• 한국산업보건학회지
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• 제24권2호
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• pp.208-218
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• 2014

Objectives: The major objective of this study was to develop and validate a tier 1 exposure model utilizing worker exposure monitoring data and characteristics of worker activities routinely performed at construction sites, in order to estimate worker exposures without sampling. Methods: The Registration, Evaluation, Authorization and Restriction of Chemicals(REACH) system of the European Union(EU) allows the usage of exposure models for anticipating chemical exposure of manufacturing workers and consumers. Several exposure models have been developed such as Advanced REACH Tools(ART). The ART model is based on structured subjective assessment model. Using the same framework, a tier 1 exposure model has been developed. Worker activities at construction sites have been analyzed and modifying factors have been assigned for each activity. Korean Occupational Safety and Health Agency(KOSHA) accrued work exposure monitoring data for the last 10 years, which were retrieved and converted into exposure scores. A separate set of sampling data were collected to validate the developed exposure model. These algorithm have been realized on Excel spreadsheet for convenience and easy access. Results: The correlation coefficient of the developed model between exposure scores and monitoring data was 0.36, which is smaller than those of EU models(0.6~0.7). One of the main reasons explaining the discrepancy is poor description on worker activities in KOSHA database. Conclusions: The developed tier 1 exposure model can help industrial hygienists judge whether or not air sampling is required or not.

• Environmental Analysis Health and Toxicology
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• 제30권
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• pp.17.1-17.6
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• 2015

Objectives In this study, the possibility of using existing test data provided in Korea and elsewhere for the registration of chemical substances was examined. Data on 510 chemical substances that are among the first subject to registration under the "Act on the Registration and Evaluation, etc. of Chemical Substances (K-REACH)" were analyzed. Methods The possibility of using existing data from 16 reference databases was examined for 510 chemical substances notified in July 2015 as being subject to registration. Results Test data with the reliability required for the registration of chemical substances under the K-REACH constituted 48.4% of the required physicochemical characteristics, 6.5% of the required health hazards, and 9.4% of the required environmental hazards. Conclusions Some existing test data were not within the scope of this research, including data used for registration in the European Union (EU). Thus, considering that 350 of these 510 species are registered in EU Registration, Evaluation, Authorisation & Restriction of Chemicals, more test data may exist that can be utilized in addition to the data identified in this study. Furthermore, the K-REACH states that non-testing data (test results predicted through Read Across, Quantitative Structure-Activity Relationships) and the weight of evidence (test results predicted based on test data with low reliability) can also be utilized for registration data. Therefore, if methods for using such data were actively reviewed, it would be possible to reduce the cost of securing test data required for the registration of chemical substances.

• Reproductive and Developmental Biology
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• 제33권2호
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• pp.93-98
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• 2009

In this study, bovine Dnmt1 cDNA was sequenced and detected Dnmt1 mRNA level in bovine tissues by northern blot, methylation pattern of genome by southern blot, specific localization of Dnmt1 in mouse and bovine preimplantation embryos by immunocytostaining and Dnmt1 protein level in ovary and testis by western blot. Bovine Dnmt1 cDNA sequence showed more homology with that of human than mouse and rat. The RNA level of Dnmt1 was 10 times higher expression in placenta than other tissues. This indicates that placenta was hypermethylated compared to others organs. The genomic DNA could not be cut by a specific restriction enzyme (HpaII) in placenta, lung and liver of bovine. It suggests that Dnmt1 in some somatic cells was already methylated. Dnmt1, which has the antibody epitope 1316~1616, was distributed in nucleus and cytoplasm including the stage of pronuclear stage and maturation of oocyte and gradually weaken to blastocyst stage compare to negative. In addition, Dnmt1 was strongly expressed in tetraploid embryo and cloned 8-cell than IVF 8-cell. An aberrant pattern of DNA methylation in cloned embryo may be abnormal development of fetus, embryonic lethality and placenta dysfunction. The somatic specific band (190kDa) was appeared in ovary and testis, but oocyte specific band (175kDa) was not. Further investigations are necessary to understand the complex links between the methyltransferases and the transcriptional activity of genes in the cloned bovine tissues.

• Journal of Nutrition and Health
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• 제49권2호
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• pp.72-79
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• 2016

Purpose: The initiation of mandatory folic acid fortification using pteroylmonoglutamic acid (PteGlu) has reduced the rate of congenital malformations. However, it also appears to be responsible for several adverse effects, including increased cancer incidence. This may be related to physicho-chemical characteristics of PteGlu. This study examines the potential effect of high concentrations of PteGlu on a population subjected to mandatory folic acid fortification using an in vitro model. Methods: Caco-2 (colorectal cancer) and MCF7 (breast cancer) cell lines were cultured at 6 different PteGlu concentrations (0, 0.1, 1, 50, 250, and $500{\mu}g/ml$) for 6 days. Cell growth was determined using thiazolyl blue tetrazolium bromide assay. The genotype of dihydrofolate reductase 19bp deletion/insertion (DHFR 19-del) was also scored in cell lines using a restriction fragment length polymorphism technique to examine whether genetic variations may factor in cell proliferation. Results: PteGlu exhibited differential growth promoting properties between cell lines. Caco-2 cells did not show a significant growth difference at low concentrations compared to control, however, at higher concentrations, the growth showed a contrasting trend in the early experimental period, while MCF7 showed enhanced cell growth at all concentrations. The DHFR 19-del genotype differed in the two cell lines. Conclusions: Altered response to PteGlu by Caco-2 and MCF7 may reflect a tissue specific disease aetiology or genotype specific differential enzyme activity, for example by DHFR, to critical levels of PteGlu. As folic acid fortification is a blanket intervention, and DHFR and other enzyme activities vary between individuals, PteGlu intake may have an as yet undefined effect on health. These findings may be relevant when considering mandatory folic acid fortification for disease prevention.

• 한국산학기술학회논문지
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• 제9권5호
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• pp.1354-1358
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• 2008

본 연구에서는 EU의 6대 유해물질(Pb, Hg, Cd, $C^{+6}$, PBB/PBDE) 규제와 국내 환경부의 환경유해물질 규제법규에 대응할 수 있는 친환경 무독성 난연 도료를 개발하였다. 개발한 도료는 VOC(휘발성유기화합물) 배출이 적어 친환경적이며, 할로겐계를 탈피하여 인체안전성 확보됨과 동시에 검정기술기준(KOFEIS 0201)에 적합한 수용성 유 무기계 복합화합물이다. 유 무기계 난연 복합화합물 제조를 위하여 $Mg(OH)_2$, $Sb_{2}O_{3}$, 붕산아연 등의 난연성 화합물을 사용하였으며 각 화합물의 최적배합 비율은 1: 2: 2였다. 최종적으로 난연 복합화합물을 바인더(마이셀 2%) 및 용제(물)와 1: 0.5: 0.5로 배합하여 난연도료를 제조하여 그 난연 성능을 테스트하였다.

• Asian Pacific Journal of Cancer Prevention
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• 제14권12호
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• pp.7629-7634
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• 2013

Background: Cylin D1(CCDN1) is an important regulator of the cell cycle whose alterations are thought to be involved in cancer development. There have been many studies indicating CCDN1 amplification or over-expression in a variety of cancer types. In addition to gene amplification, the G870A polymorphism may be related with altered CCDN1 activity, and therefore with cancer development. This hypothesis has been tested in different cancer types but results have been contradictory. We therefore aimed to investigate any relationship between CCDN1 A870G genotypes and laryngeal squamous cell cancer development and progression. Materials and Methods: A total of 68 Turkish patients with primary laryngeal squamous cell cancer and 133 healthy controls were enrolled. Polymerase chain reaction-restriction fragment length polymorphism analysis was used to determine the CCDN1 genotypes. Results: No significant association was detected between CCDN1 genotypes and laryngeal squamous cell cancer (LxSCCa) development. Similarly CCDN1 genotypes were not related to clinical parameters of Lx SCCa. However, there was a very significant association between CCDN1 G allele and presence of perineural invasion (p=0.003; OR: 1.464; CI% 1.073-1.999). CCDN1 G allele frequency was significantly higher in the individuals with perineural invasion (85.7%) when compared to those without (58.5%). The 2 patients who died of disease were both found to possess the GG genotype. Conclusions: These results pose a controversy in suggesting a protective role of the G allele against LxSCCa development and support the association of CCDN1 gene GG genotype with mortality in patients with LxSCCa.

• 대한간호학회지
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• 제48권3호
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• pp.266-278
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• 2018

Purpose: The aim of this study was to develop a substantive theory on self-management conducted by the adolescents with chronic kidney disease from their lived experience. Methods: Data was collected through in-depth interviews from May to December in 2015 with thirteen adolescents with chronic kidney disease. The data collected were analyzed on the basis of Strauss and Corbin's grounded theory. Results: The core of the category found in this study was "overcoming the unstable sense of self- control and integrating disease experience into their life". The causal conditions triggering the central phenomenon were "restriction in daily life" and "manifestation and aggravation of symptom". The central phenomenon in the experience of self-management within the adolescents with chronic kidney disease was "unstable sense of self control". The intervening condition for unstable self control were "micro system support" and "motivational resources". This study found that the adolescents with chronic kidney disease followed a series of strategies when they faced the central phenomenon, including; passive coping, reappraisal of illness, active coping, compliance with treatment, controlling physical activity, and adjusting school life. With these strategic approaches, the adolescents with chronic kidney disease could maintain their active lifestyles and achieve their health behaviors. The process of self-management by these adolescents passed through four phases; limited experience caused by diseases, effort for normalization, reorganizing their daily lives, and integration with daily lives and self-management. Conclusion: This Study explored the process and experience of self-management of adolescents with chronic kidney disease. These findings can be used for basis for developing substantive theory and nursing intervention strategy for adolescents with chronic kidney diseases.

• 디지털융복합연구
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• 제15권6호
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• pp.283-295
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• 2017

본 연구는 2013년도 한국의료패널 자료를 이용하여 에코세대의 정신건강 및 신체건강이 건강관련 삶의 질에 미치는 융복합적 영향을 파악하기 위하여 실시되었다. 2013년도 한국의료패널 자료는 2013년 2월에서 10월까지 조사되었고, 분석대상자는 2,261명이었다. 수집된 자료는 SPSS WIN Version 24.0을 이용하여 기술통계, Independent t-test, ANOVA, 위계적 중회귀분석을 실시하였다. 연구결과, 에코세대의 건강관련 삶의 질 평균은 0.98점이었다. 에코세대 건강관련 삶의 질 영향요인은 미충족의료 유무, 정신적 신체적 스트레스 유무, 기본적 욕구 미충족 유무, 미래에 대한 불안 유무, 우울감 유무 및 자살생각 유무, 흡연 여부, 주중 수면시간, 청력문제 유무, 섭식문제 유무, 활동제한 유무 및 주관적 건강상태로 나타났다. 에코세대의 건강관련 삶의 질을 향상시키기 위하여 에코세대의 정신건강 및 신체건강의 특성을 반영한 건강관리프로그램을 개발하고 지역사회 연계 체계를 구축하여 지속적인 상담과 교육이 필요하다.

• 성인간호학회지
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• 제16권3호
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• pp.398-408
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• 2004

Purpose: The purpose of this study was to identify the frequency of urinary incontinence and lower urinary symptoms, to identify lifestyle and sexual matters for UI of adult women in an urban area. Method: The sample consisted of 364 educated women from a women's health education program in a public health center. For data collection, the Bristol Female Lower Urinary Tract Symptoms Questionnaire developed by Jackson, et. al.(1996) and the demographic questionnaire were used. For data analysis, SAS 8.2 program was used. Result: The results were as follows: 1. the prevalence rate of UI was 73.63%. 45.88% of them were stress incontinence and 27.75% of them were urge incontinence. 2. Remarkable differences were found between the type of UI and LUTS in the case of urgency($x^2=42.0585$, P<0.001), unexplained incontinence($x^2=59.0585$, P<0.001), noctural incontinence($x^2=18.8080$, P<0.001) and catetherization($x^2=10.7207$, P= 0.004), burning sense($x^2=27.7400$, P<0.001). 3. Remarkable differences were found between the type of UI and lifestyle matters in the cases as follows fluid intake restriction($x^2=31.0532$, P=0.008), interference in physical activity($x^2=36.7481$, P=0.001), interference in relation with others($x^2=22.2729$, P=0.034). 4. Remarkable differences were found between the type of UI and sexual matters with difficulty of sexual intercourse($x^2=16.1898$, P=0.002), and urine leakage during sexual intercourse($x^2=17.9752$, P=0.001). Conclusion: Based on the results, developing and carrying out an adequate UI care and education program is needed.

• 한국수소및신에너지학회논문집
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• 제19권3호
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• pp.199-208
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• 2008

HI decomposition was conducted using Pt/C-based catalysts with a fixed-bed reactor in the range of 573 K to 773 K. To examine the change of the characteristic properties of the catalysts, $N_2$ adsorption analyser, a X-ray diffractometer(XRD), and a scanning electron microscopy(SEM) were used before and after the HI decomposition reaction. the effect of Pt loading on HI decomposition was investigated by $CO_2$-TPD. HI conversion of all catalysts increased as decomposition temperature increased. The XRD analysis showed that the sizes of platinum particle became larger and agglomerated into a lump during the reaction. From $CO_2$-TPD, it can be concluded that the cause for the increase in catalytic activity may be attributed to the basic sites of catalyst surface. The results of both b desorption and gasification reaction showed the restriction on the use of Pt/C-based catalyst.