• 환경생물
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• 제37권3호
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• pp.317-334
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• 2019

The present study was conducted to investigate the differences in managed farming practices, including low-intensive farming, duck farming, and golden apple snail farming, in a rice ecosystem by comparing terrestrial arthropod communities. A total of 75 species from 70 genera belonging to 43 families in 11 orders were identified from 9,622 collected arthropods. Araneae, Hemiptera, and Coleoptera were the richest taxa. Collembola was the most abundant, followed by Diptera, Hemiptera, and Araneae. Bray-Curtis similarity among the farming practices was very high (76.7%). The biodiversity of each farming practice showed a similar seasonality pattern. The richest species group was the predators, followed by the herbivores. The species richness and diversity of ecologically functional groups among the farming practices were not statistically significant, except for the abundance of predators in golden apple snail farming. The biodiversity seasonality of ecological functional groups in each farming practice showed similar patterns. The biomass of Araneae, Hemiptera, Coleoptera, and Diptera was greater than the other taxa, in general. The biomass of each ecological functional group showed little difference and the biomass fluctuation patterns in each farming practice were almost the same. Collectively, the community structures and biodiversity of terrestrial arthropods among the farming practices in the present study were not different. The present study may contribute to sustain rich biodiversity in irrigated rice fields and to advanced studies of food webs or energy flow structures in rice fields for ecological and sustainable agriculture.

• Animal Bioscience
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• 제34권5호
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• pp.931-940
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• 2021

Objective: Light is a significant component of housing environment in commercial poultry industry. This study was conducted to investigate whether Pekin ducks perform better under monochromatic lights than under white light with respect to their growth performance, carcass quality, eyeball development, oxidation resistance, and cecal bacterial communities. Methods: A total of 320 one-day-old male Pekin ducklings were randomly distributed into five rooms with different light treatments, white, red, yellow, green, and blue light. Each room consisted of 4 replicated pens with 16 ducklings per pen. Results: Blue light significantly decreased fat deposition by decreasing abdominal fat. Long wavelength light, such as red, green, and yellow light, considerably increased the back-to-front eyeball diameter and the red light potentially enlarged the side-to-side eyeball diameter. Besides, the blue light had adverse effects on the oxidation resistance status in terms of increasing the product malonaldehyde of lipid oxidation and decreasing the plasma concentration of total superoxide dismutase. The phyla of Firmicutes had the greatest abundance in the green and blue treatments, while Bacteroidetes in blue treatment was the least. The genus of Faecalibacterium was significantly lower under the red light. Conclusion: The high risk of cecal health status and decreased anti-oxidation activity were observed under blue light. Red, yellow, and green light might increase the risk of oversized eyeball and cecal illness. Therefore, monochromatic lights compared to white light did not show advantages on the performance of housing ducks, it turns out that the white light is the best light condition for grow-out ducks.

• Journal of Animal Science and Technology
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• 제64권6호
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• pp.1144-1172
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• 2022

Salmonella enterica serovar Typhimurium isolate HJL777 is a virulent bacterial strain in pigs. The high rate of salmonella infection are at high risk of non-typhoidal salmonella gastroenteritis development. Salmonellosis is most common in young pigs. We investigated changes in gut microbiota and biological function in piglets infected with salmonella via analysis of rectal fecal metagenome and intestinal transcriptome using 16S rRNA and RNA sequencing. We identified a decrease in Bacteroides and increase in harmful bacteria such as Spirochaetes and Proteobacteria by microbial community analysis. We predicted that reduction of Bacteroides by salmonella infection causes proliferation of salmonella and harmful bacteria that can cause an intestinal inflammatory response. Functional profiling of microbial communities in piglets with salmonella infection showed increasing lipid metabolism associated with proliferation of harmful bacteria and inflammatory responses. Transcriptome analysis identified 31 differentially expressed genes. Using gene ontology and Innate Immune Database analysis, we identified that BGN, DCN, ZFPM2 and BPI genes were involved in extracellular and immune mechanisms, specifically salmonella adhesion to host cells and inflammatory responses during infection. We confirmed alterations in gut microbiota and biological function during salmonella infection in piglets. Our findings will help prevent disease and improve productivity in the swine industry.

• 드라이브 ㆍ 컨트롤
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• 제21권2호
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• pp.53-64
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• 2024

Global population growth has resulted in an increased demand for food production. Simultaneously, aging rural communities have led to a decrease in the workforce, thereby increasing the demand for automation in agriculture. Drones are particularly useful for unmanned pest control fields. However, the current method of uniform spraying leads to environmental damage due to overuse of pesticides and drift by wind. To address this issue, it is necessary to enhance spraying performance through precise performance evaluation. Therefore, as a foundational study aimed at optimizing drone-based pest control technologies, this research evaluated water-sensitive paper (WSP) via density map estimation using convolutional neural networks (CNN) with a encoder-decoder structure. To achieve more accurate estimation, this study implemented multi-task learning, incorporating an additional classifier for image segmentation alongside the density map estimation classifier. The proposed model in this study resulted in a R-squared (R²) of 0.976 for coverage area in the evaluation data set, demonstrating satisfactory performance in evaluating WSP at various density levels. Further research is needed to improve the accuracy of spray result estimations and develop a real-time assessment technology in the field.

• Journal of Microbiology and Biotechnology
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• 제30권8호
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• pp.1169-1179
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• 2020

In this study, two soybean genotypes, i.e., aluminum-tolerant Baxi 10 (BX10) and aluminumsensitive Bendi 2 (BD2), were used as plant materials and acidic red soil was used as growth medium. The soil layers from the inside to the outside of the root are: rhizospheric soil after washing (WRH), rhizospheric soil after brushing (BRH) and rhizospheric soil at two sides (SRH), respectively. The rhizosphere bacterial communities were analyzed by high-throughput sequencing of V4 hypervariable regions of 16S rRNA gene amplicons via Illumina MiSeq. The results of alpha diversity analysis showed that the BRH and SRH of BX10 were significantly lower in community richness than that of BD2, while the WRH exhibited no significant difference between BX10 and BD2. Among the three sampling compartments of the same soybean genotype, WRH had the lowest community richness and diversity while showing the highest coverage. Beta diversity analysis results displayed no significant difference for any compartment between the two genotypes, or among the three different sampling compartments for any same soybean genotype. However, the relative abundance of major bacterial taxa, specifically nitrogen-fixing and/or aluminum-tolerant bacteria, was significantly different in the compartments of the BRH and/or SRH at phylum and genus levels, indicating genotype-dependent variations in rhizosphere bacterial communities. Strikingly, as compared with BRH and SRH, the WRH within the same genotype (BX10 or BD2) always had an enrichment effect on rhizosphere bacteria associated with nitrogen fixation.

• 한국농림기상학회지
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• 제8권3호
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• pp.132-140
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• 2006

일월산 산림식생의 종구성적 특성을 파악하기 위하여 조사구 68개소$(10m{\sim}10m)$ 설정하여 식생유형과 그에 대한 특성(현존식물상, 상재도급과 생활형분포율, 종다양도, 층위별 중요치)을 분석하였다. 식생유형은 산악건조 지식생형과 비옥적윤한 산록과 계곡부식생형으로 대별되었으며, 전자는 3개 군락(진달래군락, 굴참나무군락, 일월비비추군락), 2개 군(잣나무군, 진달래전형군), 후자는 3군락(피나무군락, 머루군락, 고광나무군락), 깨 군(당단풍군, 참반디군)으로 구분되어 총 8개 식생단위로 나타났다. 관찰된 총 252종에서 국화과가 26종, 백합과 19종, 미나리아재비과 15종, 장미과 14종 등의 순으로 높게 나타났고, 상재도급별 분포경향에서 신갈나무(IV), 대사초(III), 물푸레나무(III)가 고중상재도종으로 나타났다. 생활형 분포율은 교목성이 18.5%, 소교목성 5.7%, 관목성 14.9%, 만목성 6.6%, 화본과류 8.8%, 광역초본 42.4%, 양치식물 3.1%로 나타났으며, 종다양도는 PH유형이 $1.70{\pm}.50{\sim}1.97{\pm}0.57$로 $1.40{\pm}0.18{\sim}1.62{\pm}0.20$인 AM유형 보다 높은 값을 나타내었다. 교목층에서는 소나무, 신갈나무, 굴참나무 등의 중요치 값이 높게 나타났으며, 아교목층은 신갈나무, 관목층은 쇠물푸레, 조록싸리, 철쭉꽃, 생강나무, 진달래, 초본층은 대사초, 산거울, 큰기름새의 영향력이 높게 나타났다.

• 한국농림기상학회지
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• 제7권4호
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• pp.303-310
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• 2005

의성군 금봉산일대 산림식생의 조성 및 구조적 특성에 대하여 정성적 및 정량적으로 분석하였고, 조사구 70개소$(10m{\times}10m)$를 설정하여 식생유형과 그에 대한 특성(상재도, 상대피도, 종다양도, 종간유사도)이 분석되었다. 식생유형은 큰기름새 등이 특징짓는 산지식생형과 고로쇠나무, 고추나무, 고광나무 등이 특징짓는 저지$\cdot$계곡식생형 등 2개의 식생형으로 대별되었다. 식생단위는 4개 군락(신갈나무군락, 굴참나무군락, 떡갈나무군락, 고로쇠나무군락)으로 구분되어 총 1때 식생그룹으로 나타났다. 상재도와 상대피도는 그룹별로 다소차이는 있지만, 상대피도는 평균적으로 교목성이 $50\~65\%$를 나타내었고, 종다양성은 저지$\cdot$계곡식생유형에서 $2.06\pm0.25\~2.31\pm0.26$으로 산지식생유형보다 높은 값을 나타내었다. 층위별 주요 구성종의 종간유사성을 보면, 교목층과 아교목층에서 신갈나무와 소나무, 떡갈나무, 피나무 등이, 고로쇠나무와 층층나무, 당느릅나무 등이 유사성이 높았으며, 관목층과 초본층에서는 신갈나무와 생강나무, 쇠물푸레나무, 산거울, 대사초 등과 고로쇠나무와 고광나무, 등칡 등이 높은 값을 나타내었다.

• Journal of Microbiology and Biotechnology
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• 제16권5호
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• pp.704-715
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• 2006

Oil spill was found in 1999 from a diesel storage facility located near the top of Baekun Mountain in Uiwang City. Application of bioremediation techniques was very relevant in removing oil spills in this site, because the geological condition was not amenable for other onsite remediation techniques. For efficient bioremediation, bacterial communities of the contaminated site and the uncontaminated control site were compared using both molecular and cultivation techniques. Soil bacterial populations were observed to be stimulated to grow in the soils contaminated with diesel hydrocarbon, whereas fungal and actinomycetes populations were decreased by diesel contamination. Most of the dieseldegrading bacteria isolated from contaminated forest soils were strains of Pseudomonas, Ralstonia, and Rhodococcus species. Denaturing gradient gel electrophoresis (DGGE) analysis revealed that the profiles were different among the three contaminated sites, whereas those of the control sites were identical to each other. Analysis of 16S rDNA sequences of dominant isolates and clones showed that the bacterial community was less diverse in the oil-contaminated site than at the control site. Sequence analysis of the alkane hydroxylase genes cloned from soil microbial DNAs indicated that their diversity and distribution were different between the contaminated site and the control site. The results indicated that diesel contamination exerted a strong selection on the indigenous microbial community in the contaminated site, leading to predominance of well-adapted microorganisms in concurrence with decrease of microbial diversity.

• Journal of Microbiology and Biotechnology
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• 제26권7호
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• pp.1320-1332
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• 2016

Light hydrocarbons accumulated in subsurface soil by long-term microseepage could favor the anomalous growth of indigenous hydrocarbon-oxidizing microorganisms, which could be crucial indicators of underlying petroleum reservoirs. Here, Illumina MiSeq sequencing of the 16S rRNA gene was conducted to determine the bacterial community structures in soil samples collected from three typical oil and gas fields at different locations in China. Incubation with n-butane at the laboratory scale was performed to confirm the presence of "universal microbes" in light-hydrocarbon microseepage ecosystems. The results indicated significantly higher bacterial diversity in next-to-well samples compared with background samples at two of the three sites, which were notably different to oil-contaminated environments. Variation partitioning analysis showed that the bacterial community structures above the oil and gas fields at the scale of the present study were shaped mainly by environmental parameters, and geographic location was able to explain only 7.05% of the variation independently. The linear discriminant analysis effect size method revealed that the oil and gas fields significantly favored the growth of Mycobacterium, Flavobacterium, and Pseudomonas, as well as other related bacteria. The relative abundance of Mycobacterium and Pseudomonas increased notably after n-butane cultivation, which highlighted their potential as biomarkers of underlying oil deposits. This work contributes to a broader perspective on the bacterial community structures shaped by long-term light-hydrocarbon microseepage and proposes relatively universal indicators, providing an additional resource for the improvement of microbial prospecting of oil and gas.

• 농촌계획
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• 제15권4호
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• pp.161-172
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• 2009

This study focuses on the application of landscape planning(i.e. including rural landscape character area designation and rural landscape planning etc.). That application of landscape planning provides the probably most operable and comprehensive version of what is generally accepted by the discipline. According to the planning discipline the schemes of planning are to; Firstly, we derived the improvement of landscape and the methods of landscape characterization subsequent to analyze the present condition and grasp the landscape resources in Yesan via reviewing the current study and field survey. Secondly, in order to achieve environmentally friendly rural landscape plan and characteristic rural landscape creation, we planed the draft landscape area such as woodland, hydrology area, urbanized area, rural communities, etc. which was classified by sphere of life moreover, we planed partly outdoor facilities, road and colour suchlike. Thirdly, we tried to seek the programme of conservation and utilization for scenic and characteristic rural landscape area via rural landscape character area designation. Lastly, it is proposed that rural landscape planning lead to inhabitant participation, system improvement, subject performance for carrying out efficient rural landscape planning.