• The Plant Pathology Journal
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• 제38권2호
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• pp.115-130
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• 2022

Though information exists regarding the pathogenesis of the shot-hole disease (SH) in flowering cherry (FC), there has been a lack of research focusing on SH management. Therefore, here, we investigated the inhibitory activities of antagonistic bacteria against SH pathogens both in vitro and in vivo as well as their biochemical characteristics and bioactive compounds. Two biosurfactant-producing bacterial antagonists, identified as Bacillus velezensis strains JCK-1618 and JCK-1696, exhibited the best effects against the growth of both bacterial and fungal SH pathogens in vitro through their cell-free culture filtrates (CFCFs). These two strains also strongly inhibited the growth of the pathogens via the action of their antimicrobial diffusible compounds and antimicrobial volatile organic compounds (VOCs). Crude enzymes, solvent extracts, and biosurfactants of the two strains exhibited antimicrobial activities. Liquid chromatography/electrospray ionization time-of-flight mass spectrometric analysis of the partially purified active fractions revealed that the two antagonists produced three cyclic lipopeptides, including iturin A, fengycin A, and surfactin, and a polyketide, oxydifficidin. In a detached leaf assay, pre-treatment and co-treatment of FC leaves with the CFCFs led to a large reduction in the severity of the leaf spots caused by Epicoccum tobaicum and Bukholderia contaminans, respectively. In addition, the two antagonists produced indole-3-acetic acid, siderophore, and a series of hydrolytic enzymes, along with the formation of a substantial biofilm. To our knowledge, this is the first report of the antimicrobial activities of the diffusible compounds and VOCs of B. velezensis against the SH pathogens and their efficiency in the biocontrol of SH.

• Journal of Microbiology and Biotechnology
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• 제28권6호
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• pp.976-986
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• 2018

Knowledge about the gut bacterial communities associated with insects is essential to understand their roles in the physiology of the host. In the present study, the gut bacterial communities of a laboratory-reared insecticide-susceptible (IS), and a field-collected insecticide-resistant (IR) population of a major rice pest, the brown planthopper Nilaparvata lugens, were evaluated. The deep-sequencing analysis of the V3 hypervariable region of the 16S rRNA gene was performed using Illumina and the sequence data were processed using QIIME. The toxicological bioassays showed that compared with the IS population, IR population exhibited 7.9-, 6.7-, 14.8-, and 18.7-fold resistance to acephate, imidacloprid, thiamethoxam, and buprofezin, respectively. The analysis of the alpha diversity indicated a higher bacterial diversity and richness associated with the IR population. The dominant phylum in the IS population was Proteobacteria (99.86%), whereas the IR population consisted of Firmicutes (46.06%), followed by Bacteroidetes (30.8%) and Proteobacteria (15.49%). Morganella, Weissella, and Enterococcus were among the genera shared between the two populations and might form the core bacteria associated with N. lugens. The taxonomic-to-phenotypic mapping revealed the presence of ammonia oxidizers, nitrogen fixers, sulfur oxidizers and reducers, xylan degraders, and aromatic hydrocarbon degraders in the metagenome of N. lugens. Interestingly, the IR population was found to be enriched with bacteria involved in detoxification functions. The results obtained in this study provide a basis for future studies elucidating the roles of the gut bacteria in the insecticide resistance-associated symbiotic relationship and on the design of novel strategies for the management of N. lugens.

• Journal of the Korean Data and Information Science Society
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• 제26권4호
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• pp.947-956
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• 2015

본 연구는 문헌 및 현장에서 얻은 생물자원지식들의 가치를 평가하기 위해 전문가들을 대상으로 실시한 델파이 조사 (Delphi method)의 신뢰성을 평가하였다. 델파이조사는 전문가들이 다른 사람의 의견과 관계없이 독립적으로 전문성 있는 평가를 내린 다음에 다음 단계에서 다른 전문가들의 의견을 참고하며 자신의 의견을 수정하는 절차를 갖는다. 본 연구에서는 문헌에서 얻은 전통지식 100건, 현장에서 취득한 지식 100건 등, 모두 200건을 우선 선정한 후 전문가 6인을 선정하여 각 지식의 가치에 대하여 두 차례 평가하도록 하였다. 그 결과 두 차례의 평가점수는 연관성이 매우 높으면서 2차에서는 다른 전문가들의 의견을 수용해 어느 정도 자체수정이 발생하여 각 문항에 대한 평가점수의 표준편차가 줄어들었다. 본 조사의 신뢰성 (reliability)을 파악하기 위해 일반적인 신뢰도 계수인 크론바하 알파와 함께 일반화가능도 (generalizability) 계수를 구하였다. 이 두 신뢰도 분석을 통해 2차 평가 후 평가의 신뢰도가 상승하여 전문가에 의한 델파이 조사의 신뢰도가 매우 높다는 사실을 지지하였으나 일반화 가능도 분석 결과를 해석하는 과정에서 다른 결과를 유추할 수 있었다. 신뢰도계수가 증가하였음에도 불구하고 평가자간의 편차는 증가하여 신뢰도가 높아진 것은 평가가 상향되고 평균에 회귀하는 경향으로 잔차변동이 줄어서이지 평가자간의 의견수렴이 이루어진 결과로 볼 수는 없다는 사실이었다. 이러한 결과를 토대로 신뢰도 계수와 함께 평가자 간의 분산을 파악하여 델파이조사의 추가적인 단계 (round)가 필요함을 제시하였다.

• Journal of Forest and Environmental Science
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• 제28권2호
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• pp.84-96
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• 2012

A special type of indigenous knowledge on plants utilization and farming system was explored by the Garo tribe community in the North-East region of Bangladesh, which correspond to the severe dependence on homestead forest. Results show that the Garo are totally dependent on the natural resources and that the extent of their dependency is faithfully reflected in their ethno-botanical knowledge. Dependencies that the study addressed focused on various aspects of food, fruit, energy, timber and health care on plants products. Garo tribe community in Bangladesh followed agrosilvipastoral system in their homesteads. They have indigenous hunting procedure to trap the animal in the forest. A total of 9 foods, 15 fruits, 12 energy-producing and 11 timber species was found and recorded that the Garo used in their daily life. Moreover, Garo used 23 medicinal plants species and have vast indigenous knowledge about using herbal medicine in daily health care practices. The Garo women do mostly the household activities, managing homestead forest and helping agricultural field where men perform all hard working activities like ploughing, cutting trees, digging the soil, preparation of horticultural and agricultural land, hunting, etc. The overall quality of life of the Garo could be considerably upgraded if ethno-botanical issues and their own indigenous knowledge will complement with scientific knowledge. The findings of the study conclude that the conservation of the indigenous knowledge of the Garo tribe related to plants utilization can also be turned to good account in forest conservation and is an important tool in this tribal area of Bangladesh.

• Journal of Ecology and Environment
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• 제38권2호
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• pp.249-256
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• 2015

Islands are often habitats to unique species because they have different environmental conditions from the mainland and other islands. Another characteristic of islands is their limited natural resources, which has led island residents to heavily rely on traditional ecological knowledge (TEK) and use resources sustainably. The so-called "Maeul landscape" shows the interrelationship of biological species and people's use of natural resources. Shinan-gun is an administrative district located in the southwestern part of Korea that forms an archipelago with huge tidal flat areas. Since long ago, people's use of these tidal flats shows a high degree of biocultural diversity. Maeul landscapes also show humans' adaptation to the natural environment. For instance, strong winds blowing mainly from the northwest have led people in Shinan-gun to create "Ushil," a windbreak forest with stone blocks to block wind from their villages and agricultural fields. At present, the transfer of TEK to future generations is at stake due to socio-economic changes that cause island populations to shrink and age rapidly. Islands are often regarded as good destinations for tourism, but attention should also be given to sustainable development due to the environmental characteristics of islands. International organizations are making efforts to curb the threats of global environmental problems especially on small islands. Their activities are aimed at seeking solutions that stress the central role of biocultural diversity in establishing the sustainable use of natural resources on islands. Joint efforts oflocal people and government authorities to protect and conserve the Maeul landscape should be encouraged.

• 한국균학회지
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• 제50권3호
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• pp.243-248
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• 2022

In Japan and China, Podosphaera pseudofusca causes powdery mildew in Fatoua villosa. During a taxonomic revision of Korean powdery mildew fungi, isolates newly collected from F. villosa were studied. Through morphological examination and molecular phylogenetic analyses of the internal transcribed spacer region and large subunit gene of rDNA, the powdery mildew fungus was identified as Podosphaera xanthii. In this study, we propose the merging of P. pseudofusca sensu Braun into the P. xanthii complex. To the best of our knowledge, this is the first report of Podosphaera powdery mildew on F. villosa in Korea.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 추계학술대회
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• pp.74-74
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• 2018

Little millet (Panicum sumatrense) is well known for its salt and drought stress tolerance and high nutritional value, but very limited knowledge of genetic variation and genomic information is available. This study was to develop highly polymorphic EST-SSR markers based on cross-species transferability of derived SSRs from switchgrass EST databases and characterize newly developed EST - SSRs to better understand the genetic diversity of collected 37 germplasm accessions of little millet. A total of 779 primer pairs were designed from the 22,961 EST sequences of switchgrass (Pancium virgatum), of which 48 EST - SSR markers were developed based on the trials of transferability of these primers in little millet. The EST - SSR amplicons showed reproducible single band polymorphism and produced a total of 160 alleles with an average of 3.3 alleles per locus in 37 accessions of little millet. T he average values of expected and observed heterozygosities were 0.266 and 0.123, respectively. T he polymorphic information content (PIC) values were observed in range of 0.026 to 0.549 with an average of 0.240. The genetic relatedness among the little millet accessions was evaluated by neighbor-joining dendrogram, which grouped all accessions into two distinct groups. The validation thus demonstrated the utility of the switchgrass EST - SSR markers in assessing genomic relationships in little millet. T he findings from this study could be useful for designing strategies for the identification of diverse germplasm for conservation and future molecular breeding programs for little millet.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2016년도 학술발표회
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• pp.204-204
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• 2016

Thailand had set the National Water Management Strategy which covered main six areas in the next 12 years, i.e., by priority: (1) water for household, (2) water for agricultural and industrial production, (3) water for flood and drought management, (4) water for quality issue, (5) water from forest conservation and soil erosion protection, (6) water resources management. However due to the climate change impact, there is a question for all strategies is whether to complete this mission under future climate change. If the impact affects our target, we have to clarify how to mitigate or to adapt with it. Vulnerability assessment was conducted under the framework of ADB's (with the parameters of exposure, sensitivity and adaptive capacity) and the assessments were classified into groups due to their different characteristic and the framework of the National Water Management Strategy, i.e., water supply (rural and urban), water for development (agriculture and others), water disasters (floods (flash, overflow), drought, water quality). The assessments identified the parameters concerned and weight factors used for each groups via expert group discussions and by using GIS mapping technology, the vulnerability maps were produced. The maps were verified with present water situation data (floods, drought, water quality). From the analysis result of this water resources management strategy, we found that 30% of all projects face the big impacts, 40% with low impact, and 30% for no impact. It is clear that water-related agencies have to carefully take care approximately 70% of future projects to meet water resources management strategy. It is recommended that additional issues should be addressed to mitigate the impact from climate risk on water resource management of the country, i.e., water resources management under new risk based on development scenarios, relationship with area-based problems, priority definition by viewpoints of risk, vulnerability (impact and occurrence probability in past and future), water management system in emergency case and water reserve system, use of information, knowledge and technology in management, network cooperation and exchange of experiences, knowledge, technique for sustainable development with mitigation and adaptation, education and communication systems in risk, new impact, and emergency-reserve system. These issues will be described and discussed.

• 한국자원식물학회지
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• 제30권3호
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• pp.287-297
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• 2017

Little millet (Panicum sumatrense) is well known for its salt and drought stress tolerance and high nutritional value, but very limited knowledge of genetic variation and genomic information is available. In this study, a total of 779 primer pairs were designed from the 22,961 EST sequences of switchgrass (Pancium virgatum), of which 48 EST-SSR markers were developed based on the trials of transferability of these primers in little millet. The EST-SSR amplicons showed reproducible single band polymorphism and produced a total of 160 alleles with an average of 3.3 alleles per locus in 37 accessions of little millet. The average values of expected and observed heterozygosities were 0.266 and 0.123, respectively. The polymorphic information content (PIC) values were observed in range of 0.026 to 0.549 with an average of 0.240. The genetic relatedness among the little millet accessions was evaluated by neighbor-joining dendrogram, which grouped all accessions into two distinct groups. The validation thus demonstrated the utility of the switchgrass EST-SSR markers in assessing genomic relationships in little millet. The findings from this study could be useful for designing strategies for the identification of diverse germplasm for conservation and future molecular breeding programs for little millet.

• 농업과학연구
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• 제38권1호
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• pp.31-38
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• 2011

This study was conducted to build frameworks for a guideline of community forest projects with respect to ODA of Korea (Official Development Assistant) in forest fields. Community forestry is a worldwide phenomenon which began in developing countries such as Nepal, Gambia, Guinea, and Indonesia and has been gradually recognized as an effective measure for ODA in forestry areas from ODA/DAC countries and the other international donors. This study investigated types of community forest status, mechanisms of community forest project conducted by ODA/DAC countries or the other international donors, and CFM (Community Forest management) success factors. As a result of our study, there are different types of resource users, having different economic and social status, perspectives, knowledge systems, values, understandings and objectives in community based forestry. Consequently, the clear forest tenure systems enabling communities to get motivated for the projects, the most appropriate agroforestry systems in different countries, capability of community to manage forest and etc must be considered carefully when the community forest project are implemented for ODA of Korea in order to gain successful results from ODA in forestry fields.