• Journal of Microbiology and Biotechnology
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• v.25 no.12
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• pp.2082-2089
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• 2015

Avian beta-defensin 9 (AvBD9) is a small cationic peptide consisting of 41 amino acids that plays a crucial rule in innate immunity and acquired immunity in chickens. Owing to its wide antibacterial spectrum, lack of a residue, and failure to induce bacterial drug resistance, AvBD9 is expected to become a substitute for conventional antibiotics in the livestock and poultry industries. Using the preferred codon of Pichia pastoris, the mature AvBD9 peptide was designed and synthesized, based on the sequence from GenBank. The P. pastoris constitutive expression vector pGHKα was used to construct a pGHKα-AvBD9 recombinant plasmid. Restriction enzyme digestion was performed using SacI and BglII to remove the ampicillin resistance gene, and the plasmid was electrotransformed into P. pastoris GS115. High-expression strains with G418 resistance were screened, and the culture supernatant was analyzed by Tricine-SDS-PAGE and western blot assay to identify target bands of about 6 kDa. A concentrate of the supernatant containing AvBD9 was used for determination of antimicrobial activity. The supernatant concentrate was effective against Escherichia coli, Salmonella paratyphi, Salmonella pullorum, Pseudomonas aeruginosa, Enterococcus faecalis, and Enterobacter cloacae. The fermentation product of P. pastoris carrying the recombinant AvBD9 plasmid was adjusted to 1.0 × 10⁸ CFU/ml and added to the drinking water of white feather broilers at different concentrations. The daily average weight gain and immune organ indices in broilers older than 7 days were significantly improved by the AvBD9 treatment.

• Analytical Science and Technology
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• v.37 no.2
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• pp.79-86
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• 2024

Among the nutrients in feed, amino acids and minerals are important for the growth and development of pets. In particular, interest in nutritional components related to the health of pets is increasing as pet-raising households and pet food markets have recently grown. Therefore, in this study, 55 pet food products distributed in South Korea were purchased, and the content of 3 essential and conditionally essential amino acids (taurine, lysine, arginine) and 4 minerals (Ca, P, Na, K) was investigated. Among the three amino acids, arginine was found to have the highest content, and the average content was 1.79 and 1.37 % in cat and dog foods, respectively. On the other hand, the taurine content was the lowest, but it was found to be higher than the minimum requirement of 0.10 % for cats set by the American Association for Feed Control (AAFCO) and the European Federation of Pet Food Industries (FEDIAF). As a result of the four-component analysis of minerals, the content of Ca was found to be the highest, and the average content was confirmed to be 1.64 and 1.25 % in cat and dog food, respectively. On the other hand, Na was the lowest, but it was higher than the AAFCO minimum requirement and FEDIAF minimum requirement for young cat and dog food. Among all 55 samples examined, the content of the three amino acids and the four inorganic components was confirmed to be suitable for the recommended minimum intake and maximum allowable intake presented by AAFCO and FEDIAF.

• Journal of the Ergonomics Society of Korea
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• v.35 no.4
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• pp.205-223
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• 2016

Objective:This study introduced the status of studies and the projects related with agricultural accident prevention carried out by the Rural Development Administration. This study was conducted to promote Korean farmers' agricultural safety activation by analyzing study trends on farmers' agricultural work safety. Background: Agriculture is one of the dangerous industries, and the agricultural accident rate is on the rise, due to recent serious aging and increased female work force. Such a phenomenon emerges as a serious problem from the social perspective, and therefore measures for farmers' healthy and safe agricultural activities and accident prevention are required. Method: This study conducted literature review related with domestic and international agricultural safety and health. This study also examined the hazard factors of agricultural labor and injuries, agricultural safety and health system and policy, support and education for farmers' safety practice, and the status of convenience tools and protection equipment, based on major study activities performed by the Rural Development Administration for recent four to five years. Results: Through the study results, the status of Korea's agricultural safety system, compensation support policy, safety education of farmers for actual practice, and diverse channels including media were confirmed. This study also presented the R&D implementation status on convenience equipment and protective gear to prevent agricultural diseases and safety accidents. Conclusion: This study found out that continuous monitoring and efforts are required to prevent accidents for the Korea's agricultural labor environment and agricultural safety, and that social bond of sympathy formation is demanded through effect analysis on the implemented projects including education and support projects. Furthermore, it can be understood that agricultural accident prevention should be carried out through shift from current government-led top down approach to bottom-up approach from itself. Application: The results of this study can be utilized as useful data for farmers' safety and health.

• Journal of Agricultural Extension & Community Development
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• v.21 no.4
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• pp.1093-1124
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• 2014

Studies on the role of agricultural science colleges are mostly divided into agricultural production, which is the primary function of agriculture, and other functions, which have recently begun to be emphasized as a result of social needs. With the green revolution and the aging of the farming population, there is a strong view that the role of agricultural science colleges should remain as it is. However, agriculture is expanding in terms of concept and content by converging with other industries not traditionally associated with agricultural production. Thus, the fields that now need to form part of agricultural science knowledge are becoming more detailed and expansive. The government's perception remains at the level of merely fostering farmers. This was evident in a survey on the employment rate, a factor used to evaluate colleges, in which the role of agricultural science colleges was limited to fostering farmers. Agro- industry fields, other than agriculturalists, include general industries in which the academic fields of agricultural science are combined with other academic fields. Thus, even when someone is employed in an industry that requires background knowledge of agricultural science, there is often a perception that he or she is employed in a field that is irrelevant to the major. This study examines the role of agricultural science colleges in agriculture and farm villages by focusing on the employment of graduates of these colleges within agro-industry. We categorize academic research on agricultural science into 16 fields, based on the medium level of the National Standard Science and Technology Classification Codes. Then, we categorize the employment fields into 168 fields, based on the small classification level of the inter-industry relations classification. Thus, we investigate 220 departments of 37 colleges, nationwide. Our findings show that the average employment rate of graduates of agricultural science colleges is 69.0%. Furthermore, 33.0% of all employees work in agro-industry fields that require background knowledge in agricultural science, which is one out of three job seekers. Then, 3.6% of employees work in business startups in agro-industry. The aforementioned government survey showed that only 0.1% of all college graduates in Korea were employed as agriculturalists in 2013. However, our results showed that 13.3% of graduates were working as agriculturalists, which is significantly different to the results of the government survey. These results confirm that agricultural science colleges contribute greatly to the employment of graduates, including farmers, agro-industry, and business startups in agro-industry fields.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2000.11a
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• pp.76-100
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• 2000

A brief general discussion of the nature and function of biosensors is presented. While the primary motivator for biosensor development has been the health-care industries, recent research efforts have spread to problems in agriculture and biological production systems. To illustrate some of the research from our laboratory, three example biosensors and their corresponding applications are presented. The first of these is an immunosensor for measurement of the hormone progesterone during milking as a method to improve reproductive management of dairy herds. The second example is an enzyme sensor for measurement of urea in milk as a menas to determine the efficiency of conversion of input protein to milk protein and, thus, improve nutritional management of dairy herds. The third example is a DNA sensor using polymerase chain reaction to detect pathogenic bacteria in the wash water of fresh and minimally processed fruits and vegetables. The potential for application of biosensors in agriculture, agrobiotechnology, food processing, and environmental monitoring has barely been realized.

• Journal of Biosystems Engineering
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• v.39 no.1
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• pp.21-33
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• 2014

Background: Significant technological development and changes happened in the tractor industries. Contrariwise, the test procedures of the major standard development organizations (SDO's) remained unchanged or with a little modification over the years, demanding new tractor test standards or improvement of existing ones for tractor performance, safety, and comfort. Purpose: This study focuses on reviewing the research trends regarding performance, safety and comfort evaluation of agricultural tractors. Based on this review, few recommendations were proposed to revise or improve the current test standards. Review: Tractor power take-off power test using the DC electric dynamometer reduced human error in the testing process and increased the accuracy of the test results. GPS signals were used to determine acceleration and converted into torque. High capacity double extended octagonal ring dynamometer has been designed to measure drawbar forces. Numerical optimization methodology has been used to design three-point hitch. Numerous technologies, driving strategies, and transmission characteristics are being considered for reducing emissions of gaseous and particulate pollutants. Engine emission control technology standards need to be revised to meet the exhaust regulations for agricultural tractors. Finite Element Analysis (FEA) program has been used to design Roll-Over Protective Structures (ROPS). Program and methodology has been presented for testing tractor brake systems. Whole-body vibration emission levels have been found to be very dependent upon the nature of field operation performed, and the test track techniques required development/adaptation to improve their suitability during standardized assessment. Emphasizes should be given to improve visibility and thermal environment inside the cab for tractor operator. Tractors need to be evaluated under electromagnetic compatibility test conditions due to large growing of electronic devices. Research trends reviewed in this paper can be considered for possible revision or improvement of tractor performance, safety, and comfort test standards.

• Food Science and Biotechnology
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• v.14 no.1
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• pp.58-63
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• 2005

Ethyl acetate-soluble neutral fraction of hot water extracts from the aerial parts of Angelica keiskei showed a 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity. Six antioxidative compounds were purified and isolated by various chromatographic procedures. Based on the analyses of FAB-MS and NMR, the isolated compounds were structurally elucidated as luteolin 7-O-${\beta}$-D-glucopyranoside (1), quercetin 3-O-${\beta}$-D-galactopyranoside (2), quercetin 3-O-${\beta}$-D-glucopyranoside (3), quercetin 3-O-${\alpha}$-D-arabinopyranoside (4), kaempferol 3-O-${\alpha}$-D-arabinopyranoside (5), and luteolin 7-O-rutinoside (6). The glycosides of flavonols and luteolin showed DPPH radical-scavenging activity. One molecule of 2, 3, 4, 6, 1, and 5 scavenged 4.2, 4.2, 4.1, 2.5, 2.2, and 1.4 molecules of DPPH radical, respectively.

• Journal of Agricultural Extension & Community Development
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• v.11 no.2
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• pp.267-277
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• 2004

This study was carried out to set up the effective development strategy for resources industry of Chungnam Province. Data were gathered from relevant organizations and several times of brain storming was conducted by specialists in the field of animal science. The results obtained were as follows; 1) It was found by SWOT analysis that pig, cow, poultry, pets and selected fishes were desirable items as promising animal resources(Table 9). 2) According to the road map of animal resources industry for Chungnam Province, the principles and strategies were needed for the implementation of the industry(Fig. 3). 3) It was recommended that each city and county should develop their major animal resources industry(Fig. 4). 4) For synergy effect of development strategy as animal resources industry, networking among universities, industries, and government organizations, etc. is needed.

• Journal of Korean Society of Rural Planning
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• v.12 no.3 s.32
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• pp.67-80
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• 2006

The concept of RIS(Regional Innovation System) has widely been used in discourse and policy issues in Korea. But most studies of RIS concern on BT or IT industries combining with diverse regional agencies in urban areas. Some other studies were concentrated on general ideas or concept of the RIS. The purposes of this study are (1) to suggest a analytical method to select specialized industry in local autonomy, (2) to analyze the relationship between specialized industry and gross sales of agricultural products and stock farm products, (3) to analyze the relationship between gross sales of agricultural products and stock farm products and GRDP, and (4) to construct a model of RIS that fits particularly for rural areas. This study particularly accentuates that a specialized industry is more meaningful when it can raise GRDP, which eventually can give positive effect of RIS on regions.

• Journal of Korean Society of Rural Planning
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• v.14 no.4
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• pp.1-9
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• 2008

Many urban people are attracted to the recreational opportunities and attractions of rural areas, such as beautiful scenery, lakes, mountains, forests, and resorts. Furthermore, rural development planning increasingly emphasizes the integration of resource extractive industries with non-market-based recreational and amenity values. This article outlines a method to estimate an amenity level of rural areas such as natural amenities index and shows the relation of the natural amenity and population growth. The results revealed that natural amenity classifying each region can be explained with geographical characteristics, temperature-humidity, and sunshine duration time. In analysis with population, natural amenity does not exactly explain that but can be one of the important factor of population change.