• Journal of Biosystems Engineering
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• v.25 no.4
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• pp.311-326
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• 2000

• Proceedings of the Microbiological Society of Korea Conference
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• 2009.05a
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• pp.55-56
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• 2009

• Proceedings of the Plant Resources Society of Korea Conference
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• 2001.11b
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• pp.98-98
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• 2001

• Proceedings of the Plant Resources Society of Korea Conference
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• 2001.11b
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• pp.102-102
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• 2001

• Proceedings of the Plant Resources Society of Korea Conference
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• 2001.11b
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• pp.103-103
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• 2001

• Mycobiology
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• v.40 no.2
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• pp.142-144
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• 2012

During the search for neuraminidase inhibitors from medicinal fungi, we found that the culture broth of Phellinus linteus exhibited potent inhibitory activity. Solvent partition, Sephadex LH-20 column chromatography, and high-performance liquid chromatography (HPLC) were performed for purification of two active substances from the culture broth. According to $^1H$ NMR measurements and comparison of HPLC retention times with those of authentic compounds, their chemical structures were identified as hispidin and hypholomine B. Compounds (hispidin) 1 and 2 (hypholomine B) inhibited neuraminidase, with $IC_{50}$ values of 13.1 and 0.03 ${\mu}M$, respectively.

• 한국생물공학회:학술대회논문집
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• 2005.04a
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• pp.129-130
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• 2005

• Proceedings of the Korean Society of Applied Entomolohy Conference
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• 2005.05a
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• pp.105-105
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• 2005

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2000.11b
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• pp.472-479
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• 2000

A persimmon harvesting vehicle that can be operated in hilly orchards as well as a manipulator that can be used to harvest persimmons located in remote positions in the trees were designed and developed. The vehicle could be operated with keeping balanced position in an inclined field and its working platform could be moved up and down easy to approach fruits in a remote region with the aids of a hydraulic and a electrical and electronics systems. The weight of the vehicle was 927 kg and the center of gravity was located at 427 mm to the inner side from the center of a right driving caterpillar, 607 mm to a rear axle from the center of a front axle, and 562 mm to upward from ground. The automatic level control sensor for leveling the working platform was activated within 14.5 ∼ 16.5 degrees of slope variation. The total length of the manipulator was 1.39 m and weight is 975 g. It was powered by a 12 V geared motor to detach persimmon fruits with a rotational force. The gripper was made of plastic and rubber to increase a frictional force. In a performance evaluation test, static tipping angle, dynamic tipping angle toward front side when the vehicle was moving downward, climbing angle, driving speed of the vehicle were measured or calculated. In persimmon harvesting tests 24.9% of yield was increased by hand picking with the aid of the vehicle and additional 7% of yield were increased when the manipulator was used. Therefore, 99010 of total possible yield was achievable when both of the vehicle and the manipulator were used for the manual persimmon harvesting. Increase in 22.5% of total yield was achieved with the manipulator only.

• Journal of Microbiology and Biotechnology
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• v.20 no.1
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• pp.193-201
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• 2010

A specific and rapid real-time PCR assay for detecting Ralstonia solanacearum in horticultural soil and plant tissues was developed in this study. The specific primers RSF/RSR were designed based on the upstream region of the UDP-3-O-acyl-GlcNAc deacetylase gene from R. solanacearum, and a PCR product of 159 bp was amplified specifically from 28 strains of R. solanacearum, which represent all genetically diverse AluI types and all 6 biovars, but not from any other nontarget species. The detection limit of $10^2\;CFU/g$ tomato stem and horticultural soil was achieved in this real-time PCR assay. The high sensitivity and specificity observed with field samples as well as with artificially infected samples suggested that this method might be a useful tool for detection and quantification of R. solanacearum in precise forecast and diagnosis.