• 제목/요약/키워드: Aggregation and Transmission

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An Epidemiologic Study on Related Factors to HBsAg and Anti-HBs (B형 바이러스 간염의 검사성적 및 관련요인에 관한 역학적 연구 -의료직을 포함한 직장인을 중심으로-)

  • Kim, Joo-Ja;Han, Gu-Woong;Nam, Taik-Sung
    • Journal of Preventive Medicine and Public Health
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    • v.19 no.1 s.19
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    • pp.91-99
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    • 1986
  • To determine relationships of supposed risk factors to positives for HBsAg and Anti-HBs and also relationships of subjective symptoms to positives for HBsAg and Anti-HBs, study of 658 people working in the hospital, university, bank and other office was performed. Positive rate for HBsAg was about 7.9% and positive rate for Anti-HBs was about 20.0%. Odds ratio of HBsAg was high and significant in individuals who are married and who have previous hepatitis B(P<0.001), medical personnel in family, more than 4 people in a room(0.01

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Antibiotic Resistance and Safety Assessment of Enterococcus faecium CKDB003 for Using as Probiotics (프로바이오틱스 Enterococcus faecium CKDB003의 항생제 내성 및 안전성 평가)

  • Kim, Han Jun;Kang, Soon Ah
    • The Korean Journal of Food And Nutrition
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    • v.33 no.3
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    • pp.223-236
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    • 2020
  • In this study, a safety evaluation was conducted to confirm if the Enterococcus faecium CKDB003 strain obtained by selection from a mixed fermentation of fruit and milk is suitable for use as a probiotic. The MIC value for the 10 antibiotics specified in the EFSA guidance was below the acceptable cut-off value. The antibiotic resistance genes aac(6')-li, eatAv, and msr(C) exist by whole genome sequencing, but are in the chromosome and not in the plasmid, thus confirming that there is no possibility of transmission to other microorganisms. It was confirmed that cytolysin (cylA, cylB, cylI, cylL-l, cylL-s, cylM, cylR1, cylR2), aggregation substance (asa1, asp1), collagen adhesion (ace), enterococcal surface protein (esp), endocarditis antigen (efaA), hyaluronidase (hyl) and gelatinase (gelE) were not present in the genome by examining the genes of factors related to virulence. Also, the biochemical analysis showed no toxic enzyme activities, and no virulence genes were detected by the PCR method. Thus, the E. faecium CKDB003 strain can be safely used as a health functional food probiotic, based on the results of the safety assessment.

Membrane-Associated Hexavalent Chromium Reductase of Bacillus megaterium TKW3 with Induced Expression

  • Cheung K.H.;Lai H.Y.;Gu Ji-Dong
    • Journal of Microbiology and Biotechnology
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    • v.16 no.6
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    • pp.855-862
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    • 2006
  • Hexavalent chromium ($Cr^{6+}$) is a highly harmful pollutant, which can be detoxified and precipitated through reduction to $Cr^{3+}$. Bacillus megaterium TKW3 previously isolated from chromium-contaminated marine sediments was capable of reducing $Cr^{6+}$ in concomitance with metalloids ($Se^{4+}$, $Se^{6+}$, and $As^{5+}$). Notwithstanding approximately 50% inhibition, it was the first report of simultaneous bacterial reduction of $Cr^{6+}$ and $Se^{4+}$ (to elemental Se). No significant difference was observed among electron donors (glucose, maltose, and mannitol) on $Cr^{6+}$ reduction by B. megaterium TKW3. The reduction was constitutive and determined to be non-plasmid mediated. Peptide mass fingerprints (PMF) revealed a novel aerobic membrane-associated reductase with $Cr^{6+}$-induced expression and specific reductive activity (in nmol $Cr^{6+}$/mg protein/min) of 0.220 as compared with 0.087 of the soluble protein fraction. Respiratory inhibitor $NaN_3$ did not interfere with the reductase activity. Transmission electron microscopy with energy dispersive X-ray (TEM-EDX) analysis confirmed the aggregation of reduced chromium along the intracellular membrane region. Future identification of the N-terminal amino acid sequence of this reductase will facilitate purification and understanding of its enzymatic action.

Markable Green Synthesis of Gold Nanoparticles Used As Efficacious Catalyst for the Reduction of 4-Nitrophenol

  • Rokade, Ashish A.;Yoo, Seong Il;Jin, Youngeup;Park, Seong Soo
    • Clean Technology
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    • v.26 no.4
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    • pp.251-256
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    • 2020
  • The biocompatibility and plasmonic properties of Au nanoparticles make them useful for photothermal therapy, drug delivery, imaging, and many other fields. This study demonstrated a novel, facile, economic, and green synthetic method to produce gold nanoparticles. Gold nanoparticles (AuNPs) with spherical and triangular shapes were effectively synthesized using only Schisandra chenesis fruit extract as the capping and reducing agent. The shape of the AuNPs could be engineered simply by adjusting the molar concentration of HAuCl4 in the reaction mixture. The as-synthesized AuNPs were characterized using UV-VIS spectroscopy, transmission electron microscopy (TEM), X-ray diffraction (XRD), dynamic light scattering (DLS), and energy dispersive X-ray analysis (EDXA). This study revealed that by using the HAuCl4 concentration in the AuNP synthesis, the shape and size of the AuNPs could be controlled by the concentration of HAuCl4 and Schisandra chinensis fruit extract as a surfactant. The as-synthesized AuNPs samples had sufficient colloidal stability without noticeable aggregation and showed the predominant growth of the (111) plane of face-centered cubic gold during the crystal growth. The catalytic efficiency of the AuNPs synthesized using Schisandra chenesis fruit extract was examined by monitoring the catalytic reduction of 4-nitrophenol to 4-aminophenol using Ultraviolet-visible spectroscopy (UV-Vis spectroscopy). The synthesized AuNPs showed good catalytic activity to reduce 4-nitrophenol to 4-aminophenol, revealing their practical usefulness.

Synthesis and Catalytic Applications of Ruthenium(0) Nanoparticles in Click Chemistry

  • Kumar, Avvaru Praveen;Baek, Min-Wook;Sridhar, Chirumarry;Kumar, Begari Prem;Lee, Yong-Ill
    • Bulletin of the Korean Chemical Society
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    • v.35 no.4
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    • pp.1144-1148
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    • 2014
  • Here we report a facile synthesis of ruthenium (Ru) Nanoparticles (NPs) by chemical co-precipitation method. The calcination of ruthenium hydroxide samples at $500^{\circ}C$ under hydrogen atmosphere lead to the formation of $Ru^0$ NPs. The size and aggregation of Ru NPs depends on the pH of the medium, and type of surfactant and its concentration. The X-ray diffraction (XRD), scanning electron microscope (SEM) and transmission electron microscope image (TEM) analyses of particles indicated the formation of $Ru^0$ NPs, and have 10 to 20 nm sizes. As-synthesized $Ru^0$ NPs are characterized and investigated their catalytic ability in click chemistry (azidealkyne cycloaddition reactions), showing good results in terms of reactivity. Interestingly, small structural differences in triazines influence the catalytic activity of $Ru^0$ nanocatalysts. Click chemistry has recently emerged to become one of the most powerful tools in drug discovery, chemical biology, proteomics, medical sciences and nanotechnology/nanomedicine. In addition, preliminary tests of recycling showed good results with neither loss of activity or significant precipitation.

Synthesis of Composite Particles with Fe3O4 core and Ag Shell for the Development of Fingerprints

  • Zhang, Ling-Yan;Chu, Ting
    • Bulletin of the Korean Chemical Society
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    • v.34 no.5
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    • pp.1457-1461
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    • 2013
  • The $Fe_3O_4$-core and Ag-shell ($Fe_3O_4@Ag$ nanoeggs) were prepared through the encapsulation of 3-aminopropyltriethoxysilane-coated magnetite nanoparticle in nano-Ag shell by a simple chemically controlled procedure. The $Fe_3O_4@Ag$ nanoeggs were characterized by scanning electron microscopy, transmission electron microscopy, UV-vis spectrum and superconducting quantum interference device magnetometer, respectively. A detailed analysis is provided of how the hydrolysis and condensation of 3-aminopropyltriethoxysilane and the pH value are vital in fabricating the $Fe_3O_4@Ag$ nanoeggs. The prepared $Fe_3O_4@Ag$ nanoeggs possessed uniform size, improved monodispersity, stability against aggregation and high magnetization, which were utilized for the detection of latent fingerprints deposited onto different surfaces. The experimental results showed that the latent fingerprints developed with the $Fe_3O_4@Ag$ nanoeggs powders exhibited excellent ridge details with minimal background staining.

Multi-homing in Heterogeneous Wireless Access Networks: A Stackelberg Game for Pricing

  • Lee, Joohyung
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.12 no.5
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    • pp.1973-1991
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    • 2018
  • Multimedia applications over wireless networks have been evolving to augmented reality or virtual reality services. However, a rich data size compared to conventional multimedia services causes bandwidth bottlenecks over wireless networks, which is one of the main reasons why those applications are not used widely. To overcome this limitation, bandwidth aggregation techniques, which exploit a multi-path transmission, have been considered to maximize link utilization. Currently, most of the conventional researches have been focusing on the user end problems to improve the quality of service (QoS) through optimal load distribution. In this paper, we address the joint pricing and load distribution problem for multi-homing in heterogeneous wireless access networks (ANs), considering the interests of both the users and the service providers. Specifically, we consider profit from resource allocation and cost of power consumption expenditure for operation as an utility of each service provider. Here, users decide how much to request the resource and how to split the resource over heterogeneous wireless ANs to minimize their cost while supporting the required QoS. Then, service providers compete with each other by setting the price to maximize their utilities over user reactions. We study the behaviors of users and service providers by analyzing their hierarchical decision-making process as a multileader-, multifollower Stackelberg game. We show that both the user and service provider strategies are closed form solutions. Finally, we discuss how the proposed scheme is well converged to equilibrium points.

Fabrication and Thermal Oxidation of ZnO Nanofibers Prepared via Electrospinning Technique

  • Baek, Jeong-Ha;Park, Ju-Yun;Kang, Ji-Soo;Kim, Don;Koh, Sung-Wi;Kang, Yong-Cheol
    • Bulletin of the Korean Chemical Society
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    • v.33 no.8
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    • pp.2694-2698
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    • 2012
  • Materials on the scale of nanoscale have widely been used as research topics because of their interesting characteristics and aspects they bring into the field. Out of the many metal oxides, zinc oxide (ZnO) was chosen to be fabricated as nanofibers using the electrospinning method for potential uses of solar cells and sensors. After ZnO nanofibers were obtained, calcination temperature effects on the ZnO nanofibers were studied and reported here. The results of scanning electron microscopy (SEM) revealed that the aggregation of the ZnO nanofibers progressed by calcination. X-ray diffraction (XRD) study showed the hcp ZnO structure was enhanced by calcination at 873 and 1173 K. Transmission electron microscopy (TEM) confirmed the crystallinity of the calcined ZnO nanofibers. X-ray photoelectron spectroscopy (XPS) verified the thermal oxidation of Zn species by calcination in the nanofibers. These techniques have helped us deduce the facts that the diameter of ZnO increases as the calcination temperature was raised; the process of calcination affects the crystallinity of ZnO nanofibers, and the thermal oxidation of Zn species was observed as the calcination temperature was raised.

Centralized mmWave-Based Multi-Spot Beam Cellular System (중앙 집중형 밀리미터파 기반 다중 스팟 빔 셀룰러 시스템)

  • Park, Soon-gi;Choi, Yong-seouk;Kim, Tae-joong
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.41 no.3
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    • pp.285-297
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    • 2016
  • In this paper, a centralized mmWave-based multi-spot beam cellular system architecture having such a base station equipped with a plurality of beam-forming antennas, multi-layered dynamic cell configuration method as one of key concepts of this system operation and a mobility management method based on it are introduced. To estimate the performance of new system, system simulation was performed under the ideal environment without blockage. These results show the possibility that system capacity can be dramatically increased and mobility performance similar to the existing cellular system may be achieved.

Morphological Analysis of the Dermal Wounds Healing Responses in Bombina orientalis (무당개구리 (Bombina orientalis) 진피 상처치유반응의 형태적 분석)

  • Jeong, Moon-Jin;Moon, Myung-Jin
    • Applied Microscopy
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    • v.28 no.3
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    • pp.379-390
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    • 1998
  • Dermal wound healing responses in the skin of the toad, Bombina orientalis, were examined using transmission electron microscopy. At 12 hours after wounding, debridement and collagenolysis occurred in damaged dermis. Histocyte has a large nucleus and long cytoplasm process. Phagocytic vesicle and lysosome were observed in the cytoplasm. Damaged blood cells were transformed spindle to irregular shape. Autolysis was observed in their cytoplasm. Histocytes are found in poly-band. The irregularly shaped nucleus is located peripheral region in cytoplasm. At 2 days after wounding, partial aggregation of blood cells is observed. Phagocytic, activity is observed in histocyte and collagenolytic collagen fibers are scattered. Fibroblast is observed in the dermis at 3 days after wounding. Clusters of ribosomes and some short cisternae of rough-surfaced endoplasmic reticulum are found in the cytoplasm. In histocyte at 7 days post wounding, various size granules composed of moderately dense material are found the cytoplasm. In this period histocyte is round to rod in profile, with slender processes projecting from the surface. At 7 day after injury, it was observed that formation of connective tissue fibers and amorphous ground substance in regenerating skin.

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