• 한국입자에어로졸학회지
• /
• 제18권2호
• /
• pp.37-50
• /
• 2022

Due to the syndrome coronavirus 2 (SARS-CoV-2) pandemic, the subway passenger cabin should be continuously sterilized. However, a disinfectant such as chlorine is toxic and can lead to different issues to human health. In this paper, we introduced a novel disinfectant based on natural product (Dendropanax morbifera extract). Via ultra-high performance liquid chromatography - mass spectrometer (UHPLC-MS), different compounds from Dendropanax morbifera extract showed antivirus potentials. Antimicrobial experiments confirmed that the air-disinfectant containing Dendropanax morbifera can eliminate harmful microorganisms including Gram (-), Gram (+), and yeast within 5 mins. The as-prepared air-disinfectant also showed high antivirus activity against H1N1, HRV, and EV71. Deodorization test also indicates that the as-prepared air-disinfectant can lower the harmful gas such as ammonia and trimethylamine in the atmosphere. To evaluate the potential of air-disinfectant containing Dendropanax morbifera in practical applications, different safety tests including acute oral toxicity, acute skin irritation, and eye irritation were conducted. Results showed that the as-prepared disinfectant did not negatively affect tested animals during these safety investigations.

• 혜화의학회지
• /
• 제14권1호
• /
• pp.113-128
• /
• 2005

1. Acute laryngitis is a hoarse voice or the complete loss of the voice because of irritation to the vocal folds. 2. Acute laryngitis belongs with the GeupHuEum, HuBi, HuPung in oriental medicine. 3. GeupHuEum is caused by wind and cold, weak of lung and kidney, evil energy of liver, sore throat, etc. It is treated with the methods of cooling lung and wetting, removing heat and changing phlegm, etc. 4. Hubi is caused by fire and wind, dampness, large lung. It is treated with the methods of removing heat and antidote, reinforcing and descending fire, bleeding by acupuncture, vomiting. 5. Hupung is caused by phlegm and heat of lung and stomach, wind and heat. It is treated with the methods of dispersing wind and removing heat and changing phlegm by medicine, acupuncture, moxibustion, vomiting, fumigation.

• 한국환경보건학회지
• /
• 제5권1호
• /
• pp.1-9
• /
• 1978

Biological safety, physico-chemical and microbiological experiments were carried out in order to study the possibility of radiation sterilization on medical products, such as polyethylene and polystyrene bottles, gauze sponges and surgical silk suture. Results are as follows: 1. Biological test on plastic samples as to acute systemic toxicity, eye irritation, skin irritation, pyrogen, haemolysis showed satisfactory results. But physico-chemically, the oxidizable matter, nonvolatile residues, residue on ignition seemed to be increased slightly in irradiated samples, though the experimental results were within the range of U.S.P. 19, N.F. and K.P. III. 2. After irradiation, both plastic and gauze sponges showed considerable decrease in pH. 3. Most medical products were sterilized by 1.5 Mard. For the sterilization of the suigical silk suture, 4.5 Mrad is required dul to its radiation resistant micro-organisms. 4. Biologically, total dose of 2.5 Mrad was safe enough to sterilize the medical products, and physico-chemically the results are acceptable according to U.S.P. 19 and K.P. III. However, better results may be expected when the quality of material and additives are improved.

• 한국임상수의학회지
• /
• 제34권3호
• /
• pp.204-207
• /
• 2017

We report the successful treatment of feline acute bullous keratopathy (FABK) and corneal perforation with a conjunctival hood graft. Two cats with FABK and corneal perforation had severe corneal edema with large central bullae. Their corneas were thinned and perforated. Conjunctival hood grafts were performed. All corneas recovered after 3-4 weeks. The grafts were then removed from the cornea using superficial keratectomy and no ocular irritation was observed. One month later, the corneas had recovered, although there was very mild fibrosis. The cats had normal vision without any corneal problems at this time. Conjunctival hood graft was thus useful for the treatment of severe FABK and allowed the cats to maintain vision.

• 대한화장품학회지
• /
• 제25권4호
• /
• pp.145-154
• /
• 1999

A novel retinol derivative, polyethoxylated retinamide(Medimin A) was synthesized, as an anti-aging agent. Collagen synthesis, skin permeation, stability, and toxicity of Medimin A were evaluated and compared with those of retinol and retinyl palmitate. In vitro collagen synthesis was evaluated by quantitative assay of $[^3H]-proline$ incorporation into collagenase sensitive protein in fibroblast cultures. For in vitro skin permeation experiments, Franz diffusion cells(effective diffusion area: 1,766 $cm^2$) and the excised skin of female hairless mouse aged 8 weeks were used, The stabilities of retinoids were evaluated at two different temperature($25^{\circ}C\;and\;40^{\circ}C$) and under UV in solubilized state and in O/W emulsion. To estimate the safety, acute oral toxicity, acute dermal toxicity, primary skin irritation, acute eye irritation and human patch test were performed. The effect of Medimin A on collagen synthesis was similar to that of retinol. The skin permeability of Medimin A was higher than those of retinol and retinyl palmitate. The Medimin A was more stable than retinol and retinyl palmitate. Medimin A was nontoxic in various toxicological tests. These results suggest that Medimin A would be a good anti-aging agent for enhancing bioavailability and stability.

• 농약과학회지
• /
• 제17권4호
• /
• pp.478-481
• /
• 2013

본 연구는 병해충 관리용 유기농업자재 허용물질로 지정된 식물추출물 중 님 추출물(미얀마산, 인도산)과 고삼추출물(중국산)의 독성 평가를 위해 피부자극성과 안점막자극성시험을 수행하였다. 시험결과, 원산지가 다른 님 추출물 2종은 모두 피부자극성과 안점막자극성이 없는 것으로 평가되었고, 고삼 추출물은 피부자극성은 없었고 안점막자극성이 강도로 나타났으나 시간이 경과함에 따라 자극이 경감하는 것으로 평가됐다. 따라서 안점막자극성이 강한 것으로 평가된 고삼 추출물을 유기농업자재로 사용할 때 농작업자에 대한 안전대책이 필요한 것으로 판단된다.

• 분석과학
• /
• 제34권6호
• /
• pp.275-283
• /
• 2021

본 연구에서는 화학 물질의 급성 안자극 시험을 수행하기 위한 세포 기반 미세유체 칩의 개발과 응용에 관한 연구를 수행하였다. 포토리소그래피와 소프트리소그래피 공정을 이용하여 미세유체 칩을 제작하였으며, 칩은 배양 면적이 다른 3개의 세포 배양 구획으로 이루어져 있다. 세포 기반 안자극 시험은 토끼 각막 상피 세포를 사용하여 수행하였다. 미세유체 칩에 배양된 세포에 화학 물질 수용액을 처리한 후 일정한 간격으로 세포를 관찰하고, 생존율 곡선을 기반으로 세포 사멸에 대한 속도 상수를 계산하였다. 세포-세포 사이의 연접, 세포-기판 사이의 부착, 초기 세포 수 변화가 세포 사멸 속도에 미치는 영향을 조사하여 미세유체 칩의 성능을 검증하였다. 안자극 시험의 표준물질인 sodium dodecylsulfate (SDS) 수용액의 다양한 농도 조건에서 안자극 시험을 수행하였다. 화학 물질의 수용액에 300초 동안 노출시킨 세포의 생존율을 이용하여 안자극을 시험하였다. 최종적으로 미세유체 칩의 각 구획에 대한 가중치를 기반으로 독성 점수(toxicity score, TS) 산출식을 얻었다. 본 연구에서 개발한 세포 기반 미세유체 칩은 화장품과 제약에 사용되는 화학 물질의 안자극 시험에 활용될 수 있을 것이다.

• Biomolecules & Therapeutics
• /
• 제15권1호
• /
• pp.7-15
• /
• 2007

There have been various animal models of skin inflammation. These models have been used for establishing anti-inflammatory activity of the topical agents including cosmetics. Here, the molecular mechanisms of most widely-used animal models of skin inflammation including contact irritation, acute and chronic inflammation, and delayed-type hypersensitivity are summarized. Against these animal models, varieties of plant flavonoids showed anti-inflammatory activity. The action mechanisms of anti-inflammation by topical flavonoids are presented. A therapeutic potential of flavonoids is discussed.

• 한국산학기술학회:학술대회논문집
• /
• 한국산학기술학회 2010년도 춘계학술발표논문집 2부
• /
• pp.910-914
• /
• 2010

A collision between a barge and an oil tanker of Hebei Spirit caused the release of an estimated 12,547 tons of light crude oil into the Yellow Sea off the west coast of Taean-gun, Korea on December 7, 2007. This study was aimed to evaluate exposure conditions and acute health effects in soldiers participating in the Hebei Spirit oil spill cleanup activities and the association between these and the nature of the work and use of protection devices. The sample comprised 2,624 soldiers stratified by working area and number of working days. We divided working area into the coast of Taean-gun(highly polluted area) and other areas affected by Hebei Spirit oil spill. And we divided number of working days into 1-7 days, 8-14 days, 15-21 days and above 22 days. Data were obtained via a structured, self-administered questionnaire and included information on working area, type of working, number of working days, use of protective materials, and acute health effects. Acute health effects were classified into 5 groups: neurological, respiratory, dermatologic, ophthalmic and other symptoms. Data analysis was performed using unconditional logistic regression used to compute odds ratios and 95% confidence intervals. On logistic regression analysis, working on the coast of Taean-gun was significantly associated with increased risk of acute health problems: headache, dizziness, nausea, general fatigue, insomnia, flushed face, sore throat, dry throat, runny nose, cough, sputum, skin irritation, sore eyes, injection of conjunctiva, tear, and low back pain. Furthermore soldiers working more than 21 days were significantly associated with increased risk of acute health problems: insomnia, flushed face, sore throat, runny nose, cough, sputum, tear, low back pain and fever. Accordingly, the exposure to the oil and the subsequent cleanup efforts were suggested to inflict acute health problems on soldiers participating in the Hebei Spirit oil spill cleanup activities.

• 한국환경보건학회지
• /
• 제39권2호
• /
• pp.130-137
• /
• 2013

Objectives: The OECD QSAR Application Toolbox was developed by the Organisation for Economic Cooperation and Development (OECD) to facilitate the practical use of QSAR approaches in regulatory contexts as well as to reduce the need for additional animal testing. In this study, human health and the ecotoxicity of chemicals were predicted by applying the OECD QSAR Application Toolbox and the results were compared with experimental data in order to evaluate the applicability of this program. Methods: Read-across, trend analysis, and QSAR of OECD QSAR Application Toolbox were used for the prediction of toxicity. Results: The toxicity prediction was conducted on 6,354 chemicals for which toxicity data have been produced on the six endpoints of skin sensitization, skin irritation, eye irritation, mutagenicity, and acute toxicities of fish and Daphnia. From the total of 6,354, we obtained prediction results for 1,621 chemicals (25.5%). Conclusions: The predicted properties of mutagenicity, skin sensitization, and acute aquatic toxicities were reasonably good when compared with experimental data, but other endpoints were not due to the limitation of applicable chemical groups.