• Title/Summary/Keyword: Aerobic activity

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Single Well Push-Pull Test를 이용한 TCE 오염 지하수의 In-Situ Bioremediation 타당성조사

  • Kim, Yeong;Istok, Jonnathan;Semprini, Lewis
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2003.04a
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    • pp.188-191
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    • 2003
  • Sing]e-well-push-pull tests were developed for use in assessing the feasibility of in-situ aerobic cometabolism of chlorinated aliphatic hydrocarbons (CAHs). The series includes Transport tests, Biostimulation tests, and Activity tests. Transport tests are conducted to evaluate the mobility of solutes used in subsequent tests. These included bromide or chloride (conservative tracers), propane (growth substrate), ethylene, propylene (CAH surrogates), dissolved oxygen (electron acceptor) and nitrate (a minor nutrient). Tests were conducted at an experimental well field of Oregon State University. At this site, extraction phase breakthrough curves for all solutes were similar, indicating apparent conservative transport of the dissolved gases and nitrate prior to biostimulation. Biostimulation tests were conducted to stimulate propane-utilizing activity of indigenous microorganisms and consisted of sequential injections of site groundwater containing dissolved propane and oxygen. Biostimulation was detected by the increase in rates of propane and oxygen utilization after each injection. Activity tests were conducted to quantify rates of substrate utilization and to confirm that CAH-transforming activity had been stimulated. In particular, the transformation of injected CAH surrogates ethylene and propylene to the cometabolic byproducts ethylene oxide and propylene oxide provided evidence that activity of the monooxygenase enzyme system, responsible for aerobic cometabolic transformations of CAHs had been stimulated. Estimated zero-order transformation rates decreased in the order propane > ethylene > propylene. The series of push-pu3l tests developed and field tested in this study should prove useful for conducting rapid, low-cost feasibility assessments for in situ aerobic cometabolism of CAHs.

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Effect for Wellness of Blood Flow Restriction Aerobic Exercise Program - Focusing on Mscle Ativity and Mtor Nurons - (혈류제한 유산소운동 프로그램의 웰니스를 위한 효과검정 - 근활성도와 운동신경원을 중심으로 -)

  • Jeong, Dae-Keun;Kang, Jeong-Il;Jang, Jun-Min
    • Journal of Korea Entertainment Industry Association
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    • v.15 no.7
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    • pp.225-233
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    • 2021
  • This study quantitatively compares and analyzes lower extremity muscle activity and motor neurons by performing blood flow-restricting aerobic training in the lower extremities, which is closely related to aerobic capacity for health, in normal people, and provides basic data to suggest the effectiveness of an effective blood-restricting exercise program. would like to provide A group of 10 people who applied aerobic exercise on a treadmill by restricting blood flow to 140 mmHg of pressure was set as Experimental Group I. And 11 people who applied only aerobic exercise on a treadmill were randomly assigned as a control group. The intervention program was implemented on a treadmill for 4 weeks, 3 times a week, once a day, for 30 minutes once. In addition, muscle activity and motor neurons were measured and analyzed using surface electromyography before intervention. As a result of the study, the muscle activity of the rectus femoris, biceps femoris, tibialis anterior and gastrocnemius was significantly increased (p<.001) in the pre-and-poster comparison within the group of experimental group I (p<.001). In the pre-and-poster comparison of the control group, the muscle activity of the rectus femoris, biceps femoris, tibialis anterior and gastrocnemius was significantly increased (p<.001). In comparison of changes between groups, there was a significant difference in the activity of the rectus femoris muscle (p<.05). Combining aerobic exercise in parallel with lower extremity blood flow restriction can be developed into an injury prevention exercise program that can restore functional activity in rehabilitation training for elite athletes and elderly people with weak joints. In addition, based on these results in future research, it is considered that it is necessary to expand the scope of non-normal subjects and conduct various studies according to the pressure intensity.

Green Tea Maintains Antioxidative Defense Enzyme Activities and Protects Against Lipid Peroxidation in Rat Gastrocnemius Muscles After Aerobic Exercise

  • Chai, Young-Mi;Rhee, Soon-Jae
    • Preventive Nutrition and Food Science
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    • v.8 no.4
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    • pp.377-382
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    • 2003
  • This study investigated the effects of green tea on the muscle antioxidative defense system in the white & red gastrocnemius muscles of rats after aerobic exercise. Male Sprague-Dawley rats weighing 150 10 g were randomly assigned to a control group, non-exercise with green tea group (G group), and exercise training group. The exercise training group was then further classified as the training (T) group and training with green tea (TG) group, the latter of which was supplemented with green tea in the drinking water during the experimental period. The rats in the exercise training groups (T and TG) were subjected to aerobic exercise on a treadmill 30 min/day at a speed of 28 m/min (7% incline) 5 days/week, while the other groups (control and G group) were cage confined for 4 weeks. Thereafter, the rats were sacrificed with an injected overdose of pentobarbital just after running. In the white muscle, the xanthine oxidase (XOD) activities were 71 % higher in the T group compared to control group, whereas the TG group had the same activity as the control group. The XOD activities in the red gastrocnemius muscle exhibited the same tendency as in the white muscle. The superoxide dismutase (SOD) activity in the white muscle was lower in the T group compared with the control group, yet significantly higher in the TG group compared with the T group. The SOD activities in the red gastrocnemius muscle exhibited the same tendency as in the white gastrocnemius muscle. The glutathione peroxidase (GSHpx) activities in the white & red gastrocnemius muscles were 43 % lower in the T group compared with the control group, yet the activities in the TG group remained at control levels. The glutathione S-transferase (GST) activity in the white muscle was not significantly different among any of the three groups, but in the red gastrocnemius muscle, the TG group had the same activity as in the control group. The thiobarbituric acid reactive substance (TBARS) contents in the white & red gastrocnemius muscles were higher in the T group than in the control but the control and TG groups had the same concentrations of TBARS. In conclusion, the supplementation of green tea in rats subjected to aerobic exercise was found to reduce the peroxidation of muscle lipids by enhancing the antioxidative defense mechanism.

Computational identification of significantly regulated metabolic reactions by integration of data on enzyme activity and gene expression

  • Nam, Ho-Jung;Ryu, Tae-Woo;Lee, Ki-Young;Kim, Sang-Woo;Lee, Do-Heon
    • BMB Reports
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    • v.41 no.8
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    • pp.609-614
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    • 2008
  • The concentrations and catalytic activities of enzymes control metabolic rates. Previous studies have focused on enzyme concentrations because there are no genome-wide techniques used for the measurement of enzyme activity. We propose a method for evaluating the significance of enzyme activity by integrating metabolic network topologies and genome-wide microarray gene expression profiles. We quantified the enzymatic activity of reactions and report the 388 significant reactions in five perturbation datasets. For the 388 enzymatic reactions, we identified 70 that were significantly regulated (P-value < 0.001). Thirty-one of these reactions were part of anaerobic metabolism, 23 were part of low-pH aerobic metabolism, 8 were part of high-pH anaerobic metabolism, 3 were part of low-pH aerobic reactions, and 5 were part of high-pH anaerobic metabolism.

The Effect of Aerobic Exercise and Allium Tuberosum Intake on Blood Lipids, MDA and Antioxidant Enzyme in Rats (유산소 운동과 부추섭취가 혈중지질, 지질과산화 및 항산화효소에 미치는 영향)

  • Baek, Yeong-Ho;Lee, Sang-Ho
    • Journal of Life Science
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    • v.20 no.2
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    • pp.245-252
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    • 2010
  • The purpose of this study was to investigate the effects of aerobic exercise and Allium tuberosum intake on blood lipids, MDA and antioxidant enzyme in rats. Twenty four male Sprague-Dawley rats, 4 weeks old, were used. Experimental groups were aerobic exercise with Allium tuberosum intake group (A, n=6), aerobic exercise group (B, n=6), Allium tuberosum intake group (C, n=6), and the control group (D, n=6). Aerobic exercise was administered through a treadmill running program (14~15 m/min, $0^{\circ}$ grade, 25~30 min/day, 5 day/wk) and these rats were given 5% Allium tuberosum for 2 wk. The results of this study are as follows: TC and TG didn't show change; groups A, B, C showed a significant increase in HDL-C compared to the D group; groups A, B, and C showed a significant decrease in LDL-C compared to the D group; groups B and C showed a significant decrease in MDA level compared to the D group; groups B and C showed a significant increase in SOD activity compared to the D group; and the A group showed a significant increase in CAT activity compared to the D group. In conclusion, low intensity aerobic exercise and intake of the natural antioxidant Allium tuberosum seem to have the health promoting effect of retarding oxidative stress by decreasing lipid peroxidation.

Studies on the Microflora and Enzyme Activity in Processing of Accelerated Low Salt-Fermented Anchovy by adding koji (코오지를 이용한 속성 저식염 멸치젓의 미생물상과 효소활성)

  • 백승화;임미선;김동한
    • The Korean Journal of Food And Nutrition
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    • v.9 no.4
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    • pp.392-397
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    • 1996
  • To produce low salt fermented anchovy by an accelerated method with Asp. oryzae and Bacillus sp. koji, enzyme activity and variation of microflora during the 60 day fermentation were examined. Bacterial counts changed a little during the fermentation with the highest on day 40 for proteolytic and anaerobic bacteria and on day 20 for aerobic bacteria. Proteolytic, lipolytic, aerobic, and anaerobic bacteria counts were higher in the Bacillus sp. koji added anchovy paste than in others. The protease and lipase activities reached the highest point on day 20 and 30, respectively, and decreased gradually afterwards. The protease activity was higher in Asp. oryzae koji than in bacillus sp. koji, but the lipase activity was to the contrary.

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A study on the isozyme alterations of lactic dehydrogenase in the tissues of albino rat by the exposure in sulfur dioxide (아황산가스에 노출된 백서조직 lactic dehydrogenase의 isozyme변화에 관한 연구)

  • 권숙표
    • YAKHAK HOEJI
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    • v.13 no.4
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    • pp.101-110
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    • 1969
  • The isozyme alteration of lactic dehydrogenase in the tissues of albino rat inhaled SO$_{2}$ were studied in vivo and in vitro, with the following results: (1) The H-type of LDH activity relatively dominated in the normal brain, heart and kidney tissues of rat, M-type in the normal lung, liver, and muscle tissues of the animal. (2) When rats inhale SO$_{2}$ in the concentration of 250 ppm, it appears that the M-type tends to predominate in the anaerobic tissues such as liver, kidney and muscle tissues and the H-type in the aerobic tissues such as brain and heart tissues. (3) When 5% SO$_{2}$ is introduced into tissue homogenates, LDH activities in the heart, lung, liver and muscle tissues are increased more than that of introducing room-air only. With sam treatment, LDH activity is decreased in the kidney tissue and no alteration is observed in the brain tissue. (4) Although, after the aeration of SO$_{2}$, the oxygen tension seems to bring decreases in the level of LDH activity in the anerobic tissues such as liver and muscle tissues, while, on the other hand, increases in the level of the activity in the aerobic tissues, such as the brain, heart and lung tissues. (5) Accordinglly, SO$_{2}$ affects LDH activities, its isozyme pattern of each organs, and their metabolic pathway by its absorption of the gas.

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Effect of DO Concentration on Ammonia Oxidizing Bacteria in Aerobic Biofilm Reactor (호기성 생물막 반응기에서 Ammonia Oxidizing Bacteria에 대한 DO 농도의 영향)

  • Yu, Jae-Cheul;Park, Jeung-Jin;Hur, Sung-Ho;Kim, Yu-Jin;Byun, Im-Gyu;Lee, Tae-Ho;Park, Tae-Joo
    • Journal of Korean Society of Environmental Engineers
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    • v.29 no.1
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    • pp.106-112
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    • 2007
  • Ammonia oxidizing bacteria(AOB) oxidize ammonia to nitrite and are important microorganisms which control nitrification. Several environmental factors such as dissolved oxygen(DO), temperature and pH influence the growth of AOB. In this work, to assess the effect of DO concentration on AOB, four aerobic biofilm reactors packed with ceramic media were operated 1, 3, 5 and 7 mgDO/L, respectively. The optimal DO concentration with stable nitrification efficiency in aerobic biofilm reactor was above 5.0 mg/L. To assess the relationship between the DO concentration and the characteristics of AOB in aerobic biofiim reactor, DGGE and cloning based on PCR targeting 16S rRNA and amoA gene were performed. Additionally, INT-DHA activity test was proceeded to estimate the activity of AOB. As the results of DGCE and cloning, the community of AOB and the ratio of Nitrosomonas sp. changed little in spite of different nitrification efficiencies. INT-DHA activity test showed that the activity of AOB decreased as DO concentration decreased. It means that DO concentration does not affect the community of AOB, but the activity of AOB.

A Study on the Weight Change and Exercise Immersion for Activation of Aerobic Dance (에어로빅댄스 활성화를 위한 체중변화와 운동몰입에 관한 연구)

  • Lee, Seung-Do;Kim, Kyoung-Hyun
    • Journal of Korea Entertainment Industry Association
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    • v.14 no.5
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    • pp.115-130
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    • 2020
  • The purpose of this study is to analyze the difference between A Study on the Weight Change and Exercise Immersion for Activation of Aerobic Dance and to suggest the continuous activation of Aerobic Dance. To achieve this purpose, a questionnaire was conducted using a convenience sampling method, which is one of non-probability sampling, in April 2020, targeting general aerobic dance members in Gyeongnam. The results of this study are as follows. First, the demographic characteristics were partially different. The age was 40 to 49 years, height was more than 160cm, occupation was housewives, weight change was more than -3kg, participation intensity was more than 100 minutes, participation frequency was 4 days, and participation period was less than 5 years. Second, the results of this study are as follows: Weight Change(Body Confidence, Activity Confidence, Dance Confidence, Psychological Stability Effect, Dance Activity Effect, Perform-motion Effect) and Exercise Immersion(Behavior immersion, Cognitive immersion) were partially high There was a difference. Third, the results of this study are as follows: Weight Change(Body Confidence, Activity Confidence, Dance Confidence, Psychological Stability Effect, Dance Activity Effect, Perform-motion Effect) and Exercise Immersion(Behavior immersion, Cognitive immersion) were partially high It was shown that the influence was.

Isolation of the Component transformed into Blue Pigments by Aerobic Bacteria in the Fruits of Gardenia jasminoides (치자중 호기성 세균에 의해 청색색소로 변환되는 성분의 단리)

  • Park, Chang-Hun;Kang, So-Im;Min, Eung-Gi;Han, Yeong-Hwan;Lee, Chung-Kyu;Lee, Dong-Ung
    • Korean Journal of Pharmacognosy
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    • v.29 no.3
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    • pp.204-208
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    • 1998
  • Geniposide, an iridoid glucoside, has been isolated from the butanol fraction of Gardenia jasminoides Ellis (Rubiaceae). The component was found to be transformed into the blue pigments by some aerobic bacteria, suggesting that geniposide is the precursor for the formation of pigments after converting into genipin, an aglycon of geniposide, by ${\beta}-glucosidase$. Some bacteria having a ${\beta}-glucosidase$ activity did not form the pigments, which may mean that the formation of pigments can only be occurred by the reaction of any enzyme or compound in the pigment-producing bacteria.

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