• 환경생물
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• 제31권4호
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• pp.493-498
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• 2013

To investigate the historical record of Alexandrium spp. in southern coastal areas of Korea, two sediment cores were collected from Gamak Bay and Yeoja Bay. Germination experiments revealed that the ellipsoidal Alexandrium cysts isolated from Gamak Bay and Yeoja Bay are morphologically identical to a toxic dinoflagellate A. tamarense. The ellipsoidal Alexandrium cysts in Yeoja Bay appeared from 30 to 32 cm depth upwards (ca. 1980s), and their concentration increased around 10 to 12 cm depth (mid-1990s). Similarly, cyst concentration in Gamak Bay also increased from 40 to 44 cm depth (ca. 1990s). These results coincide with the reports of Paralytic Shellfish Poisoning caused by A. tamarense in 1980s and 1990s along the southeast coast of Korea.

• Ocean Science Journal
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• 제42권1호
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• pp.31-39
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• 2007

Distributional characteristics of dinoflagellate cysts in surface sediments were investigated in relation to environmental factors in the eastern part of Geoje Island, Korea. Samples were collected from 10 stations in February of 2004 and water temperature and salinity were measured in February, May, September and November of 2004. Total 30 taxa of dinoflagellate cysts were identified representing 19 genera, 28 species and 2 unidentified species. Among these dinoflagellate cysts, Brigantedinium spp. of which relative proportion in th e total dinoflagellate cysts was 23.5%, was the most abundant at all stations except St. 1, and was followed by Spiniferites bulloideus (8.6%), Lingulodinium machaerophorum (8.2%) and Diplopsalis lenticula (6.7%). In addition, ellipsoidal cysts of the genus Alexandrium (Alexandrium catenella - tamarense type) and Gymnodinium catenatum, known to be causative organisms for PSP, occurred with high concentrations. Scrippsiella trochoidea was also found; however, its cyst concentration was low. Generally, species composition in the study area was similar to these reported from Jinhae Bay and Busan Harbor and several dinoflagellate cysts reflected the eutrophic condition. Cyst distribution in th e eastern part of Geoje Island seems to be influenced by the Tsushima Warm Current flowing from the southwest. The mean water temperature was $12.0^{\circ}C$ in February, $14.7^{\circ}C$ in May, $20.9^{\circ}C$ in September and $17.2^{\circ}C$ in November, which was most favorable for Alexandrium spp. growth. The abundances of dinoflagellate cysts rang e d from 528 to 2,834 cysts/g dry sediment. Higher concentrations were recognized in sediments of west area of the Jisimdo than at other stations. The cyst composition of this area was closely related to these of Jinhae Bay and Busan Harbor from which currents flow into this area. Higher cyst concentration in the west area of Jisimdo might be due to formation of the gyre.

• Fisheries and Aquatic Sciences
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• 제4권3호
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• pp.120-129
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• 2001

Thirty-six dinoflagellate cysts, representing 15 genera were identified in the surface sediments obtained from the northwestern East China Sea. Three cyst morphotypes found in this survey have not previously been described in the East China Sea and adjacent waters: Seleno­pemphix sp. 2, Selenopemphix sp. 3 and Trinovantedinium sp. 1. In the northwestern East China Sea, Operculodinium centrocarpum, Spiniferites bulloideus and ellipsoidal cysts of Alexandrium were commonly observed. Moreover, it was recognized that the ellipsoidal cysts of Alexandrium, whose motile cells of A tamarense and/or A catenella are responsible to paralytic shellfish poisoning, distributed not only restricted to the coastal areas but also to the offshore stations far from the Changjiang River mouth.

• 환경생물
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• 제37권3호
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• pp.268-277
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• 2019

Severe damages will result in human society, when several different critical natural phenomena coincide. One example relates to the resting cysts of Alexandrium species (dinoflagellates that cause paralytic shellfish poisoning), which are preserved in surface sediments throughout Osaka Bay, Japan. These cysts have been found to accumulate particularly densely in shallow areas in the inner parts of Osaka Bay, where a tsunami caused by an earthquake could occur any time. Damage by a tsunami could cause a change of the coastal ecosystems at Osaka Bay including the resuspension of surface sediments containing resting Alexandrium tamarense cysts and the subsequent redistribution of the cysts in newly deposited sediment. Under certain environmental conditions, these cysts could germinate and form dense blooms, leading to paralytic shellfish poisoning. Such a scenario could also affect other coastal areas, including the southern coast of the Korean Peninsula.

• Fisheries and Aquatic Sciences
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• 제1권2호
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• pp.283-292
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• 1998

The community dynamics and distribution of dinoflagellates and their cysts were monitored monthly from June 1996 to May 1997 at six stations in Masan-Chinhae Bay, one of the famous bays for red-tide occurrence in Korea. The dinoflagellate standing crops ranged from the minimum of 306 cells $ml^{-1}$ in February to the maximum of 37,959 cells $ml^{-1}$ in May. The species causing massive blooms were Prorocentrum micans Ehrenberg and Ceratium furca (Ehrenberg) Claparede & Lachmann in July, Gymnodinium sanguineum Hirasaka in October, Alexandrium tamarense (Lebour) Balech in April and Proroeentrum minimum (Pavillard) Schiller in May. Twenty-seven taxa of dinoflagellate cysts were isolated, including 20 identified species and 7 unidentified species. The mean cyst abundance ranged from the minimum of 556 cysts $cm^{-3}$ in June to the maximum of 5,727 cysts$cm^{-3}$ in February. The spatial pattern of cyst distribution showed the gradual increase in abundance from offshore to inshore stations. The cyst genera of Protoperidinium, Gymnodinium, Serippsiella, Gyrodinium and Alexandrium were abundant throughout the year. The vertical distribution of cysts showed the surface miximum at the 0-2cm layer and the sub-surface maximum at the 2-4cm layer. Total abundance of cysts showed the most significant relationships with water temperature, and some minor relationship with dissolved oxygen, salinity, pH, phosphate and total inorganic nitrogen of surface and bottom layer in the water column. The number of dinoflagellate species decreased, while the abundance of cysts increased 4.5 times as compared with the observation of 10 years prior to the present study at the same stations of Masan-Chinhae Bay.

• 한국수산과학회지
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• 제23권6호
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• pp.468-474
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• 1990

휴안포자를 형성하는 과편모조류중 우리나라 남해동부와 동해남부의 연안수역 특히 진해만에서 1982년 이래 3월부터 9월에 걸쳐 적조를 일으킨 종은 8종이었으며 이들은 6종의 과편모조류와 2종의 녹색편모조류로 구성되었다. 계절별로는 이른 봄철에 Scrippsiella trochoidea와 Heterocapsa triquetra종이, 늦봄에 Heterosigma akashiwo가 그리고 여름철에는 Gyrodinium instriatum과 Pheopolykrikos hartmannii가 적조를 일으켰다. 한편 Cochlodinium sp.와 Alexandrium affine는 늦여름부터 가을철에 적조를 일으켰다. 이중 Heterosigma akashiwo는 매년 같은 곳에서 같은 시기에 발생하는 경향을 뚜렷히 나타냈으며 Cochlodinium sp.에서도 이와 같은 특성을 발견할 수 있었다. 한편 적조발생시의 밀도는 $10^3\~10^5\;cells/ml$ 수준이었으며, 밀도변화는 환경특성 보다는 오히려 세포의 크기에 따라 변화하고 있었다.

• 한국양식학회지
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• 제7권4호
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• pp.251-264
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• 1994

패독발생의 원인규명을 위한 모니터링의 일환으로 원인생물의 출현과 휴면포자의 발아에 의한 현장 개체군의 유입가능성을 조사하기 위하여 1993년 9월부터 진해만을 중심으로 Alexandrium속의 발생현황, 원인생물의 잠재력을 제공하는 저질중의 휴면포자의 분포 및 실내 발아율을 조사하였다. 만내의 조사된 모든 정점에서 1월부터 Alexandrium속이 출현하여 대곡리 정점(칠천도)에서 3월에 533 개체/l의 최고치를 보였으며, 다른 지점에서는 100개체 이하를 나타냈으나 수온상승기인 4월에는 출현량이 감소하였다. 저질중의 Alexandrium속 휴면포자의 분포밀도는 채집한 정점간의 차이가 많아 100-800 개체/$cm^3$의 밀도를 보였으나, 채집지역의 저질상부층$(0\~4cm)$에서 $700\~800$ 개체/$cm^3$의 분포 지점이 많았다 휴면포자의 발아율은 수정리 정점에서 1월$(72.5\%)$, 4월$(68.0\%)$, 7월$(44\%)$ 및 10월$(9.0\%)$ 순으로 채집 계절에 따른 차이를 나타냈으며, 1월 채집한 만내의 다른 2 정점의 휴면포자도 $71\~97|%$의 높은 발아율을 보였다. 한편, 수심이 얕은 다대포 지점의 휴면포자는 6월$(86.5\%)$과 10월$(70.0\%)$에도 높은 발아율을 보여 진해만의 각 지점과는 대조적인 결과를 보였다. 이상의 결과로부터 Alexandrium속 휴면포자의 발아는 지역환경에 따라 차이를 보일 수 있으며 비교적 수심이 깊은 지역에서는 온도 등의 환경요인의 변동 외에 내재적 발아리듬의 영향이 클 것으로 추정되었다. 따라서 진해만에서는 저수온기일지라도 휴면포자의 발아에 의한 Alexandrium속의 출현이 놀게 나타나며, 이 지역의 패독발생의 원인생물로 기여할 가능성이 높은 것으로 시사되었다.

• 생명과학회지
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• 제13권2호
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• pp.163-167
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• 2003

알렉산드륨 적조생물 속에서 마비성 패류독소를 생산하는 종을 신속하게 동정하므로 패류양식의 독성 모니터링과 방제에 중요한 역할을 할 수 있다. 자연상태에서 영양세포가 출현하기 전 알렉산드륨 타마렌스의 휴면포자만을 신속하게 분리 동정한다는 것은 근본적인 마비성 패류독소 모니터링 및 예측에 큰 역할을 할 수 있다. cTAM-Fl DNA probe은 알렉산드륨 타마렌스의 영양세포 뿐만 아니라 primuline으로 염색하여 메타놀로 고정한 휴면포자에도 반응이 되었다. 영양세포와 휴면포자에 반응되는 DNA probe 위치는 핵내의 말단 부위에 보였다. DNA probe가 세포내로 삽입되는데 가장 적합한 온도와 시간은 50-$54^{\circ}C$, 40-60분이 좋았다.

• ALGAE
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• 제23권4호
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• pp.251-255
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• 2008

In Gamak Bay, Paralytic Shellfish Poisoning (PSP) was first detected from seafoods in 2003, however the toxin source is unknown yet. In this study, we report potential PSP producers of toxic dinoflagellates, describing morphology and abundance of cysts isolated from surface sediment of Gamak Bay. The most abundant type in these cysts was characterized with ellipsoidal and transparent wall identical to Alexandrium catenella and/or A. tamarense. Germination experiment of the cysts revealed that all motile cells germinated were morphologically identified as A. tamarense. This result suggests that A. tamarense may relate to PSP contaminations in Gamak Bay. Moreover, bottom water temperature in Gamak Bay is favorable for germination of A. tamarense cysts. Further studies are required to carry out the PSP monitoring for preventing the risk of PSP events that may outbreak in future at Gamak Bay.

• ALGAE
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• 제26권1호
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• pp.41-50
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• 2011

Yellow Sea surface sediment samples collected on October 15-31, 2003 were analyzed using the palynological process to investigate the spatial distribution of dinoflagellate resting cysts. The sampling areas comprised four latitudinal transects, the northernmost of which was located off the Shandong Peninsula, China and the southernmost off Jeju Island, Korea. Each transect line was composed of six to nine stations, spanning the distance between the Chinese and Korean coasts. Twenty-five different types of dinoflagellate cysts were identified. Gonyaulax scrippsae, Alexandrium spp. (ellipsoidal type), and G. spinifera were the most dominant at all stations surveyed. Dinoflagellate cysts belonging to the Gonyaulacales comprised over 50% of all cysts collected. The latitudinal distribution trend showed that cyst concentrations along the two middle transects were much higher than those along the two northern and southern transects. Cyst concentrations in the offshore central areas reached their highest values within each transect and gradually decreased toward the Chinese and Korean coasts. Overall, cyst concentrations were markedly elevated in the offshore central Yellow Sea areas and gradually decreased outward in all four directions. This concentric cyst distribution pattern was consistent with the hydrographic features of the Yellow Sea, such as circular current systems, sedimentary properties, and water depth.