• Journal of Microbiology and Biotechnology
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• v.23 no.11
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• pp.1617-1626
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• 2013

The Bacterial community structure and its complexity of the enrichment culture during the isolation and screening of imidacloprid-degrading strain were studied using denaturating gradient gel electrophoresis analysis. The dominant bacteria in the original tea rhizosphere soil were uncultured bacteria, Rhizobium sp., Sinorhizobium, Ochrobactrum sp., Alcaligenes, Bacillus sp., Bacterium, Klebsiella sp., and Ensifer adhaerens. The bacterial community structure was altered extensively and its complexity reduced during the enrichment process, and four culturable bacteria, Ochrobactrum sp., Rhizobium sp., Geobacillus stearothermophilus, and Alcaligenes faecalis, remained in the final enrichment. Only one indigenous strain, BCL-1, with imidacloprid-degrading potential, was isolated from the sixth enrichment culture. This isolate was a gram-negative rod-shaped bacterium and identified as the genus Ochrobactrum based on its morphological, physiological, and biochemical properties and its 16S rRNA gene sequence. The degradation test showed that approximately 67.67% of the imidacloprid (50 mg/l) was degraded within 48 h by strain BCL-1. The optimum conditions for degradation were a pH of 8 and $30^{\circ}C$. The simulation of imidacloprid bioremediation by strain BCL-1 in soil demonstrated that the best performance in situ (tea soil) resulted in the degradation of 92.44% of the imidacloprid (100 mg/g) within 20 days, which was better than those observed in the ex situ simulations that were 64.66% (cabbage soil), 41.15% (potato soil), and 54.15% (tomato soil).

• Journal of Information Processing Systems
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• v.16 no.6
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• pp.1424-1436
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• 2020

Although huge progress has been made in current image segmentation work, there are still no efficient segmentation strategies for tree image which is taken from natural environment and contains complex background. To improve those problems, we propose a method for tree image segmentation combining adaptive mean shifting with image abstraction. Our approach perform better than others because it focuses mainly on the background of image and characteristics of the tree itself. First, we abstract the original tree image using bilateral filtering and image pyramid from multiple perspectives, which can reduce the influence of the background and tree canopy gaps on clustering. Spatial location and gray scale features are obtained by step detection and the insertion rule method, respectively. Bandwidths calculated by spatial location and gray scale features are then used to determine the size of the Gaussian kernel function and in the mean shift clustering. Furthermore, the flood fill method is employed to fill the results of clustering and highlight the region of interest. To prove the effectiveness of tree image abstractions on image clustering, we compared different abstraction levels and achieved the optimal clustering results. For our algorithm, the average segmentation accuracy (SA), over-segmentation rate (OR), and under-segmentation rate (UR) of the crown are 91.21%, 3.54%, and 9.85%, respectively. The average values of the trunk are 92.78%, 8.16%, and 7.93%, respectively. Comparing the results of our method experimentally with other popular tree image segmentation methods, our segmentation method get rid of human interaction and shows higher SA. Meanwhile, this work shows a promising application prospect on visual reconstruction and factors measurement of tree.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.6
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• pp.886-890
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• 1999

From previous studies, there is a strong possibility in buffaloes that the marked increase in blood volume (BV) under hot conditions contributes to heat transportation from the rectum to the skin. The present study was done to clarify changes with environmental temperature on water-shift between blood and extracellular fluid (ECF), heat distribution between the rectum and the skin, and blood flow rates (BFR) at the hind legs (reflecting the skin surface). Four buffaloes and four Friesian cows were successively exposed to three different temperatures of $20^{\circ}C$, $30^{\circ}C$ and $35^{\circ}C$. BV and ECF volume were measured with Evans' blue and sodium-thiocyanate dilution methods, respectively. Rectal and subcutaneous (as the skin) temperatures were measured by copper-constantan thermocouples. BFR were measured by a supersonic blood flow meter. With an increase in environmental temperature, skin temperature in buffaloes increased significantly than cows, but rectal temperature was not significantly different between two species. BV, especially plasma compartment, increased significantly in only buffaloes, while ECF volume did not change in both species. BFR increased significantly in buffaloes, but not in cows. From these results, the increased of BV may be caused by water flowing from ECF compartment. The water-shift may induce the increase of BFR and skin temperature. It is suggested in the present study that internal changes of blood compartment in buffaloes contribute to transfer of heat to the skin surface.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.7
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• pp.998-1001
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• 2002

Saltwort (Salicornia herbacea L.), a kind of halophytes in Japan, is under investigation as a feed source for livestock as well as potential oilseed crop in salt marshes and salt fields. The present experiment was undertaken to analyze the nutritive value of saltwort as feed for ruminants. To determine the apparent digestibility and nutritive value of saltwort, five Japanese native goats were fed the diets consisting of alfalfa hay cubes with 15 or 20% (n=3 and n=2, respectively) inclusion levels of saltwort in the total diet on a DM basis. All the animals were randomly offered alfalfa hay cubes as a base diet or a mixed diet of alfalfa hay cubes and saltwort at maintenance level, thereafter, alternative feed (a base or mixed diet) was offered to the experimental animals (the incomplete crossover design). Analysis of the chemical composition of saltwort showed that the plant contained high levels of total ash (40.2% DM), sodium (12.7% DM) and chlorine (19.7% DM), and relatively high levels of CP (11.7% DM) and NDF (40.4% DM). Contents of TDN and digestible CP (DCP), DE and ME of saltwort were 33.5% DM, 8.4% DM, 7.4MJ/DM kg and 5.0MJ/DM kg, respectively. These results indicate that saltwort is rich in DCP and minerals (mainly sodium chloride), but poor in energy, suggesting that saltwort could be used as a mineral or CP supplement for ruminants raised around salinized areas in which high quality feed may not be available.

• Korean Journal of Veterinary Research
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• v.45 no.2
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• pp.233-238
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• 2005

We examined the teats of slaughtered cattles to investigate the prevalence of papilloma and to detect bovine papilloma virus (BPV) from those samples. Among 880 heads, 432 (49.0%) was Holstein and 448 (51.0%) was Korean native cattle. Grossly, out of 432 heads (Holstein), 263 (60.8%) had papilloma lesions on teats. However, out of 448 heads (Korean native cattle), only 33 (7.4%) had papilloma lesions on teats. Immunohistochemically, BPV antigen was detected in 35 heads (22.9%) of 153 (Holstein) which had papilloma lesions. But, in Korean native cattle, BPV antigen was detected in 1 head (3.8%) of 26. Electronmicroscopically, in Holstein papilloma cases, BPV particles was detected in 31 heads (39.2%) of 79. This study revealed that papilloma was very prevalent in holstein teats, Korea and the prevalence of papilloma in Holstein was 8 times higher than that of Korean native cattle. Moreover, it was needed to consider gross and histopathological lesions to confirm BPV infection on teats because the sensitivity of immunohistochemistry and electronmicroscopy was not high.

• Journal of Biosystems Engineering
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• v.40 no.2
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• pp.153-158
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• 2015

Purpose: The research presented in this paper applied the chemometric analysis to the near-infrared spectral data from line-scanned hyperspectral images of beef and fish meals in animal feeds. The chemometric statistical models were developed to distinguish beef meals from fish ones. Methods: The meal samples of 40 fish meals and 15 beef meals were line-scanned to obtain hyperspectral images. The spectral data were retrieved from each of 3600 pixels in the Region of Interest (ROI) of every sample image. The wavebands spanning 969 nm to 1551 nm (across 176 spectral bands) were selected for chemometric analysis. The partial least squares regression (PLSR) and the principal component analysis (PCA) methods of the chemometric analysis were applied to the model development. The purpose of the models was to correctly classify as many beef pixels as possible while misclassified fish pixels in an acceptable amount. Results: The results showed that the success classification rates were 97.9% for beef samples and 99.4% for fish samples by the PLSR model, and 85.1% for beef samples and 88.2% for fish samples by the PCA model. Conclusion: The chemometric analysis-based PLSR and PCA models for the hyperspectral image analysis could differentiate beef meals from fish ones in animal feeds.

• Smart Structures and Systems
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• v.30 no.1
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• pp.75-88
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• 2022

Engineering structures in operation essentially belong to time-varying or nonlinear structures and the resultant response signals are usually non-stationary. For such time-varying structures, it is of great importance to extract time-dependent dynamic parameters from non-stationary response signals, which benefits structural health monitoring, safety assessment and vibration control. However, various traditional signal processing methods are unable to extract the embedded meaningful information. As a newly developed technique, variational mode decomposition (VMD) shows its superiority on signal decomposition, however, it still suffers two main problems. The foremost problem is that the number of modal components is required to be defined in advance. Another problem needs to be addressed is that VMD cannot effectively separate non-stationary signals composed of closely spaced or overlapped modes. As such, a new method named generalized adaptive variational modal decomposition (GAVMD) is proposed. In this new method, the number of component signals is adaptively estimated by an index of mean frequency, while the generalized demodulation algorithm is introduced to yield a generalized VMD that can decompose mode overlapped signals successfully. After that, synchrosqueezing wavelet transform (SWT) is applied to extract instantaneous frequencies (IFs) of the decomposed mono-component signals. To verify the validity and accuracy of the proposed method, three numerical examples and a steel cable with time-varying tension force are investigated. The results demonstrate that the proposed GAVMD method can decompose the multi-component signal with overlapped modes well and its combination with SWT enables a successful IF extraction of each individual component.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.11
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• pp.1585-1590
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• 2002

Thirty female goats of local breed (mature weight 28 kg), weighing 11 to 18 kg, were used in a growth experiment with the objectives to evaluate the effect of different levels of Gliricidia sepium leaves in a diet of Guinea grass (Panicum maximum) on feed intake, weight gain and apparent digestibility. The goats were allotted to five treatments, consisting of 5 levels, 0 (GL-0), 20 (GL-20), 30 (GL-30), 40 (GL-40) and 50% (GL-50) of Gliricidia sepium leaves in the diet based on the energy requirements of the goats. Another six female goats were used to study the digestibility of three dietary treatments, GL-0, GL-30 and GL-50, according to a double $3{\times}3$ Latin square arrangement. The crude protein (CP) content in Gliricidia was higher and the neutral detergent fibre (NDF) and acid detergent fibre (ADF) lower than in the Guinea grass. Increasing the amount of Gliricidia leaves resulted in similar intake of total dry matter (DM), organic matter (OM), NDF and ADF among treatments, but CP intake increased with increasing Gliricidia in the diet. The highest total DM intake was 3.0% of body weight in the GL-20 diet. The highest daily live weight gain was 43 g/day in the GL-30 diet. Inclusion of 30% of Gliricidia leaves improved the apparent DM, OM and CP digestibility compared to 0% or 50% inclusion. NDF and ADF digestibility declined with increasing levels of Gliricidia leaves in the diet.

• Plant Resources
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• v.5 no.1
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• pp.59-69
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• 2002

Twenty-three plants of Japanese apricot (Prunus mume) were collected from several sites around Mountains JIRI in Korea. Japanese apricots having the different morphological features were evenly distributed in the groups made from the cluster analysis, indicating no geographic distributions but artificial vegetations in Korea. Japanese apricots were, as based on the PCR-RAPD techniques, clustered into the three groups; a group (prototype) having the five white petals with the five red sepals, a group (green type) having the five white petals with the five green sepals, and a group (hybrid type) having the more than five red petals with various colored sepals. The prototype apricots showed higher toxicities than other type apricot against bacteria and production of less compounds in TLC plates. The polypetal types of Japanes apricot were related to those of p. armebiaca in the characteristics of seed (the ruggedness), but also to be closed to those of p. armebiaca in PCR-RAPD analysis. The cluster analysis of the twenty three apricots and its related species calculated from the two primers were shown to distinguish relationships of cultivars within species, or of individual plants within cultivars, but also to display the two overlapping bands resulted from PCR-RAPD technique.

• Journal of Environmental Science International
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• v.25 no.1
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• pp.127-134
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• 2016

This study is conducted to get current state of data collection and utilization of the trees damaged by pine wilt disease, and to efficiently collect and utilize of the trees damaged by the pine wilt disease and abandoned fumigation-treated trees at forecasts. The method to control pine wilt disease damaged area is mostly fumigation treatment system, and there is no collection and utilization of damaged trees because of absence of efficient collection system, lack of collection cost, and absence of policy, etc. A survey is conducted that asked about the satisfaction degree for the trees damaged by pine wilt disease collection and use divided into 6 topics. It was positively recognized that the need and problem of the trees damaged by pine wilt disease, and results and satisfaction of forestry mechanization while it was mostly negatively recognized that the policy satisfaction for prevention and collection, satisfaction of collection and use, collection and forestry mechanization, and satisfaction of foundation equipment for forestry mechanization, etc. As a result of path analysis, it is necessary to promote a high-tech forestry mechanization to improve satisfaction level of results and satisfaction of forestry mechanization for the trees damaged by pine wilt disease collection and utilization, it is also need to make an effort in various ways for improve satisfaction level of satisfaction of collection and utilization.