• Animal Bioscience
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• v.36 no.1
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• pp.75-83
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• 2023

Objective: The objective of this study was to describe a methodological procedure to quantify the heat production (HP) partitioning in basal metabolism or fasting heat production (FHP), heat production due to physical activity (HPA), and the thermic effect of feeding (TEF) in roosters. Methods: Eighteen 54-wk-old Hy Line Brown roosters (2.916±0.15 kg) were allocated in an open-circuit chamber of respirometry for O₂ consumption (VO₂), CO₂ production (VCO₂), and physical activity (PA) measurements, under environmental comfort conditions, following the protocol: adaptation (3 d), ad libitum feeding (1 d), and fasting conditions (1 d). The Brouwer equation was used to calculate the HP from VO₂ and VCO₂. The plateau-FHP (parameter L) was estimated through the broken line model: HP = U×(R-t)×I+L; I = 1 if t<R or I = 0 if t>R; Where the broken-point (R) was assigned as the time (t) that defined the difference between a short and long fasting period, I is conditional, and U is the decreasing rate after the feed was withdrawn. The HP components description was characterized by three events: ad libitum feeding and short and long fasting periods. Linear regression was adjusted between physical activity (PA) and HP to determine the HPA and to estimate the standardized FHP (st-FHP) as the intercept of PA = 0. Results: The time when plateau-FHP was reached at 11.7 h after withdrawal feed, with a mean value of 386 kJ/kg^0.75/d, differing in 32 kJ from st-FHP (354 kJ/kg^0.75/d). The slope of HP per unit of PA was 4.52 kJ/mV. The total HP in roosters partitioned into the st-FHP, termal effect of feeding (TEF), and HPA was 56.6%, 25.7%, and 17.7%, respectively. Conclusion: The FHP represents the largest fraction of energy expenditure in roosters, followed by the TEF. Furthermore, the PA increased the variation of HP measurements.

• The Korean Journal of Mycology
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• v.17 no.2
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• pp.51-56
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• 1989

This study was carried out to obtain basic information elucidating relationship meteorological elements and yield of Tricholoma matsutake. The yields of pine mushroom in seven areas including Yangyang-gun more than 30,000 kg. Pine mushrooms were generally distributed in the red pine (Pinus densiflora) forests of East-Coast Region, Sobaek and Taebaek-Mountainous Regions, Yeongnam-Central Region and North-Central Region of Korea. Yields of pine mushroom were significant correlation with precipitation during April-October in last years, average minima temperature during July-September, rainy days during Aug. 21-Sep. 10 and precipitation during Sep. 11-30, etc. Air and soil temperature during pine mushroom yield period were 15-25 and $15-20^{\circ}C$, respectively. The first collection date of pine mushroom could be predicted by measuring the soil temperature at 5 cm depth.

• Journal of Animal Science and Technology
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• v.45 no.6
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• pp.911-916
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• 2003

The mitochondrial DNA(mtDNA) D-loop region was amplified from Duroc(Sus scrofa) by polymerase chain reaction(PCR). The oligonucleotide primer used to amplify the Sus scrofa mtDNA D-loop region was designed using tRNA-Pro and tRNA-Phe sequence in mtDNA regions highly conserved in many other animal species. There were 1,145 base pairs(bp) in the D-loop region. The middle of the region contained 10 tandem repeat of an 10-bp Sus scrofa-specific sequence, TACACGTGCG. We designed primers for PCR-mediated single stranded conformation polymorphism(SSCP) analysis that amplified a 345 bp fragment, which contained the most variable region according to our sequencing data. SSCP analysis of denatured amplification products was carried out by polyacrylamide(8%) gel electrophoresis followed by ethidium bromide staining. The SSCP analysis identified two band patterns(A and B) and comparision of these two nucleotide sequences identified 21 base substitutions. These results show that SSCP analysis of the D-loop region is useful for detecting the genetic polymorphism.

• Korean Journal of Environmental Agriculture
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• v.4 no.2
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• pp.102-107
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• 1985

A pot experiment was conducted to find out the effects of water management, slaked lime and wollastonite on growth and Cu uptake of rice at Cu added soil. The soil was adjusted to 0, 50, 100 and 200 ppm concentration of Cu. The application amount of slaked lime was the lime requirement plus 150 ㎏/l0a and wollastonite 200 ㎏/10a, respectively. The copper concentration in soil which reduced yield significantly was 133.1 ppm for submersion and 136.8 ppm for intermittent irrigation. The application of lime and wollastonite reduced Cu content in brown rice as well as increased rice yields compared to that of no lime. The copper content in plant was increased with increasing soil Cu concentration, however, reduced with submersion and application of slaked lime, and increased with increasing the ratio of Cu/Ca+Mg equivalent in soil.

• Research in Plant Disease
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• v.14 no.3
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• pp.233-237
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• 2008

Tomato yellow leaf curl virus (TYLCV), a newly reported Geminivirus from tomato, generated recently large economic losses in Korea. Development of a fast and precise genetic diagnosis technique for detecting TYLCV which Agricultural research and extension services can utilize easy and handy is very important to prevent yield losses. Virion Capture (VC)/PCR is a simple, accurate and economical genetic detection method without any works or commercial kits for the extraction of the nucleic acid from the infected plants. Primers of twenty two for detection of TYLCV were designed and tested with extracted total DNA or crude sap from tomato leaf infected with TYLCV and healthy plant. Nine primers for total DNA using conventional PCR and another 9 primers for VC/PCR were selected eventually. Primers of six having same specificity were selected from the two methods and tested with other Geminivirus, Tobacco leaf curl virus (TLCV) by VC/PCR. Finally specific primers of four were selected for detection of TYLCV using VC/PCR, and Deng (540, 541), a degenerate primer for Geminivirus reported in 1996, was also developed for VC/PCR.

• Korean Journal of Environmental Agriculture
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• v.10 no.1
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• pp.51-57
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• 1991

Field experiments were carried out to evaluate the utility of water hyacinth(Eichhornia crassipes Solm-Laub) for purifyng sewage water in paddy fields of $247m^2$(75 Pyung). A field was lined with PVC film at a depth of 30cm and sewage water for 40 days. The results obtained with the use of water hyacinth in the sewage water are summarized as follows : 1. The Sodium linear dodecyl benzene sulfonate content of sewage water on the inlet was 6.33 ppm, themiddle point was 3.50 ppm and the outlet was 1.37 ppm. 2. With the use of water hyacinth in the sewage and the distilled water, the L.A.S. content were decreased from 7.3 and 4.0 to 0, respectively. 3. Sterillization with 70% ethyl alcohol at the roots of the water hyacinth was reduced the degradation effect of L.A.S. 4. The COD and $NH_4^+-N$ content of the sewage decreased from 107 and 32.7 ppm to 32.6 and 16.2 ppm, respectively and the P, Na and Cl content also were reduced.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.4
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• pp.504-511
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• 1995

This study was conducted to investigate the superoxide dismutase (SOD) characteristics and antioxidant activity by nonenzymatic(Fe$^{2+}$/Ascorbate) and Fe$^{3+}$-ADP/NADPH method in perilla(Perilla frutescens var. japonica Hara.) and jaso(Perilla frutescens Briton var. acuta Kudo.) leaves. The characteristics were evaluated by the nitro blue tetrazolium reduction method. Perilla leaves contained three or four major SODs depending on the varieties. The inhibitor test indicated that the Perilla leaves contained two Cu /ZnSODs and one or two FeSODs, but Jaso leaves have only Cu/ZnSOD. However, no varietal differences were detected in the Cu /ZnSOD isozyme patterns. FeSODs, however, showed different varietal isozyme patterns through the different combinations of the two FeSOD isozymes. Among MeOH extractes, "mil yang 2" showed very strong antioxidant activity. Relatively large differences in the levels of SOD and antioxidant activity detected in the Perilla varietites. There was significantly different in the comparison between perilla leaves and red jaso leaves.s.etween perilla leaves and red jaso leaves.

• Journal of Forest and Environmental Science
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• v.26 no.2
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• pp.95-102
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• 2010

Jatropha curcas is an oil bearing species with multiple uses and considerable economic potential as a biofuel plant. Plant flowering and breeding characteristics are important for us to understand the reproduction of plant populations. The present study describes the floral biology and flowering phenology of J. curcas which is a prerequisite for hybridization program for genetic improvement through conventional breeding. The plant produces flowers in dichasial inflorescences. Normally, the flowers are unisexual, and male and female flowers are produced in the same inflorescence. Only a few male flowers are produced in an inflorescence, and fruits are produced only through pollination between different flowers from the same or different plants. This study includes a description of the inflorescence, flower anatomy of both male and female flowers, female : male ratio, pollen : ovule ratio, flowering phenology, pollen viability, stigma receptivity, comparison of selfing methods and a comparison of geitonogamy and xenogamy. This information may be useful in J. curcas breeding programmes.

• The Korean Journal of Mycology
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• v.10 no.3
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• pp.125-129
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• 1982

Of about 70 different mushrooms (potential ectomycorrhizal fungi) collected from forest floor of a 24-year-old Pinus rigida rigida ${\times}$ taeda stand, four species have not been reported in Korea. They are Inocybe fastigiata, Phylloporus bellus, Lactarius glaucescens, and Lactarius subvellereus. The Phylloporus bellus has been incorrectly identified in Korea as Phylloporus rhodoxanthus. Taxonomic descriptions for these four mushrooms are reported in this paper. The collections cited are deposited in the herbarium of Institute of Agricultural science O.R.D.

• Korean Journal of Medicinal Crop Science
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• v.7 no.4
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• pp.282-287
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• 1999

This study was conducted to investigate the effects of different soil texture in agronomic characteristics, root yield and quality of baloon flower (Platycodon grandiflorum A. DC.). Three kinds of soil texture, sandy loam, silt loam and clay loam, were tested for two years. Baloon flowers were seeded with 20cm row space and cultivated in the one square meter pot with 0. 6m depth. Fifty plants per pot were remained. In growth characteristics of one-year-old plants, shooting date was not significantly different according to the changes of soil texture. The ratio of stem growth was highest between July and August. In two-years-old plants, it was showed between May and June. Plants grown on clay loam showed the highest growing characteristics than those of other soil textures. Dry root weight cultivated on clay loam was 274kg/10a in one-year-old and 934kg/10a in two-years-old plants. The content of ethanol extracts from root was highest at those of sandy loam as 47.1 % in one-year-old root. The crude saponin content was not different significantly among the three kinds of soil texture.