• 농약과학회지
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• 제8권4호
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• pp.299-308
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• 2004

토양/벼 microecosystem내에서 sulfonylurea계 제초제 bensulfuron-methyl의 행적을 구명하기 위하여 [Phenyl-$^{14}C$]bensulfuron-methyl의 신생(fresh) 잔류물과 13주간 숙성된 숙성(aged) 잔류물을 처리한 2종의 상이한 논토양을 stainless steel pot (내경, 17 cm $\times$ 높이 10 cm)에 담고 벼 (Oryza sativa L.)를 12주간 재배 하였다. 토양 A(유기물 함량, 3.59%; 양이온 치환용량, 7.65 $cmol^+\;kg^{-1}$; 토성, 사질 식양토)와 토양 B(유기물 함량, 1.62%; 양이온 치환용량, 4.51 $cmol^+\;kg^{-1}$; 토성, 사양토)에서 $[^{14}C]$bensulfuron-methyl이 13주간의 숙성 기간동안 $^{14}CO_2$로 무기화된 량은 최초 처리량의 각각 6.79와 10.15% 이었다. 신생 잔류물을 함유한 토양으로부터 방출된 $^{14}CO_2$량은 숙성 잔류물을 처리한 토양으로부터 방출된 량보다 많았다. 12주간 벼를 재배하고 수확하였을 때 신생 잔류물을 함유한 토양 A와 B로부터 벼가 흡수 이행한 $^{14}C$량은 각각 최초 처리량의 1.53과 4.40%이었고 숙성 잔류물을 함유한 토양 A와 B로부터 흡수 이행한 양은 최초 처리량의 4.04와 6.37% 이었다. 숙성과 토성에 관계없이 토양에 잔류하고 있는 $^{14}C$량은 최초처리량의 $80.41\sim98.87%$ 이었다. 유기 용매로는 토양에 잔류하는 $^{14}C$ 방사능의 $39.25\sim70.39%$를 추출할 수 있었고 bensulfuron-methyl의 추출불가 토양 흡착 잔류물의 대부분은 fulvic acid 부분에 혼입되어 있었다 $(61.32\sim76.45%)$. 벼 재배 유무에 따른 미생물 활성을 비교한 결과 벼 재배시 두 토양에서 모두 미생물 활성이 벼를 재배하지 않은 경우의 $1.6\sim3.0$ 배 이었으나 $^{14}CO_2$ 발생량과는 반드시 일치하지 않았다.

• 동의생리병리학회지
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• 제17권2호
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• pp.529-541
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• 2003

Yukmijihwang-tang has been widely used as an and-aging herbal medicine for hundred years in Asian countries. Numerous studies show that Yukmijihwangtang has anti-oxidative effect both in vivo and in vitro. It has been reported that Moutan Cortex Radicis extract (MCR) was the most effective herb in Yukmijihwang-tang on undifferentiated PC12 cells upon oxidative-stressed with hydrogen peroxide. The purpose of this study is to; 1) evaluate the recovery of neuronal damage by assessing the anti-oxidant effect of MCR on PC12 cells differentiated with nerve growth factor (NGF), 2) identify candidate genes responsible for anti-oxidative effect on differentiated PC12 cells by oligonucleotide chip microarray. PC12 cells, which were differentiated by treating with NGF, were treated without or with hydrogen peroxide in the presence or absence of various concentration of MCR. Cell survival was determined by using MTS assay. Measurement of intracellular reactive oxygen species (ROS) generation was determined using the H2DCFDA assay The viability of cells treated with MCR was significantly recovered from stressed PC12 cell. In addition, wide rage of concentrations of MCR shows dose-dependent inhibitory effect on ROS production in oxidative-stressed cells. Total RNAs of cells without treatment(Control group), only treated with H₂O₂ (stressed group) and treated with both H₂O₂ and of MCR (MCR group) were isolated, and cDNAs was synthesized using oligoT7(dT) primer. The fragmented cRNAs, synthesized from cDNAs, were applied to Affymetrix GeneChip Rat Neurobiology U34 Array. mRNA of Calcium/calmodulin-dependent protein kinase II delta subunit(CaMKII), neuron glucose transporter (GLUT3) and myelin/oligodendrocyte glycoprotein(MOG) were downregulated in Stressed group comparing to Control group. P2X2-5 receptor (P2X2R-5), P2X2-4 receptor (P2X2R-4), c-fos, 25 kDa synaptosomal attachment protein(SNAP-25a) and GLUT3 were downregulated, whereas A2 adenosine receptor (A2AR), cathechol-O-methyltransferase(COMT), glucose transporter 1 (GLUT1), EST223333, heme oxygenase (HO), VGF, UI-R-CO-ja-a-07-0-Ul.s1 and macrophage migration inhibitory factor (MIF) were upregulated in MCA group comparing to Control group. Expression of Putative potassium channel subunit protein (ACK4), P2X2A-5, P2X2A-4, Interferon-gamma inducing factor isoform alpha precursor (IL-18α), EST199031, P2XR, P2X2 purinoceptor isoform e (P2X2R-e), Precursor interleukin 18 (IL-18) were downregulated, whereas MOO, EST223333, GLUT-1, MIF, Neuronatin alpha, UI-R-C0-ja-a-07-0-Ul.s1, A2. adenosine receptor, COMT, neuron-specific enolase (NSE), HO, VGF, A rat novel protein which is expressed with nerve injury (E12625) were upregulated in MCR group comparing to Stressed group. The results suggest that decreased viability and AOS production of PC12 cell by H₂O₂ may be, at lease, mediated by impaired glucose transporter expression. It is implicated that the MCR treatment protect PC12 cell from oxidative stress via following mechanisms; improving glucose transport into the cell, enhancing expression of anti-oxidative genes and protecting from dopamine cytotoxicity by increment of COMT and MIF expression. The list of differentially expressed genes may implicate further insight on the action and mechanism behind the anti-oxidative effects of herbal extract Moutan Cortex Radicis.

• 한국방사선학회논문지
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• 제14권1호
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• pp.15-22
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• 2020

PET(Positron Emission Tomography)장치는 해부학적 정보를 얻기 위한 CT와 MRI의 장치를 PET 장치와 융합시킨 PET/CT나 PET/MRI 장치로 사용된다. 그렇기 때문에 일반 의료장비들과 비교하여 Gantry(촬영부 본체)의 길이가 늘어나고 내부의 공간이 넓어지는 원형(ring)타입의 구조로 제작된다. PET 장치의 구성요소 중 하나인 신틸레이터는 Gantry 안쪽에 위치하는데 온·습도 변화에 민감한 크리스탈 성분으로 되어있기 때문에 커다란 온도 변화로 인해 신틸레이터의 손상을 가져올 수 있다. 신틸레이터는 항온항습기로 PET장치가 설치되어 있는 공간에 온·습도를 유지하지만 이러한 PET 장치의 구조적 원인과 CT 장비에서 발생하는 열로 인한 Gantry 내부의 온·습도의 변화가 예상된다. 항온항습기를 가동하여 온·습도를 유지중인 PET/CT 장치의 Gantry 내부를 6개의 구역으로 나눈 후 각 구역에 Arduino 온·습도 센서인 Adafruit BME 280을 부착하여 출력 값을 분석했다. 각 6개 구역에서 센서를 이용해 측정한 평균 온·습도 값과 항온항습기의 평균 측정값을 비교하면 평균적으로 온도는 2.71℃ 상승, 습도는 21.5% 감소의 차이가 발생하였고, PET Gantry의 온·습도 측정값은 국내에서 지정한 정도 관리 범위에서 벗어나는 결과 값이 나왔다. 연구 결과를 통해 앞으로 지속적인 온·습도의 변화를 가진다면 PET Gantry 안에 장착된 신틸레이터의 노후화가 예상되므로 Gantry구조 내부의 온·습도를 적절히 유지하는 방안을 모색해야 한다.

• 생명과학회지
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• 제25권1호
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• pp.62-67
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• 2015

본 연구는 바위수염 메탄올 추출물이 3T3-L1 지방세포의 분화 및 지방생성의 억제에 미치는 영향을 탐색하고자 하였다. 바위수염 메탄올 추출물의 농도에 따른 3T3-L1 세포의 성장에 미치는 영향을 MTT assay로 분석한 결과 $100{\sim}500{\mu}g/ml$l의 농도에서는 80% 이상의 비슷한 수준의 세포생존율을 보였다. 바위수염 메탄올 추출물을 처리하지 않고 분화를 유도하였을 경우에 세포질 내 지방구의 형성이 활발하게 유발되는 것으로 관찰되었으며, 바위수염 메탄올 추출물에 의한 지방구의 형성이 처리 농도 의존적으로 억제 되는 것을 확인하였다. 또한 바위수염 메탄올 추출물을 100, 300, $500{\mu}g/ml$ 처리한 후의 중성지방의 양은 바위수염 메탄올 추출물을 처리하지 않았을 때보다 35%, 42%, 76%로 농도 의존적으로 억제되는 것을 확인하였다. Insulin, dexamethasone 및 IBMX를 처리하여 분화를 유발하였을 경우 $PPAR{\gamma}$, $C/EBP{\alpha}$ 및 $C/EBP{\beta}$의 발현이 현저하게 증가되었으며, 이러한 분화유도 과정에서 바위수염 추출물을 처리한 결과 $100{\mu}g/ml$의 농도보다 $500{\mu}g/ml$의 처리군에서 $PPAR{\gamma}$, $C/EBP{\alpha}$ 및 $C/EBP{\beta}$의 발현이 단백질 수준에서 감소하였다. 본 연구결과 바위수염 메탄올 추출물은 lipid droplet 및 TG 생성을 감소시킴으로써 지방세포로의 분화를 억제시키는 것으로 나타나, 항비만 식품 소재로서의 개발 가능성이 있는 것으로 사료된다.

• 생명과학회지
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• 제19권1호
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• pp.58-64
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• 2009

본 연구는 사육환경 조건에서 참수리(Haliaeetus pelagicus)의 연령에 따른 날개깃의 변화 과정과 깃갈이 순서를 파악하기 위하여 수행되었으며, 이를 위해 2000년 11월부터 2005년 12월까지 약 5년간 경성대학교 조류연구소에서 참수리 유조를 사육하면서 조사한 자료를 토대로 하여 분석하였다. 최초의 날개깃 깃갈이는 생후 둘째 해 7월에 시작하였으며, 첫째날개깃 P1-3과 둘째날개깃 S18-19 (암컷), S17-18 (수컷), S1, S4 등 몇 개 정도만 교체되었다. 겨울 동안에는 깃갈이의 진행이 멈추었지만, 몇 개의 깃은 겨울에도 교체되었다. 둘째날개깃의 가장 안쪽 S18-19 (암컷), 17-18 (수컷) 등 2개의 깃은 해마다 깃갈이가 진행되었고 몇 개의 유조깃(수컷: S10, S11 등)은 2-3년 동안 교체되지 않았다. 깃갈이의 순서는 첫째날개깃의 경우 P1에서 시작하여 바깥쪽 P10의 방향으로 진행되었다. 둘째날개깃은 최초 S18(수컷), S19 (암컷)에서 시작해 바깥쪽으로 진행되었고, 이어서 S1에서 시작하여 안쪽으로 진행되는 방식이었다. 그 외에 둘째날개깃 중앙 부위에서 진행되는 깃갈이 방식은 보통 여러 곳에서 동시에 시작되었고, 이는 개체 또는 환경에 따라 차이가 있기 때문에 판단하기가 어려웠다. 날개깃의 생장기간은 가장 긴(P7, P8) 깃은 68일 이상이었다. 날개깃의 세대별 길이 차이는 유조깃과 2세대 깃 사이에 감소의 폭이 가장 컸고, 2세대와 3세대 깃 사이의 감소의 폭은 작았으며 3세대와 4세대 깃의 사이의 감소는 거의 나타나지 않았다. 유조의 첫째날개깃은 균일한 흑갈색이고 깃의 기부에는 흰색 반정이 있었으나 이것은 2세대 깃(수컷: 3차 겨울깃) 또는 3세대 깃(암컷: 4차 겨울깃)까지 나타나고 그 후에는 더 이상 나타나지 않았다.

• 대한수의학회지
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• 제23권2호
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• pp.137-148
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• 1983

This study was made for the better information of the male reproductive system on the meat-type drake, Cherry Belly X White Golden. The epithelium of ductules of epididymal region and deferent duct were observed histologically and histochemically with the progress of their development. India-ink absorbability on the luminal epithelium was also investigated after the administration of India-ink. The results are as follows; 1. Rete testis and various round ductules in immature form appeared in epididymis within 6 weeks after hatching, and simple cuboidal and simple columnar epithelium were found in the epithelia of the ductules within 8 weeks after hatching. Larger ductules were found on epididymal surface which was in the developing stage near to the immature efferent ductule. From 10th to 20th week, various ductules appeared in epididymis, and developing form of efferent ductules were much more increased on epididymal surface. The luminal epithelium of the ductules were composed of ciliated simple columnar and pseudostratified ciliated columnar cells. At the same time, deferent duct appeared. From the 21th week, various ductules in epididymis became abruptly matured. Lumen of rete testis was lined by simple squamous or simple cuboidal epithelium, and that of efferent ductules, having many folds and being larger than any others were lined by pseudostratified ciliated columnar epithelium in which ciliated columnar cells, non-ciliated cells(clear cells) and basal cells were noted. Connecting tubules of star shaped lumen were composed of pseudostratified ciliated columnar epithelium in which ciliated columnar cells, nonciliated cells, and basal cells were observed. The luminal surface of epididymal ducts was smooth and has thick pseudostratified columnar epithelium which was composed of high columnar cells and basal cells. From 26th week after hatching, sperm pooling was started in various ductules. 2. From 4th to 10th week, simple cuboidal epithelium of deferent duct transformed to simple columnar epithelium with the progress of aging. At the basement of epithelium, clear round cells were noted. From 12th to 20th week, high columnar cells with enlongated nucleus were noted on the luminal border of deferent ducts, forming folds of pseuclostratified columnar epithelium. From 20th week, the deferent duct started to have septa in it's lumen and composed mainly of pseudostratified columnar epithelium, and round cells disappeared. From 20th week, the lumen diameter of deferent duct became wider with the progress of aging, but there was no difference among the values of lumen diameter in upper, middle, and lower part of deferent ducts. At 26th week, the pooling period of sperms in deferent ducts, the lumen diameter became rapidly widen, especially in the lower part of deferent ducts. Thickness of muscular layer of ductus deferens showed gradual growth within 24 weeks but did abrupt thickening from 26th week. 3. Saliva resistant PAS granules were dotted on the top of nucleus in efferent ductules epithelium but the amount of the granules were little in the connecting ductules's epithelium. The granules reactive to acid phosphatase were abundant in the some epithelial cells of efferent ductules and connecting ductules, especially above the nucleus of cells. The granules reactive to alkaline phosphatase were noted on the luminal border of efferent ductules. Parts of free border of efferent ductules and middle portion of deferent ducts were stained slightly by alcian blue technique. India ink granules were found mainly in the epithelium of efferent ductules but were few in that of connecting ductules.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2008년도 International Meeting of the Microbiological Society of Korea
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• pp.39-41
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• 2008

The yeast Saccharomyces cerevisiae has been shown to contain three isoforms of citrate synthase (CS). The mitochondrial CS, Cit1, catalyzes the first reaction of the TCA cycle, i.e., condensation of acetyl-CoA and oxaloacetate to form citrate [1]. The peroxisomal CS, Cit2, participates in the glyoxylate cycle [2]. The third CS is a minor mitochondrial isofunctional enzyme, Cit3, and related to glycerol metabolism. However, the level of its intracellular activity is low and insufficient for metabolic needs of cells [3]. It has been reported that ${\Delta}cit1$ strain is not able to grow with acetate as a sole carbon source on either rich or minimal medium and that it shows a lag in attaining parental growth rates on nonfermentable carbon sources [2, 4, 5]. Cells of ${\Delta}cit2$, on the other hand, have similar growth phenotype as wild-type on various carbon sources. Thus, the biochemical basis of carbon metabolism in the yeast cells with deletion of CIT1 or CIT2 gene has not been clearly addressed yet. In the present study, we focused our efforts on understanding the function of Cit2 in utilizing $C_2$ carbon sources and then found that ${\Delta}cit1$ cells can grow on minimal medium containing $C_2$ carbon sources, such as acetate. We also analyzed that the characteristics of mutant strains defective in each of the genes encoding the enzymes involved in TCA and glyoxylate cycles and membrane carriers for metabolite transport. Our results suggest that citrate produced by peroxisomal CS can be utilized via glyoxylate cycle, and moreover that the glyoxylate cycle by itself functions as a fully competent metabolic pathway for acetate utilization in S. cerevisiae. We also studied the relationship between Cit1 and apoptosis in S. cerevisiae [6]. In multicellular organisms, apoptosis is a highly regulated process of cell death that allows a cell to self-degrade in order for the body to eliminate potentially threatening or undesired cells, and thus is a crucial event for common defense mechanisms and in development [7]. The process of cellular suicide is also present in unicellular organisms such as yeast Saccharomyces cerevisiae [8]. When unicellular organisms are exposed to harsh conditions, apoptosis may serve as a defense mechanism for the preservation of cell populations through the sacrifice of some members of a population to promote the survival of others [9]. Apoptosis in S. cerevisiae shows some typical features of mammalian apoptosis such as flipping of phosphatidylserine, membrane blebbing, chromatin condensation and margination, and DNA cleavage [10]. Yeast cells with ${\Delta}cit1$ deletion showed a temperature-sensitive growth phenotype, and displayed a rapid loss in viability associated with typical apoptotic hallmarks, i.e., ROS accumulation, nuclear fragmentation, DNA breakage, and phosphatidylserine translocation, when exposed to heat stress. Upon long-term cultivation, ${\Delta}cit1$ cells showed increased potentials for both aging-induced apoptosis and adaptive regrowth. Activation of the metacaspase Yca1 was detected during heat- or aging-induced apoptosis in ${\Delta}cit1$ cells, and accordingly, deletion of YCA1 suppressed the apoptotic phenotype caused by ${\Delta}cit1$ mutation. Cells with ${\Delta}cit1$ deletion showed higher tendency toward glutathione (GSH) depletion and subsequent ROS accumulation than the wild-type, which was rescued by exogenous GSH, glutamate, or glutathione disulfide (GSSG). Beside Cit1, other enzymes of TCA cycle and glutamate dehydrogenases (GDHs) were found to be involved in stress-induced apoptosis. Deletion of the genes encoding the TCA cycle enzymes and one of the three GDHs, Gdh3, caused increased sensitivity to heat stress. These results lead us to conclude that GSH deficiency in ${\Delta}cit1$ cells is caused by an insufficient supply of glutamate necessary for biosynthesis of GSH rather than the depletion of reducing power required for reduction of GSSG to GSH.

• 생명과학회지
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• 제26권1호
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• pp.50-58
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• 2016

왕느릅나무(Ulmus macrocarpa)의 껍질을 말린 유근피는 오랫동안 부종, 감염 및 염증 제어의 목적으로 사용되어져 왔음에도 불구하고 잠재적 면역조절 효과에 관해서는 연구가 이루어진 바 없다. 본 연구에서는 전통 약용자원에서 새로운 면역기능 증가 신소재 발굴의 일환으로 유근피 열수 추출물의 면역 조절 효능을 RAW 264.7 대식세포 모델을 이용하여 조사하였다. 이를 위한 대식세포의 활성화 관련 지표로서 NO, TNF-α, IL-1β 및 IL-10의 생성량 변화를 조사하였다. 비록 유근피 추출물이 처리된 RAW 264.7 대식세포에서 IL-1β의 유의적인 유리는 관찰할 수 없었으나, NO, TNF-α 및 IL-10의 생성은 세포독성을 나타내지 않는 범위에서 유근피 추출물 처리 농도 의존적으로 증가되었으며, 이는 또한 iNOS, TNF-α 및 IL-10의 단백질 발현 증가와 연관되어 있었다. 아울러 유근피 추출물은 LPS에 의한 과도한 NO의 생성 억제능도 함유하고 있었으며, 유근피 추출물에 의한 대식세포의 활성화에는 NF-κB와 PI3K/Akt 및 MAPKs 등과 같은 면역 활성을 유도하는 신호전달계의 활성화가 연관되어 있음을 알 수 있었다. 따라서 본 연구의 결과는 유근피 추출물이 대식세포 활성화를 통한 면역 증강제로서의 개발 가능성이 매우 높음을 시사한다.

• 동국한의학연구소논문집
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• 제4권
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• pp.161-187
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• 1995

This study was perfomed to investigate causes of the senile hypotension, pathogenic mechanism, symptoms, and therapies through medical literatures, recent chinese medical literatures and chinese medical journals. The results are as follows ; 1. The senile hypotension has major symptoms of dizziness, weakness, syncope, palpitation, shortness of breath, and deficiency of Qi. Additionally, it has minor symtoms of letharhy, isomnia, tinnitus, amnesia etc... 2. The prodromal symptoms of Kwul and Kwul are relating to the symptoms of tachycardia, facial pallor, sweating, anxietas, ambiguous consciousness, and fainting. Weakness and dizziness due to deficiency make the symptoms of exhaustion, fatigue, vertigo, lethargy, and brachycardia. 3. The most principal cause of the senile hypotension is deficiency of Shen due to aging, congenital deficiency, and chronic illness. The rest of causes are defciency of Qi and blood, phlegm of retention, stagnation of Qi, blood stasis, blood prostration etc... In the view of the occidental meicine, the causes of the senile hypotension came from the reduction of cardiac output, the decretion of cardiovascular system's extention due to aging, hereditary factor, secondary factor due to exsanguination, diabetes mellitus, C.V.A etc..., and factor of neurogenic system's degeneration. 4. The principal pathogenic mechanisms are the insufficiency of Xing-Yang, the deficiency of Qi in middle jiao, and deficiency of Shen-Qi. The rest of mechanisms are the deficiency of both Qi and blood, stagnation of the Gan-Qi, and the deficiency of Gan and Shen. Zang-Pu Organs have something to do with Xing, Bi, and Shen. 5. As principal therapies, there are warming and recuperation the Xing-Yang, strengthing the middle-jiao and replenishing Qi, replenishing vital essence to tonify the Shen, and warming and recuperation the Shen-Yang. Additionally, the therapies of invigorating the Bi and relieving mental stress, strengthning the Bi and tonifing the Shen, invigorating Qi and nourishing Yin, soothing the Gan and regulating the circulation of Qi, and tonifing the Shen and nourishing the Gan help the cure of the senile hypotension. In prescriptions there are Baohe Yuan Tang, Buzhong Yigi Tang, Zuoguei Yin, Yougui Yin, Guipi Tang, Zhu Fu Tang, Shengmai San, Sini San, and Qi Ju Dihuang Wan. The medical herbs of Astragali Radix, Codonopsitis Pilosulae Radix, Ginseng Radix, Aconiti Tuber, Ephedrae Herba, Cinnamomi Ramulus, Cinnamomi Corfex Spissus, Zingiberis Rhizoma, Polygalae Radix, Liriopis Tuber, Polygonati Sibirici Rhifoma, Lycii Fructus, Schizandrae Fructus, and Glycyrrhizae Radix can be treated. 6. According to the clinical report, the principal causes are the deficiency of Qi, and insufficiency of Yang which symptoms are dizziness, vitality fatigue and acratia, amnesia, body cold and alger of extremity, spontaneous perspiration, and therady and weak pulse. It was improved by taking WenYang YiQi Tang, Zhu Fu Tang about 20-30 days. The improvement was shown on disappearance of subjective symptoms or the ascending of blood pressure to normal figure, and the rate of improvement was over 70%. 7. As regimens, taking warming and recuperating food(a sheep mutton, juglans regia, chiness date, longan aril etc...) and pungent food(chinese green onion, fress ginger, pipers fructns etc...), doing physical training, not being ill in bed at a long time, and preventing descent of blood pressure coming from sudden change of posture are needed. Additionally, the usage of diuretic, abirritant, and depressor needs to be extra cautious.

• 생명과학회지
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• 제25권3호
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• pp.293-298
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• 2015

근위축은 근육 단백질 합성의 저하와 근육 단백질의 분해 증가에 따른 근섬유의 감소에 의한 근육량이 감소되는 현상이다. 오미자(Schisandrae Fructus, fruits of Schisandra chinensis (Turcz.) Baillon)는 오랫동안 전통의학에서 강장제로서 널리 사용되어 왔다. 비록 다양한 질병 연관 오미자의 생리활성 효능이 폭넓게 연구되어져 왔으나 근육 질환 관련 연구는 매우 제한적으로 이루어져 왔다. 본 연구에서는 오미자 에탄올 추출물(SF)이 AMPK 활성인자 AICAR 처리에 의한 C2C12 근관세포의 근위축 모델계를 이용하여 근위축 억제 효능을 가지는지의 여부와 관련 기전의 해석을 시도하였다. AICAR 처리는 근단백질 분해 연관 ubiquitin ligase muscle RING finger-1 (MuRF-1)의 발현을 전사 수준에서 증가시켰고, MuRF-1 조절 전사인자의 하나인 forkhead box O3a (FoxO3a) 단백질의 인산화를 증가시켰으며, 이러한 변화는 근위축과 연관된 C2C12 근관세포의 형태적 변형과 동반된 현상이었다. 그러나 SF의 전처리에 의하여, AICAR에 의하여 유도된 근위축성 형태변화를 억제하였으며, MuRF-1의 발현과 FoxO3a의 활성화를 억제시켰다. 본 연구의 결과는 SF가 AICAR 처리에 의한 C2C12 근관세포의 근위축을 AMPK 및 FoxO3a 신호전달계 조절을 통하여 억제하였음을 보여주는 것으로 오미자는 근기능 향상을 위한 식의 약 소재로서의 개발 가능성이 매우 높음을 시사하여 준다.