• 한국응용곤충학회지
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• 제23권2호
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• pp.132-136
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• 1984

전남지방의 밤 집단재배단지에서 피해가 확대되고 있는 밤바구미의 피해율, 발생시기, 토중의 월동부위, 월동후의 사충율의 변이에 대한 시험을 계획한 바 다음과 같은 결과를 얻었다. 1. 전남지방 밤 단지의 밤바구미 피해율은 담장 $26.4\%$, 광양 $21.1\%$, 승주 $12.9\%$였으며 이 지역은 참나무류가 많이 혼재되어 있었다. 2. 월동유충의 약 $3.33\%$가 1년차에 우화되었고, $22.78\%$가 2년차에, 그리고 $1.67\%$가 3년차로 계속 월동중이었다. 3. 밤바구미의 우화시기는 7월 중순부터 9월 중순까지였고 그 최성기는 8월 중순에서 9월 상순이었다. 4. 밤바구미 피해종실로부터의 유충 탈출시기는 10월 상순부터 11월 상순이었다. 5. 밤바구미의 난기는 5.95일, 용기는 17.17일, 성충의 수명은 암컷 9.10일, 숫컷 7.03일 이었으며 유충기간은 매우 다양하여 측정하기 어려웠다. 6. 밤바구미는 월동유충은 상중 $16\~26cm$에 많이 분포하였다. 7. 월동후 일수경과에 따른 사충율과는 고도의 유의상관관계$(r=0.970^{**})$가 인정되었다.

• 농업과학연구
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• 제22권1호
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• pp.49-53
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• 1995

차와 딸기등 많은 밭작물에 피해(被害)가 큰 간자와응애에 대(對)한 형태(形態)와 온도별(溫度別) 발육기간(發育期間) 및 성충수명(成蟲壽命)등을 조사한 결과는 다음과 같다. 1. 간자와응애의 크기에 있어서 자성충(雌成蟲) 체장(體長) 및 체폭(體幅)은 0.40mm와 0.22mm이었고 웅성충(雄成蟲)은 0.31mm와 019mm로서 자성충(雌成蟲)이 현저히 컸다. 2. 온도별(溫度別) 발육기간조사(發育期間調査)에서 미숙기를 살펴보면 $16^{\circ}C$가 24.8일(日)로 가장 길었으며 $32^{\circ}C$가 6.7일(日)로 가장 짧은 것을 알 수 있었다. 3. 성충수명(成蟲壽命)은 $16^{\circ}C$, $20^{\circ}C$, $24^{\circ}C$, $28^{\circ}C$, $32^{\circ}C$에서 각각 31.5일(日), 13.4일(日), 8.4일(日), 7.8일(日), 5.8일(日)로 온도가 낮을수록 수명이 길었다.

• Clinical and Experimental Reproductive Medicine
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• 제30권4호
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• pp.299-307
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• 2003

Objectives: The present study was conducted to evaluate the mobile transposon-like element, clone MTi7 (MTi7) expression in the mouse ovary and to determine its role(s) in the mouse oocytes by RNA interference (RNAi). Methods: MTi7 mRNA expression was localized by in situ hybridization in day5 and adult ovaries. Double stranded RNA (dsRNA) was prepared for c-mos, a gene with known function as control, and the MTi7. Each dsRNA was microinjected into the germinal vesicle (GV) stage oocytes then oocyte maturation and intracellular changes were evaluated. Results: In situ hybridization analysis revealed that MTi7 mRNA localized to the oocyte cytoplasm from primordial to preovulatory follicles. After dsRNA injection, we found 43-54% GV arrest of microinjected GV oocytes with 68%-90% decrease in targeted c-mos or MTi7 mRNA. Conclusions: This is the first report of the oocyte-specific expression of the MTi7 mRNA. From results of RNAi for MTi7, we concluded that the MTi7 is involved in the germinal vesicle breakdown in GV oocytes, and MTi7 may be implicated with c-mos for its function. We report here that RNAi provides an outstanding approach to study the function of a gene with unknown functions.

• 한국발생생물학회지:발생과생식
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• 제21권4호
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• pp.425-434
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• 2017

Polyploidy is occurred by the process of endomitosis or cell fusion and usually represent terminally differentiated stage. Their effects on the developmental process were mainly investigated in the amphibian and fishes, and only observed in some rodents as mammalian model. Recently, we have established tetraploidy somatic cell nuclear transfer-derived human embryonic stem cells (SCNT-hESCs) and examined whether it could be available as a research model for the polyploidy cells existed in the human tissues. Two tetraploid hESC lines were artificially acquired by reintroduction of remained 1st polar body during the establishment of SCNT-hESC using MII oocytes obtained from female donors and dermal fibroblasts (DFB) from a 35-year-old adult male. These tetraploid SCNT-hESC lines (CHA-NT1 and CHA-NT3) were identified by the cytogenetic genotyping (91, XXXY,-6, t[2:6] / 92,XXXY,-12,+20) and have shown of indefinite proliferation, but slow speed when compared to euploid SCNT-hESCs. Using the eight Short Tendem Repeat (STR) markers, it was confirmed that both CHA-NT1 and CHA-NT3 lines contain both nuclear and oocyte donor genotypes. These hESCs expressed pluripotency markers and their embryoid bodies (EB) also expressed markers of the three embryonic germ layers and formed teratoma after transplantation into immune deficient mice. This study showed that tetraploidy does not affect the activities of proliferation and differentiation in SCNT-hESC. Therefore, tetraploid hESC lines established after SCNT procedure could be differentiated into various types of cells and could be an useful model for the study of the polyploidy cells in the tissues.

• Journal of Chest Surgery
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• 제3권2호
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• pp.91-106
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• 1970

Two groups of left pulmonary-subclavian artery anastomosis were done in 26 adult mongorel dogs. For the first group. the distal end of the subclnvian artery was nnastomosed to the side of the left pulmonary artery, and for the second group, the subclavian end wns anastomosed to the proximal end of the left lower lobe pulmonary artery. Among them, 6 died of bleeding or anesthetic failure during the day of operation, 10 survived 1 to 106 days and the other 10 were sacrificed at various interval. To investigate the relationship between hemodynamic stress and temporal evolution of the pulmonary vascular lesions. the pulmona try and femoral artery pressures, arteriogram and pathohistological specimens were obtained. The following results were obtained. 1. The postoperative pulmonary artery pressures were within normal limits except 2 dogs in the first group, but in nil 12 dogs of the second group, they were in moderate to maked pulmonary hypertension level. 2 After subclavian pulmonary anastomosis, both groups dogs showed increased femond artery pulse pressure. 3. The pulmonary vascular changes were more severe and appeared earlier in the second group dogs compared with the first group.4. The earliest vascular changes appeared in the media of the small muscular arteries and arterioles. 5. Various vascular chaDges were produced in 2 or 3 months. Thereafter, the changes were stationary. 6. Among the first group, two long-term servivors (No. 705 & 713) which had normal pulmonary artery pressure under the anesthesia, also showed various vascular changes as other pulmonary hypertensive dogs. 7. In the early stage medial hypertrophy. interruptio~l of elastic lamellae were found in the small muscular arteries and arterioles, which were followed by intimal proliferation and thrombosis. These findings may suggest some evidences of trauma to the vessels. 8. Pulmonary arteriograms showed irregularity of the intima of the large and medium sized arteries, abrupt ending of some of the small arteries and narrowing of the anastomosis.

• 한국잡초학회지
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• 제31권1호
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• pp.103-111
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• 2011

물달개비는 한국의 논에서 문제되는 난방제 잡초중의 하나이다. 물달개비의 생물학적 방제 인자로 물달개비바구미를 선발하였다. 생물학적 방제에 이용하기 위해 2006년과 2007년 계대사육을 실시하여 형태적, 생태적 특징을 조사하였다. 이 종은 1년에 한세대만 경과하며 성충으로 월동하고, 성충은 6월 하순부터 9월 하순까지 발생한다. 물달개비바구미는 알에서 성충까지 $22{\pm}0.7$일이 소요된다. 주요 농작물과 수생식물 60종을 대상으로 기주특이성을 조사 결과 물달개비의 생물학적 방제 곤충으로서의 가능성을 보였다.

• Parasites, Hosts and Diseases
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• 제31권4호
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• pp.353-362
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• 1993

Fibricola seoulensis의 성체와 피낭유충에서 acid(Acpase)와 alkaline phosphatase (AIPase)의 분포와 동위효소유형 (유형)의 변화를 추구하고자 효소조직화학적 방법과 전기영동법을 이용하여 성체에서 Acpase는 pH 5가 최적의 활성이 나타났고. 분자량이 95 kDa. 85 kDa. 73 kDa. 62 kDa인 4종류의 동위효소가 동정되었다. 피낭유충에서 A쳬ase는 활성이 약하거나 나타나지 않았고. 분자량이 62 kDa인 1종류의 동위효소가 동정되었다 성체와 피낭유충에 AIPase는 pH 8에서 최적의 활성이 나타났고, 피낭유충의 생식원기에서 강한 활성이 나타났다.

• Parasites, Hosts and Diseases
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• 제45권2호
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• pp.121-128
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• 2007

Arthrostoma miyazakiense (Nematoda: Ancylostomatidae) is a hookworm species reported from the small intestines of raccoon dogs (Nyctereutes procyonoides) in Japan. Five Korean raccoon dogs (N. procyonoides koreensis) caught from 2002 to 2005 in Jeollanam-do (Province), a southeastern area of South Korea, contained helminth eggs belonging to 4 genera (roundworm, hookworm, whipworm, and Capillaria spp.) and cysts of Giardia sp. in their feces. Necropsy findings of 1 raccoon dog revealed a large number of adult hookworms in the duodenum. These hookworms were identified as Arthrostoma miyazakiense based on the 10 articulated plates observed in the buccal capsule and the presence of right-sided prevulval papillae. Eggs of A. miyazakiense were $60-65{\times}35-40{\mu}m$ (av, $62.5{\times}35{\mu}m$), and were morphologically indistinguishable from those of Ancyiostoma caninum. The eggs were cultured to infective 2nd stage larvae via charcoal culture, and 100 infective larvae were used to experimentally infect each of 3 mixed-bred puppies. All puppies harbored hookworm eggs in their feces on the 12th day after infection. This is the first report thus far concerning A. miyazakiense infections in raccoon dogs in Korea, and the first such report outside of Japan.

• Molecules and Cells
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• 제37권1호
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• pp.51-57
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• 2014

NOG1 is a nucleolar GTPase that is critical for 60S ribosome biogenesis. Recently, NOG1 was identified as one of the downstream regulators of target of rapamycin (TOR) in yeast. It is reported that TOR is involved in regulating lifespan and fat storage in Caenorhabditis elegans. Here, we show that the nog1 ortholog (T07A9.9: nog-1) in C. elegans regulates growth, development, lifespan, and fat metabolism. A green fluorescence protein (GFP) promoter assay revealed ubiquitous expression of C. elegans nog-1 from the early embryonic to the adult stage. Furthermore, the GFP-tagged NOG-1 protein is localized to the nucleus, whereas the aberrant NOG-1 protein is concentrated in the nucleolus. Functional studies of NOG-1 in C. elegans further revealed that nog-1 knockdown resulted in smaller broodsize, slower growth, increased life span, and more fat storage. Moreover, nog-1 over-expression resulted in decreased life span. Taken together, our data suggest that nog-1 in C. elegans may be an important player in regulating life span and fat storage via the insulin/IGF pathway.

• 전자공학회논문지
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• 제52권11호
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• pp.47-54
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• 2015

인터넷의 발달과 스마트폰 등과 같은 휴대기기 보급의 확산으로 트위터, 페이스북과 같은 SNS 사용자의 수가 증가하고 있다. 그리고 이와 함께 상품 광고, 비방 및 성인 콘텐츠 등을 게재함으로써 SNS를 오염시키는 콘텐츠 오염 문제 또한 점차 커지고 있다. 따라서 본 논문은 SNS에서의 콘텐츠 오염자를 탐지하기 위한 개선된 콘텐츠 오염자의 특징 추출 방법을 제안한다. 특히, 본 논문은 콘텐츠 오염자의 예측 및 분류 단계에서 새로운 사용자 데이터의 특징 값을 효율적으로 추출하기 위하여 전체 데이터를 대상으로 하는 일괄 처리 방식이 아니라 데이터 증가분만을 고려하는 점진적 접근 방법에 기초한 콘텐츠 오염자 특징 추출 방법을 제안한다. 그리고 제안한 방법이 일괄 처리한 방법과 비교해서 분류 정확도는 유지되고 시간 효율성이 향상되는 것을 실험을 통해 비교 평가한다.