• Journal of Society of Preventive Korean Medicine
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• v.13 no.2
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• pp.51-64
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• 2009

Objective : This research was aimed to investigate the anti-tumor effect, safety, mechanism and metabolizing enzyme of Agrimonia pilosa LEDEB(APL) in female C57B/L mouse. Methods : At first, to evaluate the anti-tumor activity of APL, we divided into four groups, normal, control, APL100(100mg/kg), APL150(150mg/kg). LLC obtained American Type Culture Collection was used. LLC had been inoculated to induce tumor. To measure the anti-tumor effect of APL, we calibrate tumor size and weight. To study for mechanism of anti-tumor in APL, we used western blotting and to know metabolizing enzyme in APL we used to real-time PCR. Results : APL100, APL150 inhibited tumor growth after medicine injected. APL did not only induced caspase-dependent apoptosis in LLC-bearing mouse tumor. In APL100, it were decreased 72% in CYP3A11. In APL150, it were decreased 62%, 75% in CYP3A11 and MRP1a respectively. Conclusion : These results suggests that APL has some anti-tumor effects in female C57B/L mouse tumor. APL should be careful use with other drugs related with CYP3A11 or MRP1a.

• The Korea Journal of Herbology
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• v.31 no.2
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• pp.63-69
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• 2016

Objectives : The aerial parts of Agrimonia pilosa Ledeb (Agrimoniae Herba; AH) has been traditionally used as a Korean medicine to treatment of abdominal pain, sore throat, headaches, bloody discharge, parasitic infections and eczema. In this study, we investigated the effect of AH ethanol extract on lipopolysaccharide (LPS)-induced inflammation in RAW264.7 macrophage cells.Methods : AH was extracted by 30% ethanol (AH-E). Raw264.7 cells were treated with AH-E extract at different concentrations for 30 min and then stimulated with LPS (1㎍/㎖) or without for indicated times. Cell viability was measured by MTT assay, and nitric oxide (NO) production was measured by Griess assay. The expression of inflammatory mediators, iNOS and COX-2 and inflammatory cytokines, TNF-α, IL-1β, and IL-6 was detected by RT-PCR, and the phosphorylation of ERK1/2, p38 and JNK MAP kinases (MAPKs) was analyzed by Western blot. Also, the expression of NF-κB in nuclear and cytosol was detected by Western blot.Results : AH-E extract significantly decreased LPS-induced NO production in RAW264.7 cells. AH-E extract inhibited the mRNA expression of iNOS, COX-2, TNF-α, IL-1β, and IL-6 in LPS-stimulated cells with a dose-dependent manner. In addition, the phosphorylation of ERK, p38 and JNK MAPKs was also inhibited by AH-E extract. AP-E extract inhibited the nuclear translocation of NF-κB in LPS-stimulated cells.Conclusions : Our results suggest that AH-E extract has an anti-inflammatory activity in macrophages-mediated inflammation.

• Korean Journal of Medicinal Crop Science
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• v.10 no.3
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• pp.167-170
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• 2002

Agromonia pilosa L. has been used as a medicinal plant in traditional folk remedy, and it shows increasing tendency at various sections such as medicine-making material, functional food, and agricultural chemicals using plant or extract. Dosage effect of extract from Agrimonia pilosa L. plant on rat performance experiments were summarized as follows : Body weight was increased with 0.02% dosage treated-rat by 5% as compared to non-treated one, however, decreasing tendency was observed with 0.04% extract of Agrimonia pilosa L. plant fed rat to control Considering feed efficiency was similar result between extract dosage with 0.02% and non-treated rat. The number of leucocyte was increased by dosage-treated level except 0.02% dosage-treated rat. Number of erythrocyte was increased with 0.06% extract dosage-treated rat by 20% as compared to non-treated one.

• Korean Journal of Medicinal Crop Science
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• v.15 no.2
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• pp.89-94
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• 2007

The effects of angiogenesis inhibitor from the extract libraries of Korean and Chinese medicinal plants were investigated using a protein microarray chip. Protein chip was constructed by immobilization of integrin ${\alpha}_5{\beta}_1$ on protein chip base plates and employed far screening active extracts that inhibit the integrin-fibronectin interaction from the extract libraries. The 100 extracts of medicinal plants were obtained from extract bank of National Institute of Crop Science, RDA. The 14 extracts among 100 extract libraries were shown efficient inhibition activity for the interaction between integrin-fibronectin. The medicinal plants of 14 extracts were Vitex negundo var. incisa (Lam.) C.B. Clarke, Epimedium koreanum Nakai, Cedrela sinensis A. Juss, Ipomea aquatica Forsk, Schisandra chinensis Baill, Pulsatilla koreana Nakai, Paeonia lactiflora Pall. var.hortensis Makino, Oenothera odorata, Allium chinense, Allium victorialis var. platyphyllum MAKINO, Polygonatum odoratum Druce var. pluriflorum Ohwi, Hosta lancifolia, Agrimonia pilosa L. var. japonica Nakai and Potentilla chinensis SER. The Paeonia lactiflora, Oenothera, and Agrimonia pilosa from these 14 extracts libraries were shown strong inhibition activity of integrin ${\alpha}_5{\beta}_1$.

• Korean Journal of Pharmacognosy
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• v.37 no.1 s.144
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• pp.28-32
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• 2006

Hepatoprotective effects of an aqueous extract prepared from the aerial part of Agrimonia eupatoria L., a species of agrimmony, were investigated in experimental liver-damaged models. To investigate hepatoprotective effects, the agrimmony extract were fed orally to experimental animals. Thereafter a single dose of hepatotoxin, carbon tetrachloride $(CCl_4)$ or D-galac-tosamine was orally administrated. Chronic liver damage was induced by oral administration of $CCl_4$ for 2 weeks (1 time/day). Hepatoprotective effects were monitored by estimating serum AST and ALT levels. The results showed that the agrimmony extract significantly reduced AST and ALT levels compared with those of control group in both acute and chronic animal models. It was concluded that the agrimmony extract have hepatoprotective effects against rat liver injury induced by $CCl_4$ or D-galactosamine.

• Korean Journal of Plant Resources
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• v.15 no.3
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• pp.250-259
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• 2002

Agromonia pilosa Ledeb. has been used as a medicinal plant in traditional folk remedy. There are few reports on classification, physiology, ecology and morphological studies of Agromonia pilosa L. in Korea. Therefore, advanced approaches on study and development with this plant would be done urgently. Present stndy was carried out to gain basic information on genetic resources and variation with collected domestic landraces through RAPD analysis in Agromonia pilosa L. Forty two collections of Agromonia pilosa L. from nation-wide area including USA one were analyzed by RAPD test. Molecular marker size by amplified DNA band pattern ranged from 300 to 2,100bp. Among the collection, two landraces of Hadong and Cheonghak-dong showed close relation in genetic similarity. Minimum and maximum value by matrix of 1-F among 26 collected landraces were figured out as 0.365 and 0.827 showing mean value for 0.624, respectively. Those landraces were classified into two groups with cluster analysis by Nei and Li's formula from RAPD-analyzed values, and considerable genetic differences were recognized between two groups.

• Journal of Food Hygiene and Safety
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• v.11 no.3
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• pp.209-214
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• 1996

Irradiated and non-irradiated Korean medicinal herbs were extracted by water and 70% ethanol. Antimicrobial activity of these extracts were invesigated against selected food hygiene microoganisms. The ethanol extracts of the non-irradiated Agrimonia pilosa ledebour japonica Nakai, Curcuma longa Linne and Angelica gigas Nakai were completely inhibited on four species of bacteria, such as Vibrio parahaemolyticus, Clostridium perfringenes, Bacillus subtilis and Staphylococcus aureus. Also, above four strains did not have antibacterial activity in the water-ethanol mixtures. Futhermore, the ehtanol extracts of the non-irradiated Agimonia pilosa ledebour Japonica Nakai, Curcuma Iedoaria Roscoe, Curcuma longa Linne and Scutellaria baikalensis George were shown inhibitory effects against Aspergillus flavus and Pen icillium islandicum. And the water extract of Scutellaria baikalensis George was the same effect to these molds. Essentially the same results were observed when samples irradiated at a dose of 10 kGy.

• Korean Journal of Pharmacognosy
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• v.33 no.2 s.129
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• pp.144-150
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• 2002

The methanol extracts of 132 herbal medicines were screened for the inhibitory activity against heparinase enzyme from Flavobacterium heparinum. Eleven medicinal plants, Amomum xanthiodides, Agrimonia pilosa, Paeonia lactiflora, Rubia cordifolia, Sanguisorba officinalis, Torrega grandis, Morus alba, Gleditsia sinensis, Crataegus pinnatifida, Cornus officinalis, Paeonia japonica showed potent inhibition on heparinase activity. The active substituents of those herbal medicine could be extracted into butanol fraction and the inhibitory compounds of Morus alba are now isolating.

• Plant Resources
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• v.4 no.2
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• pp.85-91
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• 2001

Many plant species are used in China in tradtional medicine for the prevention and treatment of cancer. This paper presents some of these species with defals on other pharmacologic evaluation and its tradional Chinese meditional uses. The known bioaktivities and some chemical constituents of each of the species given. Information on bioactivities of each species resulting from tests on experimental animals are given. Many of the known chemical constituents of each species are given. Various species of plants elective for various types. In this meeting I will present on some antitumor of the plants which are as follows ; (1) Akebia trifoliata(Thunb.)Koidz. ((2) Panax notoginseng (Burk.) F, H, Chen ., (3)Ziziphus jujuba Mill., (4)coriotus versicolor(Fr.) Quel., (5) Trich osanthes kirilowii Maxim., (6) Ficus carica Linnaeus., (7) Acanthopanax senticosus(Rupr. et Maxim.)Harms.,(5) Hibiscus mutabilis Linnaeus.,(9) Arctium lappa Linnaeus., (11) Agrimonia pilose Ledebour and (12) Hedyotis diffusa Willd.

• Proceedings of the Korean Society of Crop Science Conference
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• 2006.04a
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• pp.582-583
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• 2006