• 대한수의학회지
• /
• 제28권2호
• /
• pp.299-305
• /
• 1988

The phenotypes of hemoglobin, albumin and transferrin of 3U2 Jindo dogs in Jindo area were studied by starch gel electrophoresis for hemoglobin and albumin, and by polyacrylamide gradient gel electrophoresis for transferrin. The results obtained were as follows: 1. In the hemoglobin phenotypes, three phenotypes, HbAA, HbAB and HbBB, which were controlled by two allelic genes, $Hb^A$ and $Hb^B$, were observed and their frequencies of appearance were 1.65%, 10.60% and 87.75% respectively. The distribution of gene frequency was calculated as 0.0695 in $Hb^A$ and 0.9305 in $Hb^B$. 2. In the albumin phenotypes, three phenotypes, Alb FF, Alb FS and Alb SS, which were controlled by two allelic genes, $AIb^F$ and $AIb^S$ were observed and their frequencies of appearance were 12.59%, 25.56% and 61.85% respectively. The distribution of gene frequency was calculated as 0.2537 in $AIb^F$ and 0.7463 in $AIb^S$. 3. Analysis of transferrin phenotypes showed 6 different types which were controlled by three allelic genes, $Tf^B$, $Tf^C$ and $Tf^D$ and their frequencies of appearance were 54.04% in TfBB, 17.54% in TfBC, 9.82% in TfBD, 8.07% in TfCC, 7.37% in TfCD and 3.16% in TfDD. The distribution of gene frequency was calculated as 0.6772 in $Tf^B$, 0.2053 in $Tf^C$ and 0.1175 in $Tf^D$.

• Asian-Australasian Journal of Animal Sciences
• /
• 제33권3호
• /
• pp.416-423
• /
• 2020

Objective: This study examined the effects of divergence in residual feed intake (RFI) on expression profiles of key genes related to lipid transport in the liver and duodenal epithelium and their associations with feed efficiency traits in meat-type ducks. Methods: A total of 1,000 male ducks with similar body weight (1,042.1±87.2 g) were used in this study, and their individual RFI was calculated from 21 to 42 d of age. Finally, the 10 highest RFI (HRFI) and 10 lowest RFI (LRFI) ducks were chosen for examining the expression of key genes related to lipid transport in the liver and duodenal epithelium using quantitative polymerase chain reaction. Results: In the liver, expression levels of albumin (ALB), CD36 molecule (CD36), fatty acid hydroxylase domain containing 2 (FAXDC2), and choline kinase alpha (CHKA) were significantly higher in LRFI ducks than in HRFI ducks (p<0.01); negative correlations (p<0.05) between expression levels of ALB, CD36, FAXDC2, and CHKA and RFI were detected in the liver. Additionally, ALB expression was strongly positively correlated (p<0.05) with CD36, FAXDC2, CHKA, and apolipoprotein H (APOH) expression in the liver. In duodenal epithelium, we found that mRNA levels of ALB, CD36, FAXDC2, and APOH were significantly higher in LRFI ducks than in HRFI ducks (p<0.01); RFI was strongly negatively correlated (p<0.05) with ALB, FAXDC2, and APOH expression, while ALB expression was strongly positively correlated with APOH expression (p<0.01) in duodenal epithelium. Furthermore, expression levels of both ALB and FAXDC2 genes were significantly associated with feed conversion ratio and RFI in both liver and duodenal epithelium (p<0.05). Conclusion: Our findings therefore suggest that ALB and FAXDC2 genes might be used as potential gene markers designed to improve feed efficiency in future meat-type duck breeding programs.

• 농업과학연구
• /
• 제22권2호
• /
• pp.170-179
• /
• 1995

본 연구는 축협중앙회 한우개량사업소에서 사육하고 있는 한우 238두의 혈청내에 존재하는 혈액 단백질 및 효소의 유전적 다형을 조사하기 위하여, 혈청단백질인 post-transferrin-2(pTf-2), transferrin(Tf), post-albumin(pAlb) 및 albumin(Alb)과 혈청 효소인 ceruloplasmin(Cp) 및 amylase-I(Am-I), 그리고 혈구단백질인 hemoglobin(Hb)을 PAGE(polyacrylamide gel electrophoresis)와 STAGE(starch gel electrophoresis) 방법으로 유전적 다형을 분석하여 이들의 유전자형 및 유전자빈도를 추정하였던바 그 결과는 다음과 같다. 1. 혈청단백질인 pTf-2 좌위는 pTf-2 F와 S의 공우성 대립유전자가 검출되었고, 유전자형은 pTf-2 FF, FS 및 SS 형이 확인되었으며, 이들의 분포는 각각 46.22, 46.64 및 7.14%이었고, 유전자 빈도는 pTf-2 F와 S에서 각각 0.695 및 0.305이었다. 2. Tf 좌위는 Tf A, D1, D2 및 E의 대립유전자가 검출되었으며, 유전자형에 있어서는 Tf AA, ADl, AD2, AE, D1D1, D1D2, D2D2 DIE D2E 및 EE형이 확인되었고, 이들의 분포는 각각 0.84, 13.87, 13.03, 10.92, 22.27, 12.61, 2.94, 15.l3, 6.72 및 1.68%이었으며, 유전자빈도에 있어서는 Tf A, D1, D2 및 E의 빈도가 각각 0.197, 0.430, 0.191 및 0.181이었다. 3. pAlb 좌위는 pAlb F와 S 유전자가 검출되었으며, 유전자형의 분포에 있어서는 pAlb FFFS 및 SS 형이 각각 42.86, 33.19 및 23.95%이었고, 유전자빈도에 있어서는 pAlb F와 S에서 각각 0.595 및 0.405이었으며, Alb에서는 Alb A 유전자 빈도가 1.000이었다. 4. Cp 좌위는 Cp F와 S 유전자가 확인되었으며, 유전자형의 분포는 Cp FF, FS 및 SS 형에서 각각 23.11, 34.87 및 42.02% 이었고, Cp F와 S 유전자빈도는 각각 0.405 및 0.595이었다. 5. Am-I 좌위는 Am-I B와 C 대립유전자가 검출되었으며, 유전자형의 분포는 Am-I BB, BC 및 CC형에서 각각 51.26, 16.81 및 31.93%이었고, 유전자빈도는 Am-I B와 C가 각각 0.597 및 0.403이었다. 6. Hb 좌위는 Hb A와 B 대립유전자가 검출되었으며, 유전자형의 분포는 Hb AA, AB 및 BB형에서 각각 83.19, 16.39 및 0.42%이었고, Hb A와 B의 유전자빈도는 각각 0.914 및 0.086%이었다.

• IMMUNE NETWORK
• /
• 제7권3호
• /
• pp.117-123
• /
• 2007

Background: This study was designed to investigate the role of the hepatocyte growth factor (HGF) with regards to differentiation of somatic stem cells originating from the human umbilical cord blood (UCB) into hepatic lineage cells in vitro culture system. Methods: Mononuclear cells from UCB were cultured with and without HGF based on the fibroblast growth factor (FGF)-1, FGF-2, and stem cell factor. The cultured cells were confirmed by immunofluorescent staining analysis with albumin (ALB), cytokeratin-19 (CK-19), and proliferating cell nuclear antigen (PCNA) MoAb. ALB and CK-18 mRNA were also evaluated by reverse transcription-polymerase chain reaction. In order to observe changes in proliferating capacity with respect to the cultured period, CFSE with affinity to proliferating cells were tagged and later underwent flow cytometry. Results: In the HGF-treated group, cultured cells had a large oval shaped appearance with adherent, but easily detachable characteristics. In the HGF-non treated group, these cells were spindle-shaped with strong adherent characteristics. Expressions of ALB and CK-19 were evident in HGF-treated group compared to non-expression of those in to HGF-non treated group. Dual immunostaining analysis of the ALB producing cells showed presence of PCNA in their nuclei, and ALB and CK-18 mRNA were detected on the 21st day of cultured cells in the HGF-treated group. Conclusion: Our findings suggest that HGF has a pivotal role in differentiating somatic stem cells of human UCB into hepatic lineage cells in vitro.

• 한국동물학회지
• /
• 제36권2호
• /
• pp.193-199
• /
• 1993

The protein po;vmorphisms and allele frequencies of wild house rat (Rattus norvegicus) population in Korea were studied. The studied proteins and enzymes were transferrin (Tf), albumin (Alb), fumarate hvdratase (FH), phospho!loucomutase (PGM), lactate dehvdrogenase A (LDHA) and lactate dehvdrogenase B (LDHB). There were two transferrin alleles, TP and Tf in Korean wild house rat popu1ation. The Tf2 allele was found for the first time by a starch gel, and confirmed by a polvacrvlamide gel isoelectric focusing and immunoblotting. The allele frequencies of TP and TF were 0.985 and 0.015, respectively. Two common alleles fumarate hydratase, FHa and FHb were found, and frequencies of FHa and FPP were calculated to be 0.714 and 0.286, respectively. The kequenw of FH in Korean wild house rat was higher than that of Finnish and Czechoslovakian population. Alb, PGM, LDHA and LDHB are only one phenotype each and all. Therefore, these proteins seem to be monomorphic in Korean wild house rat population.

• 한국수산과학회지
• /
• 제28권2호
• /
• pp.131-136
• /
• 1995

조피볼락, 잉어 및 무지개송어의 혈청을 상온$(15^{\circ}C)$ 저온$(4^{\circ}C)$, 냉동$(-20{\circ}C)$으로 보존하면서 총단백질(TP), 알부민(ALB), 중성지방(TG), 콜레스테롤(CHOL), 포도당(GLC), 인(P), 나트륨(Na), AST 및 ALT의 활성 변화를 상온과 저온에서는 16일까지, 냉동에서는 30일까지 조사하였다. 어종간에 다소의 차이가 있기는 하지만 대체로 단백질 성분인 TP, ALB과 당 성분인 GLC, 무기질인 P, Na의 농도는 안정하였고, 지방 성분인 TG와 CHOL, AST, ALT의 활성은 불안정하였다 그리고 온도에 따른 혈액 성분의 안정성은 상온에서보다는 저온과 냉동에서 대체로 안정하지만, 성분에 따라서는 냉동하여도 매우 불안정한 것이 있다

• 대한수의학회지
• /
• 제29권3호
• /
• pp.263-274
• /
• 1989

The present study was conducted ill. order to get the normal blood chemical values of Korean Jindo dogs. Blood samples were taken from 160(♂34, ♀126) healthy Jindo dogs in Jindo area. The results obtained in this study were summarized as follows: 1. The mean values of serum total protein(TP), albumin(Alb) and globulin(Glb) content, cholesterol(Chole), magnesium(Mg), calcium(Ca), inorganic phosphate(P), potassium(K), sodium(Na) and chloride(Cl) concentration in the group of less than one year old were 6.64(♂6.62, ♀6.64), 3.63(♂3.57, ♀3.65) and 3.00(♂3.05, ♀2.99)g/100ml, 170.97(♂166.46, ♀172.68)mg/100ml, 1.45(♂1.43, ♀1.46), 5.76(♂5.62, ♀5.81), 4.80(♂4.95, ♀4.75), 4.84(♂4.72, ♀4.89), 148.93(♂148.79, ♀148.98) and 110.22(♂110.42, ♀110.14)mEq/L, respectively, whereas the TP, Alb and Glb content, Chole Mg, Ca, P, K, Na and Cl concentration in the group of one year old and more were 6.88(♂6.84, ♀6.89), 3.65(♂3.63, ♀3.66) and 3.23(♂3.21, ♀3.23)g/100ml, 167.48(♂173.80, ♀166.48)mg/100ml, 1.40(♂1.36, ♀1.40), 5.69(♂5.53, ♀5.71), 4.62(♂4.73, ♀4.60), 4.88(♂4.90, ♀4.87), 149.86(♂149.60, ♀149.90) and 110.03(♂110.70, ♀109.92)mEq/L, respectively. The ratios of mean serum albumin to globulin(A/G), calcium to inorganic phosphate(Ca/P) and sodium to potassium(Na/K) in the group of less than one year old were 1.21(♂1.17, ♀1.22), 1.20(♂1.14, ♀1.22) and 30.77(♂31.52, ♀30.47), respectively, whereas the A/G, Ca/P and Na/K in the group of one year old and more were 1.13(♂1.13, ♀1.13), 1.23(♂1.17, ♀1.24) and 30.71(♂30.53, ♀30.78), respectively. 2. The mean values of Alb content, Mg, Ca and K concentration, A/G and Ca/P ratio appeared to be higher in the female than in the male, whereas the reverse was the case with P concentration. No differences were found between male and female in the TP and Glb content, Chole, Na and Cl concentration and Na/K ratio. 3. The mean values of Glb content appeared to be higher in the group less than one year old than in the group of one year old and more, whereas the reverse was the case with A/G ratio. The mean values of TP and Alb content and Ca/P ratio in the age groups less than one year tended to increase with aging, whereas the reverse was the case with Ca and P concentration. No differences were found between age groups in the Mg, K, Na and Cl concentration and Na/K ratio.

• 한국어병학회지
• /
• 제24권3호
• /
• pp.247-254
• /
• 2011

이 연구는 FUJI DRI-CHEM 3000의 건식 자동생화학 분석기를 이용하여 조사한 어류 혈액검사 항목 가운데, 적용할 수 있는 분석항목의 활용도를 평가함과 동시에 어류 혈액학적 건강진단에 필요한 분석항목의 참고자료를 얻고자 하였다. 국립수산과학원 내 수면양식연구센터에서 사육 중인 육안적으로 보아 건강한 무지개송어 (Onchorhynchus mykiss), 이스라엘잉어 (Cyprinus carpio), 틸라피아 (Oreochromis niloticus) 및 뱀장어 (Anguilla japonica) 총 200마리를 대상으로 실시하였다. Hemoglobin (Hb), 총단백 (TP), 알부민 (ALB), 알칼리성포스파타제 (ALP), 요소질소 (BUN), 젖산탈수소효소 (LDH), 중성지방 (TG), 총콜레스테롤 (TCHO), 크레아티닌 (CRE), aspartate aminotransferase (AST), alanine aminotransferase (ALT), 포도당 (GLU) 은 FUJI DRI-CHEM 3000 기종을 이용하였다. 그 결과, 이 장비의 검출범위 (detectable range)에서 최고값 (maximum) 과 최저값 (minimum) 을 벗어난 분석항목들 (ALT, TG, LDH, ALB, TCHO, AST, ALP) 이 출현하였다. 이러한 검출범위를 초과한 평균비율을 살펴보면, ALT 및 TG가 67%~61.5%, LDH, ALB 및 TCHO는 36%~17%, AST 및 ALP는 5.5%~0.5%로 나타났다. 반면에, BUN, CRE, GLU, Hb 및 TP의 분석값은 모두 검출범위의 영역 안에서 벗어나지 않았다.

• 농업과학연구
• /
• 제43권5호
• /
• pp.818-827
• /
• 2016

This study hypothesized that dietary feed additive containing probiotics alter either immune-related serum substances or serum metabolites in Hanwoo heifers. A probiotic treatment was given at 0.5% top-dressing of concentrate diet for 6 months. The change of immunological indicators in the blood was analyzed under LPS (Lipopolysaccharide) challenge. One day before administration of LPS, all heifers were fitted with an indwelling jugular vein catheter for serial blood collections. Both a serum tube and an EDTA-coated tube were collected at 30-min intervals from - 2 to 8 hours relative to the LPS challenge at time 0 ($1{\mu}g/kg$ of BW). Serum was used for analyzing albumin (ALB), glucose (GLU), total protein (TP), triglycerides (TG), phosphorus (IP), and non-esterified fatty acids (NEFA). Plasma was used for analyzing white blood cell (WBC), red blood cell (RBC), platelet (PLT) and inflammation-related factors (NE, LY, MO, EO, BA, Hb, HCT, MCV, MCH, MCHC, RDW, MPV). There were significant differences in ALB, GLU, TG, IP, and NEFA concentration with the passage of hours post challenge (p < 0.05). The level of ALB, GLU, TG, and IP showed significant difference (p < 0.05) between treatments. However, none of the data showed interaction between time and treatments (p > 0.05). The level of WBC, EO, LY, and MO were reduced after LPS challenge (p > 0.05). In conclusion, LPS challenge after dietary supplementation of probiotics changed the levels of both serum metabolites and inflammation-related factors. The increase of GLU and TG indicated a probiotics-positive response under LPS challenge (p < 0.05).

• 생물정신의학
• /
• 제2권1호
• /
• pp.70-76
• /
• 1995

A few researchers have reported that major depression may be associated with higher levels of positive acute phase proteins(APPs), such as haptoglobin(Hp), ${\alpha}1$-antitrypsin(${\alpha}1AT$), ceruloplasmin(Cp) and lower levels of negative APPs(visceral proteins), such as albumin(Alb) and transferrin(Tf). Elevated levels of positive APPs and a drop in negative APPs constitute important indicators of immune activation. This study was designed to investigate whether altered serum concentrations of positive APPs and of negative APPs reflect the state of depression. Twenty patients who fulfilled DSM-III-R criteria for major depressive disorder and for dysthymic disorder and twelve normal healthy controls were included. The authors measured positive APPs(Hp, ${\alpha}1AT$, Cp) and negative APPs(Alb, Tf) using rate nephelometry and bromcresol green method. 1) There were significant increases of ${\alpha}1AT$, Cp in major depressed patients as compared with normal controls. Trends towards higher Hp and lower Alb, Tf in major depressed patients were observed. 2) No significant difference of APPs concentrations between dysthymic patients and normal controls was found. 3) Severity of depression(HDRS, BDI score) was related to Hp, Cp, ${\alpha}1AT$ value positively. Our findings are partially compatible with the hypothesis that major depression may be accompanied by acute phase response with higher levels of positive APPs and lower levels of negative APPs.