• Journal of Microbiology and Biotechnology
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• 제16권9호
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• pp.1347-1354
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• 2006

The aims of this study were to establish a simple and effective method for isolating male germline stem cells (GSCs), and to test the possibility of using these cells as a new approach for male infertility treatment. Testes obtained from neonatal and adult mice were manually decapsulated. GSCs were collected from seminiferous tubules by a two-step enzyme digestion method and plated on gelatin-coated dishes. Over 5-7 days of culture, GSCs obtained from neonates and adults gave rise to large multicellular colonies that were subsequently grown for 10 passages. During in vitro proliferation, oct-4 and two immunological markers (Integrin ${\beta}1,\;{\alpha}6$) for GSCs were highly expressed in the cell colonies. During another culture period of 6 weeks to differentiate to later stage germ cells, the expression of oct-4 mRNA decreased in GSCs and Sertoli cells encapsulated with calcium alginate, but the expression of c-kit and testis-specific histone protein 2B(TH2B) mRNA as well as the localization of c-kit protein was increased. Expression of transition protein (TP-l) and localization of peanut agglutinin were not seen until 3 weeks after culturing, and appeared by 6 weeks of culture. The putative spermatids derived from GSCs supported embryonic development up to the blastocyst stage with normal chromosomal ploidy after chemical activation. Thus, GSCs isolated from neonatal and adult mouse testes were able to be maintained and proliferated in our simple culture conditions. These GSCs have the potential to differentiate into haploid germ cells during another long-term culture.

• 산업융합연구
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• 제21권9호
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• pp.89-102
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• 2023

본 연구는 성인간호학 교과목에 적용한 사례기반 문제중심학습(PBL) 수업 후 간호대학생의 메타인지, 문제해결능력, 전문직 자아개념 및 자기주도학습능력에 미치는 효과를 확인하기 위한 단일군 사전사후설계(One group pre-post test design) 실험연구이다. 연구대상은 D광역시 D간호대학의 성인간호학 교과목을 수강 신청한 4학년 60명이었으며, 2022년 9월부터 12월까지 자료를 수집하였다. 성인간호학 수업 모형은 PBL에 적합한 ADDIE모형을 기반으로 설계하였다. 교수학습 과정은 총 15주이었으며, 8주는 강의, 2주는 지필고사, 5주는 Barrow와 Myers의 5단계 PBL학습으로 진행하였다. 수집된 자료는 SPSS/WIN 20.0 Program을 사용하여 분석하였고 중재 전 후 변수 간의 차이 검정은 Paired t-test를 이용하였다. PBL수업 전·후 간호대학생의 메타인지(t=-8.04, p<.001), 문제해결능력(t=-4.08, p<.001), 전문직 자아개념(t=-4.67, p<.001) 및 자기주도학습(t=-4.65, p<.001)는 유의미한 차이를 나타냈다. 그러므로 간호학생들의 메타인지, 문제해결, 전문 직 자아개념 및 자기주도학습능력을 강화시키기 위해 간호학의 여러 전공교과목에서 PBL을 적용할 것을 제안한다.

• The Journal of Korean Physical Therapy
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• 제1권1호
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• pp.63-72
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• 1989

The present study was designed to investigate the general characteristics of pain patients and to analyze the properties of Korean pain expression terms as a preliminary step in the development of a pain questionnaire. Questionnaires were administered to 73 adult patients (53 males, 20 females) with knee, ankle, neck, low back, and shoulder pain. The mean duration of pain was 16.2 months (SE=3.3). The results were as fellows : 1. The data show that there are over 30 words in the Korean language to describe the varieties of pain experience even within this small sample. 2, There was low significant relationship between present pain intensity using visual analogue scale and the selected numbers of pain words from the pain questionnaire (p<.01). 3. In order to separate basic factors, a principal component analysis with varimax rotation was performed. The principal component analysis produced 8 factors. The proportion of variance explained by these factors was $71.0\%$. The first factor accounting $26.8\%$ of the variance was labeled 'cruelty and fear related pain' ; second 'pain produced from deep tissue' : third 'skin-punctuating related pain' ; and fourth 'miscellaneous and complicated pain'. Results of this study might be utilitzed in developing a pain questionnaire for pain patients.

• 여성건강간호학회지
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• 제14권1호
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• pp.56-65
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• 2008

Purpose: The study was to develop an instrument to measure grandmothering stress for Korean day-care grandmothers and to test the validity and reliability of the instrument Method: The items of the instrument were based on a literature review and secondary data, After content validity tests and a pilot test, 20 items were developed. In order to test the reliability and validity of the scale, data were collected from 126 grandmothers. Result: After a factor analysis, five factors and 15 items were selected. These explained 67.2% of the total variance. The first domain was termed 'Health problems', and explained 18.1 % of the total variance, and the second domain of 'Possibility of role substitution' explained 13.8%. The third and the fourth domains were 'Relations with adult children' and 'Grandchildren's characteristics' explaining 12.5% and 12.1 %, respectively. The last domain explained 10.8% of the total variance with the theme 'Restriction of social life' After accomplishing the reliability analysis, Cronbach's alpha coefficient was determined to be .75. Conclusions : This initial step in the development of a Grandmothering Stress Index is valuable to reflect the subject of Grandmothering stress in senior citizens in Korea. Future study should to refine the constitution of the instrument.

• 패션비즈니스
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• 제22권3호
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• pp.39-56
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• 2018

Since trusting in other consumers may refer to having similar needs and preferences on fashion goods with them, interpersonal trust can be a reliable and practical criterion for market segmentation online. Therefore, this study aims to identify fashion online consumers based on interpersonal trust. This study segments fashion consumers based on interpersonal trust and experience and describes characteristics of each segment by examining demo-psychographic and behavioral variables as well as clothing consumption values. An online survey was conducted to collect data from 426 adult consumers who had bought fashion goods from their patronized e-tailer in the past one month. They completed a self-administered questionnaire inquiring about interpersonal trust, trust in e-tailers, purchase intentions, clothing consumption values, and their purchasing behavior online. Two-step cluster analysis generated four segments: distrustful doers, trusting doers, inactive trusters, and distrusters. They exhibited different characteristics in gender, online experiences, interpersonal trust, clothing consumption values, trust in the e-tailers, and attitude toward the e-tailers. Providing a new effective segmentation base, this study suggests that fashion marketers identify customers with a high level of trust in other customers and develop an encouraging environment that customers can interact with others in order to increase the effectiveness of trust. Because customers with a higher level of interpersonal trust are likely to have stronger trust in e-tailers with, more favorable attitude and purchase intention, and highly perceive the value of clothing consumption than those who have a lower level of interpersonal trust.

• 보건교육건강증진학회지
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• 제32권5호
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• pp.73-81
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• 2015

Objectives: We tried to confirm physical activity of 1,000 Kcal per week was a meaningful point in controlling coronary artery disease risks in female older adults. Methods: Participants were 66 female older adults recruited from senior welfare center. Participants were provided with accelerometer (e-step, Kenz, Japan) for measuring daily energy expenditure. Graded exercise test was done for measuring aerobic fitness. Blood glucose and lipid were analyzed. Framingham risk score was calculated based on blood glucose, blood lipid, and smoking. These variables were compared between the group expended more than 1,000 Kcal/week and the group with energy expenditure below 1,000 Kcal/week. Results: The group expended over 1,000kcal/week showed to be superior to the counterpart group in following variables; AC(Abdominal Circumference), %BF, $HR_{rest}$(resting heart rate), $VO_{2peak}$, FBG, LDL-C, TG, BDI-II, QOL, AR(Absolute Risk), RR(Relative Risk). Conclusions: The group expended over 1,000 Kcal/week was likely to have less probability in CAD than group expended less than 1,000 Kcal/week. The result of this study suggests the important role of active daily life that can be replaced with that of regular exercise especially for those who are not available to do structured exercise.

• 대한수의학회지
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• 제61권3호
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• pp.25.1-25.7
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• 2021

The axolotl has extraordinary regeneration capacity compared to other vertebrates. This remarkable potential has been attributed to its life-long neoteny, characterized by the exhibition of embryonic characteristics at the adult stage. A recent study provided a detailed morphological analysis of the sperm morphology of the Ambystoma mexicanum using routine and detailed histological techniques. The primary purpose of the present study is to describe a simple and inexpensive method for evaluating the morphology of axolotl sperm. In this study, spermatophore structures were collected and spread on slides and air-dried. The slides were stained with periodic acid Schiff, toluidine blue, Masson's trichrome, Giemsa, Spermac, and Diff-Quik dye for a morphological examination. The slides were coated with gold/palladium for a scanning electron microscopy examination. The sperm of the axolotl consisted of an elongated head, a neck, and a flagellum covered with an undulating membrane. The lengths of the midpiece, tail, and head were 8.575 ㎛, 356.544 ㎛, and 103.661 ㎛, respectively. In the flagellum part, the wavy membrane structure, whose function has not been explained, surrounds the tail. The data obtained from this study will constitute an important step in designing future research on the reproductive and regeneration capacity of the axolotl.

• Psychiatry investigation
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• 제15권12호
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• pp.1130-1134
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• 2018

Objective Trichotillomania is a relatively common illness whose neurobiology is poorly understood. One treatment for adult trichotillomania, n-acetyl cysteine (NAC), has antioxidative properties, as well as effects on central glutamatergic transmission. Preclinical models suggest that excessive oxidative stress may be involved in its pathophysiology. Methods Adults with trichotillomania provided a blood sample for analysis of compounds that may be influenced by oxidative stress [glutathione, angiotensin II, ferritin, iron, glucose, insulin and insulin growth factor 1 (IGF1), and hepcidin]. Participants were examined on symptom severity, disability, and impulsivity. The number of participants with out-of-reference range oxidative stress measures were compared against the null distribution. Correlations between oxidative stress markers and clinical measures were examined. Results Of 14 participants (mean age 31.2 years; 92.9% female), 35.7% (n=5) had total glutathione levels below the reference range (p=0.041). Other oxidative stress measures did not have significant proportions outside the reference ranges. Lower levels of glutathione correlated significantly with higher motor impulsiveness (Barratt Impulsiveness Scale sub-score) (r=0.97, p=0.001). Conclusion A third of patients with trichotillomania had low levels of glutathione, and lower levels of glutathione correlated significantly with higher motor impulsiveness. Because NAC is a precursor for cysteine, and cysteine is a rate limiting step for glutathione production, these results may shed light on the mechanisms through which NAC can have beneficial effects for impulsive symptoms. Confirmation of these results requires a suitable larger follow-up study, including an internal normative control group.

• 비만대사연구학술지
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• 제1권2호
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• pp.66-73
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• 2022

Obesity is an increasing public health and medical issue worldwide. It has been associated with several comorbidities, including diabetes, cardiovascular disease, stroke, and cancer. Chronic kidney disease (CKD) is another important comorbidity of obesity. Other major causes of CKD include hypertension and diabetes. However, the association between obesity and CKD is often overlooked. Among patients with CKD, patients with obesity were more vulnerable to have rapid kidney function decline than that of those with normal weight. Additionally, CKD is more prevalent among patients with obesity. These aggravations are induced through multiple mechanisms, specifically metabolic impairment of obesity and mechanical burden because of increasing intraabdominal renal pressure. Furthermore, the inflammation and lipotoxicity, caused by obesity, are critical in the CKD aggravation in patients with obesity. To prevent this, all adult patients with obesity are tested for CKD. The workup includes the estimated glomerular filtration rate and regular follow-up. Step-wise management is required for patients with obesity with CKD. Prompt reduction and management of obesity effectively delay CKD progression among patients with obesity and CKD. Therefore, weight loss is a core management for patients with obesity and CKD. Based on several studies, this article focused on the association between CKD and obesity, as well as the diagnosis and weight management of patients with obesity and CKD.

• 한국운동역학회지
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• 제32권2호
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• pp.37-42
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• 2022

Objective: The aim of this study is to analyze the effect of the strength of the ankle support on the walking characteristics and ankle joints when men with flexible flat feet walk. Method: 13 adult male subjects (age: 23.9 ± 2.4 yrs, height: 173.0 ± 5.0 cm, weight: 76.9 ± 13.2 kg, Navicular Drop Test (NDT): 10.2 ± 0.8 mm) participated in this study. Each participant had to walk with the 3 conditions, barefoot, soft arch support and hard arch support, along a walkway while their kinematics was recorded at 100 Hz. Results: Based on the results of this study, it is considered that men with flexible flat feet should use hard arch support rather than bare feet to induce normal arch shape, relieve foot damage caused by excessive ankle joint abnormalities and improve stability. Conclusion: Our results for men with flat flexibility, there was a significant difference in the value of step length when walking was performed using two arch supports with different strengths. The angle of ankle dorsiflexion was significantly increased, and the ankle eversion angle was significantly decreased.