• Proceedings of the KOR-KST Conference
• /
• 2007.05a
• /
• pp.128-136
• /
• 2007

• Asian-Australasian Journal of Animal Sciences
• /
• v.31 no.3
• /
• pp.449-456
• /
• 2018

Objective: Nanog, a homeodomain protein, has been investigated in humans and mice using embryonic stem cells (ESCs). Because of the limited availability of ESCs, few studies have reported the function and role of Nanog in porcine ESCs. Therefore, in this study, we investigated the location of the porcine Nanog chromosome and its basal promoter activity, which might have potential applications in development of ESCs specific marker as well as understanding its operating systems in the porcine. Methods: To characterize the porcine Nanog promoter, the 5'-flanking region of Nanog was isolated from cells of mini-pig ears. BLAST database search showed that there are two porcine Nanog genomic loci, chromosome 1 and 5, both of which contain an exon with a start codon. Deletion mutants from the 5'-flanking region of both loci were measured using the Dual-Luciferase Reporter Assay System, and a fluorescence marker, green fluorescence protein. Results: Promoter activity was detected in the sequences of chromosome 5, but not in those of chromosome 1. We identified the sequences from -99 to +194 that possessed promoter activity and contained transcription factor binding sites from deletion fragment analysis. Among the transcription factor binding sites, a Sp1 was found to play a crucial role in basal promoter activity, and point mutation of this site abolished its activity, confirming its role in promoter activity. Furthermore, gel shift analysis and chromatin immunoprecipitation analysis confirmed that Sp1 transcription factor binds to the Sp1 binding site in the porcine Nanog promoter. Taken together, these results show that Sp1 transcription factor is an essential element for porcine Nanog basal activity the same as in human and mouse. Conclusion: We showed that the porcine Nanog gene is located on porcine chromosome 5 and its basal transcriptional activity is controlled by Sp1 transcription factor.

• Journal of Korean Academy of Nursing
• /
• v.45 no.3
• /
• pp.329-336
• /
• 2015

Purpose: The purpose of the study was to identify factors influencing physical activity among community-dwelling older adults with type 2 diabetes. The study design was based on the Theory of Triadic Influence. Methods: A total of 242 older adults with type 2 diabetes participated in this study. Six variables related to physical activity in older adults, including self-efficacy, social normative belief, attitudes, intention, experience, and level of physical activity, were measured using reliable instruments. Data were analyzed using descriptive statistics, Pearson's correlation analyses, and a path analysis. Results: The mean physical activity score was 104.2, range from zero to 381.21. The path analysis showed that self-efficacy had the greatest total effect on physical activity. Also, experience had direct and total effects on physical activity as well as mediated the paths of social normative beliefs to attitudes and intention to physical activity. These factors accounted for 10% of the total variance, and the fit indices of the model satisfied the criteria of fitness. Conclusion: The findings of the study reveal the important role of self-efficacy and past experience in physical activity in older adults with type 2 diabetes.

• Korean Journal of Health Education and Promotion
• /
• v.33 no.3
• /
• pp.95-107
• /
• 2016

Objectives: Physical activity is well known as one of the most effective health behaviors for maintaining and promoting health. The purpose of this study was to investigate the regional variation, compositional and contextual factors on physical activities in Korea. Methods: This study analyzed data of 6,353 adults in 4th-1, 2 Korea National Health and Nutrition Examination Survey (KNHANES). Independent variables were compositional factors that included general characteristics, health behaviors, health status, and contextual factors that included physical environment, political environment, and social safety net. Dependent variables were vigorous physical activity, moderate physical activity, and walking activity. Multilevel analysis were used to determine a variation of physical activity between levels of compositional factors and contextual factors. Results: Physical activities of the Korean people were affected by the regional variables. Financial independence and population density were related with moderate physical activity significantly. Population density, school sport or rally spaces, cultural facilities, and social safety concerns were related with walking activity significantly. These variables impacted on physical activities along with variables at the individual level. Variance Partition Coefficient were 9.94% at moderate physical activity and 2.91% at walking activities, respectively. Conclusions: The results suggest that the regional variables should be considered when planning public intervention to promoting physical activities in Korean adults.

• Journal of Fisheries and Marine Sciences Education
• /
• v.24 no.4
• /
• pp.516-527
• /
• 2012

This study analyzed the programs offered in the youth center located in Gyeongsangnam-do Province in accordance with the domains for creative activity. The creative activity, introduced through the 2009 revised national curriculum, puts an emphasis on the experiential activities to enhance the creativity of students. The creativity activity needs to be operated as intended in order to ensure that the students can grow into adults, who have creativity and good personality, by amassing a variety of experiences. For that, various human resources and physical resources of communities need to be utilized efficiently. Thus, this study explored the measures to achieve the original objectives of creativity activity by analyzing the programs - offered by the youth centers in Gyeongsangnam-do Province - based on the scope of creativity activity. The followings are the results of the analysis of 177 programs offered in 12 organizations that came under the three categories (training facilities operated directly by the Education Office, public training centers, culture center) of the youth centers located in Gyeongsangnam-do Province. Autonomous activity' comprised the highest proportion in the youth center programs of the three categories, followed by club activity. The programs associated with the 'career activity' - which most students have the highest expectations in relation to the creativity activity - comprised relatively low proportion. In conclusion, the programs offered by the youth centers in communities need to become more diversified in order to achieve the intended results of creative activity. In addition, the programs associated with the 'career activity', which student prefer the most, need to be operated in a more diversified manner with greater emphasis. Furthermore, the measures should be explored which may increase the involvement of youth education experts of community in the operations of creativity activity.

• Journal of Korean Public Health Nursing
• /
• v.32 no.1
• /
• pp.56-68
• /
• 2018

Purpose: This study was conducted to examine the personal and social environmental factors affecting leisure time physical activity (LTPA) among adults living in rural communities. Methods: A cross-sectional study was conducted with a convenience sample of 282 adults living in three rural towns of Chungcheongbuk province. Leisure time physical activity was assessed through the Korean version of the Global Physical Activity Questionnaire (GPAQ), and social influence and social cohesion of social environment were assessed using reliable and validated measures. Multiple logistic regression analysis was conducted to explore personal and social environmental factors associated with LTPA. Results: The overall prevalence of leisure-time physical activity (LTPA) was 40.0%. Multiple logistic regression analysis showed that LTPA participation was associated with gender (OR=2.60, 95% CI=1.47-4.58), healthy eating (OR=1.15, 95% CI=1.01-1.31), unhealthy eating (OR=0.70, 95% CI=0.53-0.93) and social influence for physical activity (OR=1.11, 95% CI=1.03-1.20). However, social cohesion was not correlated with engaging in LTPA. Conclusion: Both personal and social environmental factors are associated with LPTA among rural adults living in Eup-Myen regions. Interventions to promote LTPA would likely benefit from considering healthy dietary intake and social influence for physical activity.

• Journal of Korean Society for Quality Management
• /
• v.46 no.3
• /
• pp.551-568
• /
• 2018

Purpose: This study addressed the relationship between technological innovation activity and management efficiency of Korean automobile firms. We tested the hypothesis of non-liner relationship of innovation activity in relation to management efficiency. Methods: We discussed prior literature in the firm innovation strategy and management efficiency studies to provide better understanding of relationships between technological innovation activity and management efficiency. As a result, we developed develop and tested a model (Inverted-U shaped) capturing the non-linear impact of technological innovation activity. While we used R&D expenditure and patent registration data for measuring firms' innovation activity, management efficiency was evaluated by using DEA(Data Envelopment Analysis). Results: Main findings of our empirical analysis indicated that the relationships between technological innovation activity and management efficiency was inverted U shaped. This implied that the relationship between technological innovation and management efficiency is inverted U-shaped non-linear, with management efficiency increasing up to a point, beyond which higher levels of R&D and patent registration activities led to a decrease in management efficiency. Conclusion: This study empirically assessed the inconclusive findings of previous research in the area of effects of innovation activities in relation to firm performance. The paper also provided theoretical and practical implications for firms who explore efficient strategy to promote the management performance through technological innovation activities. Future research directions with the limitation of the study was discussed.

• Environmental Analysis Health and Toxicology
• /
• v.15 no.4
• /
• pp.147-155
• /
• 2000

In order to evaluate anti -oxidant activities and protective effect against oxidatve damage, DPPH radical scavenging activity and lipid peroxidation inhibitory activity were measured among methanol extracts prepared from natural medicinal plants. Fourteen natural medicinal plants which were reported to have anti -oxidative or anti-inflammatory effects were selected based on our previous report. In addition to the total methanol extracts, n-hexane, dichloromethane, ethylacetate, n-butanol and water fractions were prepared from each total extract. DPPH radical scavenging assay was performed against 14 total extracts and all samples showed dose-dependent activities in various extent. Among those, 6 samples, methanol extracts of Euryale ferox, paeonia suffruticosa, Areca catechu var. dulcissima, Cinnamomun cassia, Alpinia katsumadai and Betula platyphlla var. japonica showed IC$\sub$50/ value lower than 6.0 $\mu\textrm{g}$/ml. The highest DPPH radical scavenging activity was found in ethylacetate fraction of paeonia suffruticosa with IC$\sub$50/ value of 1.1 $\mu\textrm{g}$/ml. Analysis of lipid peroxidation inhibitory activity on hydrogen peroxide-induced oxidative damage in Chinese hamster lung fibroblast (V79-4) cells revealed that the highest inhibitory effect was observed in methanol extract of Betula platyhpylla var. japonica. Lipid peroxidation inhibitory activity was observed as a dose-dependent manner in all samples used in this study. Among fraction samples, ethylacetate fraction of Alpinia katsumadai had the strongest inhibitory activity with IC$\sub$50/ value of 0.9 $\mu\textrm{g}$/ml.

• Transactions of the Korean hydrogen and new energy society
• /
• v.14 no.1
• /
• pp.1-7
• /
• 2003

In this study, activity changes of $Ni/Ce-ZrO_2$ catalyst for steam reforming reaction in the various steam treatment condition were investigated and BET, XRD and XPS analysis were introduced to characterize the catalyst before and after treatment. Activity test showed that $Ni/Ce-ZrO_2$ catalyst had good activity after reduction in steam reforming reaction but deactivated rapidly after steam treatment at high temperature. Activities of deactivated catalyst by steam was recovered to die previous activity level after reduction using hydrogen rich gas. It was observed that catalytic activity was preserved after repeated steam treatment, too. It showed that change of catalytic activity due to steam treatment is perfectly reversible. From the BET, XRD and XPS analysis, deactivation of $Ni/Ce-ZrO_2$ catalyst was due to the transition from Ni, that is activity site for steam reforming reaction, to $NiAl_2O_4$ in steam treatment at high temperature.

• Journal of Korean Public Health Nursing
• /
• v.27 no.1
• /
• pp.153-165
• /
• 2013

Purpose: The purpose of this study was to identify the relationships between physical activity, health status, and quality of life (QOL) of University students. Methods: The research design was based on a descriptive research design model. This study sample consisted of 183 K University students in D city. Data was collected from December 2012 to January 2013 and analyzed with descriptive statistics (frequency, percentage, analysis of variance (ANOVA), t-test, and Pearson's correlation analysis) using SPSS 19.0. Subjects completed questionnaires on their demographics, physical activity, health status, and QOL. Results: The overall physical activity of participants was $2529.40{\pm}5057.896$ min/week on average. The average health status was $28.66{\pm}6.3$ and the average physical health and mental health were $16.45{\pm}3.955$ and $12.31{\pm}3.446$, respectively. The overall degree of QOL was $3.429{\pm}0.379$. A positive relationship existed between moderate and vigorous activity and health status. In addition, QOL positively correlated with physical health (under health status). However, physical activity was not related with both mental health (under health status) and QOL. Conclusion: Based on the results of this study, health educational programs are needed to promote sufficient physical activity in university students.