• Environmental Analysis Health and Toxicology
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• 제30권sup호
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• pp.7.1-7.10
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• 2015

Objectives For successful adoption of legislation controlling registration and assessment of chemical substances, it is important to obtain sufficient toxicological experimental evidence and other related information. It is also essential to obtain a sufficient number of predicted risk and toxicity results. Particularly, methods used in predicting toxicities of chemical substances during acquisition of required data, ultimately become an economic method for future dealings with new substances. Although the need for such methods is gradually increasing, the-required information about reliability and applicability range has not been systematically provided. Methods There are various representative environmental and human toxicity models based on quantitative structure-activity relationships (QSAR). Here, we secured the 10 representative QSAR-based prediction models and its information that can make predictions about substances that are expected to be regulated. We used models that predict and confirm usability of the information expected to be collected and submitted according to the legislation. After collecting and evaluating each predictive model and relevant data, we prepared methods quantifying the scientific validity and reliability, which are essential conditions for using predictive models. Results We calculated predicted values for the models. Furthermore, we deduced and compared adequacies of the models using the Alternative non-testing method assessed for Registration, Evaluation, Authorization, and Restriction of Chemicals Substances scoring system, and deduced the applicability domains for each model. Additionally, we calculated and compared inclusion rates of substances expected to be regulated, to confirm the applicability. Conclusions We evaluated and compared the data, adequacy, and applicability of our selected QSAR-based toxicity prediction models, and included them in a database. Based on this data, we aimed to construct a system that can be used with predicted toxicity results. Furthermore, by presenting the suitability of individual predicted results, we aimed to provide a foundation that could be used in actual assessments and regulations.

• 한국콘텐츠학회:학술대회논문집
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• 한국콘텐츠학회 2009년도 춘계 종합학술대회 논문집
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• pp.449-453
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• 2009

본 연구의 목적은 여가제약 협상노력과 레저스포츠참가의 관계를 규명하기 위하여 2009년 2월 9일부터 3월6일까지 26일간 걸쳐서 수도권지역 만 19세의 성인을 대상으로 일반인, 동우회, 클럽회원으로 설정한 다음 유의표집법(purposive Sampling methid)으로 250명을 표본 추출하였다. 본 연구는 레저스포츠참가자활동의 비참가자를 배제하기 위하여 레저스포츠시설들을 방문하여 표본조사를 실시하였다. 수집된 자료는 SPSSWIN15.0 프로그램을 이용하여 요인분석, 신뢰도 분석, 다중회귀분석, 경로분석 등의 방법을 활용하였다. 결론은 다음과 같다. 첫째, 여가제약 협상노력은 레저스포츠 참가 빈도에 영향을 미친다. 즉 여가활동 열망변화 노력이 커질수록 참가 빈도는 높아진다. 둘째, 여가제약 협상노력은 레저스포츠참가 기간에 영향을 미친다. 이는 여가활동 에너지충전을 잘 할수록 강도조절을 잘 할수록 참가 기간이 길어진다. 셋째, 여가제약 협상노력은 레저스포츠참가 강도에 영향을 미친다. 이는 여가활동을 위해 노력이 커질수록 참가 강도가 높아진다.

• 산업경영시스템학회지
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• 제29권3호
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• pp.15-24
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• 2006

As Gyoto Agreement, the Climate Change Agreement, has become effective, total weight restriction on waste has started in each country and environment issue becomes one of the most important global issues. Therefore, environmental logistics became important matter, and industries need to establish production system for environmental conscious products and services. In this paper, we compare and analyze environmental conscious logistics activity(ECLA) that affect Carbon Dioxide Discharge(CDD) and packaging waste caused by logistics activity in Korea and Japan. We also propose the optimal alternative to the problem by this comparison and analysis.

• Journal of Microbiology and Biotechnology
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• 제7권5호
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• pp.293-298
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• 1997

A 4.6-kb HindIII fragment encompassing the complete 140-kDa ${\alpha}$-amylase gene of Lactobacillus amylovorus B 4540 was cloned into pBR322 by the shot gun method. Southern blotting and restriction mapping for the insert were performed. The recombinant 9.0-kb plasmid, pFML1, conferred ${\alpha}$-amylase activity to E. coli and Lactococcus lactis hosts when introduced by electroporation. SDS-PAGE and zymography confirmed the production of 140-kDa ${\alpha}$-amylase and its proteolytic degradation products with enzyme activity in transformants. Total ${\alpha}$-amylase activity of E. coli $DH5{\alpha}$ cells harboring pFML1 was 1.8 units and most activity was detected from cell pellets. Total enzyme activity of L. lactis subsp. lactis MG1363 transformant was five to ten-fold lower than that of E. coli cell but more than half of the activity was detected in the culture supernatant.

• 미생물학회지
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• 제32권3호
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• pp.208-214
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• 1994

토양으로부터 분리한 방선균주로부터 새로운 type II 제한효소를 분리하여 그 특성을 연구하였다. 이 균주는 수리분석 결과, Streptoverticillium olivoverticillatum으로 동정되었으며, 정제한 제한효소는 SolI이라 명명하였다. SoII은 BamHI의 isoschizomer로서 여섯 개의 염기서열 5'-G $\downarrow$ GATCC- 3'을 인지하며 두 개의 G 염기 사이에서 절단하여 4 base가 돌출된 5'말단을 생성한다. 그러나 BamHI과는 달리, dam methylation 되어 있는 인식 염기서열에는 작용하지 못하였다. Ammonium sulfate 분획(30-65%)과 heparin-agarose, Affi-gel Blue column chromatography를 거쳐 SolI을 부분 정제하였다. SolI은 활성을 보이기 위하여 0.2mM 이상의 $MgCl_2$를 반드시 필요로 하였으며, 다른 cofactor는 요구하지 않았다. NaCl이 없을 때 가장 높은 활성을 가지며 120 mM 이상의 NaCl이 있으면 활성이 완전히 억제되었다. 이 효소의 반응 최적 온도는 $40^{\circ}C$, 최적 pH는 8.6으로 나타났다. Gel filtration chromatography에서의 용출부피 비교로부터 이 효소의 분자량은 약 43,000Da인 것으로 추정된다.

• 한국노년학
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• 제30권1호
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• pp.93-108
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• 2010

본 연구는 한국에 거주하는 65세 이상 노인을 대상으로 건강상태가 삶의 질에 영향을 미치는 요인을 알아보기 위해 한국보건사회연구원의 <2005년 국민건강영양조사 제 3기> 원 자료를 이용하여 이차 분석을 실시하였다. 한국보건사회연구원의 조사는 2005년에 실시되었고, 조사된 자료는 총 34,152건이었으며, 이중 65세 이상 노인을 대상으로 조사된 총 3,074건의 자료 중 무응답 항목이 있는 경우와 응답 오류가 있는 경우를 제외한 2,036건을 최종 분석대상으로 하여 다음과 같은 결론을 얻었다. 삶의 질에서 전체 평균 삶의 질은 3점 만점에서 2.61점으로 나타났고, 남자일수록, 연령이 낮을수록, 교육수준이 높을수록, 배우자가 있을수록, 직업이 있을수록, 총 가구소득이 높을수록, 의료보장 종류가 사업장(직장)가입일수록 삶의 질이 높은 것으로 나타났다. 또한 이환일수가 높을수록, 활동제한 년 수가 높을수록, 주관적 건강상태가 나쁠수록 삶의 질이 낮은 것으로 나타났고 삶의 질에 가장 큰 영향을 미치는 것은 주관적 건강상태였다.

• Journal of Photoscience
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• 제9권2호
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• pp.252-254
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• 2002

Djungarian hamsters show distinct seasonal rhythms in several physiological parameters. One of them is daily torpor that occurs in winter with decreased body temperature (about 1O-20$^{\circ}$C) during daytime. Daily torpor is induced by short-day photoperiod, food restriction and castration. But the mechanism to induce daily torpor has not been clarified. In the present study, we tried to clarify the process of daily torpor induction in detail. Adult male hamsters were kept in long photoperiod and high temperature (LP-HT) before the experiment and, thereafter, the animals were transferred to short photoperiod and low temperature (SP-LT), and they were kept in this condition for about six months. The daily rhythms of locomotor activity and body temperature were recorded every three-minutes by using the Minimitter telemetry system. Locomotor activity and body temperature showed very closely synchronized rhythms. All animals under LP-HT showed daily rhythms with higher locomotor activity and body temperature in nighttime than in daytime. Under SP-LT, there were two types of animals with and without showing daily torpor. Thus, they have individual differences in the response to SP -LT.

• The Journal of Korean Physical Therapy
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• 제26권2호
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• pp.130-135
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• 2014

Purpose: The purpose of this study was to examine the impairments of body structures, activity limitation, and participation restriction. In addition, we wanted to provide basic data on correlation between impairments of body structures, activity, and participation in Disabled Persons Living at Home. Methods: After selection of 128 people with physical disabilities more than the third level and brain damage disabilities living at home, we conducted testing for impairments of body structures, activity, and participation, according to the ICF checklist. Results: Impairments of body structures was highest in the upper and lower extremity. 2) Mobility, domestic life, and self care were more limited. 3) Also, the structure related to movement showed correlation with mobility, domestic life, and self care. Conclusion: We observed differences in participation and activities of persons with disabilities Living at Home depending on the impairments of body structures. After thorough review of the status of Disabled Persons Living at Home, we decided to appropriate support and social services.

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 1986년도 추계학술대회
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• pp.525.1-525
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• 1986

To clone ${\beta}$-glucosidase gene from Cellulomonas sp. a gene library was constructed using E. coli JM83 pUC9. Among 2,500 pseudotransformants obtained, 20 clones developed yellow color on the p-nitrophenyl- -D-glucopyranoside filter paper These 20 clones were classified into three groups based on the results of activity staining using nondenaturating polyacrylamide gel electrophoresis and restriction enzyme digestions. Among the three groups, only one group containing pCEl plasmid has specificity for cellobiose.

• 대한치과의사협회지
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• 제12권10호
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• pp.767-770
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• 1974

A 12 years old patient having rampant dental caries on permanent dentition has been treated under operative procedures. In addition to this operative treatment the nutritional survey and the caries activity was checked, and performed the oral hygiene instruction to the patient. As the consequences of above procedures the following results were obtained: 1. The familial history and eating habits checkings were helpful for better dental health guide and caries control. 2. The restriction of mono & disaccharide intake and topical application of 8% stannous fluoride at least two times revealed excellent control effect for recurrent dental caries.