• Journal of Microbiology and Biotechnology
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• v.11 no.6
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• pp.1002-1010
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• 2001

The D-xylose operon in Escherichia coli is known to be regulated by a transcriptional activator protein, XyIR, which is responsible for the expression of both xylAB and xylFGH gene clusters. The XyIR was purified to homogeneity by using the maltose binding protein fusion expression and purification systems involving two chromatography steps. The purified XyIR protein was composed of two subunits of 45 kDa, which was determined by both sodium dodecyl sulfate polyacrylamide gel electrophoresis and gel filtration. The purified XyIR was specifically bounded to the xylA promoter, regardless of adding xylose to the reaction mixture, but binding of XylR was specifically bounded to the xylA promoter, regardless of adding xylose to the reaction mixture, but binding of XylR to the xylA promoter was enhanced by adding xylose. The enhanced binding ability of XyIR in the presence of xylose was not diminished by adding glucose. The presumed XyIR binding site is located between 120 bp to 100 bp upstream the xylA initiation codon.

• Korean Journal of Environmental Agriculture
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• v.38 no.3
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• pp.124-132
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• 2019

BACKGROUND: Silver grass (Miscanthus sinensis) No. 1 was developed for production of bio-ethanol, and for the purpose the silver grass growing sector was established in Geumgang basin, Iksan, Jeonbuk, in 2011. However, the other application potentials except for using as the bio-energy resources should be considered because of the drop in international oil prices. Therefore, there is the necessity of a scientific basis to use the silver grass instead of rice straw as the organic matter source that is used for improvement of soil quality in the plastic film house. METHODS AND RESULTS: The silver grass was applied at 5, 10, 15 and 20 Mg/ha and tilled before the watermelon was planted in the plastic film-house. The control plot was treated with 10 Mg/ha with rice straw, and watermelons have been cultivated for 3 years(2017~2019). Soil aggregation, soil chemistry, and the growth characteristics were investigated, when the watermelon was harvested every year. Soil aggregation levels at the 2nd and 3rd year of watermelon harvest were similar from the plot applied with the silver grass at 5 Mg/ha and the control plot, and increased in the silver grass treated plots with more than 10 Mg/ha. However, there was no statistically significant difference between the plots. The nitrogen mineralization of silver grass in the control plot tended to be similar to the 5 Mg/ha plot, but the silver grass treated plots with over 10 Mg/ha showed low nitrogen mineralization. Soil EC on harvest stage was proportional to the applied mass of the silver grass, but pH was in inverse with the applied mass. Soil organic matter content, available phosphate, and exchangeable cations increased with the continued use of silver grass. Watermelon weight found to be the best on more than 15 Mg/ha of silver grass, and the sugar content was highest when 10 Mg/ha was treated. CONCLUSION: The use of the silver grass at 10 Mg/ha annually as the organic source was effective in replacing rice straw while growing fruits and vegetables on the plastic film house.

• 한국약용작물학회:학술대회논문집
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• 2012.05a
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• pp.115-116
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• 2012

• Proceedings of The Korean Society of Agricultural and Forest Meteorology Conference
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• 2018.06a
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• pp.124-125
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• 2018

• Asian-Australasian Journal of Animal Sciences
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• v.25 no.7
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• pp.903-912
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• 2012

In Erhualian and Yorkshire reciprocal cross $F_1$ pig populations, we examined the mRNA expression characteristic of liver-derived IGF-1, IGF-1R, IGF-2, IGF-2R and IGFBP-3 during the embryonic and postnatal developmental periods (E50, E70, E90, D1, D20, D70, D120 and D180). Our results demonstrated that the IGF-system genes mRNA levels exhibited an ontogenetic expression pattern, which was potentially associated with the porcine embryonic development, postnatal growth, organogenesis and even the initiation and acceleration of puberty. The expression pattern of IGF-system genes showed variation in the reciprocal cross ($F_1$ YE and EY pigs). This study also involved the expression features of imprinted genes IGF-2 and IGF-2R. The parent-of-origin effect of imprinted genes was reflected by their differential expression between the reciprocal crosses populations. The correlation analysis also indicated that the regulatory network and mechanisms involved in the IGF system were a complex issue that needs to be more fully explored. A better understanding of IGF system components and their interactive mechanisms will enable researchers to gain insights not only into animal organogenesis but also into somatic growth development and even reproduction.

• Korean Journal of Food Science and Technology
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• v.42 no.1
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• pp.45-49
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• 2010

To reduce the astringent taste of lotus root, the effects of various treatment methods such as drying, soaking, steaming and roasting on the physicochemical properties and sensory characteristics were investigated. The following process conditions were selected: 1) drying (D), 2) soaking followed by drying ($SK{\rightarrow}D$), 3) steaming followed by drying ($ST{\rightarrow}D$), 4) drying followed by roasting ($D{\rightarrow}R$), 5) soaking and then drying followed by roasting ($SK{\rightarrow}D{\rightarrow}R$), 6) steaming and then drying followed by roasting ($ST{\rightarrow}D{\rightarrow$}. The tannin content of the lotus root was lowest when it was treated by steaming followed by drying ($ST{\rightarrow}D$). The astringent taste of lotus root was reduced by steaming, and the roasted taste was improved by roasting in terms of sensory and flavor characteristics. Consequently, lotus root treated by steaming and then drying followed by roasting ($ST{\rightarrow}D{\rightarrow}R$) showed the highest preference with respect to astringent and roasted taste.

• The Korean Journal of Mycology
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• v.32 no.2
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• pp.105-111
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• 2004

The changes of microflora and chemical characteristics during fermentation process of cotton waste for oyster mushroom cultivation were investigated with 5 l bench-scale reactors placed in an incubator at different temperatures ($40,\;50\;and\;60^{\circ}C$). Cotton waste was wetted to 70% moisture, and air flow rates to the substrate were 50, 100 and 300 cc/min. In processing of composting, the mesophilic bacterial population decreased sharply but thermophilic bacterial population increased. In case of fungi, both mesophilic and thermophilic population decreased. The daily $CO_2$ evolution showed little difference in all treatments, while $NH_3$ dropped sharply after 3 days. The desirable composting temperature and air flow based on the mycelial growth of oyster mushroom were $50^{\circ}C$ and 100 cc/min, respectively.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.2
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• pp.123-129
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• 2006

A long-term experiment was carried out to examine the effects of compost on the long-term trends and synergy effects with chemical fertilizer and saving the chemical fertilizers of paddy cropping. The experiment was conducted for the past 36 years with constant amounts of N, P, K and compost in a paddy field at Milyang, South Korea. Grain yield was significantly increased in the plots with compost application. Twenty five years after the compost treatment, grain yield was significantly increased and it reached almost 90% of NPK plots. The effect of rice straw compost on grain yield was not clear during the early cropping years about 60%, but it slightly increased there after about 95% during the late five years. In compost plots, soil organic matter content, K and $SiO_2$ was greater in the compost applied plots than with application of recommended doses of NPK. However, soil pH was reduced in compost applied plots and Ca, and Mg were remained unchanged when compared to the application of recommended doses NPK. Soil nutrient contents were less in compost applied plots than with the application of recommended doses of NPK along with compost but was found to be increased than that of un-fertilized plots. The present study indicated that the application of rice straw compost with NPK is the best of all and followed by NPK and Compost. However, treatment of Compost is time consuming and hard working desired and eventually non economical practice in mechanized agricultural systems, even though Compost is very useful source of improving the soil fertility and its physical characteristics and also the application of inorganic N and P are essential for sustaining high yield.

• The Korean Journal of Mycology
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• v.17 no.3
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• pp.119-123
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• 1989

Interorder heterokaryons were obtained by polyethylene glycol induced fusion of protoplasts from auxotrophic mutants of Pleurotus ostreatus in agaricales and Ganoderma applanaturm in aphyllophorales. When transferred to MMM plates, all fusion colonies exhibited an extremely growth rate. During three times subcultivation on MCM the growth rate of fusants showed faster little by little. Seventy-five % fusion products of potoplasts showed mixed morphologies between those of P. ostreatus and G. applannatum in the first subcultivation on MCM and MGM. The phenotype of these fusants changed similar those of P. osteatus type after three times subcultivation on MCM. However, phenotype of 25% stable strains did not change on subcultivation. Hyphae of all fusion products did not form true clamp connection. All these types did not produce primordia. A comparrison of interorder somatic hybrids was made using isozyme analysis of esterase, malate dehydrogenase and peroxidase. In most cases the enzyme patterns of G. applanatum were not distinct, however, fusant showed non-parental bands.

• Journal of Animal Environmental Science
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• v.11 no.1
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• pp.11-16
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• 2005

The round bale wrappers are generally used for rice straw after the harvesting of low land rice by combine harvester. In this situation, the bale wrappers should be well adapted under the travelling over raised borders and temporary ditches in soft soil of narrow rice fields. The study was conducted to improve the performance of bale wrapper through the new design for compact size, lowered gravity center and lowered power consumption. The prototype of round bale wrapper had been designed and assembled to tractor with three point hitch mounted. The machine type is one roll driving system with one roll for rotating and one roll for wrapping. The driving torque and work performance of the machines were measured and analysed. The torque requirement of the prototype and conventional type was 6kgf-m and 12kgf-m, respectively. The prototype shaved less friction resistance between bale driving roll and round bale. and the power requirement can also be reduced from 12kgf-m in the conventional to 6kgf-m in the prototype. The work efficiency of the new bale wrapper was $45\%$ higher than the conventional wrapper, and the working cost of the prototype can be reduced $17\%$ than that of the conventional.