• Asian Pacific Journal of Cancer Prevention
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• v.14 no.9
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• pp.5231-5235
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• 2013

Objective: NF-E2-related factor 2 (Nrf2) is activated in several human malignancies. However, the role of Nrf2 in gastric cancer (GC) remains incompletely understood. In this study, we therefore analyzed associations of Nrf2 expression status with clinical features and chemotherapeutic resistance in GC. Materials and Methods: A total of 186 samples from GC patients who underwent gastrectomy were used for prognostic assessment. A further 142 samples from GC cases who received first-line combination chemotherapy were applied for investigation of chemoresistance. The Nrf2 expression was evaluated by immunohistochemistry in GC samples, and its relationship with clinicopathological parameters and chemotherapy sensitivity was analyzed. The effect of Nrf2 gene silencing on chemotherapy resistance was also examined by cell viability assay in vivo. Results: Of the 186 patients with GC, 104/186 (55.9%) showed high expression for Nrf2. The overexpression of Nrf2 was an independent predictor of overall survival [OS, hazard ratio (HR) 3.9; P=0.011] and disease-free survival (DFS, HR 4.3; P=0.002). The gene silencing of Nrf2 reduced resistance to cell death induced by 5-FU in GC cell lines. Conclusion: Our data show that Nrf2 is an independent prognostic factor in GC. Furthermore, Nrf2 confers resistance to chemotherapeutic drug 5-FU in GC cells. Taken together, Nrf2 is a potential prognostic marker and predictive for 5-FU resistance in GC.

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.723-729
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• 2003

Xenorhabdus nematophilus Kor-Al was cultured at flask and 5L jar fermentor at $28^{\circ}C$, 5% YS media condition. Antibiotic activity for X. nematophilus Kor-Al was experimented by paper disk method. As the result, antibiotic activity was growth associated form during culture time of X. nematophilus Kor-Al at flask. The maximum production and antibiotic activity were obtained at stationary period of cell growth. The optimum conditions of cell growth and antibiotic production at 5L jar fermentor were 400rpm agitation and 50% DO conditions.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.12
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• pp.6257-6261
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• 2012

Objective: To investigate the safety and efficacy of transcatheter arterial chemoembolization (TACE), combined with portal vein embolization (PVE), and high intensity focused ultrasound (HIFU) sequential therapy in treating patients with hepatocellular carcinoma (HCC). Methods: Patients with inoperative HCC were treated by two methods: in the study group with TACE first, then PVE a week later, and then TACE+PVE every two months as a cycle, after 2~3 cycles finally HIFU was given; in the control group only TACE+PVE was given. Response (CR+PR), and disease control rate (CR+PR+SD), side effects, overall survival and time to progress were calculated. Results: Main side effects of both groups were nausea and vomiting. No treatment related death occurred. In the study group, 32 patients received TACE for overall 67 times, PVE 64 times, and HIFU 99 times; on average 2.1, 2 and 3.1 times for each patient, respectively. In the control group, 36 patients were given TACE 78 times and PVE 74 times, averaging 2.2 and 2.1 times per patient. Effective rate: 25.0% in study group and 8.3% in control group (p>0.05). Disease control rates were 71.9% and 44.4%, respectively (p<0.05). In patients with portal vein tumor thrombus, the rate reduced over 1/2 after treatment was 69.2%(9/13) in the study and 21.4%(3/14) in the control group (p<0.05). Rate of AFP reversion or decrease over 1/2 was 66.7%(16/24) in study and 37%(10/27) (p<0.05) in control group. Median survival time: 16 months in study and 10 months in control group. PFS was 7months in study and 3 months in control group. Log-rank test suggested that statistically significant difference exists between two groups (p=0.024). 1-, 2- and 3-year survival rates were 56.3%, 18.8% and 9.3% in study, while 30.6%, 5.6% and 0 in control group, respectively, with statistically significant difference between two groups (by Log-rank, p = 0.014). Conclusions: The treatment of TACE+PVE+HIFU sequential therapy for HCC increases response rate, prolong survival, and could thus be a safe and effective treatment for advanced cases.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.5
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• pp.1803-1808
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• 2012

This study aimed to investigate the effects of Ser/Cys polymorphism in hOGG1 gene, Arg/Pro polymorphism in p53 gene, smoking and their interactions on the development of lung cancer. Ser/Cys polymorphism in hOGG1 and Arg/Pro polymorphism in p53 among 124 patients with lung cancer and 128 normal people were detected using PCR-RFLP. At the same time, smoking status was investigated between the two groups. Logistic regression was used to estimate the effects of Ser/Cys polymorphism and Arg/Pro polymorphisms, smoking and their interactions on the development of lung cancer. ORs (95% CI) of smoking, hOGG1 Cys/Cys and p53 Pro/Pro genotypes were 2.34 (1.41-3.88), 2.12 (1.03-4.39), and 2.12 (1.15-3.94), respectively. The interaction model of smoking and Cys/Cys was super-multiplicative or multiplicative, and the OR (95% CI) for their interaction item was 1.67 (0.36 -7.78). The interaction model of smoking and Pro/Pro was super-multiplicative with an OR (95%CI) of their interaction item of 5.03 (1.26-20.1). The interaction model of Pro/Pro and Cys/Cys was multiplicative and the OR (95%CI) of their interaction item was 0.99 (0.19-5.28). Smoking, hOGG1 Cys/Cys, p53 Pro/Pro and their interactions may be the important factors leading to the development of lung cancer.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.9
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• pp.5403-5408
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• 2013

Objective: CXCL12 exerts a wide variety of chemotactic effects on cells. Evidence indicates that CXCL12, in conjunction with its receptor, CXCR4, promotes invasion and metastasis of tumor cells. Our objective was to explore whether the CXCL12-CXCR4 biological axis might influence biological behavior of pancreatic cancer cells. Methods: Miapaca-2 human pancreatic cancer cells were cultured under three different conditions: normal medium (control), medium + recombinant CXCL12 (CXCL12 group), or medium + CXCR4-inhibitor AMD3100 (AMD3100 group). RT-PCR was applied to detect mRNA expression levels of CXCL12, CXCR4, matrix metalloproteinase 2 (MMP-2), MMP-9, and human urokinase plasminogen activator (uPA). Additionally, cell proliferation and invasion were performed using CCK-8 colorimetry and transwell invasion assays, respectively. Results: CXCL12 was not expressed in Miapaca-2 cells, but CXCR4 was detected, indicating that these cells are capable of receiving signals from CXCL12. Expression of extracellular matrix-degrading enzymes MMP-2, MMP-9, and uPA was upregulated in cells exposed to exogenous CXCL12 (P<0.05). Additionally, both proliferation and invasion of pancreatic cancer cells were enhanced in the presence of exogenous CXCL12, but AMD3100 intervention effectively inhibited these processes (P<0.05). Conclusions: The CXCL12-CXCR4 biological axis plays an important role in promoting proliferation and invasion of pancreatic cancer cells.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.9
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• pp.4773-4776
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• 2012

Objectives: To assess the efficacy, side effects, and the impact on quality of life with $Qinin^{(R)}$ (Cantharidin sodium) injection combined with chemotherapy for gastric cancer patients. Method: A consecutive cohort of 70 patients were divided into two groups: experimental group with cantharidin sodium injection combined with chemotherapy, while the control group received chemotherapy alone. After more than two courses of treatment, efficacy, quality of life and side effects were evaluated. Results: The response rate of experimental group was not significantly different from that of the control group (P>0.05), but differences were significant in clinical benefit response and KPS score. In addition, gastrointestinal reactions and the incidence of leukopenia were lower than in the control group (P<0.05). Conclusions: $Qinin^{(R)}$ (Cantharidin sodium) injection combined with chemotherapy enhances clinical benefit response, improving quality of life of gastric cancer patients and reducing side effects of chemotherapy. Thus $Qinin^{(R)}$ (Cantharidin sodium) injection deserves to be further investigated in randomized control clinical trails.

• Journal of the Korea Convergence Society
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• v.11 no.4
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• pp.203-219
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• 2020

This study attempts to empirically analyzes whether the establishment of a company-affiliated research institute affects corporate performance and innovation-oriented enterprises, and suggests implications for policy establishment. Logistic regression analysis was used to analyze if the establishment of an affiliated research institute and internal competency variables affect innovation and innovative performance-type companies. As a result, the establishment of an affiliated research institute was found to have a positive effect on the probability of belonging to an innovation-oriented company group, and it was found that it had a significant effect on business performance. In particular, there was significant effect on the production capability among internal competencies and the inclusion of innovative business groups is analyzed to have a mediating effect. Based on the research, the policy directions for encouraging the establishment of an affiliated research institute of small and medium-sized enterprises and revitalizing the research institute were suggested, and limitations and future research directions were suggested.

• Proceedings of the KIEE Conference
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• 2015.07a
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• pp.1525-1526
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• 2015

본 논문에서는 일반용 전기설비 안전점검으로 인한 전기화재 예방효과를 정량적으로 나타내기 위하여 전기화재 예방효과를 비용효과 분석방법을 통하여 정량적으로 제시하였다. 안전점검 실시유무에 따른 추상적인 분석모형을 개발하고, 개발된 분석모형을 정량적으로 나타내기 위한 수학적 논리를 이용한 수리모형으로 제안하고, 제안된 수리모형에 5년 동안에 일반용 전기설비 안전점검 실적과 화재 통계자료를 적용하여 전기안전점검에 대한 화재예방효과를 비용효과분석을 통하여 정량적으로 산출하였다.

• Proceedings of the KAIS Fall Conference
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• 2012.05b
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• pp.808-811
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• 2012

매년 높은 비율의 전기안전사고가 발생하지만 예방교육은 단순한 영상홍보나 단순 텍스트를 이용한 교육 수준에서 주입식으로 이루어지고 있다. 항공, 군사, 전력산업 등 다양한 분야에서는 가상현실을 이용한 체험형 교육 시스템이 개발되어 운영되지만 전기안전 예방교육은 감전사고와 같은 특수한 상황의 체험과 사고를 유발시킬 수 있다는 측면에서 체험형 교육시스템이 개발 및 적용이 어렵다. 따라서 본 연구에서는 전기감전 체험교육 시스템에 저주파 자극 장치를 사용할 수 있도록 저주파 간섭형 자극기를 이용한 인체 자극의 임계값 산출시험을 실시하고 대표적으로 성별, 연령별(아동 및 성인 구분)로 구분하여 적절한 자극값을 산출하였다. 본 연구의 결과는 향후 전기안전사고 예방을 위한 체험형 예방 교육 시스템에 활용이 가능할 것이다.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.6
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• pp.3855-3859
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• 2013

Purpose: Liver cancer, one of the most common cancers in China, is reported to feature relatively high morbidity and mortality. Curcumin (Cum) is considered as a drug possessing anti-angiogenic, anti-inflammation and anti-oxidation effect. Previous research has demonstrated antitumor effects in a series of cancers. Materials and Methods: In this study the in vitro cytotoxicity of Cum was measured by MTT assay and pro-apoptotic effects were assessed by DAPI staining and measurement of caspase-3 activity. In vivo anti-hepatoma efficacy of Cum was assessed with HepG2 xenografts. Results: It is found that Cum dose-dependently inhibited cell growth in HepG2 cells with activation of apoptosis. Moreover, Cum delayed the growth of liver cancer in a dose-dependent manner in nude mice. Conclusions: Cum might be a promising phytomedicine in cancer therapy and further efforts are needed to explore this therapeutic strategy.