Sep. 2016. When the current surface water intake system was converted to the deep water intake system, Chl-a concentration and blue-Green algae were reduced by 64.1% and 80.5%, respectively. Microcystin-LR was reduced by 50% to 100%, while geosmin and 2-MIB of the odorant substances were reduced by 42.9% and 11.8%, respectively. The water quality factors such as pH, water temperature, TOC and COD were gradually decreased by 30% in deep water. Therefore, if we used the deep water intake system selectively in the summer season when blue-Green algae masses occur, the concentration of the influx of blue-green algae and its by-products can be expected to decrease, leading to reduced operation costs in tap water production and improved of raw water quality.

• Biotechnology and Bioprocess Engineering:BBE
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• 제6권3호
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• pp.194-199
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• 2001

Chlorella kessleri cultivated in artificial wastewater using diurnal illumination of 12h light/12h dark (L/D) cycles. The inoculum density was 10(sup)5 cells/mL and the irradiance in light cycle was 45$\mu$mol㎡s(sup)-1 at the culture surface. As a control culture, another set of flasks was cultivated under continuous illumination. Regardless of the illumination scheme, the total organic carbon (TOC) and chemical oxygen demand (COD) was reduced below 20% of the initial concentration within a day. However, cell concentration under the L/D lighting scheme was lower tan that under the continuous illuminating scheme. Thus the specific removal rate of organic carbon under L/D cycles was higher than that under continuous illumination. This result suggested that C. kessleri grew chemoorganotrophically in the dark periods. After 3 days, nitrate was reduced to 136.5 and 154.1mg NO$_3$-N/L from 168.1mg NO$_3$-N/L under continuous illumination and under diurnal cycles, respectively. These results indicate nitrate removal efficiency under continuous light was better than that under diurnal cycles. High-density algal cultures using optimized photobioreactors with diurnal cycles will save energy and improve organic carbon sources removal.

• 상하수도학회지
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• 제23권4호
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• pp.491-497
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• 2009

The purpose of this study is to propose a method of controlling redtide microbes which grow abundantly and form harmful algal bloom in eutrophic waterbody with yellow loess and hydrogen peroxide. In the laboratory test, hydrogen peroxide was applied to single species of C. polykrikoides and multispecies of redtide microbes. The seawater was evaluated by the pre-test analysis including chlorophyll-a, luminance and transmittance. The test results showed that both single and mixed species of redtide microbes could be controlled with the dose of 30mg $H_2O_2/L$. Residual hydrogen peroxide was completely decomposed with the addition of powdered yellow loess at 2g/L~10g/L. However, the decomposition rate of residual hydrogen peroxide for sintered granular yellow loess was relatively low compared to the use of powdered one. With the addition of dissolved oxygen concentration was increased at a rate of 0.013 mg DO/mg $H_2O_2$, which is a little lower than the one predicted theoretically. No evidence for any detrimental effects on Artemia, a type of brine shrimps, was shown up to the concentration of 100mg $H_2O_2/L$.

• 생태와환경
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• 제36권2호통권103호
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• pp.124-138
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• 2003

본 연구는 호소형과 하천형으로 크게 구분할 수 있는 주요 댐 호의 8년간 수질 및 수문조사자료를 이용하여(1990${\sim}$1998)조류발생 패턴과 수문학적 요인과의 관계를 분석하고자 하였다. 각 댐 호의 수온은 하천형이 호소형보다 계절별 변화폭이 큰 것으로 나타났다. pH는 호소형의 경우 겨울철에 낮고 여름철에 상대적으로 높은 수준을 보였으나, 하천형에서는 대부분 봄철과 가을철에 높고 여름철에 낮은 수준을 나타냈다. COD농도는 호소형 3개 댐 호 및 하천형 댐 호인 팔당호에서 낮고(1 mg/L내외), 금강하구언 및 낙동강하구언에서는 높았다(2${\sim}$3mg/L) 용존산소 농도는 하천형이 호소형보다 높은 값을 보였다. 용존산소포화도의 계절별 변화패턴은 하천형 댐 호의 경우높은 수준(80 ${\sim}$ 100%)을 유지하면서 계절별 변화폭도 그리 크지 않은 반면, 호소형에서는 늦봄 ${\sim}$ 초여름경 최고치 (93%)를 보이다가 겨울철까지 천천히 감소(46${\sim}$66%)하는 패턴을 나타냈으며, 각 호수 저수량의 계절적변화와 반비례하는 경향을 보였다. 총 질소와 총 인 농도는 호소형에서 낮았으며, 호소형은 계절별로 큰 변화가 관찰되지 않은 반면 하천형에서는 상대적으로 유입부하량이 많고 변동폭도 큰 것으로 나타났다. 하천형 호소에서도 팔당호는 금강하구언이나 낙동강하구언보다 매우 낮은 수준을 나타냈다. 연 평균치로 본 N/P 질량비는 호소형에서는 높고, 하천형에서는 낮았다 투명도와 부유물질 농도의 상관관계로 판단할 때 하천형에서의 투명도는 부유물질 농도에 의존하는 경향을 보였다. 클로로필 a 의 연평균 농도로 보았을 때 하천형에서 훨씬 높은 조류발생 패턴을 보였다. 계절별로 보면 호소형은 강우기가 끝난 늦여름에서 초가을 동안에 높은 수준을 보였으나, 하천형에서는 강우기와 겨울철을 제외하고는 불규칙하게 연중 조류발생이 관찰되었다. 호소형에서 강우와 클로로필 a 농도와는 정의 상관관계가 밝혀졌으며, 하천형에서는 유의성이 없었다.