• Title/Summary/Keyword: Agricultural technology center

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Awareness of Agricultural Technology Center's Role and Function in Urban and Rural Complex City - Focusing on Comparison of Awareness between Agriculturalist and Nonagriculturalist - (도농복합시 농업기술센터의 역할 및 기능에 대한 인식 연구 - 농업인과 비농업인의 인식 비교를 중심으로 -)

  • Choi, Soo-Ho;Lee, Seung-Hyun;Kang, Eun-Jee;Kim, Young-Geun
    • Journal of Agricultural Extension & Community Development
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    • v.22 no.1
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    • pp.79-92
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    • 2015

  • Agricultural Technology Center is an education center to provide agriculturalists with information and technology related with agriculture. As domestic and international environment of agriculture and rural area is changing, functions and roles of Agricultural Technology Center are evolving according to stream of times. In 1995, a new urban and rural complex city was created for symbiotic relationship between city and rural area, As a result, user group of Agricultural Technology Center expanded to nonagriculturalists. Though the change of agricultural extension service is necessary to perform extended functions of rural area and to satisfy needs of new group of users, it is difficult to find enough studies on new functions and roles of Agricultural Technology Center to manage the service change. Therefore, this study is aimed to suggest new functions and roles of Agricultural Technology Center according to stream of times. This study were surveyed through questionnaire targeting users of Namyangju Agricultural Technology Center to compare user group awareness of Agricultural Technology Center in urban and rural complex city. According to this study result, while main purpose of agriculturalists' visiting at Agricultural Technology Center was participating in education programs related with agriculture. In contrast, nonagriculturalists usually visited the center for agricultural understanding, leisure activities, and children education, participating in field work programs. From the survey result of required functions of the center, it was revealed that nonagriculturalists expected urban agriculture, research for living improvement in rural area, adjustment education for returning farmer, and function of farming promotion, comparing agriculturalists. It is verified that this difference of user group awareness reflects the necessity that Agricultural Technology Center should change services and accept increasing use of nonagriculturalists with new functions and roles.

  • https://doi.org/10.12653/jecd.2015.22.1.0079 인용 PDF KSCI

Surface-Displayed IL-10 by Recombinant Lactobacillus plantarum Reduces Th1 Responses of RAW264.7 Cells Stimulated with Poly(I:C) or LPS

  • Cai, Ruopeng;Jiang, Yanlong;Yang, Wei;Yang, Wentao;Shi, Shaohua;Shi, Chunwei;Hu, Jingtao;Gu, Wei;Ye, Liping;Zhou, Fangyu;Gong, Qinglong;Han, Wenyu;Yang, Guilian;Wang, Chunfeng
    • Journal of Microbiology and Biotechnology
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    • v.26 no.2
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    • pp.421-431
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    • 2016

  • Recently, poly-γ-glutamic acid synthetase A (pgsA) has been applied to display exogenous proteins on the surface of Lactobacillus casei or Lactococcus lactis, which results in a surface-displayed component of bacteria. However, the ability of carrying genes encoded by plasmids and the expression efficiency of recombinant bacteria can be somewhat affected by the longer gene length of pgsA (1,143 bp); therefore, a truncated gene, pgsA, was generated based on the characteristics of pgsA by computational analysis. Using murine IL-10 as an exogenous gene, recombinant Lactobacillus plantarum was constructed and the capacity of the surface-displayed protein and functional differences between exogenous proteins expressed by these strains were evaluated. Surface expression of IL-10 on both recombinant bacteria with anchorins and the higher expression levels in L. plantarum-pgsA'-IL-10 were confirmed by western blot assay. Most importantly, up-regulation of IL-1β, IL-6, TNF-α, IFN-γ, and the nuclear transcription factor NF-κB p65 in RAW264.7 cells after stimulation with Poly(I:C) or LPS was exacerbated after co-culture with L. plantarum-pgsA. By contrast, IL-10 expressed by these recombinant strains could reduce these factors, and the expression of these factors was associated with recombinant strains that expressed anchorin (especially in L. plantarum-pgsA'-IL-10) and was significantly lower compared with the anchorin-free strains. These findings indicated that exogenous proteins could be successfully displayed on the surface of L. plantarum by pgsA or pgsA', and the expression of recombinant bacteria with pgsA' was superior compared with bacteria with pgsA.

  • https://doi.org/10.4014/jmb.1509.09030 인용 PDF KSCI KPUBS HTML

Reproductive technologies needed for the generation of precise gene-edited pigs in the pathways from laboratory to farm

  • Ching-Fu Tu;Shu-Hui Peng;Chin-kai Chuang;Chi-Hong Wong;Tien-Shuh Yang
    • Animal Bioscience
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    • v.36 no.2_spc
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    • pp.339-349
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    • 2023

  • Gene editing (GE) offers a new breeding technique (NBT) of sustainable value to animal agriculture. There are 3 GE working sites covering 5 feasible pathways to generate GE pigs along with the crucial intervals of GE/genotyping, microinjection/electroporation, induced pluripotent stem cells, somatic cell nuclear transfer, cryopreservation, and nonsurgical embryo transfer. The extension of NBT in the new era of pig breeding depends on the synergistic effect of GE and reproductive biotechnologies; the outcome relies not only on scientific due diligence and operational excellence but also on the feasibility of application on farms to improve sustainability.

  • https://doi.org/10.5713/ab.22.0389 인용 PDF