• Asian-Australasian Journal of Animal Sciences
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• v.24 no.4
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• pp.471-478
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• 2011

The objectives were to compare the ability of various rumen microbial fractions to reduce nitrate and to assess the effect of nitrate on in vitro fermentation characteristics. Physical and chemical methods were used to differentiate the rumen microbial population into the following fractions: whole rumen fluid (WRF), protozoa (Pr), bacteria (Ba), and fungi (Fu). The three nitrogen substrate treatments were as follows: no supplemental nitrogen source, nitrate or urea, with the latter two being isonitrogenous additions. The results showed that during 24 h incubation, WRF, Pr and Ba fractions had an ability to reduce nitrate, and the rate of nitrate disappearance for the Pr fraction was similar to the WRF fraction, while the Ba fraction needed an adaptation period of 12 h before rapid nitrate disappearance. The WRF fraction had the greatest methane ($CH_4$) production and the Pr fraction had the greatest prevailing $H_2$ concentration (p<0.05). Compared to the urea treatment, nitrate diminished net gas and $CH_4$ production during incubation (p<0.05), and ammonia-N ($NH_3$-N) concentration (p<0.01). Nitrate also increased acetate, decreased propionate and decreased butyrate molar proportions (p<0.05). The Pr fraction had the highest acetate to propionate ratio (p<0.05). The Pr fraction as well as the Ba fraction appears to have an important role in nitrate reduction. Nitrate did not consistently alter total VFA concentration, but it did shift the VFA profile to higher acetate, lower propionate and lower butyrate molar proportions, consistent with less $CH_4$ production by all microbial fractions.

• Korean Journal of Soil Science and Fertilizer
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• v.35 no.4
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• pp.197-206
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• 2002

Volumetric soil water contents through a soil profile were monitored to identify the effects of tillage systems and soil physico-chemical characteristic on soil water movement from the soil profile. Water content profiles under no tillage (NT) and conventional tillage (CT) practices were compared at two commercial farms in central Illinois from 1992 through 1994, using neutron-scattering techniques in weekly intervals during each growing season. The volumetric water content of surface soil layers was affected more by tillage systems and rainfall amounts, whereas that of the subsoil layers was more strongly affected by soil types. Soil water percolated faster through Saybrook and Catlin soils than through Drummer, Flanagan, and Ipava soils because Saybrook and Catlin soils have lower clay content and water-retention capacity and higher permeability than Drummer, Flanagan, and Ipava soils. Increased soil organic matter (SOM) in Drummer, Flanagan, and Ipava soils would be attributable to the higher soil water retention than other soil types. Soil water contents in the corn root zone were consistently higher under CT plots than under NT plots.

• Journal of the Korean Wood Science and Technology
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• v.47 no.5
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• pp.587-596
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• 2019

This study aimed to determine the antimalarial effect of the Strychnos ligustrina (SLW) wood extracts and to analyze its phytochemicals. The SLW powder samples were macerated with 100% ethanol (E100), 75% ethanol (E75), 50% ethanol (E50), 25% ethanol (E25), and aqueous (A100). The extracts were analyzed by LCMS/MS, and its in-vitro antimalarial activity was tested with Plasmodium falciparum. The results showed that the extract yields of E100, E75, E50, E25, and A100 were 4.3, 5.2, 5.3, 4.7, and 3.6%, respectively. The antimalarial activities of the A100, E25, E50, and E75 extracts were classified as active with $IC_{50}$ values of 38.6, 42.6, 42.9, and $43.7{\mu}g/mL$, respectively. But, the antimalarial activity of the E100 extract was classified as slightly active with $IC_{50}$ values of $87.4{\mu}g/mL$. The dominant compounds contained in the extracts of A100, E25, E50, E75, and E100 was the alkaloid compound, namely brucine with relative concentrations of 24.96, 24.55, 21.33, 11.79, and 11.62%, respectively.

• Proceedings of the Korean Society of Applied Entomolohy Conference
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• 2005.05a
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• pp.105-105
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• 2005

• Korean Journal of Soil Science and Fertilizer
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• v.39 no.2
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• pp.109-115
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• 2006

Understanding characteristics of claypan soils has long been an issue for researchers and farmers because the high-clay subsoil has a pronounced effect on grain crop productivity. The claypan restricts water infiltration and storage within the crop root zone, but these effects are not uniform within fields. Conventional techniques of identifying claypan soil characteristics require manual probing and analysis which can be quite expensive; an expense most farmers are unwilling to pay. On the other hand, farmers would be very interested if this information could be obtained with easy-to-use field sensors. Two examples of sensors that show promise for helping in claypan soil characterization are soil profile strength sensing and bulk soil apparent electrical conductivity (ECa). Little has been reported on claypan soils relating the combined information from these two sensors with grain crop yield. The objective of this research was to identify the relationships of sensed profile soil strength and soil EC with nine years of crop yield (maize and soybean) from a claypan soil field in central Missouri. A multiple-probe (five probes on 19-cm spacing) cone penetrometer was used to measure soil strength and an electromagnetic induction sensor was used to measure soil EC at 55 grid site locations within a 4-ha research field. Crop yields were obtained using a combine equipped with a yield monitoring system. Soil strength at the 15 to 45 cm soil depth were significantly correlated to crop yield and ECa. Estimated crop yields from apparent electrical conductivity and soil strength were validated with an independent data set. Using measurements from these two sensors, standard error rates for estimating yield ranged from 9 to 16%. In conclusion, these results showed that the sensed profile soil strength and soil EC could be used as a measure of the soil productivity for grain crop production.

• International Journal of Industrial Entomology and Biomaterials
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• v.10 no.2
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• pp.101-106
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• 2005

In this study, we describe the Bombyx mandarina hemolin cDNA. A sequence analysis of cDNA revealed a single open reading frame (ORF) of 1233 nucleotides. The deduced 410 amino acid sequence of B. mandarina hemolin contains 4 imunoglobulin (Ig) C-2 type domains. B. mandarina hemolin cDNA showed the highest sequence homology to known those of B. mori. The developmental profile in terms of expression level of hemolin mRNA was determined in the absence of a bacterial challenge. Hemolin mRNA was detected only in mid-gut, but not in hemocytes, fat body, testis, and silkglands. Hemolin mRNA in mid-gut was not detected until the spinning stage of the last instar larva, however, lit dramatically increased at the beginning of spinning and gradually decreased until pupal stage.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.6
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• pp.890-895
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• 2008

The antioxidant activity of resveratrol in cholesterol-fed rats, along with its hypolipidemic effects was determined. Thirty two male Sprague-Dawley (SD) rats were randomly divided into three groups (Control, Res30 and Res70) and fed a hyperlipidemic diet for 4 weeks. Resveratrol was suspended in 0.3% carboxymethyl cellulose (CMC) solution and given to rats of the Res30 and Res70 groups once a day for 4 weeks by oral intubation at a dose of 30 and 70 mg/kg body weight, respectively. The control group received 0.3% CMC solution alone. Resveratrol significantly lowered serum lipid, hepatic cholesterol (TC) and triglyceride (TG) levels compared to the control. Excretion of bile acids was significantly enhanced by resveratrol. The overall potential of the antioxidant system was significantly enhanced by the resveratrol as plasma and hepatic thiobarbituric acid reactive substances (TBARS) levels were lowered while serum superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activities were increased in the cholesterol-fed rats. These findings suggest that resveratrol maintains an antioxidant efficacy as well as its anti-hyperlipidemic effect.

• Asian-Australasian Journal of Animal Sciences
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• v.9 no.2
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• pp.123-126
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• 1996

Sixteen Harnai males were used to evaluate the influence of varying levels of fourwing saltbush hay on feed and water intakes as well as the blood serum mineral status in a completely randomized design. The animals were grouped randomly into four, with four animals in each. The four groups were randomly allotted low, medium, high and very high levels of saltbush hay supplementation in addition to wheat straw. The animals were given fresh water at free of choice. Weekly body weight was recorded for each individual animal. Blood serum was collected for mineral contents. The experiment lasted for eight weeks. The inclusion of saltbush leaves in the diet showed a non-significant effect on the total dry matter intake. There has been a significant increase in the water intake when very high levels of saltbush were included in the ration. Lower levels showed no effect on the water intake. The animals maintained their body weight from week 1 to week 8. No treatment by weeks interactions on the potassium and sodium levels were detected. However higher levels of saltbush increased significantly the potassium and sodium contents in the serum. Calcium contents were significantly(p<0.01) lowered with the inclusion of saltbush leaves in the diet. Whereas Phosphorous contents showed an increasing(p<0.05) trend with the higher levels of saltbush. No clinical or sub-clinical toxicological symptoms were observed in the sheep with the higher mineral contents.

• Journal of Animal Science and Technology
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• v.62 no.2
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• pp.247-262
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• 2020

Birth weight and subsequent weight gain is of critical importance in the survival and performance of piglets on a commercial swine farm setting. Oropharyngeal microbiome could influence immunity, and feeding behavior thus impacting health and weight gain. We used 16S rRNA gene sequencing to profile the composition and predicted metabolic functionality of the oropharyngeal microbiota in 8 piglets (4 with a birthweight ≤ 1.0 kg and 4 with a birthweight ≥ 1.7 kg) at 11, 26, and 63 days of age. We found 9 genera that were significantly associated with average daily gain (ADG) at 11 days (false discovery rate, FDR < 0.05) and 26 days of age (FDR < 0.1), respectively. The microbial functional profile revealed several pathways associated with ADG (FDR < 0.05). Among these, pathways related to degradation of catechols showed a positive association with ADG at 11, 26, and 63 days of age, implying a potential to breakdown the host-derived catecholamines. We also noted that pathways related to the biodegradation of nucleosides and nucleotides increased with ADG during the pre-weaning phase, while those involved in their biosynthesis decreased. Our findings provide insights into the oropharyngeal microbial memberships and metabolic pathways that are involved in a piglet's weight gain. Thus, providing a basis for the development of strategies aimed at improving weight gain in pigs.

• Journal of The Korean Society of Agricultural Engineers
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• v.63 no.6
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• pp.131-140
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• 2021

It is becoming more important to utilize reclaimed lands in South Korea, due to the increasing competition for its usage among different sectors. However, the high groundwater level and poor permeability are exposing them to deterioration by salinization. Salinization is difficult to predict because the pattern changes according to various characteristics of soil and groundwater. In this study, the capillary rising time was studied by the water content profile in the soil. The prediction equation of soil salinity was developed based on simulation result of the CHEMFLO model. to enable prediction considering various soil water content and groundwater level. The two terms constituting the equation showed the coefficients of determination of 0.9816 and 0.9824, respectively. Using the prediction equation of the study, the surface salinity can be easily predicted from the initial surface salinity and the salinity of the groundwater. In the future, more precise predictions will be possible with the results of studies on the hydraulic characteristics of various reclaimed soils, changes in water content profile by seasonal and climate events.