• Title/Summary/Keyword: Agricultural R and D

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Improved Production of Ligninase and Laccase by Phanerochaete chrysosporium and Ceriporiopsis subvermispora (Phanerochaete chrysosporium과 Ceriporiopsis subvermispora 균주(菌株)의 Ligninase 및 Laccase 생산최적조건에 관한 연구(硏究))

  • Kang, An-Seok;Cha, Dong-Yeul;Kim, Kyung-Soo;Hong, In-Pyo;Croan, Suki C.;Yu, Seung-Hun
    • The Korean Journal of Mycology
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    • v.22 no.3
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    • pp.254-259
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    • 1994
  • The ever increasing demand for energy and the shortage of resources all over the world have generated interest in recycling renewable sources such as lignocelluloses which otherwise would go to waste and cause environmental pollution. Lignin is the incrustation material for cellulose and hemicellulose, therefore, cellulose and hemicellulose are not easily degraded. Recycling lignocellulosic wastes as agricultural material are benefit to everybody and everything. In order to improve ligninase and laccase production of Phanerochaete chrysosporium, BKM-F-1767 and Ceriporiopsis subvermispora, FP 90031-SP, were compared. The ligninase activity of P. chrysosporium was maximum on day 4.5 of shaking culture at 150 rpm 2.5 cm in a back and forth cycle. The laccase activity of C. subvermispora was maximum on day 5.5 for 2% malt extract+0.1% yeast extract+0.1% Tween 20+6 mM Benzyl alcohol culture medium at stationary state.

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Estimation of Gene Effects in Four Bivoltine Silkworm (Bombyx mori L.) Crosses

  • Malik, G.N.;Singh, T.P.;Rufaie, S.Z.Haque;Aijaz, M.;Dar, H.U.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.8 no.1
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    • pp.113-115
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    • 2004
  • Six generations (P$_1$, P$_2$, F$_1$, BC$_1$, BC$_2$ and F$_2$) of four bivoltine silkworm crosses (SKAU-R-1 ${\times}$ Yakwei, SKAU-R-6 ${\times}$ Yakwei, CSR$_2$ ${\times}$ CSR$_4$ and SH$_{6}$${\times}$ NB$_4$D$_2$) were evaluated in a completely randomized block design with 5 replications for each treatment. The generation mean 2 in respect of 3 metric traits (single cocoon weight, single shell weight, and shell ratio %), were subjected to Cavallis joint scaling test. Additive dominance model was found to be adequate in CSR$_2$${\times}$CSR$_4$ and SH$_{6}$${\times}$ NB$_4$D$_2$ for single cocoon weight and shell weight and SKAU-R-6 ${\times}$ Yakwei for shell ratio(%). Whereas, in rest of the crosses epistasis was evident in the traits under investigation. Magnitude of additive gene effect (d) was greater than dominance(h) in SH$_{6}$${\times}$NB$_4$D$_2$ and SKUA-R-6${\times}$Yakwei for shell ratio (%) and in CSR$_2$${\times}$ CSR$_4$ for shell weight. Thus selection for these traits in early segregating generations of these crosses would be effective for obtaining considerable genetic gain. gain.

Isolation and Identification of Antagonistic Bacteria for Biological Control of Ginger Rhizome Rot Caused by Pythium zingiberum

  • Lee, Du-Ku;Shim, Jai-Sung;Shim, Hyeong-Kwon;Lee, Yong-Hoon;Lee, Wang-Hyu
    • Plant Resources
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    • v.2 no.2
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    • pp.81-87
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    • 1999
  • Sixteen isolates showing relatively strong antagonicity against the ginger rhizome rot pathogen, Pythium zingiberum, were selected among the 155 isolates from ginger rhizome surfaces and rhizospheres of ginger cultivation fields in Wanju, Chonbuk. The isolate, 'HB 26-5'showing the strongest antagonicity was finally selected by testing duration of inhibition effect and pathogenicity to ginger. The isolated antagonistic microorganism, 'HB 26-5' was rod shape, gram positive and formed endospore. The isolate produced acids utilizing glucose, arabinose, xylose and mannitol, and acetoin at VP test, and grew anaerobically. Temperature range for growth was from 10 to 4$0^{\circ}C$ . Reaction to catalase and gelatin, hydrolysis were positive, and casein hydrolysis and indol production were negative. Based on the mycological characters and the fatty acid composition, it was identified as Bacillus polymyxa. The pathogenicity test of isolated Bacillus polymyxa 'HB 26-5'on 22 crop cultivars resulted that only the lettuce was influenced in germination, and the others were not affected.

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Chemical Components of Paecilomyces tenuipes (Peck) Samson

  • Hong, In-Pyo;Nam, Sung-Hee;Sung, Gyoo-Byung;Chung, In-Mo;Hur, Hyeon;Lee, Min-Woong;Kim, Mi-Kyung;Guo, Shun-Xing
    • Mycobiology
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    • v.35 no.4
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    • pp.215-218
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    • 2007
  • The caterpillar-shaped Chinese medicinal mushroom (DongChongXiaCao) looks like a worm in the winter and like a grass in the summer. The fruiting body has been regarded as popular folk or effective medicines used to treat human diseases such as asthma, bronchial and lung inflammation, and kidney disease. The fruiting bodies of Paecilomyces tenuipes that formed on the living silkworm (Bombyx mon) host were used in this examination. This study was carried out to investigate the proximate composition, soluble sugar, amino acid and fatty acid profiles, and contents of the bioactive ingredient including adenosine and D-mannitol in the fruiting-bodies. The moisture content was 57.56%. Soluble sugars found were glycerol, glucose, mannitol and sucrose, and the contents exceeded $24\;mgg^{-1}dry$ weight. Total free amino acid content was $17.09\;mgg^{-1}dry$ weight. Arginine, glycine, proline and tyrosine were main amino acids. The content of oleic acid in fatty acids was high. Adenosine was more abundant in fruiting bodies than corpus.

Isolation and HPLC Analysis of Timosaponin A III from Rhizomes of Anemarrhena asphodeloides $B_{UNGE}$ (지모의 유효성분 분리 및 HPLC 정량 분석)

  • Kim, Geum-Soog;Park, Chang-Kie;Seong, Jae-Duck;Kim, Hyun-Tae;Han, Sang-Ik;Kwack, Yong-Ho
    • Korean Journal of Medicinal Crop Science
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    • v.7 no.1
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    • pp.45-50
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    • 1999
  • Timosaponin A III, an active and major compound, was isolated from rhizomes of Anemarrhena asphodeloides. The quantitative analysis of timosaponin AIII was performed by a high performance liquid chromatographic(HPLC) method using ELSD and the useful extraction method for HPLC analysis was examined as well. This HPLC method can be utilized as the standard analytical method for the evaluation of the quality of Anemarrhena rhizoma in the steps of breeding and cultivation. Additionally, the HPLC analysis method can be useful for the evaluation of the quality of Anemarrhena rhizoma sold as a traditional medicine in current markets.

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Evanescent Wave-Based Fiber Bragg Grating Biosensors

  • Lee, Sang-Mae;Kim, Deug;Dagenais, Mario;Chryssis, Athanasios N.;Saini, Simarjeet Singh;Yi, Hyunmin;Bentley, William E.
    • Proceedings of the Optical Society of Korea Conference
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    • 2006.07a
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    • pp.399-400
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    • 2006
  • Etched fiber Bragg grating, Sensitivity of fiber-Bragg-grating sensors to index of surrounding, Hybridization of DNA

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