• Title/Summary/Keyword: Agricultural R&D

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Specific Detection of Vairimorpha spp. in Lepidoptera by Multiplex Polymerase Chain Reaction

  • Choi, Ji-Young;Je, Yeon-Ho;Kim, Jong-Gill;Choi, Young-Cheol;Kim, Won-Tae;Kim, Keun-Young
    • Proceedings of the Korean Society of Sericultural Science Conference
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    • 2003.10a
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    • pp.138-140
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    • 2003

  • We report the development of a multiplex PCR-based procedure for rapid, sensitive, and specific detection of entomopathgenic microspordia and differentiation of the genus Nosema and Vairimorpha and identification of the vairimorpha necatrix. (omitted)

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A Study on Survey for Technology Forecasting using Delphi in Biosystems Engineering (농업기계화분야의 델파이 기술예측조사에 관한 연구)

  • 이종인;조근태;장동일;이규천;조영우
    • Journal of Biosystems Engineering
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    • v.29 no.2
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    • pp.175-186
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    • 2004

  • The study was designed to forecast and derive future core technologies using Delphi method in Korea agriculture. The technologies will make agriculture for core and strategic industry that has high value-added in 21 century. Questions were given to specialists by each technology in order to survey importance, realization time, level of R＆D in Korea and foremost country, leading group of R＆D, effective policy, etc. for each technology. The target of the survey for Delphi is confined specialists in the area of Bioystems Engineering. 55 core technologies were derived and 31 specialists answered the survey.

  • https://doi.org/10.5307/JBE.2004.29.2.175 인용 PDF KSCI

Transformation of the ${\beta}-Isopropylmalate$ Dehydrogenase Gene of Flammulina velutipes into Pleurotus florida (팽나무버섯 Leu 2 gene(${\beta}-isopropylmalate$ dehydrogenase)의 사철 느타리버섯 형질전환)

  • Byun, Myung-Ok;Yoo, Young-Bok;Go, Seung-Joo;You, Chang-Hyun;Cha, Dong-Yul;Park, Yong-Hwan
    • The Korean Journal of Mycology
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    • v.17 no.1
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    • pp.27-30
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    • 1989

  • F. velutipes Leu 2 gene (${\beta}-isopropylmalate$ dehydrogenase gene) was used for transformation of P. florida leucine requiring auxotrophic mutant P101. Transformation frequency was very low but the transformed colony can grow on minimal medium very slowly. Transformation was identified by Southern hybridization and reverse transformation into E. coli using chromosome DNA isolated from transformed P. florida.

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Investigation of post-translational modification of the secreted protein expressed in insect cell lines using baculovirus expression vector system(BEVS)

  • Yun, Eun-Young;Goo, Tae-Won;Kim, Sung-Wan;Park, Kwang-Ho;Hwang, Jae-Sam;Kang, Seok-Woo;Kwon, O-Yu
    • Proceedings of the Korean Society of Sericultural Science Conference
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    • 2003.04a
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    • pp.82-83
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    • 2003

  • In previous experiment, we reported when the heterologous protein is expressed by using baculovirus expression vector system (BEVS), although the amount of intracellular protein is abundant, the amount of extracellular Protein is poor. As the link in the chain of the research, we investigated the secretory pathway, important in case of the secretory protein, of the protein expressed in insect cells using BEVS. (omitted)

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