• Journal of Microbiology and Biotechnology
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• v.17 no.5
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• pp.784-791
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• 2007

The present work aims to use a biofilter technology(aerated submerged filters) for the aerobic transformation at laboratory-scale of olive washing water(OWW) generated in the first steps of olive oil processing, as well as the genetic profiling and identification to the species level of the bacteria involved in the formation of the biofilm, by means of TGGE. Chemical parameters, such as biological oxygen demand at five days($BOD_5$) and chemical oxygen demand(COD), decreased markedly(up to 90 and 85%, respectively) by the biological treatment, and the efficiency of the process was significantly affected by aeration and inlet flow rates. The total polyphenol content of inlet OWW was only moderately reduced(around 50% decrease of the inlet content) after the biofilter treatment, under the conditions tested. Partial 16S rRNA genes were amplified using total DNA extracted from the biofilm and separated by TGGE. Sequences of isolated bands were mostly affiliated to the $\alpha-subclass$ of Proteobacteria, and often branched in the periphery of bacteria] genera commonly present in soil(Rhizobium, Reichenowia, Agrobacterium, and Sphingomonas). The data obtained by the experimentation at laboratory scale provided results that support the suitability of the submerged filter technology for the treatment of olive washing waters with the purpose of its reutilization.

• Korean Journal of Soil Science and Fertilizer
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• v.40 no.2
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• pp.156-163
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• 2007

This study was conducted to evaluate the liquefying efficiency of mixed organic fertilizer in different conditions. The organic fertilizer was composed of sesame oil cake, rice bran, fish meal, ground bone meal etc, and made by fermenting process. It included $23g\;kg^{-1}$, $17.0g\;kg^{-1}$, $23.9g\;kg^{-1}$, $290g\;kg^{-1}$ of N, $P_2O_5$, $K_2O$, organic matter, respectively. In one test, the mixed organic fertilizer was added in the proportion of 10% to water 90% and aerated continuously, for 2, 8 hours per day, and not aerated as control. In the other test, ratios of organic fertilizer to water were 5%, 10%, 20% and aerated for 2 hours a day. With the increase of liquefying time, pH, EC and $NH_4-N$ increased without relation to aeration time. After 10 days, liquid organic fertilizer aerated for 2 hours a day contained $634mg\;N\;kg^{-1}$, $68.1mg\;P_2O_5\;kg^{-1}$, $453mg\;K_2O\;kg^{-1}$, which was not significantly different from 8 hours a day or continuous aeration. Then extraction ratios of inorganic contents were 27.6%, 4.0% and 18.9%, respectively. Continuous aeration resulted in increasing the viable number of aerobic bacteria, spore forming bacteria and fungi in liquefied solution. Higher ratio of organic fertilizer to water increased EC, $NH_4-N$ and other inorganic matter contents, but decreased extraction ratio of nutrients in liquid fertilizer. The liquid organic fertilizer of 20% contained $1,140mg\;N\;kg^{-1}$, $35.4mg\;P_2O_5\;kg^{-1}$, $544mg\;K_2O\;kg^{-1}$ after 10 days. Then extraction ratios were 24.8%, 2.4% and 13.6%, respectively. The ratio of organic fertilizer to water was positively correlated with only spore forming bacteria, Pseudomonas spp. among microorganisms.

• Applied Biological Chemistry
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• v.13 no.1
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• pp.43-50
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• 1970

To study the productivity of single cell protein from the petroleum hydrocarbon utilizing yeasts, 242 soil samples, such as oil soaked soil of gas stations and garage, coal, farm soil, and sewage, from 135 places in Korea were collected. From these samples 468 yeast strains which utilize petroleum hydrocarbon as a sole organic carbon source were isolated and identified by observing the growth rates. For the identified strains optimum culture conditions were determined and analysis of cell components were performed. 1. 90.8% of petroleum hydrocarbon utilizing yeast strains were found from oil soaked soil and about 10% from coal, farm soil and sewage etc. 2. The yeast strain of the highest cell productivity was isolated from oil soaked soil and was identified as Candida curvata HY-69-19. 3. The optimum culture conditions for the selected yeast strain were found to be pH 5.0, $28^{\circ}C$ and affluent aerated state. 4. Candida curvata HY-69-19 was found to utilize favorably the heavy gas oil fractionated at above $268.9^{\circ}C$ as carbon source and urea as inorganic nitrogen source. 5. The growth curve of this strain on heavy gas oil medium showed that the yeast has a lag phase up to 18 hours and logarithmic growth phase between 24 to 42 hours. Generation time was found to be between 3.8 and 4.5 hours during the logarithmic growth phase. 6. About 300 mg dried cells per heavy gas oil was harvested under the culture conditions of adjusted pH to 5.0 at time intervals of 6 hours for 54 hours and heavy gas oil urea for shaking culture medium. 7. Chemical composition of the yeast cell was found to be 40.25%, 14.81%, 24.32% and 10.63% for crude protein, crude lipid, carbohydrate and ashes, respectively.