• Journal of Plant Biotechnology
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• v.7 no.1
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• pp.51-55
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• 2005

Many cloning vectors in which cDNAs can be inserted to the sense orientation have been developed. Uni-ZAP XR vector (Stratagene) should contain clones that are oriented to sense direction with respect to T3 RNA polymerase primer. Unexpectedly, large portions of cDNAs in Chinese cabbage cDNA library showed unusual insertions, antisense orientation and a hybrid of two different clones. Using two clones, 4H03 and 53-B10, derived from different cDNA libraries, we proposed and demonstrated the possibility of unusual-construct formation by in vitro translation and northern blot analysis. The 4H03 clone was inserted with inverse direction, and its transcript and translation product could be produced by T7 RNA polymerase, indicating that this clone is definitely inserted into inverse orientation. The 53-B10 that contains two independent genes was turned out to be a hybrid in which two genes are inserted to opposite direction each other. All unusual constructs might be due to the presence of small fragments of DNA, like adapter. However, the mechanism underlined the formation of unusual constructs is still remain to be solved.

• Korean Journal Plant Pathology
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• v.10 no.3
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• pp.228-234
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• 1994

Genomic RNA was extracted from Cy strain of odontoglossum ringspot tobamovirus (ORSV-Cy) isolated from infected leaves of tobacco cv. Samsun. Size of the genomic RNA was about 6.6 kb in length. The genomic RNA was fractionated using Sephadex G-50 column chromatography into 2 fractions. They were polyadenylated at their 3'-end using E. coli poly(A) polymerase. Polyadenylated viral RNA was recovered by oligo (dT) primer adapter containing NotI restriction site and Moloney murine leukemia virus SuperScript reverse transcriptase (RNase H-). Second-strand cDNA was synthesized by using E. coli DNA ligase, E. coli DNA polymerase I and E. coli RNase H. Recombinant plasmids containing cDNAs for ORSV-Cy RNA ranged from about 800 bp to 3,000 bp. Among the selected 238 recombinants, pORCY-124 clone was the largest one covering 3'-terminal half of the viral RNA. This clone contained two restriction sites for EcoRI and XbaI and one site for AccI, AvaI, BglII, BstXI, HindIII, PstI, and TthIII 1. respectively. The clone contained partial viral replicase, a full-length movement protein and a complete coat protein genes followed by a 3' untranslated region of 414 nucleotides based on restriction mapping and nucleotide sequencing analyses. Clones pORCY-028, -068, -072, -187 and -224 were overlapped with the pORCY-124. Clones pORCY-014 and -095 covered 5' half upstream from the middle region of the viral RNA, which was estimated based on restriction mapping and partial sequence analysis. Constructed cDNA library covered more than 90% of the viral genome.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.41 no.8
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• pp.974-985
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• 2016

Riverbed Modeler, which is a commercial packet-level discrete event simulator is used to model, design, and simulate complicated communication protocols and large-scale network. Riverbed Modeler got credit for its reliability in field of network simulation. In the MANET simulation environment using Riverbed Modeler, it is very complicated to add a new routing protocol into existing architecture of routing protocols because it is required lots of modifications of protocol recognition. In this paper, we propose Routing Adding Framework which can reduce errors or mistakes during modifying the existing routing support architecture. Routing Adding Framework is provided as a adapter API for protocol recognition. and it is only minimum modifications for protocol identifiers when a new routing protocol is added to the child process of manet_mgr process which manages routing protocols for IP layer. With Routing Adding Framework, we can reduce less than half modification. Then, we shows an example of implementation of a hybrid routing protocol AntHocNet using Routing Adding Framework, and we verify its design and application of the Routing Adding Framework by obtaining simulation result with similar result given by AntHocNet.

• Journal of Korean Library and Information Science Society
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• v.49 no.4
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• pp.147-166
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• 2018

This study is to suggest the considerations in the rules for authorized access points for collocation of music work by figuring out the directions of authorized access points in FRBR, LRM, ICP 2016, RDA, and BIBFRAME, and by analyzing RDA rules for attributes and authorized access points of music works and expression and VIAF examples. First, an aggregated authorized access points were suggested as the direction of authorized access points, and original title may be selected as preferred title and the authorized access point may be based on forms in one of the languages suited to the users, if the original title is not normally suited. Second, music works's authorized access points is consisted of composer authorized access point and preferred title, and of adapter's authorized access point and preferred title in case of lacks of responsibility in composer. Also, the authorized access point of Korean traditional music work must be reviewed according to work types considering the responsibility of composer. Third, the controlled vocabularies for name of music type, medium of performance, and key could be considered for describing the attributes of work and expression. This study would be the foundation study for the authorized access point of music work, and additional research should be completed through surveying music user's need.

• Journal of KIISE:Computer Systems and Theory
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• v.32 no.11_12
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• pp.557-565
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• 2005

This paper presents the development and implementation of a zero-copy file transfer mechanism that improves the efficiency of file transfers for PC cluster systems using hardware-based VIA(Virtual Interface Architecture) network adapters. VIA is one of the representative user-level communication interfaces, but because there is no library for file transfer, one copy occurs between kernel buffer and user boilers. Our mechanism presents a file transfer primitive that does not require the file system to be modified and allows the NIC to transfer data from the kernel buffer to the remote node directly without copying. To do this, we have developed a hardware-based VIA network adapter, which supports the PCI 64bit/66MHz bus and Gigabit Ethernet, as a NIC, and implemented a zero-copy file transfer mechanism. The experimental results show that the overhead of data coy and context switching in the sender is greatly reduced and the CPU utilization of the sender is reduced to $30\%\~40\%$ of the VIA send/receive mechanism. We demonstrate the performance of the zero-copy file transfer mechanism experimentally. and compare the results with those from existing file transfer mechanisms.