• Title/Summary/Keyword: Agent Identity

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Cloning and Nucleotide Sequence of the recA Gene from Shigella sonnei KNIH104S Isolated in Korea

  • Park, Yong-Chjun;Shin, Hee-Jung;Kim, Young-Chang
    • BMB Reports
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    • v.32 no.5
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    • pp.436-439
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    • 1999
  • Shigella sonnei is an important cause of human enteric infections. S. sonnei KNIH104S was previously reported to be isolated from Korean shigellosis patients. We cloned a 2.8-kb KpnI fragment containing the recA gene encoding a recombinase from the chromosomal DNA of S. sonnei KNIH104S. This recombinant plasmid was named pRAK28. E. coli HB101, a recA mutant, cannot grow on Luria-Bertani medium in the presence of the alkylating agent methylmethane sulfonate, however, E. coli HB101 harboring pRAK28 was found to grow on this medium. As far as we know, we are the first to sequence the recA gene from S. sonnei. This gene is composed of 1062 base pairs with an ATG initiation codon and a TAA termination codon. Nucleotide sequence comparison of the S. sonnei recA gene exhibited 99.7% and 99.5% identity with those of S. flexneri and E. coli, respectively.

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A Study on the Globaization Starategy of Korean Textile Design

  • Lee, Eun-Oak
    • The International Journal of Costume Culture
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    • v.4 no.1
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    • pp.61-70
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    • 2001
  • This study discuses the strategies for the reestabisgment of Korean textile industry by emphasizing the area of textile design which plays an important role in developing textile materials. The textile industry in Korea has been in stagnancy for a long period of time and appears to find no answer to the problem. The big part of the problem is that the industry itself does not realize the importance of textile design and its management does not understand its critical role in contributing the growth of the industry. To develop textile design, first, we need to foster textile designers who have the creativity, an understanding of international trend and a mind f management. This can be achieved through an education system with a close tie to the textile industry. Second, we need to globalize our textile design in three aspects : design, technology and marketing. A balanced and harmonized progression of these three aspects will guarantee a successful globalization of textile design. This progression, however, demands creativity, identity, and interaction on the design side, digital and networking on the technology side, and culture marketing, brand value, and global agent networking on the marketing side.

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Differentiation of Lymphocystis Disease Virus Genotype by Multiplex PCR

  • Kitamura Shin Ichi;Jung Sung-Ju;Oh Myung-Joo
    • Journal of Microbiology
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    • v.44 no.2
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    • pp.248-253
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    • 2006
  • Lymphocystis disease virus (LCDV) is the causative agent of lymphocystis disease. The viruses have been divided into three genotypes (genotype I for LCDV-1, II for Japanese flounder isolates, and III for rockfish isolates) on the basis of major capsid protein (MCP) gene sequences. In this study, we developed a multiplex PCR primer set in order to distinguish these genotypes. We also analyzed the MCP gene of a new LCDV isolate from the sea bass (SB98Yosu). Comparison of sequence identities between SB98Yosu and eight Japanese flounder isolates, revealed identity of more than 90.1 % at nucleotide level and 96.5% at deduced amino acid level, respectively. Phylogenetic analyses based on the MCP gene showed that SB98Yosu belongs to genotype II, along with Japanese flounder isolates. Multiplex PCR based on the MCP gene allowed us to identify these genotypes in a simple and rapid manner, even in a sample that contained two genotypes, in this case genotypes II and III.

A Design of Security Protocol for Mobile Agent Systems Applying the Identity-based Digital Signature Scheme (ID 기반의 디지털 서명 기술을 적용한 이동 에이전트 시스템의 보안 프로토콜 설계)

  • 김성열;한승조;배용근;정일용
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.26 no.10A
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    • pp.1706-1716
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    • 2001
  • 이동에이전트 시스템은 기하급수적으로 증가하는 분산처리 환경과 이동컴퓨팅에 기여할 수 있다는 점으로 인해 주목받고 있는 기술이지만, 심각한 보안문제를 안고 있다. 본 논문에서는 ID 기반의 디지털 다중 서명 기술을 이용하여 이동 에이전트 시스템의 보안문제에 대한 효율적인 해결책을 제시하고자 한다. 이를 위하여 본 연구는 이동 에이전트 시스템이 가질 수 있는 보안 위협 요소를 분석하였다. 이러한 보안 위협은 적절한 보안 기술의 적용으로 해결될 수 있을 것으로 판단된다. 따라서 본 논문은 여러 사용자가 공동의 메시지를 전자적으로 서명하는 보안 기술인 디지털 다중 서명(Digital Multi-signature)기법과 ID에 기반한 키(key) 분배 기법을 이용하여 이동 에이전트 시스템의 보안 문제를 해결하기 위한 새로운 방법을 제안한다. 제안된 이동 에이전트 보안 프로토콜은 키 분배 및 관리의 단순화, 인증 절차의 단순화, 에이전트 실행 플랫폼의 인증과 송수신 부인 방지 기능을 제공, 생명성을 보장, 실행 결과 데이터의 기밀성, 무결성의 보장한다. 그리고 에이전트 실해의 전 단계를 매 시스템마다 검증함으로써 변경 또는 삭제시 곧바로 탐지한다.

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Bremia itoana (Oomycota, Peronosporales), a Specialized Downy Mildew Pathogen on an East Asian Plant, Crepidiastrum sonchifolium (Asteraceae)

  • Choi, Young-Joon;Park, Ji Hoon;Lee, Jeongran;Shin, Hyeon-Dong
    • Mycobiology
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    • v.46 no.4
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    • pp.416-420
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    • 2018
  • Crepidiastrum sonchifolium, a flowering plant in the daisy family (Asteraceae), is native to East Asia. In Korea, this plant is a locally cultivated vegetable, and its market size is gradually growing. Since the plants with downy mildew infection were initially found at a private farm of Chuncheon city, the occurrences have continued in commercial farms of other regions, highlighting that this disease is spreading throughout Korea. The pathogen was attributed to a member of the genus Bremia that contains many specialized species, each of which displays a narrow host spectrum on Asteraceae. Based on morphological and molecular phylogenetic analyses, along with the high host specificity recently proven for Bremia species, the identity of the causal agent was confirmed as a so far undescribed species of Bremia. Here, we introduce Bremia itoana sp. nov., specific to C. sonchifolium.

Erysiphe quercicola Causing Powdery Mildew on Quercus rubra in Korea

  • Young-Joon Choi;Jun Hyuk Park;In-Young Choi;Lamiya Abasova;Joon-Ho Choi;HyeonDong Shin
    • The Korean Journal of Mycology
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    • v.50 no.2
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    • pp.137-141
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    • 2022
  • In Korea, powdery mildew has been observed on Quercus rubra, commonly called the northern red oak tree. Based on its morphological and molecular phylogenetic analyses, the fungal agent was identified as Erysiphe quercicola, but it was distinct from the other four Erysiphe species, specialized to Quercus species. This is the first confirmed report on the identity of powdery mildew found on Q. rubra in Korea. This finding can help understand the host expansion of E. quercicola and the biodiversity of powdery mildew on Quercus species.

First Report of Black Rot Caused by Diplodia seriata on Apple (Diplodia seriata에 의한 사과 검은썩음병 발생보고)

  • Kim, Young Soo;Yun, Yun Joo;Jeon, Yongho
    • Research in Plant Disease
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    • v.24 no.4
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    • pp.321-327
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    • 2018
  • In 2018, symptoms of black rot on apple (Malus pumila var. dulcissima KOIDZ) cv. Fuji were observed in Yeongcheon-si, Korea. The fruit decay symptoms consisted of purple pimples spots, black rot around the seed cavity (calyx end), mummified fruit. To isolate the causal agent, small fragment (2 to 3 mm) of decayed tissue from the lesion margin were placed on WA or PDA. Fungal colonies on PDA produced dense white aerial mycelium, becoming dark gray with age. Pycnidia and conidia were observed under a light microscopy. The shapes of conidia were aseptate, ovoid, rounded at both ends, and $21.7-28.3{\times}9.9-15.3{\mu}m$. Based on morphological and cultural characteristics, this fungus was identified as Diplodia seriata. To confirm its identity, two loci (ITS and ${\beta}$-tubulin) were sequenced for molecular identification. BLAST searches indicated 100% identity with D. seriata. A pathogenicity test was conducted with isolates on Fuji apples. The apples were inoculated with mycelial plugs (5 mm) from 7-day-old cultures of the putative pathogens. All inoculated apples developed rot symptoms identical to the original symptoms, from which D. seriata were reisolated, fulfilling Koch's postulates. This study is the first report of black rot caused Diplodia seriata on apple.

Genetic Identity of a Korean Isolate of an Endoparasitoid Cotesia plutellae(Hymenoptera: Braconidae), Among Reproductive Incompatibility Types (생식형불일치 유형에 따른 국내 프루텔고치벌(Cotesia plutellae)의 유전적 위치)

  • Park, Jung-A;Kim, Yong-Gyun
    • Korean journal of applied entomology
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    • v.46 no.1 s.145
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    • pp.57-62
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    • 2007
  • Reproductive incompatibility is an Important factor to select a specific biologlcal control agent for successful augmentation of the corresponding endogenous population. An endoparasitoid, Cotesia plutellae (Kurdjumov), is an effective control agent to diamondback moth, Plutella xylostella (L.) and has been known to be classified into two groups in terms of reproductive incompatibility. This study analyzed an Korean population of C. plutellae in terms of morphological characters and mitochondrial DNA marker, which did not match with either of two reproductive incompatibility groups. These results suggest that a Korean population of C. plutellae can be involved in a novel reproductive group. For any augmentation program of C. plutellae in Korea, reproductive incompatibility should be seriously considered to select a particular exotic population.

A Study on PIN-based Authentication and ID Registration by Transfer in AAA System (AAA시스템에서의 이동에 따른 PIN 기반의 인증 및 ID 등록에 관한 연구)

  • Kang Seo-Il;Lee Im-Yeong
    • The KIPS Transactions:PartC
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    • v.13C no.3 s.106
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    • pp.359-368
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    • 2006
  • AAA(Authentication, Authorization, Accounting) is the service that offers authentication, authorization, and accounting method, and every terminal that accesses the network requires this AAA service. The authentication process of a mobile terminal is as follows: a mobile phone accesses an authentication server in a home network via the authentication service in an external network, which receives the authentication result. And, for the home authentication server to offer secure service, a unique key is distributed for the secure communication between the external agent and the user, the external agent and the home authentication server, and the user and the home authentication server. This paper discusses and proposes the key distribution for secure communication among external authentication servers when a mobile terminal travels to an external network. As the proposed method does not require the home authentication server to reissue another authentication when a user travels to other external networks, it reduces the overload in the home authentication server. It can also distribute a PIN-driven key.

A Study on the Change of Identity and Agency of International Marriage Migrant Women Changing with the Social Positionality : A Case Study of Gumi (국제결혼이주여성의 정체성 및 주체성의 사회적 위치성에 따른 변화 -구미 지역의 국제결혼이주여성의 생애사 분석을 중심으로-)

  • Park, Shin-Kyu
    • Journal of the Korean association of regional geographers
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    • v.14 no.1
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    • pp.40-53
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    • 2008
  • A case study has been conducted on the changes in the identity and agency of international marriage migrant women who experienced a shift from a immigrant worker to a foreign spouse and a Korean citizen. The life histories of the eight female migrants living in Gumi area, a representative inland industrial complex in Korea, have been investigated by in-depth interview. The aim is to examine how the female migrants reconstruct and interpret their lives. The results reveal that the identy of a female migrant is flexible depending on her social positionality. The identities established from the past experiences in their native countries have been changed by their situations and conditions in Korea. The female immigrants recognize that their problems have been an important issue both in government policy and mass media. However, the female migrants express a strong revolt against the fact that they are considered as underclass victims or innocent people from underdeveloped countries. This implies their ambivalence toward international marriage that they selected subjectively. There is a finding to show a new possibility; the Transnational Marriage & Family Support Center supported by Government may provide a good ground for the female migrants to be a active group agent. The results illustrate that the international marriage migrant women could not be classified into a single group as wives. Called for are diverse researches reflecting the complex situations of migrant women.

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