• 제목/요약/키워드: Activity ratio

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Comparison of Milk-clotting Activity of Proteinase Produced by Bacillus Subtilis var, natto and Rhizopus oligosporus with Commercial Rennet

  • Chen, Ming Tsao;Lu, Ying Yu;Weng, Tien Man
    • Asian-Australasian Journal of Animal Sciences
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    • 제23권10호
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    • pp.1369-1379
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    • 2010
  • This study investigated purification and milk-clotting activity of the enzymes produced by Bacillus subtilis var, natto and Rhizopus oligosporus compared with that of commercial rennet. The clotting time, viscosity, tension and microstructure of the curd and electrophoretic patterns of milk proteins were determined. The milk-clotting activity/proteolytic activity ratios (MCA/PA ratio) of B. subtilis, R. oligosporus and commercial rennet were also compared. The results revealed that the curd formed by the commercial rennet had the highest viscosity and curd tension and the shortest clotting time among the three enzymes. However, curd produced by Rhizopus enzymes was ranked as second. From the MCA/PA ratio and electrophoretogram analyses it could be concluded that the enzyme produced by B. subtilis had the highest proteolytic activity, while the commercial rennet had the highest milk-clotting activity. Observations of microstructures of SEM showed that the three-dimensional network for curd formed by commercial rennet was denser, firmer and more smooth. The milk-clotting activity, specific activity, purification ratio and recovery of the purified enzymes produced by both the tested organisms were also determined with ion exchange chromatography and gel filtration.

Pilocarpine이 토끼 적혈구막의 NaK ATPase의 활성도에 대한 작용 (Action of Pilocarpine on Sodium-Potassium activated ATPase in Rabbit Red Cell Membrane)

  • 고일섭
    • The Korean Journal of Physiology
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    • 제11권1호
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    • pp.11-20
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    • 1977
  • The action of pilocarpine on the sodium plus potassium activated ATPase activity in the rabbit red cell membrane has been investigated and the experiments were also designed to determine the mechanism of action of pilocarpine on the ATPase activity. The following results were observed. 1. The activity of the NaK ATPase from red cell membrane is stimulated by pilocarpine, and the concentration of pilocarpine for maximal activity is about 3 mM. The pH optimum for the pilocarpine sensitive component is 8.0. 2. The activating effect of pilocarpine on the ATPase, with a given concentration of sodium .in the medium, is increased by raising the potassium concentration but activity ratio is decreased 3. The activating effect of pilocarpine on the ATPase, with a given concentration of Potassium in the medium, is increased by raising the sodium concentration but activity ratio is decreased 4. The NaK ATPase activity is increased by small amounts of calcium but decreased by 'larger amounts. The activity ratio of the enzyme by pilocarpine is decreased by small amounts .of calcium but decreased by larger amounts. 5. The activating effect of pilocarpine on the ATPase was not related to the sulfhydryl group of cysteine, the hydroxyl group of threonine or the imidazole group of histidine. 6. The activating effect of pilocarpine on the ATPase is due to amino group and carboxyl group of the enzyme of NaK ATPase

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클램 운동 시 운동범위에 따른 엉덩관절 벌림근의 활성도와 사용 비교 (Comparison of Activity and Use of Hip Abductor Muscle Group According to Range of Motion during the Clam Exercise)

  • 최용길;이상열;최수홍;윤성영;김인균
    • PNF and Movement
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    • 제20권3호
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    • pp.341-349
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    • 2022
  • Purpose: The purpose of this study is to compare the muscle activity of the hip abductors and the ratio between the hip abductor muscle group according to the range of hip abduction during the clam exercise. Methods: This study was conducted on 18 healthy men in their aged 20 to 29 who had not been diagnosed with spine-related diseases. The subjects performed a clam exercise without rotation of the pelvis in a state of 60° hip flexion and 90° knee joint flexion in the side-lying position. Using Myomotion equipment and EMG, the muscle activity of the hip abductor muscles and the activity ratio between the hip abductor muscle group were measured during the clam exercise by dividing the range into initial, mid-range, and terminal sections. Repeated measures analysis of variants was employed to compare the activity and use of hip abductor muscles according to range of motion during the clam exercise. Results: Gluteus medius muscle activation was significantly increased in the comparison of muscle activity in the initial, mid-range, and terminal sections of hip abduction. Tensor fasciae latae muscle activation was significantly increased in the comparison of muscle activity in all range of motion sections as well. The gluteus medius-tensor fasciae latae muscle activation ratio was significantly increased in the terminal section compared to the initial section. Conclusion: The gluteus medius and tensor fasciae latae had higher muscle activities as they approached the terminal section during the clam exercise, and the hip abduction activity ratio of the gluteus medius and tensor fasciae latae was higher as the range of motion approached the terminal section.

친환경농업 생산자조직의 경영실태 분석과 시사점 (Issues and Analyses on Management Situations of Environment-Friendly Agricultural Organizations)

  • 김호
    • 한국유기농업학회지
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    • 제26권4호
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    • pp.599-607
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    • 2018
  • This study analyzed the management situation of two organizations that have produced environmentally friendly agricultural and livestock products. One organization sells and processes environmentally friendly agricultural products like as grains, vegetables, fruits and processing foods. Another organization processes and sells environmentally friendly livestock products, specially beef products. Indices of management analysis are stability ratio, activity ratio and profitability ratio. Stability ratio indices are debt ratio, net worth ratio, fixed ratio and current ratio. Activity ratio ones include fixed assets turnover and net worth turnover. And profitability ratio is showed through return on investment, net return on sales and return on equity.

친환경농업 A영농법인과 B농업회사법인의 경영실태 분석- 충남 아산의 친환경 생산자조직을 사례로 - (Analyses on Management Situations of 'A' and 'B' Corporations as the Environment-Friendly Agricultural Organization at Asan)

  • 김호
    • 한국유기농업학회지
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    • 제30권4호
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    • pp.471-483
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    • 2022
  • This study analyzed management situations of two organizations that have produced environment-friendly agricultural and processed meat products. 'A' farming corporation sells environment-friendly agricultural products like as grains, vegetables, fruits and processing foods. 'B' agricultural corporation processes and sells environment-friendly processed meat products, specially organic and antibiotic-free beef products. Recently, members and production areas of 'A' farming corporation have decreased because of their aging and labor shortage. And Indices for the management analysis are stability ratio, activity ratio and profitability ratio. Stability ratio indices are debt ratio, net worth ratio, fixed ratio and current ratio. Activity ratio ones include fixed assets turnover and net worth turnover. And profitability ratio is showed through return on investment, net return on sales and return on equity.

말씨와 활동성의 체질특성 문항에 대한 통계적 분석 (A Statistical Analysis of the questionnaire concerning Sasang Constitutional Characteristics on 'Pattern of speech and activity')

  • 문성택;이시우;김홍기;김종열
    • 한국한의학연구원논문집
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    • 제13권1호통권19호
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    • pp.85-92
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    • 2007
  • To evaluate the suitability and effectiveness of the questionnaire concerning personal properties on 'pattern of speech and activity' according to the Sasang constitution that were used in Iksan Wonkwang Oriental Medicine, we analyzed the data of 1,335 patients obtained through the electronic chart in the aspect of 'relative discrimination ability' to Sasang constitutions and 'response ratio' using statistical Package SPSS. In categories of 'speech pattern', No.2 (speak mildly and softly) was effectively discriminating Soeum type. No.4 (talkative) and No.7 (speak fast) were effective factors for the discrimination of Soyang type, though No.4 (talkative) was needed to be improved in response ratio. The category of 'activity pattern' has shown high response ratio but low discriminating power. However, No.2 (keep staying home but avoid going out) in this category was effectively discriminating Soeum type. The discriminating power of 'pattern of speech and activity' for the age group less than 20 years old was too low, so it is necessary to develop the questionnaire for the elementary to high school students as well as for the preschoolers.

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Greening에 따른 유채 자엽의 엽록소-단백질 복합체 형성 (Formation of Chlorophyll-Protein Complexes in Greening Rape Cotyledons)

  • 이진범
    • Journal of Plant Biology
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    • 제26권2호
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    • pp.91-99
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    • 1983
  • The formation of chlorophyll-protein complexes (CP-complexes) during the greening of rape cotyledons (Brassica napus cv. Yongdang) was investigated by the SDS-polyacrylamide gel electrophoresis. The total chlorophyll content and Chl a/b ratio were also determined. In addition, the effects of dark treatment on the CP-complex patterns during greening have been examined with respect to their photosynthetic electron transport activity. Greening has brought about the increasein total chlorophyll content and the decrease in Chl a/b ratio, but there have been no changes in Chl a/b ratio after 24 hrs of greening. The light-harvesting chlorophyll a/b-protein complex (LHCP-complex0 was predominant during the initial greening period. Thereafter, the amout of chlorophyll a-protein complex (CP I-complex) was gradually increased. Twenty-four-hr dark treatment immediately after illumination for 6 hrs and 12 hrs resulted in the increase of the Chl a/b ration and the CP I complex, otherwise the decrease of the LHCP-complex. The LHCP/CP I ratio was gradually decreased with further greening, and appeared no change after 48 hrs illumination. The investigation of the photosynthetic electron transport activity indicated that photosystem (PS) II activity (H2Olongrightarrowp-PD*+FeCy**) did not change, but the activity of PS I was increased suddenly due to the dark treatment. The data suggests that the increase of CP I-complex may result in that of P-700, that is, the increase of PS I activity.

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Immediate Effects of Strain-Counterstrain Technique on Pressure Pain Threshold and Muscle Activity in Male Adults With Upper Trapezius Latent Trigger Point

  • Jung, Ji-Hee;Lee, Nam-Gi;You, Sung-Hyun
    • 한국전문물리치료학회지
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    • 제18권2호
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    • pp.76-83
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    • 2011
  • The aim of this study was to determine the immediate effects of single treatment of strain-counter strain (SCS) on pressure pain threshold (PPT) and muscle activity during scapular plane abduction with 3% body weight load. Fifteen asymptomatic male adults with upper trapezius latent trigger point (LTrP) (PPT<2.9 $kg/cm^2$) participated in this study. Pressure algometer was used to measure PPT and surface electromyography was used to record upper, middle arid lower trapezius, serratus anterior, infraspinatus and middle deltoid muscle activity and relative ratio during scapular plane abduction between pre- and post-intervention. There was a significant increase in upper trapezius PPT after a 90-second SCS (p<.05). The activity of the upper trapezius and middle deltoid was significantly decreased (p=.014, p=.001), coupled with a decreased muscle activity ratio between the upper and lower trapezius (p<.05). These results indicate that the SCS may effectively deactivate upper trapezius activity, thereby alleviating muscle balance and reducing pain sensitivity.

어깨관절벌림 각도에 따른 어깨주변 근육들의 참여 비율에 대한 연구 (The Study of Participation Ratio on Shoulder Abductor Muscle Activities According to Shoulder Abduction Angle)

  • 박민철;이상열
    • 대한통합의학회지
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    • 제5권2호
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    • pp.19-23
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    • 2017
  • Purpose: The purpose of this study was to determine the muscle activity ratio according to the shoulder abduction angle by identifying the mean muscle activities and calculating the muscle ratios for use in developing strengthening methods. Methods: The participants were healthy adults in their 20s (n=19). The activity of the deltoid middle, upper trapezius, middle trapezius, and lower trapezius muscles was measured by 8-channel surface electromyography. Muscle activity was measured during 4 conditions of angle of shoulder abduction: $30^{\circ}$, $60^{\circ}$, $90^{\circ}$, and $120^{\circ}$. The data used in the analysis were the root mean square and % total muscle activity values. Results: The root mean square values for the deltoid middle, upper trapezius, middle trapezius, and lower trapezius muscles showed significant differences. No significant differences were detected in the % total muscle activity of the deltoid middle, upper trapezius, middle trapezius, or lower trapezius muscles. Conclusion: Future studies aimed at developing selective shoulder abductor muscle strengthening methods are likely to provide more effective results by using muscle activity ratios.

주목 세포 현탁배양 중, FCW/DCW ratio, 단백질 생산 및 peroxidase활성 조사 (Monitoring of FCW/DCW ratio, Production of Protein and Peroxidase Activity During Suspension Culture of Taxus chinesis)

  • 최형균;윤정환;김상익;송재영;김진현;최호준;홍승서
    • KSBB Journal
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    • 제15권5호
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    • pp.525-528
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    • 2000
  • Taxus chinensis 세포배양 중의 FCW/DCW ratio, 세포내, 외 단백질 생산 및 peroxidase 활성의 변화를 배양시기별로 조사하였다. 조사 결과, 배지내 잔류당이 고갈되기 직전인 배양 14일째에 FCW/DCW ratio 는 12로 가장 낮은 수치를 나타내었고 DCW를 기준으로 한 세포 외에서의 protein 생산도 14일째 가장 낮은 수치인 4.3 mgfg DCW 였다. 배양시기별 P POD 활성도는 단백질 생산 pattern과 유사하였다. 이 결과들 은 flask에서 활발하게 증식 중인 세포들을 이용하여 측정한 것으로 대량배양시, 공정제어를 위한 자료 및 세포가 받는 stress 정도를 나타내는 지표로 활용할 수 있다

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