• 약학회지
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• 제54권4호
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• pp.240-243
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• 2010

High-performance liquid chromatography (HPLC) screening method revealed that a propolis product marketed as a functional food contained an undeclared substance similar to tadalafil, the active ingredient of the prescription drug Cialis$^{(R)}$ approved for the treatment of male erectile dysfunction. In order to identify the illegal additive, the propolis product was extracted with methylene chloride, and the extract was purified further using semipreparative HPLC. The chemical structure of the isolated substance was elucidated based on IR, LC/MS-ESI, and $^1H$- and $^{13}C$-NMR spectroscopy, which showed the characteristics similar to tadalafil. The only difference was the substitution of the methyl group at the piperazinedione ring of tadalafil to the amino group of the identified illegal additive.

• 환경위생공학
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• 제15권3호
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• pp.70-74
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• 2000

The technology of malodorous substances treatment was classified physical separation and chemical destruction. This study was carried out to investigate the characteristics of malodorous substances treatment with the change of operating conditions from the ozone generator. The major results of this study were as follows : Removal efficiency by additive ozone rate was measured $NH_3$:95%(ozone additive rate : 2), $H_2S$ : 97%(ozone additive rate:4), $CH_3SH$ : 96%(ozone additive rate : 3), $(CH_3)_2S_2$ : 97%(ozone additive rate:5), $(CH_3)_3N$ : 95%(ozone additive rate : 1), $CH_3CHO$ : 95%(ozone additive rate : 2), $C_6H_5CHCH_2$ : 95%(ozone additive rate : 2).

• 대한화학회지
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• 제54권4호
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• pp.443-446
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• 2010

기능성식품으로 시판되는 건강음료를 HPLC 분석한 결과 발기부전치료제로 허가받은 비아그라$^{(R)}$의 활성주성분인 실데나필과 구조적으로 유사한 부정첨가물질이 발견되었다. 이 부정첨가물질의 구조를 규명하기 위해 건강음료를 methylene chloride로 추출한 다음 그 추출물을 semipreparative HPLC로 정제하였다. 분리된 물질의 화학구조는 IR, LC/MS-ESI, NMR 분광학으로 규명하였는데 실데나필과 유사한 특징을 보였고 구조상의 유일한 차이는 실데나필의 methylpiperazine 부분구조를 부정첨가물질에서 hydroxyethylpiperazine으로 치환한 것이었다.

• 한국세라믹학회지
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• 제25권5호
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• pp.561-569
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• 1988

Mixtures of very small amounts of additive, carbon and silica(about 0.46${\mu}{\textrm}{m}$) which synthesized by the hydrolysis of ethyl silicate, the molar ratio of SiO2/C was fixed to 1/10, was nitrided at 145$0^{\circ}C$. It was considered that the optimum amount of additive to promote the nitridation reaction was below 2.0wt%. By the addition of additive, the nitridation reaction was promoted and formation of $\beta$-Si3N4 was promoted at 145$0^{\circ}C$ for 1hour, but, the nitridation reaction was decreased and the ratio of $\alpha$/$\beta$ of Si3N4, was increased at 145$0^{\circ}C$ for 5 hours. The crystal phase was $\alpha$ phase and the nitridation reaction was promoted and the particle size of silicon nitride was become smaller by the addition of $\alpha$-Si3N4, but silicon nitride of whisker-like form was produced by the addition of transition elements. There was a difference in the lattice constants of $\alpha$-Si3N4, but no difference in its of $\beta$-Si3N4 according to kinds of added substance and reaction time.

• 한국표면공학회지
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• 제17권4호
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• pp.106-119
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• 1984

Brass was electrodeposited over the range of the current densities from 2 to 8 A/$dm^2$ in cyanide bath at 20 and 40$^{\circ}C$. The cathode overpotential increased and the cathode efficiency was decreased respectively with decreasing temperature, increasing current density and addition of organic substance. The perferred orientation of the deposits were associated with the cathode overpotential and the nucleation energy of lattice planes. The (111) preferred orientation developed at the low current density and low cathode overpotential (440-520mV). On the other hand, the (111)+(100) preferred orientation developed at higher cathode overpotential (528-680mV). The (111)+(100) preferred orientation developed over the whole range of overpotential in the cyanide solution with organic additive. The copper content of deposit decreased with increasing current density and decreasing temperature. The morphology of the deposits with no additive was the polygonal body type of structure and the structure of the cross section was columnar structure. The morphology of the deposits with additive, on the other hand, was fine crystallite type of structure. And the structure of the cross section of them was the finer granular structure.

• KSBB Journal
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• 제23권1호
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• pp.65-69
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• 2008

가축 질병의 제어를 위해 면역증강제인 베타글루칸 생성균으로 분리한 Paenibacillus polymyxa JB115의 배양액이 가축병원성 세균에 대한 항균성을 보임에 따라, 배양액의 동결건조 분말을 대상으로 항균물질의 특성을 조사하여 다기능 가축 사료첨가제로의 개발 가능성을 타진하였다. 그 결과, P. polymyxa JB115에서 생산된 항균물질은 E. coli, S. aureus, S. choleraesuis 및 P. aeruginosa 등 가축 병원성 세균들에 대해 살균 또는 정균 활성을 보였으며, 항균범위도 광범위하게 나타났다. 또한 이 물질은 높은 내열성과 pH 안정성을 지녀 배양액의 제형화에도 적합하였으며, 인공 담즙산 및 인공 위액에서도 안정하여 사료첨가제로 투여시 가축의 장내 병원세균에 대해서 항균 활성을 충분히 나타낼 수 있을 것으로 판단되었다. 상기의 결과로부터 P. polymyxa JB115의 배양액은 베타글루칸 이외에도 가축 병원성 세균에 대한 항균물질을 지니고 있어서, 다기능 사료첨가제로 개발 가능함을 확인하였다.

• International journal of advanced smart convergence
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• 제13권1호
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• pp.194-200
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• 2024

In the past, antibiotics and antimicrobial substances have been used for the purpose of promoting the growth of livestock or treating livestock, but various problems such as the presence of livestock products or resistant bacteria have emerged. Recently, regulations on the use of antibiotics have been strengthened worldwide, and probiotics are attracting attention as an alternative. Probiotic microorganisms have already been used for human use, such as intestinal abnormal fermentation, diarrhea, and indigestion. In livestock, beneficial microorganisms are increasing in use for the purpose of improving productivity, such as promoting livestock development and preventing diarrhea. Therefore, it is advisable to understand livestock probiotics in deeper and think about effective uses. The role of probiotics in the livestock sector is made with microorganisms themselves, so it is a substance that promotes livestock growth and improves feed efficiency by settling in the intestines of livestock, suppressing the growth of other harmful microorganisms, helping digestion and absorption of ingested feed, and helping to synthesize other nutrients. There is a need for a probiotic that suppresses intestinal bacteria by supplying probiotics used as a means to minimize the effects of stress in livestock management, thereby suppressing disease outbreaks by maintaining beneficial microorganisms and suppressing pathogenic microorganisms. The purpose of this paper is to develop a brand of feed additive probiotics to improve health conditions due to increased feed intake, improve the efficiency of use of feed nutrients, inhibit the decomposition and production of toxic substances, increase immunity, reduce odor in livestock, and improve the environment. We investigated and analyzed feed additive probiotics already on the market, and developed the naming and logo of suitable feed additive probiotic brands in livestock. We hoped that the newly developed product will be used in the field and help solve problems in the livestock field.

• E2M - 전기 전자와 첨단 소재
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• 제10권7호
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• pp.719-726
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• 1997

This research was conducted to analyze the change of surface tension, viscosity, streaming current and conductivity of transformer oil when it were injected with the interface active substances.(anionic:S-111, cationic:S-121, amphoteric:S-131) The changes properties of the surface tension and viscosity of the oil which were injected with the interface active substances were divided into the changes area and the minimum reduction area. The surface tension and viscosity of the oil which were injected with three different kinds of interface active substances showed remarkable change at the point where the concentration of the substance in anionic, in cationic and in amphoteric were 100[ppm], 10[ppm] and 1[ppm] respectively. The streaming current and conductivity of the same sample oil were also changed at the same densities of the surface tension and viscosity. For this factor, it was possibile for us to interpret the mechanism of the streaming current and conductivity. Therefore the interface active substances of the three kinds were injected into the oil within the limit of optimal volume, prevention effects of electrification were showed more excellence than unmixed insulating oil.

• 한국식품영양학회지
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• 제24권1호
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• pp.94-100
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• 2011

This study identified the formula of the antioxidant substance separated from the ethyl acetate and the butanol extract and tested the antioxidant properties with the electron donating ability(EDA). Each phase with the fractionated methanol extract from mulberry leaf was screened in advance for the antioxidant substance with EDA. As the result, activity appeared in the ethyl acetate and butanol phase and the antioxidant component was separated. As the consequence, 2 components from the ethyl acetate phase and 1 from the butanol phase were separated, among which the structures of the components from ethyl acetate were determined by wogonin and linarin, whereas the structure of the component from the butanol phase was determined by pectolinarin. In the screening of antioxidant activity by the scavenging effect of the DPPH radical, the wogonin and linarin components from ethyl acetate phase showed more powerful antioxidant property than the component from butanol. The results from this study indicate that the chemical compound separated from the ethyl acetate extract has more powerful antioxidant property than the one separated from the butanol extract. The components separated from the ethyl acetate extract were wogonin and linarin, which are flavonoids, whereas the component from butanol was pectolinarin. Therefore, this study suggested that the feasibility of mulberry leaf as a functional food additive and its value as a natural antioxidant is very high.

• Journal of Dairy Science and Biotechnology
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• 제34권2호
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• pp.137-144
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• 2016

본 시험은 GMP 내 기본적으로 7%로 결합되어 있는 sialic acid의 함유량을 그대로 보유하도록 제조한 유청단백가수분말(시험물질명: 7%-GNANA)을 기능성 식품 원료 개발함에 최종 연구목표를 두었다. 시험물질은 GMP(7% sialic acid 함유)를 원료로 하고, 여기에 식품첨가물로 허용된 효소인 Alcalase를 사용하여 지표성분인 sialic acid를 100% 효율로 분리시킨 후, 동결 건조한 7%-GNANA(7% sialic acid와 GMP 단백질로 구성, 제품명: HELICOBACTROL-7)을 (주)한일바이오메드사(한국)에서 공여 받아 GLP 가이드라인에 따라 미생물복귀돌연변이시험을 실시하였다. 미생물에 대한 돌연변이 유발성 유무를 검색하기 위해 히스티딘 요구성 균주인 Sal. typhimurium TA98, TA100, TA1535 및 TA1537과 트립토판 요구성 균주인 E. coli WP2uvrA를 이용하였다. 미생물복귀돌연변이시험은 시험물질을 5단계의 농도군(0, 61.7, 185, 556, 1,670, $5,000{\mu}g/plate$)으로 하여 평가하였다. 본 시험을 통한 평가결과, 대사활성계 존재 유무와 관계없이 모든 균주에서 시험물질의 각 농도에 의한 복귀돌연변이 유발원 양성기준인 콜로니 생성수치가 재현성 있는 증가를 나타내지 않았으며, 용량의존성도 확인되지 않았다. 결론적으로, 시험물질인 7% G-NANA의 식품첨가물로서 등록을 위하여 수행한 미생물돌연변이시험에서 안전성이 확인되었다.