• KOREAN POULTRY JOURNAL
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• v.17 no.6 s.188
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• pp.99-102
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• 1985

• Korean Journal of Veterinary Service
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• v.33 no.2
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• pp.185-197
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• 2010

This study was conducted to evaluate the alleviative effects of activated charcoal (AC) and Houttuynia cordata (HC) singly or in combination in broiler chickens during aflatoxicosis. Activated charcoal (1% or 0.5%) and H. cordata (1% or 0.5%) were mixed into the diets for the ability to reduced the deleterious effects of 2.4mg total aflatoxin $(AFB_1)kg^{-1}$ diet on growing broiler chickens from 1 to 21 days of age. A total of 160 1-day-old (Hyline Variety Brown) broiler chicks were housed in eight treatment groups [Control, $AFB_1$, AC 1%, HC 1%, $AFB_1$ plus AC 1% plus HC 1%, $AFB_1$ plus AC 1% plus HC 0.5%, $AFB_1$ plus AC 0.5% plus HC 1%, $AFB_1$ plus AC 0.5% plus HC 0.5%] each consisting of 20 chicks. Compared to control, 2.4mg $AFB_1$ alone treatment group significantly decreased body weight gains of chickens. The addition of mixed AC 1% and HC 1% including 6, 7 groups to the 2.4mg $AFB_1$-containing diet moderately reduced the adverse effects of $AFB_1$ on performances of chickens. The chickens consuming 2.4mg $AFB_1$ plus AC 0.5% and HC 0.5%-containing diet showed very slightly reduced the adverse effects on investigated parameters compared to the $AFB_1$ only treated group. Also, the single addition of AC or HC to the $AFB_1$-free diet had no adverse effects in chickens. These results suggest that AC and HC mixed can reduced the aflatoxicosis in broilers and may be contribute to a solution of the aflatoxicosis problem in poultry production.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.3
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• pp.375-383
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• 2005

An experiment was conducted to study the protective role of polyherbal feed supplement (Growell) during induced mycotoxicosis in broilers. A total of 240 Vencobb broilers were divided at day old stage into eight equal groups. Group A served as control and was given plain feed, group B, D, F and H were given Growell at 0.35 g/kg of feed. Group C, D, G and H were given dietary aflatoxin $B_1$ at 0.2 ppm and groups E, F, G and H were given ochratoxin A at 0.2 ppm in feed to study effect of Growell on individual aflatoxicosis, ochratoxicosis and combined mycotoxicosis of broilers. The chicks were given their respective feeds from 1st day to 6th week of age and were vaccinated at 7th and 28th day of age with Lasota strain of Newcastle disease virus. There was no statistically significant effect of mycotoxins individually or in combination on body weight of broilers. However, body weights were highest in group B and lowest in co-mycotoxicated group G. Feed conversion ratio was best in group B followed by A, D, F, E, H and G. Significant improvement in haemoglobin values was observed in broilers due to feeding of Growell in ochratoxin and co-mycotoxicated groups. There was no significant effect of mycotoxin treatment on PCV, TEC and TLC of broilers. Due to single and combined mycotoxicosis, there was reduction in serum total protein, albumin, cholesterol and triglyceride and rise in alkaline phosphatase, creatinine and uric acid levels. Supplementation of diets with Growell reduced the alterations induced due to mycotoxins. There was a significant rise in per cent organ weight of liver and reduction of that of spleen, bursa of Fabricius and thymus of broilers fed mycotoxins. Protection from alteration in per cent organ weight of these organ by supplementation of Growell was recorded. The observed impaired immune response and histopathological changes in liver, kidney, spleen, bursa of Fabricius and thymus of broilers given mycotoxins were protected by supplementation of Growell.

• Korean Journal of Poultry Science
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• v.44 no.4
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• pp.283-290
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• 2017

Totally, one hundred and sixty 1-day-old Ross 308 broiler chicks were fed with a diet containing 0, 0.5, 1.0, and 2.0 mg of aflatoxin $B_1(AFB_1)/kg$ of feed for 21 days. Body weight was lower for the $AFB_1$-treated broilers than for the control group. At 14 and 21 DPF, the broilers fed with 2.0 mg of $AFB_1/kg$ of feed weighed significantly lower than those of the other groups (p<0.05). Relative liver weights increased significantly in a dose-dependent manner, and relative spleen weights were significantly high in the chicks fed with 2.0 mg of $AFB_1/kg$ of feed at 21 DPF (p<0.001). Biochemical analyses showed that total protein and albumin levels decreased significantly at 7 and 14 DPF for the chicks of the group fed with 2.0 of mg $AFB_1/kg$ of feed, compared with those fed with 0.5 and 1.0 mg of $AFB_1/kg$ of feed (p<0.05). AST and ALT levels increased significantly at 14 and 21 DPF (p<0.05), and the AST levels, particularly, increased dose-dependently (p<0.05). Histopathological analyses showed that the liver tissues of the $AFB_1$-treated chicks showed significant lesions, including hemorrhage, hepatocyte necrosis, inflammatory cell infiltration, and fatty degeneration. The severity of both hepatocyte necrosis and inflammatory cell infiltration appeared to increase dose- and time-dependently. Similarly, hepatic fibrosis increased dose-dependently (p<0.05). The results of this study could improve our understanding of parameters used for evaluating aflatoxicosis in poultry.

• Toxicological Research
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• v.17
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• pp.281-287
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• 2001

The Artichoke extract at 10% was used to add in drinking water to understand its effect on Aflatoxicosis of chickens. The Artichoke extract at the dose of 6 ml per liter of drinking water was given (experiment group) or not (control group) and to Hybro chickens (150 birds), during the first 49 days of life. Also, the chickens were fed with foodstuff containing 200 ppb or 500 ppb Aflatoxin $B_1$. Results showed that, the chickens having Artichoke extract: (1) Had overcome the growth retardation caused by the toxin at concentration of 200 ppb and 500 ppb of Aflatoxin $B_1$ (an addittonal weight gain of about 200-400 g/bird). (2) The feed conversion was improved (a reduction of 200-400 g of feed per kg of bird living weight). (3) Aflatoxicosis lesions were mild in the chickens, which fed 500 ppb of Aflatoxin $B_1$ or not found in those having the toxin 200 ppb. The blood examinations at 28th and 49th days of the trial gave the following results: (1) The Artichoke extract had an effect of suppressing the changes of blood cell numbers, hemoglobin amount. packed cell volume. leukocyte formula that were caused by Aflatoxin $B_1$. (2) The Artichoke extract had an effect of suppressing the diminution oj sugar, protein levels and the increase of the levels of GOT, GPT, alkaline phosphatase and bilirubin in the blood of intoxicated chickens. There was not or very Jew residue of Aflatoxin $B_1$ contained in the liver and muscle of chickens intoxicated by Aflatoxin $B_1$ having Artichoke, that was much lower than the allowed level in animal products.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.9
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• pp.1305-1309
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• 2005

Experiments were conducted to determine the efficacy of modified montmorillonite nanocomposite (MMN) to reduce the toxic effects of aflatoxin (AF) in growing/finishing pigs. 96 weaned pigs were assigned to four dietary treatment groups (0 g of MMN and 0 mg of AF/kg feed, 3 g of MMN/kg feed, 0.1 mg of AF/kg feed, and 3 g of MMN plus 0.1 mg of AF/kg feed). Body weight gain (BW gain), feed/gain ratio, serum biochemical values and enzyme activities were evaluated. Compared with the control, AF alone markedly reduced BW gain and resulted in a significantly higher feed/gain ratio. There were no differences in BW gain and feed/gain ratio between 0.3% MMN or 0.3% MMN plus AF and the control. These results suggested that the deleterious effects of AF were ameliorated by MMN addition. AF intake markedly increased relative organ weights of liver, kidney, spleen and pancreas, and resulted in significant alterations of serum parameters. However, these parameters for pigs fed diets containing MMN and AF returned to normal values, indicating that MMN had the ability to recover the AF-decreased performance, organ damage and to correct aberrations in serum parameters. These findings in our study suggested that MMN can effectively modulate the toxicity of AF in growing/finishing pigs and may offer a novel approach to the preventive management of aflatoxicosis in animals.

• Journal of Animal Science and Technology
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• v.58 no.7
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• pp.24.1-24.7
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• 2016

Background: Clinoptilolite is a natural zeolite with high adsorption capacity for polar mycotoxins such as aflatoxins. The efficacy of clinoptilolite in ameliorating the toxic effects of aflatoxicosis has been proven in monogastric animals, but there is no such evidence for ruminants. The aim of this study was to evaluate, under field conditions, whether the dietary administration of clinoptilolite in dairy cows could reduce the concentration of aflatoxin M1 ($AFM_1$) in bulk-tank milk, in farms with higher than or close to $0.05{\mu}g/kg$ of milk (European maximum allowed residual level). An objective of the present study was also to investigate the effect of particle size of clinoptilolite on aflatoxin binding. Methods: Fifteen commercial Greek dairy herds with AFM1 concentrations in bulk tank milk ${\geq}0.05{\mu}g/kg$ were selected. Bulk tank milk AFM1 was determined prior to the onset and on day 7 of the experiment. Clinoptilolite was added in the total mixed rations of all farms at the rate of 200 g per animal per day, throughout this period. Two different particle sizes of clinoptilolite were used; less than 0.15 mm in 9 farms (LC group) and less than 0.8 mm in 6 farms (HC group). Results: Clinoptilolite administration significantly reduced $AFM_1$ concentrations in milk in all farms tested at an average rate of 56.2 % (SD: 15.11). The mean milk $AFM_1$ concentration recorded on Day 7 was significantly (P < 0.001) lower compared to that of Day 0 ($0.036{\pm}0.0061$ vs. $0.078{\pm}0.0074{\mu}g/kg$). In LC group farms the reduction of milk $AFM_1$ concentration was significantly higher than HC group farms ($0.046{\pm}0.0074$ vs. $0.036{\pm}0.0061{\mu}g/kg$, P = 0.002). As indicated by the Pearson correlation, there was a significant and strong linear correlation among the milk $AFM_1$ concentrations on Days 0 and 7 (R = 0.95, P < 0.001). Conclusions: Dietary administration of clinoptilolite, especially of smallest particle size, at the rate of 200 g per cow per day can effectively reduce milk $AFM_1$ concentration in dairy cattle and can be used as a preventive measure for the amelioration of the risks associated with the presence of aflatoxins in the milk of dairy cows.

• Journal of Food Hygiene and Safety
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• v.15 no.1
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• pp.5-12
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• 2000

Lipid peroxidation is one of the main manifestations of oxidative damage and has been found play an important role in the toxicity and carcinogenesis of many carcinogens. This study was carried out to investigate the effects of aflatoxin B$_1$co-administrated with antioxidant vitamins on lipid contents and fatty acids components of liver in mice. For this work, vita-min C and vitamin E, the major antioxidants, were administrated with 10 mg/kg and 63.8 mg/kg respectively, through intraperitoneal(i.p) injection to male ICR mice, and 0.4 mg/kg of the AFB$_1$injected by i.p. 1hr later. The results were as follows: two fold amounts of free cholesterol, triglyceride, and total cholesterol in serum and liver of mice treated with only AFB$_1$were observed, when compared to those of mice co-administrated with antioxidant vitamins. However, the levels of phospholipids in serum and liver of mice treated with only AFB$_1$were decreased. Concerning to fatty acids composition of liver from AFB$_1$-treated mice, P/S ratio was shown more low level in cholesteryl ester, triglyceride, total cholesterol and phospholipid than those of mice co-administrated with antioxidant vitamins. In these data which provide with a reliable evidence on their antioxidantal effects to aflatoxicosis.