• Journal of Life Science
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• v.29 no.1
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• pp.45-51
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• 2019

This study is to investigate the effects of the physical activity and intake of improved food levels on male body compositions. Forty men were assigned to a percentage of body fat (PBF) group (20 men with physical exercise and food) and CONTROL group (20 men with exercise, food intake is free) for this six-month study. An aerobic capacity test to measure maximum oxygen consumption ($VO_2$ max) was performed to precisely determine the exercise intensities during the experimental conditions. After exercise and food control, the body weight of 20s with exercise and food (A) and only exercise (B) decreased about 2.3 kg and 0.4 kg after 6 months, respectively. If the p-values were 0.697(between baseline and 3 months), 0.535(between 3 months and 6 months), and 0.617(between baseline and 6 months), respectively, then 20s men had evidence of that a significant main effect does not exist amongst the observations of the outcome or within-weights. Their skeletal muscle mass (SMM) of both groups increased slightly through exercise. Although the decrease in total body weight of 30s was correlated with the increase in SMM, there was not shown significant. While total body water decreased in 20s, 40s, and 50s except 30s men, all other values did not shown significant differences. The physical activity and intake of improved food levels were contributed with lost a lot of weight, decrease of SMM and body fat.

• Microbiology and Biotechnology Letters
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• v.49 no.3
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• pp.329-336
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• 2021

Cellulophga sp. J9-3, is a gram-negative, aerobic marine bacterium belonging to the family Flavobacteriaceae. In addition to cellulose degradability, the J9-3 strain is also capable of hydrolyzing agar in the solid and liquid medium, and the production of agarase in the presence of agarose can be remarkably induced by the bacterium. From the cell culture broth of Cellulophga sp. J9-3, ammonium sulfate precipitation and three kinds of column chromatography were successively performed to purify a specific agarase protein, the AgaJ93. Purified AgaJ93 showed the strongest hydrolyzing activity towards agarose (approximately 22%), and even displayed activity towards starch. AgaJ93 hydrolyzed agarose into neoagarotetraose and neoagarohexaose via various oligosaccharide intermediates, indicating that AgaJ93 is an endo-type β-agarase. AgaJ93 showed maximum activity at a pH of 7.0 and temperature of 35 ℃. Its activity increased by more than six times in the presence of Co²⁺ ions. The N-terminal sequence of AgaJ93 showed 82% homology with the heat-resistant endo-type β-agarase Aga2 of Cellulophaga sp. W5C. However, the biochemical properties of the two enzymes were different. Therefore, AgaJ93 is expected to be a novel agarose, different from the previously reported β-agarases.

• Journal of Microbiology and Biotechnology
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• v.31 no.9
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• pp.1210-1217
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• 2021

Two gram-negative, catalase-positive, strictly aerobic, and white colony-forming bacteria, strains H242^T and B156^T, were isolated from soil in South Korea. Cells of strain H242^T were oxidase-positive and non-motile short rods, while those of strain B156^T were oxidase-negative and long non-motile rods. Ubiquinone-8 was identified as the sole isoprenoid quinone in both strains. C_16:0, cyclo-C_17:0, andsummed feature 3 (C_16:1 ω7c and/or C_16:1 ω6c) and phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol were identified in both strains as the major cellular fatty acids and polar lipids, respectively. The DNA G+C contents of strains H242^T and B156^T were 69.4 mol% and 69.3 mol%, respectively. Phylogenetic analyses based on 16S rRNA and 92 concatenated core gene sequences revealed that strains H242^T and B156^T formed distinct phylogenic lineages from other Ramlibacter type strains. The DNA-DNA hybridization (DDH) value between strains H242^T and B156^T was 24.6%. Strains H242^T and B156^T were most closely related to Ramlibacter ginsenosidimutans BXN5-27^T and Ramlibacter monticola G-3-2^T with 98.4% and 98.6% 16S rRNA gene sequence similarities, respectively. Digital DDH values between strain H242^T and R. ginsenosidimutans and between strain B156^T and R. monticola were 23.5% and 26.1%, respectively. Phenotypic, chemotaxonomic, and molecular analyses indicated that strains H242^T and B156^T represent two novel species of the genus Ramlibacter, for which the names Ramlibacter terrae sp. nov. and Ramlibacter montanisoli sp. nov., respectively, are proposed. The type strains of R. terrae and R. montanisoli are H242^T (=KACC 21667^T=JCM 33922^T) and B156^T (=KACC 21665^T=JCM 33920^T), respectively.

• Journal of Korean Society on Water Environment
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• v.27 no.3
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• pp.322-328
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• 2011

This study was performed to investigate the characteristics of nutrient removal of municipal wastewater in the membrane bioreactor system with the different types of membrane. Membrane bioreactor consists of three reactors such as two intermittent anaerobic and the submerged membrane aerobic reactor with flat sheet and hollow fiber membrane, respectively. The removal efficiencies of $COD_{cr}$, BOD, SS, TN and TP on the flat sheet membrane bioreactor were 94.3%, 99.0%, 99.9%, 70.3% and 63.1%, respectively. In addition, The removal efficiencies of $COD_{cr}$, BOD, SS, TN and TP on the hollow fiber membrane bioreactor were 94.0%, 99.3%, 99.9%, 69.9% and 66.9%, respectively. The estimated true biomass yield, specific denitrification rate (SDNR), specific nitrification rate (SNR) and phosphorus removal content on the flat sheet membrane bioreactor were $0.33kgVSS/kgBOD{\cdot}d$, $0.043mgNO_3-N/mgVSS{\cdot}d$, $0.031mgNH_4-N/mgVSS{\cdot}d$, and 0.144 kgP/d, respectively. In addition, the estimated true biomass yield, specific denitrification rate (SDNR), specific nitrification rate (SNR) and phosphorus removal content on the hollow fiber membrane bioreactor were $0.30kgVSS/kgBOD{\cdot}d$, $0.067mgNO_3-N/mgVSS{\cdot}d$, $0.028mgNH_4-N/mgVSS{\cdot}d$, and 0.121 kgP/d, respectively. There was little difference between the flat sheet and hollow fiber on the nutrient removal efficiencies except SNR and SDNR. These differences between them were caused by the air demand to prevent the membrane fouling. The flux and oxygen demand for air scouring were $19.0L/m^2/hr$ and $2.28m^3/min$ for the flat sheet membrane, and $20.7L/m^2/hr$ and $1.77m^3/min$ for the hollow fiber membrane on an average.

• Food Engineering Progress
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• v.22 no.4
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• pp.386-391
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• 2018

Effects of a commercial scale intervention system combining ultraviolet (UV)-C and plasma treatments on the microbial decontamination of black pepper powder were investigated. The process parameters include treatment time, time for plasma accumulation before treatment, and water activity of black pepper powder. A significant reduction in the number of indigenous aerobic mesophilic bacteria in black pepper powder was observed after treatments lasted for ${\geq}20min$ (p<0.05) and the reduction was differed by powder manufacturer. The microbial reduction rates obtained by individual UV-C treatment, individual plasma treatment, and UV-C/plasma-combined treatment were 0.2, 0.5, and 1.0 log CFU/g, respectively, suggesting that the efficacy of the microbial inactivation was enhanced by treatment combination. Nonetheless, neither plasma accumulation time nor powder water activity affected the microbial inactivation efficacy of the combined treatment. The UV-C/plasma-combined treatment, however, decreased lightness of black pepper powder, and the decrease generally increased as operation time increased. The plasma accumulation time of 20 min resulted in significant reduction in both lightness and brown color. The results indicate that the commercial-scale intervention system combining treatments of UV-C and plasma has the potential to be applied in the food industry for decontaminating black pepper powder.

• Journal of Life Science
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• v.31 no.5
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• pp.464-474
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• 2021

Inflammation induced by metabolic syndromes, cancers, injuries, and sepsis can alter cellular metabolism by reducing mitochondrial function via oxidative stress, thereby resulting in neuropathy and muscle atrophy. In this study, we investigated whether butyrate, a short chain fatty acid produced by gut microbiota, could prevent mitochondrial dysfunction and muscle atrophy induced by lipopolysaccharide (LPS) in the C2C12 cell line. LPS-activated MAPK signaling pathways increased the levels of the mitochondrial fission signal, p-DRP1 (Ser616), and the muscle atrophy marker, atrogin 1. Interestingly, butyrate significantly inhibited the phosphorylation of JNK and p38 and reduced the atrogin 1 level in LPS-treated C2C12 cells while increasing the phosphorylation of DRP1 (Ser637) and levels of mitofusin2, which are both mitochondrial fusion markers. Next, we investigated the effect of MAPK inhibitors, finding that butyrate had the same effect as JNK inhibition in C2C12 cells. Also, butyrate inhibited the LPS-induced expression of pyruvate dehydrogenase kinase 4 (PDK4), resulting in decreased PDHE1α phosphorylation and lactate production, suggesting that butyrate shifted glucose metabolism from aerobic glycolysis to oxidative phosphorylation. Finally, we found that these effects of butyrate on LPS-induced mitochondrial dysfunction were caused by its antioxidant effects. Thus, our findings demonstrate that butyrate prevents LPS-induced muscle atrophy by improving mitochondrial dynamics and metabolic stress via the inhibition of JNK phosphorylation. Consequently, butyrate could be used to improve LPS-induced mitochondrial dysfunction and myopathy in sepsis.

• Journal of Wetlands Research
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• v.23 no.2
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• pp.183-188
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• 2021

In the case of small-scale sewage treatment plants, it is reported that the amount of inflow fluctuates and it is difficult to operate the sewage treatment due to the inflow of unknown water due to the aging of sewage pipes. In particular, there are many overall operational problems due to the decrease in water temperature in winter. In this study, the operation status of small-scale sewage treatment facilities located in mountainous areas and water quality changes according to temperature were analyzed. It was found that the concentration of BOD, COD, and SS in effluent water was greatly changed depending on the temperature, and it was found that COD was particularly affected. Accordingly, the water level of the bioreactor was raised by 0.4m in order to temporarily apply measures to lower the water temperature in winter. As a result of comparing and analyzing the results when the bioreactor was covered and operated, a significant improvement effect occurred. In addition, a plan to improve the treatment efficiency of the bioreactor in winter is to extend the residence time of the bioreactor, a plan to expand the bioreactor specification, a new flow control tank and transport it to the outside, and an oxygen-free air diffuser to be used as an aerobic tank in case of an emergency in winter. The improvement plan was suggested. The results of this study are expected to be used as basic data for the operation plan of small-scale sewage treatment facilities in winter.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.2
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• pp.368-377
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• 2021

Plum is a typical fruit that is consumed processed rather than raw. In this study, we manufactured one of the processed foods, viz., plum Jangachi. In this process, the manpower-dependent seed separation and flesh cutting operations were automated by mechanizing, thereby altering the manufacturing process. Quality and Safety were assessed through microbial evaluation, analysis of color, and detection of preservatives in the plum Jangachi. Preference factors were identified through sensual evaluation. When compared with other plum Jangachi currently available in the market, our product was determined to contain 2.7±0.1 Log CFU/g total aerobic bacteria, which is slightly higher than the average of other products. This was not surprising, as the figures are due to the inherent characteristics, which were determined to be lower as compared to other commercial plum Jangachi. Other coliforms, tar dyes, and preservatives were undetected, thereby conferring satisfactory Quality and Safety. In general, there was no statistical difference in the sensual evaluation and appearance; overall, our product received better feedbacks than products on the market. Taken together, our results provide a foundation for applying the mechanization of plum-processed foods, thereby promoting the local economy in the main production area, and overall characteristics obtained are regarded sufficient in terms of market competitiveness.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.12
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• pp.162-169
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• 2020

This study was conducted from November 25 to December 4, 2019, to unmask differences in physical fitness based on the weight class of high school Ssirum elite-players. A total of 28 high school Ssirum elite-players were compared and analyzed by measuring physical factors (muscle strength/power, agility, anaerobic power, aerobic ability, flexibility, and isokinetic muscle function) by weight class. Data were analyzed using SPSS ver. 23.0. One-way ANOVA was used for comparison between groups. There was no difference by weight class in terms of grip strength, sit-ups, sergeant jump, long jump, flexibility, side step, and reaction time. Evaluation of back muscle strength revealed that the group with more than 100 kg had greater strength than lower weight categories, whereas it was lower in the sit-ups, 20 m shuttle run and the relative values anaerobic power. Considering isokinetic muscle function, no difference was observed between the groups of back muscle strength and knee joint. The results of this study indicate that amongst the high school Ssirum elite-players, there is no difference in muscle power, flexibility, agility, and anaerobic average power per body weight, isokinetic trunk strength, and knee flexion strength, when evaluated according to weight class.

• The Journal of the Convergence on Culture Technology
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• v.7 no.3
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• pp.293-301
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• 2021

This study was to find out the influence of urine cotinine in Korean adults on Cardiovasculardiseases using data from the seventh Korea national health and nutrition examination survey 2016-2018. The subjects were 7,290. The data were analyzed by frequency analysis, 𝞆2 test and logistic regression analysis using SPSS 26. The main findings were that 40-59 age, higher than 60 age, lower than high school level, Income status low, urine cotinine level under 50ng/mL, urine cotinine level 50-499ng/mL, Average cigarette per day over the 20 loosey, BMI 25g/m², no aerobic exercise, the prevalence of cardiovascular disease increased 9.0 times, 29.44 times,1.26 times, 1.68 times, 1.54 times, 1.56 times, 1.48 times, 2.06 times and 1.17 times, respectively. As a result, it is necessary to improve lifestyles such as obesity and lack of exercise, to reduce the prevalence of cardiovascular disease in Korean adults, and to pay attention to older age groups and low socioeconomic groups.