• 한국수정란이식학회지
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• 제30권4호
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• pp.299-303
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• 2015

KO mice provide an excellent tool to determine roles of specific genes in biomedical filed. Traditionally, knockout mice were generated by homologous recombination in embryonic stem cells. Recently, engineered nucleases, such as zinc finger nuclease, transcription activator-like effector nuclease and clustered regularly interspaced short palindromic repeats (CRISPR), were used to produce knockout mice. This new technology is useful because of high efficiency and ability to generate biallelic mutation in founder mice. Until now, most of knockout mice produced using engineered nucleases were C57BL/6 strain. In the present study we used CRISPR-Cas9 system to generate knockout mice in FVB strain. We designed and synthesized single guide RNA (sgRNA) of CRISPR system for targeting gene, Abtb2. Mouse zygote were obtained from superovulated FVB female mice at 8-10 weeks of age. The sgRNA was injected into pronuclear of the mouse zygote with recombinant Cas9 protein. The microinjected zygotes were cultured for an additional day and only cleaved embryos were selected. The selected embryos were surgically transferred to oviduct of surrogate mother and offsprings were obtained. Genomic DNA were isolated from the offsprings and the target sequence was amplified using PCR. In T7E1 assay, 46.7% among the offsprings were founded as mutants. The PCR products were purified and sequences were analyzed. Most of the mutations were founded as deletion of few sequences at the target site, however, not identical among the each offspring. In conclusion, we found that CRISPR system is very efficient to generate knockout mice in FVB strain.

• Environmental Engineering Research
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• 제20권1호
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• pp.79-87
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• 2015

To treat leather industry wastewater (LIW) containing high nitrogen concentration, eight aerobic denitrifiers were isolated from sludge existing in an LIW-treatment aeration tank. Among them, one strain named as KH8 had showed the great ability in denitrification under an aerobic condition, and it was identified as Pseudomonas aeruginosa R12. The aerobic denitrification ability of the strain KH8 was almost comparable to its anaerobic denitrification ability. In lab-scale aerobic denitrifications performed in 1-L five-neck flasks for 48 hr, denitrification efficiency was found to be much improved as the strain KH8 held a great majority in the seeded cells. From the nitrogen balance at the cell-combination ratio of 10:1 (the strain KH8 to the other seven isolates) within the seeded cells, the percentage of nitrogen loss during the aerobic denitrification process was estimated to be 58.4, which was presumed to be converted to $N_2$ gas. When these seeded cells with lactose were applied to plant-scale aeration tank for 56 day to treat high-strength nitrogen in LIW, the removal efficiencies of $COD_{Cr}$ and TN were achieved to be 97.0% and 89.8%, respectively. Under this treatment, the final water quality of the effluent leaving the treatment plant was good enough to meet the water-quality standards. Consequently, the isolated aerobic denitrifiers could be suitable for the additional requirement of nitrogen removal in a limited aeration-tank capacity. To the best of our knowledge, this is the first report of aerobic denitrifiers applied to plant-scale LIW treatment.

• 한국지반공학회논문집
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• 제19권5호
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• pp.49-58
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• 2003

본 연구에서는 지중배전선로를 건설함에 있어 전력케이블용 보호관으로 사용되고 있는 PE파형관의 매설깊이별 작용압력-변형률 특성을 분석하기 위해 고전이론식, 전산해석, 실내 모형토조실험 및 현장실증실험을 수행하였으며, 이들 해석의 비교 분석을 통해 지중매설관의 거동에 영향을 미치는 주요인자를 밝혀내고, 더불어 현장시공시에 지중매설관의 안정성을 확보할 수 있는 매설깊이를 분석하였다. 각 해석방법에 적용된 매설형태는 A-1급 도로포장 아래 매설깊이 80cm, 100cm, 120cm지점에 PE파형관이 묻히고, 외부하중으로 DB-24차량의 후륜하중 9.6ton이 매설관 상단 중앙에 정차했을 때 관이 받는 작용압력-변형률 특성을 분석하였다. 여러 가지 해석결과, KS-C8455에 명시되어 있는 지중연성관의 허용변형률 기준인 3.5%에 만족하는 매설깊이는 약 80cm 이상인 것으로 판명되었다. 특히, 현장 실증 실험결과, 지중매설관의 거동에 영향을 주는 주요인자인 토압, 윤압 외에 다짐에너지가 관의 변형에 상당한 영향을 미치는 것으로 밝혀졌고, 관에 발생하는 변형량의 약 90% 이상이 다짐에너지라는 인자에 의해 발생하는 것으로 계측되었다.

• 열처리공학회지
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• 제32권3호
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• pp.107-112
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• 2019

In order to investigate the effect of plastic deformation and annealing process parameters on strength and electrical conductivity of Cu-Fe alloys, Cu-10wt%Fe, Cu-15wt%Fe alloys were drawn up to ${\eta}=4$ and annealed in the temperature range of $300^{\circ}C$ to $700^{\circ}C$, followed by measurements of tensile strength and electric conductivity. As draw strain increases, tensile strength increases while electrical conductivity decreases. These observations result from reduction of dislocation density and decrease in Fe fiber spacing. Raising annealing temperature brought about decrease of tensile strength and increase of electrical conductivity up to $500^{\circ}C$, being followed by decreasing above $500^{\circ}C$. Such results are thought to be caused by decrease of dislocation density below $500^{\circ}C$ and rapid solubility increase of Fe in Cu above $500^{\circ}C$. For the purpose of obtaining both high strength and high conductivity, annealing process should be incorporated just prior to reaching to final draw strain. For Cu-10wt%Fe alloy, the tensile strength 706.9 MPa and the electrical conductivity 54.34%IACS were obtained through the processes of drawing up to ${\eta}=3$, annealing at $500^{\circ}C$ for 1 hour and additional drawing up to total strain of ${\eta}=4$.

• 열처리공학회지
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• 제37권2호
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• pp.79-85
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• 2024

The effects of annealing heat treatment and the addition of Cu element on the shape memory effect of the NiTi-based alloy were investigated by analyzing differential scanning calorimeter results and characterizing recovery rate through 3D scanning after Vickers hardness test. Through 3D scanning of impressions after Vickers hardness test, the strain recovery rates for specimens without annealing treatment and annealed specimens at 400, 450, and 500℃ were measured as 45.96%, 46.76%, 52.37%, and 43.57%, respectively. This is because as the annealing temperature increases, both B19' and NiTi₂ phases, which can impede martensitic transformation, are incorporated within the NiTi matrix. Particularly, additional phase transformation from R-phase to B19' observed in specimens annealed at 400 and 450℃ significantly contributes to the improvement in strain recovery rates. Additionally, the results regarding the Cu element content indicate that when the total content of Ni and Cu is below 49.6 at.%, the precipitation of fine B19' and NiTi₂ phases within the matrix can greatly influence the transformation enthalpy and temperature range, resulting in relatively lower strain recovery rates in NiTi alloys with a small amount of Cu element produced in this study.

• Earthquakes and Structures
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• 제5권5호
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• pp.589-605
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• 2013

The effect of dead loads on dynamic responses of a uniform elastic beam subjected to moving loads is examined by means of a governing equation which takes into account initial bending stresses due to dead loads. First, the governing equation of beams which includes the effect of dead loads is briefly presented from the author's paper (1990, 1991, 2010). The effect of dead loads is considered by a strain energy produced by conservative initial stresses caused by the dead loads. Second, the effect of dead loads on dynamical responses produced by moving loads in simply supported beams is confirmed by the results of numerical computations using the Galerkin method and Wilson-${\theta}$ method. It is shown that the dynamical responses by moving loads are decreased remarkably on a heavyweight beam when the effect of dead loads is included. Third, an approximate solution of dynamic deflections including the effect of dead loads for a uniform beam subjected to moving loads is presented in a closed-form for the case without the additional mass due to moving loads. The proposed solution shows a good agreement with results of numerical computations with the Galerkin method and Wilson-${\theta}$ method. Finally it is clarified that the effect of dead loads on elastic uniform beams subjected to moving loads acts on the restraint of the transverse vibration for the both cases without and with the additional mass due to moving loads.

• Design & Manufacturing
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• 제12권1호
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• pp.18-25
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• 2018

Both injection and injection molding dies have evolved into advanced technology. Product quality is also evolving day after day. Therefore, the conditions of the injection mold and the injection conditions are becoming important. In order to improve the quality of the product, the Hardware part of the mold has developed as an advanced technology, and the Software part has also developed with advanced technology. This study deals with the cooling part, which is part of the hardware. In addition to fluid cooling, which is commonly used in the industry, by using gas cooling identify the phenomena that appear on the surface of the product and the critical point strain of the product to find the optimal cooling. Electronic parts and automobile parts whose surface condition is important, the cooling process is important to such a degree that they are divided with good products and defective products according to the cooling process at the time of injection. By controlling this important cooling and reducing the injection time with additional cooling, the product quality can be increased to the highest production efficiency. In addition, high efficiency can be achieved without additional investment costs. This study was conducted to apply these various advantages in the field.

• Structural Engineering and Mechanics
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• 제55권2호
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• pp.435-459
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• 2015

Parametric resonance of shear deformable composite skew plates subjected to non-uniform (parabolic) and linearly varying periodic edge loading is studied for different boundary conditions. The skew plate structural model is based on higher order shear deformation theory (HSDT), which accurately predicts the numerical results for thick skew plate. The total energy functional is derived for the skew plates from total potential energy and kinetic energy of the plate. The strain energy which is the part of total potential energy contains membrane energy, bending energy, additional bending energy due to additional change in curvature and shear energy due to shear deformation, respectively. The total energy functional is solved using Rayleigh-Ritz method in conjunction with boundary characteristics orthonormal polynomials (BCOPs) functions. The orthonormal polynomials are generated for unit square domain using Gram-Schmidt orthogonalization process. Bolotin method is followed to obtain the boundaries of parametric resonance region with higher order approximation. These boundaries are traced by the periodic solution of Mathieu-Hill equations with period T and 2T. Effect of various parameters like skew angle, span-to-thickness ratio, aspect ratio, boundary conditions, static load factor on parametric resonance of skew plate have been investigated. The investigation also includes influence of different types of linearly varying loading and parabolically varying bi-axial loading.

• ETRI Journal
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• 제38권4호
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• pp.787-797
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• 2016

Mining, construction, and other special vehicles for heavy use are designed to work under high-performance and off-road working conditions. The driving and executive mechanisms of the support structures and superstructures of these vehicles frequently operate under high loads. Such high loads place the equipment under constant risk of an accident and can jeopardize the dynamic stability of the machinery. An experimental investigation was conducted on a refuse collection vehicle. The aim of this research was to determine the working conditions of a real vehicle: the kinematics of the waste container, that is, a hydraulic rotate drum for waste collection; the dynamics of the load manipulator (superstructure); the vibrations of the vehicle mass; and the strain (stress) of the elements responsible for the supporting structure. For an examination of the force (weight) on the rear axle of a heavy vehicle, caused by its own weight and additional load, a universal measurement system is proposed. As a result of this investigation, we propose an alternative system for continuous vehicle weighing during waste collection while in motion, that is, an on-board weighing system, and provide suggestions for measuring equipment designs.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2003년도 추계학술대회논문집
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• pp.148-151
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• 2003

In order to achieve reliable but cost-effective crash simulations of stamped parts, sheet forming process effects were incorporated in simulations using the ideal forming theory mixed with the 3D hybrid membrane/shell method, while the subsequent crash simulations were carried out using a dynamic explicit finite element code. Example solutions performed for forming and crash simulations of I- and S-shaped rails verified that the proposed approach is cost-effective without sacrificing accuracy. The method required a significantly small amount of additional computation time, less than 3% for the specific examples, to incorporate sheet forming effects to crash simulations. As for the constitutive equation, the combined isotropic-kinematic hardening law and the non-quadratic anisotropic yield stress potential as well as its conjugate strain-rate potential were used to describe the anisotropy of AA6114-T4 aluminum alloy sheets.